Details for: MED16

Gene ID: 10025

Symbol: MED16

Ensembl ID: ENSG00000175221

Description: mediator complex subunit 16

Associated with

Cells (max top 100)

(Marker Scores and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: germ cell (CL0000586)
    Fold Change: 2.05
    Marker Score: 3,587
  • Cell Name: oocyte (CL0000023)
    Fold Change: 1.77
    Marker Score: 438
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 1.33
    Marker Score: 5,215
  • Cell Name: acinar cell (CL0000622)
    Fold Change: 1.11
    Marker Score: 782
  • Cell Name: Unknown (CL0002371)
    Fold Change: 1.11
    Marker Score: 1,176
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: 1.03
    Marker Score: 701
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71,701
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 47,925
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30,394
  • Cell Name: respiratory epithelial cell (CL0002368)
    Fold Change: 0.97
    Marker Score: 533
  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 0.95
    Marker Score: 1,366
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.94
    Marker Score: 2,394
  • Cell Name: ovarian surface epithelial cell (CL2000064)
    Fold Change: 0.94
    Marker Score: 2,559
  • Cell Name: Leydig cell (CL0000178)
    Fold Change: 0.94
    Marker Score: 1,009
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.93
    Marker Score: 478
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.92
    Marker Score: 434
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.9
    Marker Score: 2,698
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.86
    Marker Score: 5,228
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.85
    Marker Score: 306
  • Cell Name: foveolar cell of stomach (CL0002179)
    Fold Change: 0.85
    Marker Score: 5,426
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.84
    Marker Score: 1,235
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.83
    Marker Score: 4,747
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: 0.82
    Marker Score: 856
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: 0.82
    Marker Score: 8,250
  • Cell Name: epithelial cell of nephron (CL1000449)
    Fold Change: 0.82
    Marker Score: 1,706
  • Cell Name: mammary gland epithelial cell (CL0002327)
    Fold Change: 0.82
    Marker Score: 290
  • Cell Name: interstitial cell of ovary (CL0002094)
    Fold Change: 0.82
    Marker Score: 5,308
  • Cell Name: endocrine cell (CL0000163)
    Fold Change: 0.8
    Marker Score: 416
  • Cell Name: kidney cell (CL1000497)
    Fold Change: 0.78
    Marker Score: 521
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1,263
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.76
    Marker Score: 579
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 0.75
    Marker Score: 1,002
  • Cell Name: blood cell (CL0000081)
    Fold Change: 0.75
    Marker Score: 8,705
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.74
    Marker Score: 298
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: 0.74
    Marker Score: 779
  • Cell Name: DN3 thymocyte (CL0000807)
    Fold Change: 0.73
    Marker Score: 367
  • Cell Name: kidney collecting duct intercalated cell (CL1001432)
    Fold Change: 0.73
    Marker Score: 1,210
  • Cell Name: primitive red blood cell (CL0002355)
    Fold Change: 0.73
    Marker Score: 546
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.72
    Marker Score: 374
  • Cell Name: kidney collecting duct principal cell (CL1001431)
    Fold Change: 0.72
    Marker Score: 1,817
  • Cell Name: enterocyte (CL0000584)
    Fold Change: 0.72
    Marker Score: 3,445
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.71
    Marker Score: 24,669
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.71
    Marker Score: 743
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.7
    Marker Score: 1,184
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.69
    Marker Score: 443
  • Cell Name: bronchial goblet cell (CL1000312)
    Fold Change: 0.69
    Marker Score: 341
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.68
    Marker Score: 682
  • Cell Name: preosteoblast (CL0007010)
    Fold Change: 0.68
    Marker Score: 192
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.67
    Marker Score: 172
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.67
    Marker Score: 2,805
  • Cell Name: ependymal cell (CL0000065)
    Fold Change: 0.67
    Marker Score: 232
  • Cell Name: erythroid progenitor cell, mammalian (CL0001066)
    Fold Change: 0.66
    Marker Score: 213
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: 0.66
    Marker Score: 22,483
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.66
    Marker Score: 599
  • Cell Name: respiratory goblet cell (CL0002370)
    Fold Change: 0.65
    Marker Score: 189
  • Cell Name: kidney loop of Henle thin ascending limb epithelial cell (CL1001107)
    Fold Change: 0.65
    Marker Score: 661
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 0.65
    Marker Score: 4,126
  • Cell Name: colon epithelial cell (CL0011108)
    Fold Change: 0.64
    Marker Score: 2,022
  • Cell Name: epithelial cell of glomerular capsule (CL1000450)
    Fold Change: 0.64
    Marker Score: 164
  • Cell Name: Unknown (CL0000548)
    Fold Change: 0.64
    Marker Score: 468
  • Cell Name: male germ cell (CL0000015)
    Fold Change: 0.64
    Marker Score: 185
  • Cell Name: intestine goblet cell (CL0019031)
    Fold Change: 0.64
    Marker Score: 609
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 0.62
    Marker Score: 779
  • Cell Name: epithelial cell of proximal tubule (CL0002306)
    Fold Change: 0.62
    Marker Score: 2,200
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.62
    Marker Score: 1,304
  • Cell Name: precursor B cell (CL0000817)
    Fold Change: 0.62
    Marker Score: 408
  • Cell Name: double-positive, alpha-beta thymocyte (CL0000809)
    Fold Change: 0.62
    Marker Score: 1,208
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.61
    Marker Score: 152
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.61
    Marker Score: 1,394
  • Cell Name: CD4-positive helper T cell (CL0000492)
    Fold Change: 0.61
    Marker Score: 659
  • Cell Name: kidney loop of Henle thick ascending limb epithelial cell (CL1001106)
    Fold Change: 0.6
    Marker Score: 1,621
  • Cell Name: keratocyte (CL0002363)
    Fold Change: 0.6
    Marker Score: 139
  • Cell Name: hematopoietic stem cell (CL0000037)
    Fold Change: 0.6
    Marker Score: 317
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 0.6
    Marker Score: 6,087
  • Cell Name: megakaryocyte (CL0000556)
    Fold Change: 0.6
    Marker Score: 341
  • Cell Name: mucous neck cell (CL0000651)
    Fold Change: 0.6
    Marker Score: 1,356
  • Cell Name: Sertoli cell (CL0000216)
    Fold Change: 0.6
    Marker Score: 3,542
  • Cell Name: myoepithelial cell of mammary gland (CL0002324)
    Fold Change: 0.59
    Marker Score: 2,883
  • Cell Name: hematopoietic cell (CL0000988)
    Fold Change: 0.59
    Marker Score: 390
  • Cell Name: basal cell (CL0000646)
    Fold Change: 0.59
    Marker Score: 757
  • Cell Name: decidual cell (CL2000002)
    Fold Change: 0.59
    Marker Score: 2,528
  • Cell Name: type A enteroendocrine cell (CL0002067)
    Fold Change: 0.59
    Marker Score: 236
  • Cell Name: luminal epithelial cell of mammary gland (CL0002326)
    Fold Change: 0.58
    Marker Score: 1,028
  • Cell Name: type EC enteroendocrine cell (CL0000577)
    Fold Change: 0.58
    Marker Score: 546
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: 0.58
    Marker Score: 240
  • Cell Name: paneth cell (CL0000510)
    Fold Change: 0.58
    Marker Score: 402
  • Cell Name: stem cell (CL0000034)
    Fold Change: 0.58
    Marker Score: 1,372
  • Cell Name: glandular cell of esophagus (CL0002657)
    Fold Change: 0.57
    Marker Score: 182
  • Cell Name: cortical thymic epithelial cell (CL0002364)
    Fold Change: 0.57
    Marker Score: 2,115
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.57
    Marker Score: 262
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.57
    Marker Score: 136
  • Cell Name: extravillous trophoblast (CL0008036)
    Fold Change: 0.57
    Marker Score: 508
  • Cell Name: Hofbauer cell (CL3000001)
    Fold Change: 0.57
    Marker Score: 313
  • Cell Name: double negative T regulatory cell (CL0011024)
    Fold Change: 0.57
    Marker Score: 548
  • Cell Name: squamous epithelial cell (CL0000076)
    Fold Change: 0.57
    Marker Score: 386
  • Cell Name: endothelial cell of hepatic sinusoid (CL1000398)
    Fold Change: 0.57
    Marker Score: 124
  • Cell Name: P/D1 enteroendocrine cell (CL0002268)
    Fold Change: 0.56
    Marker Score: 209
  • Cell Name: peritubular capillary endothelial cell (CL1001033)
    Fold Change: 0.56
    Marker Score: 130
  • Cell Name: late promyelocyte (CL0002151)
    Fold Change: 0.56
    Marker Score: 140
  • Cell Name: granulocyte monocyte progenitor cell (CL0000557)
    Fold Change: 0.56
    Marker Score: 354

