Genular: Defining Cellular Identity and Function

Details for: Dhfr

Gene ID: 13361

Symbol: Dhfr

Ensembl ID: ENSMUSG00000021707

Description: dihydrofolate reductase

Associated with

Metabolism
(R-MMU-1430728)
Metabolism of folate and pterines
(R-MMU-196757)
Metabolism of vitamins and cofactors
(R-MMU-196854)
Metabolism of water-soluble vitamins and cofactors
(R-MMU-196849)
Axon regeneration
(GO:0031103)
Cytoplasm
(GO:0005737)
Cytosol
(GO:0005829)
Dihydrofolate metabolic process
(GO:0046452)
Dihydrofolate reductase activity
(GO:0004146)
Dihydrofolic acid binding
(GO:0051871)
Folic acid binding
(GO:0005542)
Folic acid metabolic process
(GO:0046655)
Glycine biosynthetic process
(GO:0006545)
Mitochondrial inner membrane
(GO:0005743)
Mitochondrial matrix
(GO:0005759)
Mitochondrion
(GO:0005739)
Mrna binding
(GO:0003729)
Mrna regulatory element binding translation repressor activity
(GO:0000900)
Nadp binding
(GO:0050661)
Nadph binding
(GO:0070402)
Nucleus
(GO:0005634)
One-carbon metabolic process
(GO:0006730)
Oxidoreductase activity
(GO:0016491)
Positive regulation of nitric-oxide synthase activity
(GO:0051000)
Regulation of removal of superoxide radicals
(GO:2000121)
Response to methotrexate
(GO:0031427)
Rna binding
(GO:0003723)
Sequence-specific mrna binding
(GO:1990825)
Tetrahydrobiopterin biosynthetic process
(GO:0006729)
Tetrahydrofolate biosynthetic process
(GO:0046654)
Tetrahydrofolate interconversion
(GO:0035999)

Other Information

Proteins

Dihydrofolate reductase

Genular Protein ID: 1505106490

Symbol: DYR_MOUSE

Name: Dihydrofolate reductase

UniProtKB Accession Codes:

P00375 P70693 Q61485 Q61487 Q61579

Database IDs:

Citations:

PubMed ID: 6282858

Title: Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells.

PubMed ID: 6282858

DOI: 10.1016/s0021-9258(18)34465-x

PubMed ID: 6573667

Title: Isolation and expression of an altered mouse dihydrofolate reductase cDNA.

PubMed ID: 6573667

DOI: 10.1073/pnas.80.9.2495

PubMed ID: 2263462

Title: Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells.

PubMed ID: 2263462

DOI: 10.1093/nar/18.23.7025

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 762074

Title: The amino acid sequence of dihydrofolate reductase from the mouse lymphoma L1210.

PubMed ID: 762074

DOI: 10.1016/s0021-9258(17)37942-5

PubMed ID: 2982814

Title: Heterogeneity at the 5' termini of mouse dihydrofolate reductase mRNAs. Evidence for multiple promoter regions.

PubMed ID: 2982814

DOI: 10.1016/s0021-9258(18)89555-2

PubMed ID: 6244105

Title: Structure and genomic organization of the mouse dihydrofolate reductase gene.

PubMed ID: 6244105

DOI: 10.1016/0092-8674(80)90510-3

PubMed ID: 360074

Title: Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase.

PubMed ID: 360074

DOI: 10.1038/275617a0

PubMed ID: 6121807

Title: Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene.

PubMed ID: 6121807

DOI: 10.1016/s0021-9258(18)34647-7

PubMed ID: 21183079

Title: A tissue-specific atlas of mouse protein phosphorylation and expression.

PubMed ID: 21183079

DOI: 10.1016/j.cell.2010.12.001

PubMed ID: 23806337

Title: SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways.

PubMed ID: 23806337

DOI: 10.1016/j.molcel.2013.06.001

PubMed ID: 25980602

Title: An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria.

PubMed ID: 25980602

DOI: 10.1016/j.febslet.2015.05.017

PubMed ID: 15681865

Title: Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH.

