Details for: KIF20A

Gene ID: 10112

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: KIF20A

Ensembl ID: ENSG00000112984

Description: kinesin family member 20A

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • neural crest cell CL0011012
    CSI 3.87
    rCSI 3.06%
    PRS 98.81
  • pro-B cell CL0000826
    CSI 3.62
    rCSI 3%
    PRS 99.37
  • mesodermal cell CL0000222
    CSI 3.33
    rCSI 4%
    PRS 99.52
  • large pre-B-II cell CL0000957
    CSI 2.71
    rCSI 7.73%
    PRS 98.88
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.56
    rCSI 2.96%
    PRS 97.8
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.45
    rCSI 3.15%
    PRS 98.5
  • vascular associated smooth muscle cell CL0000359
    CSI 2.31
    rCSI 7.48%
    PRS 99.27
  • promonocyte CL0000559
    CSI 1.73
    rCSI 2.96%
    PRS 99.56
  • stromal cell CL0000499
    CSI 1.7
    rCSI 4.78%
    PRS 98.65
  • mesenchymal cell CL0008019
    CSI 1.11
    rCSI 2.82%
    PRS 98.9
  • primitive red blood cell CL0002355
    CSI 0.95
    rCSI 5.11%
    PRS 99.16

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [KIF20A](/details-gene/10112) is a protein-coding gene located on chromosome 5q31.2 that encodes Kinesin Family Member 20A. This protein is a microtubule-dependent motor protein crucial for cellular division, particularly during the final stage of cytokinesis. Functional annotations strongly link [KIF20A](/details-gene/10112) to processes such as [mitotic cytokinesis](/details-cell/GO:0000281), [microtubule-based movement](/details-cell/GO:0007018), and the formation of the midbody structure that separates daughter cells. Its expression profile indicates high significance in various progenitor and rapidly proliferating cell populations, including [neural crest cells](/details-cell/CL0011012), [pro-B cells](/details-cell/CL0000826), and [mesodermal cells](/details-cell/CL0000222), highlighting its fundamental role in development and tissue renewal. Loss-of-function mutations in [KIF20A](/details-gene/10112) have been associated with severe human pathology, including lethal congenital cardiomyopathy ([Link](https://doi.org/10.1371/journal.pgen.1007138)). ## Cellular Roles and Expression Landscape The expression pattern of [KIF20A](/details-gene/10112) underscores its essential function in cell proliferation across multiple lineages. **Overall**, the gene shows the highest significance in progenitor and precursor cell types, suggesting it is a key component of the machinery required for rapid cell division during development and hematopoiesis. Its prominence in developmental cell types is marked by high significance scores in [neural crest cell](/details-cell/CL0011012) (CSI: 3.87), [neuroblast (sensu Vertebrata)](/details-cell/CL0000031) (CSI: 2.45), and [mesodermal cell](/details-cell/CL0000222) (CSI: 3.33). This is consistent with a critical role in the expansion of these foundational cell populations during embryogenesis. Within the hematopoietic system, [KIF20A](/details-gene/10112) is a significant marker for early lymphoid and myeloid progenitors. It is highly ranked in [pro-B cell](/details-cell/CL0000826) (CSI: 3.62), [large pre-B-II cell](/details-cell/CL0000957) (CSI: 2.71), and [promonocyte](/details-cell/CL0000559) (CSI: 1.73). This expression profile suggests its activity is essential for the proliferative bursts that characterize the development of immune cells. The gene's role is not limited to specific lineages, as evidenced by its significance in [mesenchymal cell](/details-cell/CL0008019) (CSI: 1.11) and [vascular associated smooth muscle cell](/details-cell/CL0000359) (CSI: 2.31), indicating a broad requirement in proliferating stromal and structural cells. ## Pathways and Molecular Function The molecular functions of [KIF20A](/details-gene/10112) are tightly centered on its identity as a kinesin motor protein. Gene Ontology annotations confirm its involvement in [ATP binding](/details-cell/GO:0005524) and [microtubule motor activity](/details-cell/GO:0003777), which are fundamental to its role in intracellular transport and force generation. It