Details for: CYB5R3

Gene ID: 1727

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CYB5R3

Ensembl ID: ENSG00000100243

Description: cytochrome b5 reductase 3

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • skin fibroblast CL0002620
    CSI 32.16
    rCSI 27.72%
    PRS 29.93
  • pancreatic A cell CL0000171
    CSI 28.01
    rCSI 29.35%
    PRS 20.41
  • bronchus fibroblast of lung CL2000093
    CSI 23.78
    rCSI 19.32%
    PRS 20.23
  • microcirculation associated smooth muscle cell CL0008035
    CSI 23.63
    rCSI 68.41%
    PRS 21.64
  • interstitial cell of Cajal CL0002088
    CSI 21.63
    rCSI 27.53%
    PRS 22.23
  • intestinal epithelial cell CL0002563
    CSI 21.36
    rCSI 22.33%
    PRS 19.98
  • pancreatic ductal cell CL0002079
    CSI 19.16
    rCSI 37.27%
    PRS 19.59
  • keratocyte CL0002363
    CSI 18.38
    rCSI 44.17%
    PRS 28.26
  • retinal blood vessel endothelial cell CL0002585
    CSI 17.95
    rCSI 28.66%
    PRS 21.02
  • peripheral nervous system neuron CL2000032
    CSI 17.64
    rCSI 24.03%
    PRS 16.95
  • endothelial cell of artery CL1000413
    CSI 17.19
    rCSI 25.19%
    PRS 67.86
  • endothelial cell of lymphatic vessel CL0002138
    CSI 15.45
    rCSI 30.61%
    PRS 51.03
  • intrahepatic cholangiocyte CL0002538
    CSI 14.34
    rCSI 34.4%
    PRS 34
  • conjunctival epithelial cell CL1000432
    CSI 13.74
    rCSI 20.98%
    PRS 19.28
  • pancreatic PP cell CL0002275
    CSI 11.94
    rCSI 47.54%
    PRS 32.8
  • myofibroblast cell CL0000186
    CSI 11.81
    rCSI 16.35%
    PRS 27.06
  • megakaryocyte CL0000556
    CSI 11.56
    rCSI 50.16%
    PRS 33.28
  • conventional dendritic cell CL0000990
    CSI 11.2
    rCSI 9.35%
    PRS 49.18
  • duct epithelial cell CL0000068
    CSI 11.15
    rCSI 16.31%
    PRS 20.28
  • pancreatic stellate cell CL0002410
    CSI 10.76
    rCSI 62.58%
    PRS 28.43
  • type EC enteroendocrine cell CL0000577
    CSI 10.57
    rCSI 37.52%
    PRS 31.05
  • pancreatic epsilon cell CL0005019
    CSI 10.1
    rCSI 47.09%
    PRS 42.45
  • perivascular cell CL4033054
    CSI 9.94
    rCSI 13.59%
    PRS 21.68
  • vein endothelial cell of respiratory system CL4033008
    CSI 9.89
    rCSI 67.93%
    PRS 36.94
  • stromal cell of ovary CL0002132
    CSI 9.76
    rCSI 26.81%
    PRS 31.37
  • pulmonary artery endothelial cell CL1001568
    CSI 9.67
    rCSI 13.16%
    PRS 28.93
  • M cell of gut CL0000682
    CSI 8.95
    rCSI 9.51%
    PRS 32.9
  • double negative thymocyte CL0002489
    CSI 8.95
    rCSI 6.22%
    PRS 22.86
  • primitive red blood cell CL0002355
    CSI 7.59
    rCSI 40.93%
    PRS 33.55
  • retinal ganglion cell CL0000740
    CSI 7.52
    rCSI 16.62%
    PRS 13.73
  • melanocyte CL0000148
    CSI 7.35
    rCSI 5.45%
    PRS 16.67
  • naive T cell CL0000898
    CSI 7.16
    rCSI 4.98%
    PRS 27.09
  • alveolar type 1 fibroblast cell CL4028004
    CSI 7.07
    rCSI 7.75%
