Details for: CHAF1A

Gene ID: 10036

Symbol: CHAF1A

Ensembl ID: ENSG00000167670

Description: chromatin assembly factor 1 subunit A

Associated with

Cells (max top 100)

(Marker Scores and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 1.51
    Marker Score: 2,175
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: 1.37
    Marker Score: 930
  • Cell Name: immature innate lymphoid cell (CL0001082)
    Fold Change: 1.18
    Marker Score: 2,407
  • Cell Name: cardiac endothelial cell (CL0010008)
    Fold Change: 1.08
    Marker Score: 2,053
  • Cell Name: migratory enteric neural crest cell (CL0002607)
    Fold Change: 1.06
    Marker Score: 993
  • Cell Name: large pre-B-II cell (CL0000957)
    Fold Change: 1.01
    Marker Score: 2,753
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71,778
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 48,003
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 0.98
    Marker Score: 15,389
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30,403
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.95
    Marker Score: 2,405
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.94
    Marker Score: 486
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 0.94
    Marker Score: 302
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.94
    Marker Score: 442
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.91
    Marker Score: 2,721
  • Cell Name: neural crest cell (CL0011012)
    Fold Change: 0.89
    Marker Score: 952
  • Cell Name: primitive red blood cell (CL0002355)
    Fold Change: 0.88
    Marker Score: 659
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.88
    Marker Score: 5,006
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.87
    Marker Score: 5,295
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.86
    Marker Score: 311
  • Cell Name: lymphoid lineage restricted progenitor cell (CL0000838)
    Fold Change: 0.81
    Marker Score: 487
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 0.79
    Marker Score: 995
  • Cell Name: fraction A pre-pro B cell (CL0002045)
    Fold Change: 0.79
    Marker Score: 791
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1,264
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 0.75
    Marker Score: 997
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.74
    Marker Score: 295
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.73
    Marker Score: 561
  • Cell Name: oocyte (CL0000023)
    Fold Change: 0.73
    Marker Score: 181
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.73
    Marker Score: 379
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 0.73
    Marker Score: 2,974
  • Cell Name: plasmacytoid dendritic cell, human (CL0001058)
    Fold Change: 0.7
    Marker Score: 183
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.69
    Marker Score: 1,179
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.69
    Marker Score: 2,050
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.68
    Marker Score: 625
  • Cell Name: kidney proximal straight tubule epithelial cell (CL1000839)
    Fold Change: 0.68
    Marker Score: 1,596
  • Cell Name: kidney capillary endothelial cell (CL1000892)
    Fold Change: 0.67
    Marker Score: 211
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.67
    Marker Score: 2,810
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.66
    Marker Score: 2,607
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.66
    Marker Score: 169
  • Cell Name: male germ cell (CL0000015)
    Fold Change: 0.62
    Marker Score: 178
  • Cell Name: Unknown (CL0000548)
    Fold Change: 0.61
    Marker Score: 446
  • Cell Name: neural progenitor cell (CL0011020)
    Fold Change: 0.61
    Marker Score: 2,350
  • Cell Name: late promyelocyte (CL0002151)
    Fold Change: 0.59
    Marker Score: 149
  • Cell Name: mesangial cell (CL0000650)
    Fold Change: 0.59
    Marker Score: 708
  • Cell Name: cerebellar granule cell precursor (CL0002362)
    Fold Change: 0.58
    Marker Score: 326
  • Cell Name: epithelial cell of nephron (CL1000449)
    Fold Change: 0.58
    Marker Score: 1,201
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.57
    Marker Score: 842
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: 0.57
    Marker Score: 236
  • Cell Name: smooth muscle myoblast (CL0000514)
    Fold Change: 0.56
    Marker Score: 265
  • Cell Name: intestinal enteroendocrine cell (CL1001516)
    Fold Change: 0.55
    Marker Score: 440
  • Cell Name: IgG plasmablast (CL0000982)
    Fold Change: 0.55