is an integral component of the [kinesin complex](/details-cell/GO:0005871) and localizes to key structures involved in cell division, including the [spindle](/details-cell/GO:0005819), [intercellular bridge](/details-cell/GO:0045171), and [midbody](/details-cell/GO:0030496). Consistent with these molecular roles, [KIF20A](/details-gene/10112) is a key player in the [Cell cycle](/details-cell/R-HSA-1640170) pathway, specifically during [M phase](/details-cell/R-HSA-68886) and [Mitotic Telophase/Cytokinesis](/details-cell/R-HSA-68884). Research has shown that its function in cytokinesis is regulated by phosphorylation through Polo-like kinase 1, which is required for the final separation of daughter cells ([Link](https://doi.org/10.1083/jcb.200306009)). Beyond cell division, its annotation in pathways like [Vesicle-mediated transport](/details-cell/R-HSA-5653656) and [Golgi-to-ER retrograde transport](/details-cell/R-HSA-8856688) suggests a broader role in intracellular trafficking. This is further supported by its involvement in the [MHC class II antigen presentation](/details-cell/R-HSA-2132295) pathway, hinting at a potential role in transporting vesicles within immune cells, which aligns with its expression in hematopoietic progenitors. ## Research Directions The established role of [KIF20A](/details-gene/10112) in cell division, combined with its specific expression pattern and links to disease, opens several avenues for future investigation. **Proposed Hypotheses:** 1. Given its high expression in progenitor cells like [neuroblasts](/details-cell/CL0000031) and its function in orienting the mitotic spindle, [KIF20A](/details-gene/10112) may be a critical determinant of symmetric versus asymmetric cell division, thereby influencing cell fate decisions and stem cell pool maintenance during neurogenesis. 2. The association of [KIF20A](/details-gene/10112) with the [MHC class II antigen presentation](/details-cell/R-HSA-2132295) pathway suggests a non-mitotic function in professional antigen-presenting cells. It may mediate the transport of peptide-loaded MHC-II vesicles from endosomal compartments to the cell surface, thereby directly impacting the initiation of adaptive immune responses. 3. Based on the finding that biallelic loss-of-function mutations cause lethal congenital cardiomyopathy ([Link](https://doi.org/10.1371/journal.pgen.1007138)), we hypothesize that [KIF20A](/details-gene/10112) is essential for the proper cytokinesis of cardiomyocytes during heart development. Its absence likely leads to failed cell division, resulting in multinucleated and functionally impaired cardiomyocytes that cannot form a viable myocardium. **Experimental Approach:** To test the third hypothesis regarding cardiomyopathy, an ideal approach would involve using human induced pluripotent stem cells (iPSCs) with CRISPR-Cas9-mediated knockout of [KIF20A](/details-gene/10112). These knockout iPSCs and isogenic controls could be differentiated into cardiomyocytes. The resulting cultures would be analyzed using high-resolution microscopy and live-cell imaging to assess for cytokinesis failure, multinucleation, and defects in sarcomere assembly. Functional assays, such as contractility measurements on micro-engineered platforms, would further determine the impact of [KIF20A](/details-gene/10112) loss on cardiomyocyte function. **Therapeutic Potential:** As a crucial mitotic protein, [KIF20A](/details-gene/10112) represents a compelling therapeutic target for hyperproliferative disorders, particularly cancer. Its high expression in rapidly dividing cells suggests that its **inhibition** could be an effective strategy to arrest tumor growth. Cancers originating from hematopoietic or neural progenitor cells, where [KIF20A](/details-gene/10112) expression is naturally high, may be particularly vulnerable. The development of small molecule inhibitors targeting its motor domain's ATPase activity could offer a targeted antimitotic therapy with a potentially favorable therapeutic window, assuming lower dependence in terminally differentiated, non-dividing healthy tissues.