    PRS 21.63
  • cardiac muscle cell CL0000746
    CSI 6.89
    rCSI 9.89%
    PRS 14.84
  • vasa recta descending limb cell CL1001285
    CSI 6.84
    rCSI 54.57%
    PRS 63.35
  • lung endothelial cell CL1001567
    CSI 6.66
    rCSI 15.54%
    PRS 43.88
  • central memory CD8-positive, alpha-beta T cell CL0000907
    CSI 6.07
    rCSI 4.09%
    PRS 23.41
  • cerebral cortex neuron CL0010012
    CSI 6.07
    rCSI 24.72%
    PRS 19.51
  • podocyte CL0000653
    CSI 5.82
    rCSI 25.88%
    PRS 18.77
  • smooth muscle cell of the pulmonary artery CL0002591
    CSI 5.74
    rCSI 44.03%
    PRS 51.79
  • platelet CL0000233
    CSI 5.4
    rCSI 22.4%
    PRS 34.85
  • P/D1 enteroendocrine cell CL0002268
    CSI 5.31
    rCSI 28.92%
    PRS 44.2
  • midzonal region hepatocyte CL0019028
    CSI 5.23
    rCSI 12.27%
    PRS 27.59
  • respiratory suprabasal cell CL4033048
    CSI 5.19
    rCSI 6.66%
    PRS 22.15
  • smooth muscle cell CL0000192
    CSI 5.15
    rCSI 12.28%
    PRS 48.61
  • placental villous trophoblast CL2000060
    CSI 5.07
    rCSI 7.84%
    PRS 17.94
  • lung interstitial macrophage CL4033043
    CSI 4.87
    rCSI 10.93%
    PRS 37.69
  • unswitched memory B cell CL0000970
    CSI 4.86
    rCSI 4.09%
    PRS 30.37
  • bronchiolar smooth muscle cell CL4033017
    CSI 4.72
    rCSI 70.8%
    PRS 52.6
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 4.49
    rCSI 7.94%
    PRS 11.25
  • activated CD4-positive, alpha-beta T cell CL0000896
    CSI 4.48
    rCSI 4.14%
    PRS 34.41
  • VIP GABAergic cortical interneuron CL4023016
    CSI 4.42
    rCSI 5.28%
    PRS 11.08
  • collagen secreting cell CL0000667
    CSI 4.37
    rCSI 25.05%
    PRS 55.48
  • stromal cell CL0000499
    CSI 4.21
    rCSI 11.83%
    PRS 25.82
  • endothelial cell of uterus CL0009095
    CSI 4.19
    rCSI 30.62%
    PRS 49.8
  • epithelial cell of lower respiratory tract CL0002632
    CSI 3.97
    rCSI 3.08%
    PRS 18.35
  • central memory CD4-positive, alpha-beta T cell CL0000904
    CSI 3.96
    rCSI 2.34%
    PRS 26.74
  • pulmonary capillary endothelial cell CL4028001
    CSI 3.89
    rCSI 7.41%
    PRS 30.31
  • goblet cell CL0000160
    CSI 3.81
    rCSI 3.6%
    PRS 20.08
  • erythroid progenitor cell CL0000038
    CSI 3.7
    rCSI 21.24%
    PRS 28.85
  • memory B cell CL0000787
    CSI 3.7
    rCSI 3.65%
    PRS 62.35
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 3.64
    rCSI 3.29%
    PRS 17.2
  • CD4-positive, alpha-beta thymocyte CL0000810
    CSI 3.6
    rCSI 2.89%
    PRS 34.2
  • CD4-positive helper T cell CL0000492
    CSI 3.58
    rCSI 2.71%
    PRS 26.23
  • hematopoietic stem cell CL0000037
    CSI 3.53
    rCSI 2.35%
    PRS 22.95
  • hepatic stellate cell CL0000632
    CSI 3.5
    rCSI 13.12%
    PRS 16.17
  • alveolar adventitial fibroblast CL4028006
    CSI 3.5
    rCSI 5.53%
    PRS 19.25
  • pancreatic D cell CL0000173
    CSI 3.49