    Marker Score: 152
  • Cell Name: neuroblast (sensu Vertebrata) (CL0000031)
    Fold Change: 0.54
    Marker Score: 339
  • Cell Name: common myeloid progenitor (CL0000049)
    Fold Change: 0.53
    Marker Score: 140
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.52
    Marker Score: 333
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: 0.51
    Marker Score: 4,354
  • Cell Name: erythroblast (CL0000765)
    Fold Change: 0.48
    Marker Score: 297
  • Cell Name: taste receptor cell (CL0000209)
    Fold Change: 0.47
    Marker Score: 404
  • Cell Name: mesodermal cell (CL0000222)
    Fold Change: 0.47
    Marker Score: 6,158
  • Cell Name: promyelocyte (CL0000836)
    Fold Change: 0.47
    Marker Score: 230
  • Cell Name: enteric smooth muscle cell (CL0002504)
    Fold Change: 0.46
    Marker Score: 1,093
  • Cell Name: ovarian surface epithelial cell (CL2000064)
    Fold Change: 0.46
    Marker Score: 1,254
  • Cell Name: common lymphoid progenitor (CL0000051)
    Fold Change: 0.45
    Marker Score: 309
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.45
    Marker Score: 27,634
  • Cell Name: neuronal receptor cell (CL0000006)
    Fold Change: 0.45
    Marker Score: 198
  • Cell Name: erythroid progenitor cell (CL0000038)
    Fold Change: 0.44
    Marker Score: 464
  • Cell Name: osteoclast (CL0000092)
    Fold Change: 0.44
    Marker Score: 220
  • Cell Name: granulocyte monocyte progenitor cell (CL0000557)
    Fold Change: 0.44
    Marker Score: 280
  • Cell Name: precursor B cell (CL0000817)
    Fold Change: 0.43
    Marker Score: 284
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: 0.42
    Marker Score: 416
  • Cell Name: nasal mucosa goblet cell (CL0002480)
    Fold Change: 0.42
    Marker Score: 281
  • Cell Name: lamp5 GABAergic cortical interneuron (CL4023011)
    Fold Change: 0.42
    Marker Score: 6,238
  • Cell Name: stem cell (CL0000034)
    Fold Change: 0.42
    Marker Score: 986
  • Cell Name: pre-conventional dendritic cell (CL0002010)
    Fold Change: 0.41
    Marker Score: 122
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: 0.41
    Marker Score: 3,912
  • Cell Name: pulmonary interstitial fibroblast (CL0002241)
    Fold Change: 0.41
    Marker Score: 328
  • Cell Name: connective tissue cell (CL0002320)
    Fold Change: 0.41
    Marker Score: 106
  • Cell Name: neural cell (CL0002319)
    Fold Change: 0.41
    Marker Score: 196
  • Cell Name: exhausted T cell (CL0011025)
    Fold Change: 0.4
    Marker Score: 230
  • Cell Name: myeloid cell (CL0000763)
    Fold Change: 0.39
    Marker Score: 633
  • Cell Name: type EC enteroendocrine cell (CL0000577)
    Fold Change: 0.39
    Marker Score: 363
  • Cell Name: mature B cell (CL0000785)
    Fold Change: 0.38
    Marker Score: 281
  • Cell Name: kidney interstitial cell (CL1000500)
    Fold Change: 0.38
    Marker Score: 272
  • Cell Name: early lymphoid progenitor (CL0000936)
    Fold Change: 0.38
    Marker Score: 187
  • Cell Name: conventional dendritic cell (CL0000990)
    Fold Change: 0.38
    Marker Score: 259
  • Cell Name: extravillous trophoblast (CL0008036)
    Fold Change: 0.37
    Marker Score: 333
  • Cell Name: plasmacytoid dendritic cell (CL0000784)
    Fold Change: 0.37
    Marker Score: 200
  • Cell Name: choroid plexus epithelial cell (CL0000706)
    Fold Change: 0.35
    Marker Score: 324
  • Cell Name: thymocyte (CL0000893)
    Fold Change: 0.35
    Marker Score: 369
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.35
    Marker Score: 399
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: 0.35
    Marker Score: 543
  • Cell Name: dendritic cell (CL0000451)
    Fold Change: 0.35
    Marker Score: 241
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.35
    Marker Score: 161
  • Cell Name: megakaryocyte progenitor cell (CL0000553)
    Fold Change: 0.35
    Marker Score: 97
  • Cell Name: fetal cardiomyocyte (CL0002495)
    Fold Change: 0.35
    Marker Score: 114
  • Cell Name: megakaryocyte-erythroid progenitor cell (CL0000050)
    Fold Change: 0.34
    Marker Score: 144
  • Cell Name: glial cell (CL0000125)
    Fold Change: 0.34
    Marker Score: 378
  • Cell Name: fibroblast of cardiac tissue (CL0002548)
    Fold Change: 0.34
    Marker Score: 2,063
  • Cell Name: basal cell of epithelium of trachea (CL1000348)
    Fold Change: 0.34
    Marker Score: 2,548
  • Cell Name: sncg GABAergic cortical interneuron (CL4023015)
    Fold Change: 0.34
    Marker Score: 2,621
  • Cell Name: B cell (CL0000236)
    Fold Change: 0.34
    Marker Score: 325