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** 1. **Mediator Complex Subunit**: MED16 is a subunit of the Mediator complex, a protein complex that plays a central role in regulating gene expression by interacting with transcription factors and RNA polymerase II. 2. **Tissue-Specific Expression**: MED16 is significantly expressed in germ cells, oocytes, placental villous trophoblasts, and certain types of neurons, including GABAergic interneurons and forebrain radial glial cells. 3. **Protein-Protein Interactions**: MED16 interacts with various transcription factors and coactivators, including PPARα, PPARγ, and the Nuclear Thyroid Hormone Receptor (NTR). 4. **RNA Polymerase II Interaction**: MED16 interacts directly with RNA polymerase II, regulating transcription elongation and initiation. **Pathways and Functions:** 1. **Transcriptional Regulation**: MED16 plays a crucial role in regulating gene expression by interacting with transcription factors and RNA polymerase II, influencing the transcription of various genes involved in developmental biology, disease, and metabolism. 2. **Developmental Biology**: MED16 is essential for the development of germ cells, oocytes, and the placenta, highlighting its importance in embryonic development and reproductive biology. 3. **Metabolic Regulation**: MED16 interacts with PPARα and PPARγ, regulating lipid metabolism and energy homeostasis. 4. **Transcriptional Coactivation**: MED16 acts as a transcriptional coactivator, enhancing the expression of target genes involved in various biological processes. **Clinical Significance:** 1. **Cancer**: MED16 mutations have been implicated in certain types of cancer, including ovarian cancer and germ cell tumors. 2. **Neurological Disorders**: MED16 mutations have been associated with neurological disorders, such as autism spectrum disorder and schizophrenia. 3. **Metabolic Disorders**: MED16 interactions with PPARα and PPARγ suggest its potential role in regulating metabolic disorders, such as type 2 diabetes and obesity. 4. **Reproductive Biology**: MED16's importance in germ cell development and oocyte maturation highlights its potential role in reproductive health and fertility. In conclusion, MED16 is a multifunctional protein complex subunit that plays a critical role in regulating gene expression, developmental biology, and metabolic regulation. Its interactions with transcription factors and RNA polymerase II make it an essential component of the Mediator complex, influencing various biological processes and clinical disorders. Further research is necessary to fully elucidate the mechanisms of MED16 and its role in human disease.