Genular Protein ID: 602725466

Symbol: KI20A_HUMAN

Name: Kinesin-like protein KIF20A

UniProtKB Accession Codes:

O95235 B4DL79 D3DQB6

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 2 CTD 1 ChEBI 1 ChEMBL 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 8 Ensembl 2 ExpressionAtlas 1 GO 19 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 3 MINT 1 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 1 PDBsum 1 PRINTS 1 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 Reactome 4 RefSeq 1 SIGNOR 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10233894

Title: Vascular endothelial genes that are responsive to tumor necrosis factor-alpha in vitro are expressed in atherosclerotic lesions, including inhibitor of apoptosis protein-1, stannin, and two novel genes.

PubMed ID: 10233894

PubMed ID: 10806357

Title: cDNA cloning, expression pattern, genomic structure and chromosomal location of RAB6KIFL, a human kinesin-like gene.

PubMed ID: 10806357

DOI: 10.1016/s0378-1119(00)00135-9

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15372022

Title: The DNA sequence and comparative analysis of human chromosome 5.

PubMed ID: 15372022

DOI: 10.1038/nature02919

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 12939256

Title: Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis.

PubMed ID: 12939256

DOI: 10.1083/jcb.200306009

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18691976

Title: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

PubMed ID: 18691976

DOI: 10.1016/j.molcel.2008.07.007

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 29357359

Title: Compound heterozygous loss-of-function mutations in KIF20A are associated with a novel lethal congenital cardiomyopathy in two siblings.

PubMed ID: 29357359

DOI: 10.1371/journal.pgen.1007138

Sequence Information:

  • Length: 890
  • Mass: 100278
  • Checksum: 6620264615496051
  • Sequence:
    • MSQGILSPPA GLLSDDDVVV SPMFESTAAD LGSVVRKNLL SDCSVVSTSL EDKQQVPSED 
SMEKVKVYLR VRPLLPSELE RQEDQGCVRI ENVETLVLQA PKDSFALKSN ERGIGQATHR 
FTFSQIFGPE VGQASFFNLT VKEMVKDVLK GQNWLIYTYG VTNSGKTHTI QGTIKDGGIL 
PRSLALIFNS LQGQLHPTPD LKPLLSNEVI WLDSKQIRQE EMKKLSLLNG GLQEEELSTS 
LKRSVYIESR IGTSTSFDSG IAGLSSISQC TSSSQLDETS HRWAQPDTAP LPVPANIRFS 
IWISFFEIYN ELLYDLLEPP SQQRKRQTLR LCEDQNGNPY VKDLNWIHVQ DAEEAWKLLK 
VGRKNQSFAS THLNQNSSRS HSIFSIRILH LQGEGDIVPK ISELSLCDLA GSERCKDQKS 
GERLKEAGNI NTSLHTLGRC IAALRQNQQN RSKQNLVPFR DSKLTRVFQG FFTGRGRSCM 
IVNVNPCAST YDETLHVAKF SAIASQLVHA PPMQLGFPSL HSFIKEHSLQ VSPSLEKGAK 
ADTGLDDDIE NEADISMYGK EELLQVVEAM KTLLLKERQE KLQLEMHLRD EICNEMVEQM 
QQREQWCSEH LDTQKELLEE MYEEKLNILK ESLTSFYQEE IQERDEKIEE LEALLQEARQ 
QSVAHQQSGS ELALRRSQRL AASASTQQLQ EVKAKLQQCK AELNSTTEEL HKYQKMLEPP 
PSAKPFTIDV DKKLEEGQKN IRLLRTELQK LGESLQSAER ACCHSTGAGK LRQALTTCDD 
ILIKQDQTLA ELQNNMVLVK LDLRKKAACI AEQYHTVLKL QGQVSAKKRL GTNQENQQPN 
QQPPGKKPFL RNLLPRTPTC QSSTDCSPYA RILRSRRSPL LKSGPFGKKY