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Marker Score to the Marker Score Threshold, indicating how much the gene expression has changed compared to a baseline.
Marker Score: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** The CHAF1A gene is a crucial component of the CAF1 complex, which is responsible for the assembly of nucleosomes during DNA replication and repair. The CAF1 complex consists of two subunits, p150 (CHAF1A) and p62 (CHAF1B), which interact with histones to facilitate nucleosome formation. The CHAF1A subunit is a large protein that contains a chromo shadow domain, which is essential for its interaction with histones and other chromatin-associated proteins. **Pathways and Functions:** The CHAF1A gene is involved in several key pathways, including: 1. **Chromatin Assembly:** CHAF1A plays a critical role in the assembly of nucleosomes during DNA replication and repair. The CAF1 complex interacts with histones to facilitate nucleosome formation, which is essential for the regulation of gene expression. 2. **Cell Cycle Regulation:** CHAF1A is involved in the regulation of the cell cycle, particularly during the G2/M phase. The CAF1 complex is required for the assembly of nucleosomes, which is necessary for the initiation of mitosis. 3. **DNA Repair:** CHAF1A is also involved in DNA repair mechanisms, particularly in the repair of double-strand breaks. The CAF1 complex interacts with histones and other chromatin-associated proteins to facilitate the repair of DNA damage. **Clinical Significance:** Dysregulation of the CHAF1A gene has been implicated in several diseases, including: 1. **Cancer:** Alterations in CHAF1A expression have been observed in various types of cancer, including breast, lung, and colon cancer. CHAF1A dysfunction can lead to chromosomal instability and increased tumorigenesis. 2. **Neurodegenerative Disorders:** CHAF1A has been implicated in neurodegenerative disorders, such as Alzheimer's disease and Huntington's disease. Dysregulation of CHAF1A expression has been observed in the brains of patients with these diseases, and CHAF1A dysfunction has been linked to chromatin instability and neuronal degeneration. 3. **Genetic Disorders:** CHAF1A mutations have been identified in individuals with genetic disorders, such as intellectual disability and developmental delay. In conclusion, the CHAF1A gene plays a crucial role in chromatin assembly, cell cycle regulation, and DNA repair. Dysregulation of CHAF1A expression has been implicated in several diseases, including cancer and neurodegenerative disorders. Further research is needed to fully understand the mechanisms by which CHAF1A regulates chromatin assembly and cell cycle progression, and to explore the therapeutic potential of CHAF1A-targeting interventions.

Genular Protein ID: 3926663281

Symbol: CAF1A_HUMAN

Name: Chromatin assembly factor I p150 subunit

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 11250073

Title: Genomic sequence and expression analyses of human chromatin assembly factor 1 p150 gene.

PubMed ID: 11250073

DOI: 10.1016/s0378-1119(01)00335-3

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 7600578

Title: The p150 and p60 subunits of chromatin assembly factor I: a molecular link between newly synthesized histones and DNA replication.

PubMed ID: 7600578

DOI: 10.1016/s0092-8674(05)80015-7

PubMed ID: 10052459

Title: Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin.

PubMed ID: 10052459

DOI: 10.1016/s0092-8674(00)80661-3

PubMed ID: 10648606

Title: A CAF-1-PCNA-mediated chromatin assembly pathway triggered by sensing DNA damage.

PubMed ID: 10648606

DOI: 10.1128/mcb.20.4.1206-1218.2000

PubMed ID: 10893180

Title: CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair.

PubMed ID: 10893180

DOI: 10.1242/jcs.113.15.2647

PubMed ID: 11296234

Title: Dimerization of the largest subunit of chromatin assembly factor 1: importance in vitro and during Xenopus early development.

PubMed ID: 11296234

DOI: 10.1093/emboj/20.8.2015

PubMed ID: 12697822

Title: The methyl-CpG binding protein MBD1 interacts with the p150 subunit of chromatin assembly factor 1.