Genular Protein ID: 948302423

Symbol: MED16_HUMAN

Name: Mediator of RNA polymerase II transcription subunit 16

UniProtKB Accession Codes:

Q9Y2X0 Q6PJT2 Q96AD4 Q96I35 Q9Y652

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CORUM 1 CTD 1 ChiTaRS 1 ComplexPortal 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 10 EMDB 3 EPD 1 Ensembl 3 ExpressionAtlas 1 GO 14 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 Genevisible 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 8 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MaxQB 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 8 PDBsum 7 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 4 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 5 Pumba 1 RNAct 1 Reactome 3 RefSeq 1 SAM 1 SIGNOR 1 SMART 1 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10198638

Title: Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.

PubMed ID: 10198638

DOI: 10.1016/s1097-2765(00)80463-3

PubMed ID: 10235266

Title: Ligand-dependent transcription activation by nuclear receptors requires the DRIP complex.

PubMed ID: 10235266

DOI: 10.1038/19783

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15175163

Title: A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.

PubMed ID: 15175163

DOI: 10.1016/j.molcel.2004.05.006

PubMed ID: 15989967

Title: MED1/TRAP220 exists predominantly in a TRAP/Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.

PubMed ID: 15989967

DOI: 10.1016/j.molcel.2005.05.015

Sequence Information:

  • Length: 877
  • Mass: 96793
  • Checksum: 15173F237A75D230
  • Sequence:
    • MCDLRRPAAG GMMDLAYVCE WEKWSKSTHC PSVPLACAWS CRNLIAFTMD LRSDDQDLTR 
MIHILDTEHP WDLHSIPSEH HEAITCLEWD QSGSRLLSAD ADGQIKCWSM ADHLANSWES 
SVGSLVEGDP IVALSWLHNG VKLALHVEKS GASSFGEKFS RVKFSPSLTL FGGKPMEGWI 
AVTVSGLVTV SLLKPSGQVL TSTESLCRLR GRVALADIAF TGGGNIVVAT ADGSSASPVQ 
FYKVCVSVVS EKCRIDTEIL PSLFMRCTTD LNRKDKFPAI THLKFLARDM SEQVLLCASS 
QTSSIVECWS LRKEGLPVNN IFQQISPVVG DKQPTILKWR ILSATNDLDR VSAVALPKLP 
ISLTNTDLKV ASDTQFYPGL GLALAFHDGS VHIVHRLSLQ TMAVFYSSAA PRPVDEPAMK 
RPRTAGPAVH LKAMQLSWTS LALVGIDSHG KLSVLRLSPS MGHPLEVGLA LRHLLFLLEY 
CMVTGYDWWD ILLHVQPSMV QSLVEKLHEE YTRQTAALQQ VLSTRILAMK ASLCKLSPCT 
VTRVCDYHTK LFLIAISSTL KSLLRPHFLN TPDKSPGDRL TEICTKITDV DIDKVMINLK 
TEEFVLDMNT LQALQQLLQW VGDFVLYLLA SLPNQGSLLR PGHSFLRDGT SLGMLRELMV 
VIRIWGLLKP SCLPVYTATS DTQDSMSLLF RLLTKLWICC RDEGPASEPD EALVDECCLL 
PSQLLIPSLD WLPASDGLVS RLQPKQPLRL QFGRAPTLPG SAATLQLDGL ARAPGQPKID 
HLRRLHLGAC PTEECKACTR CGCVTMLKSP NRTTAVKQWE QRWIKNCLAV EGRGPDACVT 
SRASEEAPAF VQLGPQSTHH SPRTPRSLDH LHPEDRP

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.