Details for: CYP2F1

Gene ID: 1572

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CYP2F1

Ensembl ID: ENSG00000197446

Description: cytochrome P450 family 2 subfamily F member 1

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • secretory cell CL0000151
    CSI 22.36
    rCSI 23.33%
    PRS 96.71
  • tracheal goblet cell CL1000329
    CSI 19.76
    rCSI 43.13%
    PRS 97.55
  • goblet cell CL0000160
    CSI 17.89
    rCSI 16.91%
    PRS 96.04
  • duct epithelial cell CL0000068
    CSI 16.4
    rCSI 23.99%
    PRS 98.44
  • nasal mucosa goblet cell CL0002480
    CSI 14.52
    rCSI 16.84%
    PRS 96.56
  • epithelial cell of lower respiratory tract CL0002632
    CSI 13.63
    rCSI 10.57%
    PRS 98.39
  • lung secretory cell CL1000272
    CSI 11.84
    rCSI 29.32%
    PRS 98.1
  • multi-ciliated epithelial cell CL0005012
    CSI 10.96
    rCSI 10.94%
    PRS 94.22
  • bronchial goblet cell CL1000312
    CSI 7.62
    rCSI 30.47%
    PRS 98.45
  • squamous epithelial cell CL0000076
    CSI 7.13
    rCSI 16.93%
    PRS 94.02
  • pulmonary ionocyte CL0017000
    CSI 6.33
    rCSI 7.7%
    PRS 98.05
  • respiratory basal cell CL0002633
    CSI 5.76
    rCSI 5.97%
    PRS 97.71
  • club cell CL0000158
    CSI 5.68
    rCSI 8.32%
    PRS 95.78
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 5.17
    rCSI 11.8%
    PRS 92.7
  • acinar cell CL0000622
    CSI 4.98
    rCSI 7.31%
    PRS 98.59
  • ionocyte CL0005006
    CSI 4.64
    rCSI 4.97%
    PRS 97.59
  • respiratory goblet cell CL0002370
    CSI 4.58
    rCSI 49.86%
    PRS 98.25
  • airway submucosal gland duct basal cell CL4033024
    CSI 2.45
    rCSI 15.68%
    PRS 97.9
  • respiratory suprabasal cell CL4033048
    CSI 2.43
    rCSI 3.11%
    PRS 97.78
  • tracheobronchial serous cell CL0019001
    CSI 2.31
    rCSI 10%
    PRS 97.78
  • basal cell of epithelium of trachea CL1000348
    CSI 2.03
    rCSI 14.34%
    PRS 97.04

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CYP2F1](/details-gene/1572) (cytochrome P450 family 2 subfamily F member 1) is a protein-coding gene located on chromosome 19q13.2. As a member of the extensive cytochrome P450 superfamily of monooxygenases, [CYP2F1](/details-gene/1572) is primarily involved in the metabolism of xenobiotics and endogenous compounds. Expression data highlights its highly specific and abundant role within the respiratory system, with significant expression in various secretory and epithelial cell types, including [secretory cell](/details-cell/CL0000151) and [tracheal goblet cell](/details-cell/CL1000329). Its function is crucial for processing inhaled substances, and it is clinically associated with OMIM entry [153245](https://omim.org/entry/124070). ## Cellular Roles and Expression Landscape The expression profile of [CYP2F1](/details-gene/1572) firmly establishes it as a key enzyme in the epithelial lining of the respiratory and secretory tracts. **Overall**, the gene shows the highest significance in cell types responsible for producing and secreting substances at mucosal surfaces. Its most prominent expression is observed in [secretory cell](/details-cell/CL0000151) (CSI: 22.36), [tracheal goblet cell](/details-cell/CL1000329) (CSI: 19.76), and [goblet cell](/details-cell/CL0000160) (CSI: 17.89). This pattern extends throughout the respiratory system, including high significance in [nasal mucosa goblet cell](/details-cell/CL0002480), [epithelial cell of lower respiratory tract](/details-cell/CL0002632), [lung secretory cell](/details-cell/CL1000272), and [club cell](/details-cell/CL0000158). This localized expression pattern suggests a specialized function as a first-pass metabolic barrier against inhaled compounds. Its presence in cells like [duct epithelial cell](/details-cell/CL0000068) and [acinar cell](/details-cell/CL0000622) is also consistent with a role in metabolizing substances within glandular tissues. The collective data points to [CYP2F1](/details-gene/1572) as a sentinel enzyme, critical for the detoxification and processing of foreign chemicals at their primary point of entry into the body via the airways. ## Pathways and Molecular Function The functional annotations for [CYP2F1](/details-gene/1572) align precisely with its role as a cytochrome P450 enzyme. It is an integral component of the [Endoplasmic reticulum membrane](/details-ontology/GO:0005789) where it exerts its catalytic functions. Molecularly, it exhibits [Monooxygenase activity](/details-ontology/GO:0004497), [Heme binding](/details-ontology/GO:0020037), and [Iron ion binding](/details-ontology/GO:0005506), which are characteristic of P450 enzymes. [CYP2F1](/details-gene/1572) participates in broad biological processes such as [Xenobiotic metabolic process](/details-ontology/GO:0006805) and [Response to toxic substance](/details-ontology/GO:0009636), as detailed in the Reactome pathways [Xenobiotics](/details-pathway/R-HSA-211981) and [Phase i - functionalization of compounds](/details-pathway/R-HSA-211945). Research has demonstrated its ability to metabolize specific substrates, including the epoxidation of naphthalene and the dehydrogenation of 3-methylindole, which are known pulmonary toxicants [Link](https://pubmed.ncbi.nlm.nih.gov/10383923). Furthermore, its annotated [Arachidonate epoxygenase activity](/details-ontology/GO:0008392) suggests a potential role in endogenous signaling pathways by metabolizing fatty acids into bioactive lipid mediators, which could influence inflammation and cellular homeostasis in the lung. ## Research Directions The highly localized expression of [CYP2F1](/details-gene/1572) in the respiratory tract makes it a critical gene for understanding individual susceptibility to lung diseases caused by environmental or occupational exposures. **Proposed Hypotheses:** 1. Genetic polymorphisms in [CYP2F1](/details-gene/1572), particularly non-functional variants [Link](https://doi.org/10.1016/j.mrfmmm.2007.01.007), may significantly increase an individual's risk of developing lung injury or cancer following exposure to specific inhaled pro-toxicants (e.g., naphthalene from tobacco smoke or industrial emissions) that require metabolic activation by this enzyme. 2. Beyond detoxification, [CYP2F1](/details-gene/1572) may modulate airway inflammation in diseases like asthma or chronic obstructive pulmonary disease (COPD) by regulating the local balance of pro- and anti-inflammatory lipid mediators derived from its [Arachidonate epoxygenase activity](/details-ontology/GO:0008392). **Experimental Approach:** To test the first hypothesis, a robust experimental design would involve using human bronchial epithelial cells grown at an air-liquid interface to model the airway. Cells from donors with different [CYP2F1](/details-gene/1572) genotypes (e.g., wild-type vs. the non-functional variant) could be exposed to controlled doses of a known substrate like naphthalene. Subsequent analysis of cell viability, DNA adduct formation, and markers of oxidative stress would directly assess whether the functional absence of [CYP2F1](/details-gene/1572) is protective or sensitizing, clarifying its role in either detoxification or toxicant activation. **Therapeutic Potential:** [CYP2F1](/details-gene/1572) is not a conventional therapeutic target for inhibition or activation in the context of disease treatment. Instead, its primary clinical relevance lies in pharmacogenomics and toxicology. Understanding an individual's [CYP2F1](/details-gene/1572) activity could be crucial for predicting their response to inhaled drugs that are metabolized by this enzyme or for assessing their susceptibility to lung damage from environmental pollutants. Therefore, it represents a key biomarker for personalized risk assessment and preventative strategies rather than a direct target for therapeutic intervention. Inhibition could be considered in specific scenarios to prevent the metabolic activation of certain pro-carcinogens within the lung.