PubMed ID: 15681865

DOI: 10.1107/s0907444904030422

PubMed ID: 17019704

Title: New insights into DHFR interactions: analysis of Pneumocystis carinii and mouse DHFR complexes with NADPH and two highly potent 5-(omega-carboxy(alkyloxy) trimethoprim derivatives reveals conformational correlations with activity and novel parallel ring stacking interactions.

PubMed ID: 17019704

DOI: 10.1002/prot.21131

PubMed ID: 18703847

Title: Structural analysis of a holoenzyme complex of mouse dihydrofolate reductase with NADPH and a ternary complex with the potent and selective inhibitor 2,4-diamino-6-(2'-hydroxydibenz[b,f]azepin-5-yl)methylpteridine.

PubMed ID: 18703847

DOI: 10.1107/s0907444908022348

PubMed ID: 19748785

Title: Design, synthesis, and X-ray crystal structures of 2,4-diaminofuro[2,3-d]pyrimidines as multireceptor tyrosine kinase and dihydrofolate reductase inhibitors.

PubMed ID: 19748785

DOI: 10.1016/j.bmc.2009.08.044

Sequence Information:

Length: 187
Mass: 21606
Checksum: 47AEF15F879B119C
Sequence:

MVRPLNCIVA VSQNMGIGKN GDLPWPPLRN EFKYFQRMTT TSSVEGKQNL VIMGRKTWFS 
IPEKNRPLKD RINIVLSREL KEPPRGAHFL AKSLDDALRL IEQPELASKV DMVWIVGGSS 
VYQEAMNQPG HLRLFVTRIM QEFESDTFFP EIDLGKYKLL PEYPGVLSEV QEEKGIKYKF 
EVYEKKD

The gene details are incomplete or unavailable at the moment.

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.

Details for: Dhfr

Associated with

Other Information

Structure of amplified normal and variant dihydrofolate reductase genes in mouse sarcoma S180 cells. PubMed ID: 6282858

Isolation and expression of an altered mouse dihydrofolate reductase cDNA. PubMed ID: 6573667

Isolation and characterization of a variant dihydrofolate reductase cDNA from methotrexate-resistant murine L5178Y cells. PubMed ID: 2263462

The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). PubMed ID: 15489334

The amino acid sequence of dihydrofolate reductase from the mouse lymphoma L1210. PubMed ID: 762074

Heterogeneity at the 5' termini of mouse dihydrofolate reductase mRNAs. Evidence for multiple promoter regions. PubMed ID: 2982814

Structure and genomic organization of the mouse dihydrofolate reductase gene. PubMed ID: 6244105

Phenotypic expression in E. coli of a DNA sequence coding for mouse dihydrofolate reductase. PubMed ID: 360074

Nucleotide sequence surrounding multiple polyadenylation sites in the mouse dihydrofolate reductase gene. PubMed ID: 6121807

A tissue-specific atlas of mouse protein phosphorylation and expression. PubMed ID: 21183079

SIRT5-mediated lysine desuccinylation impacts diverse metabolic pathways. PubMed ID: 23806337

An active second dihydrofolate reductase enzyme is not a feature of rat and mouse, but they do have activity in their mitochondria. PubMed ID: 25980602

Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH. PubMed ID: 15681865

New insights into DHFR interactions: analysis of Pneumocystis carinii and mouse DHFR complexes with NADPH and two highly potent 5-(omega-carboxy(alkyloxy) trimethoprim derivatives reveals conformational correlations with activity and novel parallel ring stacking interactions. PubMed ID: 17019704

Structural analysis of a holoenzyme complex of mouse dihydrofolate reductase with NADPH and a ternary complex with the potent and selective inhibitor 2,4-diamino-6-(2'-hydroxydibenz[b,f]azepin-5-yl)methylpteridine. PubMed ID: 18703847

Design, synthesis, and X-ray crystal structures of 2,4-diaminofuro[2,3-d]pyrimidines as multireceptor tyrosine kinase and dihydrofolate reductase inhibitors. PubMed ID: 19748785