PubMed ID: 12697822

DOI: 10.1128/mcb.23.9.3226-3236.2003

PubMed ID: 15327775

Title: Methyl-CpG binding protein MBD1 couples histone H3 methylation at lysine 9 by SETDB1 to DNA replication and chromatin assembly.

PubMed ID: 15327775

DOI: 10.1016/j.molcel.2004.06.043

PubMed ID: 30849389

Title: Retraction Notice to: Methyl-CpG Binding Protein MBD1 Couples Histone H3 Methylation at Lysine 9 by SETDB1 to DNA Replication and Chromatin Assembly.

PubMed ID: 30849389

DOI: 10.1016/j.molcel.2019.02.023

PubMed ID: 15882967

Title: The mammalian heterochromatin protein 1 binds diverse nuclear proteins through a common motif that targets the chromoshadow domain.

PubMed ID: 15882967

DOI: 10.1016/j.bbrc.2005.04.016

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20562864

Title: Human POGZ modulates dissociation of HP1alpha from mitotic chromosome arms through Aurora B activation.

PubMed ID: 20562864

DOI: 10.1038/ncb2075

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 25218447

Title: Uncovering global SUMOylation signaling networks in a site-specific manner.

PubMed ID: 25218447

DOI: 10.1038/nsmb.2890

PubMed ID: 25114211

Title: Mapping of SUMO sites and analysis of SUMOylation changes induced by external stimuli.

PubMed ID: 25114211

DOI: 10.1073/pnas.1413825111

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

PubMed ID: 33857403

Title: DNAJC9 integrates heat shock molecular chaperones into the histone chaperone network.

PubMed ID: 33857403

DOI: 10.1016/j.molcel.2021.03.041

Sequence Information:

  • Length: 956
  • Mass: 106910
  • Checksum: 90617F8FE8DB7FD0
  • Sequence:
  • MLEELECGAP GARGAATAMD CKDRPAFPVK KLIQARLPFK RLNLVPKGKA DDMSDDQGTS 
    VQSKSPDLEA SLDTLENNCH VGSDIDFRPK LVNGKGPLDN FLRNRIETSI GQSTVIIDLT 
    EDSNEQPDSL VDHNKLNSEA SPSREAINGQ REDTGDQQGL LKAIQNDKLA FPGETLSDIP 
    CKTEEEGVGC GGAGRRGDSQ ECSPRSCPEL TSGPRMCPRK EQDSWSEAGG ILFKGKVPMV 
    VLQDILAVRP PQIKSLPATP QGKNMTPESE VLESFPEEDS VLSHSSLSSP SSTSSPEGPP 
    APPKQHSSTS PFPTSTPLRR ITKKFVKGST EKNKLRLQRD QERLGKQLKL RAEREEKEKL 
    KEEAKRAKEE AKKKKEEEKE LKEKERREKR EKDEKEKAEK QRLKEERRKE RQEALEAKLE 
    EKRKKEEEKR LREEEKRIKA EKAEITRFFQ KPKTPQAPKT LAGSCGKFAP FEIKEHMVLA 
    PRRRTAFHPD LCSQLDQLLQ QQSGEFSFLK DLKGRQPLRS GPTHVSTRNA DIFNSDVVIV 
    ERGKGDGVPE RRKFGRMKLL QFCENHRPAY WGTWNKKTAL IRARDPWAQD TKLLDYEVDS 
    DEEWEEEEPG ESLSHSEGDD DDDMGEDEDE DDGFFVPHGY LSEDEGVTEE CADPENHKVR 
    QKLKAKEWDE FLAKGKRFRV LQPVKIGCVW AADRDCAGDD LKVLQQFAAC FLETLPAQEE 
    QTPKASKRER RDEQILAQLL PLLHGNVNGS KVIIREFQEH CRRGLLSNHT GSPRSPSTTY 
    LHTPTPSEDA AIPSKSRLKR LISENSVYEK RPDFRMCWYV HPQVLQSFQQ EHLPVPCQWS 
    YVTSVPSAPK EDSGSVPSTG PSQGTPISLK RKSAGSMCIT QFMKKRRHDG QIGAEDMDGF 
    QADTEEEEEE EGDCMIVDVP DAAEVQAPCG AASGAGGGVG VDTGKATLTA SPLGAS

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.