Genular Protein ID: 1847476192

Symbol: CP2F1_HUMAN

Name: Cytochrome P450 2F1

UniProtKB Accession Codes:

P24903 A7KAU6 A7KAU7 A7KAU8 A7KAU9 A7KAV0 Q32MN5 Q8WWJ2

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BindingDB 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChEBI 10 ChEMBL 1 DMDM 1 DNASU 1 DisGeNET 1 EC 1 EMBL 12 Ensembl 2 ExpressionAtlas 1 GO 14 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PIR 2 PRINTS 3 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 Reactome 3 RefSeq 1 Rhea 3 SABIO-RK 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 1974816

Title: The human CYP2F gene subfamily: identification of a cDNA encoding a new cytochrome P450, cDNA-directed expression, and chromosome mapping.

PubMed ID: 1974816

DOI: 10.1021/bi00475a012

PubMed ID: 11827709

Title: Identification and cross-species comparisons of CYP2F subfamily genes in mammals.

PubMed ID: 11827709

DOI: 10.1016/s0027-5107(01)00274-3

PubMed ID: 17327131

Title: Molecular analysis of the CYP2F1 gene: identification of a frequent non-functional allelic variant.

PubMed ID: 17327131

DOI: 10.1016/j.mrfmmm.2007.01.007

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 8619884

Title: Expression of xenobiotic-metabolizing cytochrome P450 forms in human full-term placenta.

PubMed ID: 8619884

DOI: 10.1016/0006-2952(95)02184-1

PubMed ID: 8558432

Title: Metabolism of 3-methylindole by vaccinia-expressed P450 enzymes: correlation of 3-methyleneindolenine formation and protein-binding.

PubMed ID: 8558432

PubMed ID: 10383923

Title: Specific dehydrogenation of 3-methylindole and epoxidation of naphthalene by recombinant human CYP2F1 expressed in lymphoblastoid cells.

PubMed ID: 10383923

Sequence Information:

  • Length: 491
  • Mass: 55501
  • Checksum: D8022271FC0B6D06
  • Sequence:
    • MDSISTAILL LLLALVCLLL TLSSRDKGKL PPGPRPLSIL GNLLLLCSQD MLTSLTKLSK 
EYGSMYTVHL GPRRVVVLSG YQAVKEALVD QGEEFSGRGD YPAFFNFTKG NGIAFSSGDR 
WKVLRQFSIQ ILRNFGMGKR SIEERILEEG SFLLAELRKT EGEPFDPTFV LSRSVSNIIC 
SVLFGSRFDY DDERLLTIIR LINDNFQIMS SPWGELYDIF PSLLDWVPGP HQRIFQNFKC 
LRDLIAHSVH DHQASLDPRS PRDFIQCFLT KMAEEKEDPL SHFHMDTLLM TTHNLLFGGT 
KTVSTTLHHA FLALMKYPKV QARVQEEIDL VVGRARLPAL KDRAAMPYTD AVIHEVQRFA 
DIIPMNLPHR VTRDTAFRGF LIPKGTDVIT LLNTVHYDPS QFLTPQEFNP EHFLDANQSF 
KKSPAFMPFS AGRRLCLGES LARMELFLYL TAILQSFSLQ PLGAPEDIDL TPLSSGLGNL 
PRPFQLCLRP R