    rCSI 3.44%
    PRS 20.71
  • brush cell CL0002204
    CSI 3.45
    rCSI 6.84%
    PRS 46
  • inflammatory macrophage CL0000863
    CSI 3.39
    rCSI 5.79%
    PRS 38.41
  • erythrocyte CL0000232
    CSI 3.37
    rCSI 7.64%
    PRS 25.94
  • epithelial cell CL0000066
    CSI 3.33
    rCSI 5.11%
    PRS 26.3
  • ionocyte CL0005006
    CSI 3.26
    rCSI 3.5%
    PRS 17.65
  • adipocyte CL0000136
    CSI 3.26
    rCSI 4.19%
    PRS 18.58
  • BEST4+ enteroycte CL4030026
    CSI 3.22
    rCSI 4.01%
    PRS 20.35
  • blood vessel smooth muscle cell CL0019018
    CSI 3.18
    rCSI 25.85%
    PRS 19.38
  • vascular associated smooth muscle cell CL0000359
    CSI 3.17
    rCSI 10.29%
    PRS 23.07
  • elicited macrophage CL0000861
    CSI 3.16
    rCSI 2.9%
    PRS 22.37
  • blood vessel endothelial cell CL0000071
    CSI 3.12
    rCSI 6.47%
    PRS 19.09
  • early lymphoid progenitor CL0000936
    CSI 3.11
    rCSI 2.73%
    PRS 21.99
  • ciliated cell CL0000064
    CSI 3.06
    rCSI 4.96%
    PRS 19.32
  • interneuron CL0000099
    CSI 3.06
    rCSI 6.15%
    PRS 14.16
  • ependymal cell CL0000065
    CSI 3
    rCSI 6.09%
    PRS 9.54
  • endocardial cell CL0002350
    CSI 2.95
    rCSI 14.13%
    PRS 23.59
  • colon epithelial cell CL0011108
    CSI 2.88
    rCSI 3.02%
    PRS 17.87
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.87
    rCSI 2%
    PRS 20.36
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 2.82
    rCSI 2.15%
    PRS 25.31
  • acinar cell CL0000622
    CSI 2.81
    rCSI 4.11%
    PRS 25.15
  • common myeloid progenitor CL0000049
    CSI 2.8
    rCSI 2.26%
    PRS 19.15
  • T follicular helper cell CL0002038
    CSI 2.76
    rCSI 2.06%
    PRS 30.36
  • keratinocyte CL0000312
    CSI 2.72
    rCSI 2.28%
    PRS 22.83
  • colonocyte CL1000347
    CSI 2.63
    rCSI 3.77%
    PRS 26
  • lung microvascular endothelial cell CL2000016
    CSI 2.57
    rCSI 49.58%
    PRS 52.86
  • respiratory goblet cell CL0002370
    CSI 2.53
    rCSI 27.51%
    PRS 36.36
  • cerebral cortex endothelial cell CL1001602
    CSI 2.52
    rCSI 4.36%
    PRS 14.61
  • pulmonary alveolar type 2 cell CL0002063
    CSI 2.51
    rCSI 3.9%
    PRS 28.59
  • type B pancreatic cell CL0000169
    CSI 2.5
    rCSI 5.54%
    PRS 17.71
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 2.43
    rCSI 2.39%
    PRS 29.87
  • glioblast CL0000030
    CSI 2.42
    rCSI 3.87%
    PRS 16.41
  • CD8-positive, alpha-beta thymocyte CL0000811
    CSI 2.38
    rCSI 3.71%
    PRS 43.77
  • CD14-positive, CD16-positive monocyte CL0002397
    CSI -3.6
    rCSI -4.8%
    PRS 27.3%
  • squamous epithelial cell CL0000076
    CSI -1.0
    rCSI -2.3%
    PRS 23.6%
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 0.1
    rCSI 0.4%
    PRS 31.5%
  • sncg GABAergic cortical interneuron CL4023015
    CSI 0.1
    rCSI 0.2%
    PRS 12.3%
  • retinal cone cell CL0000573
    CSI 0.1
    rCSI 0.2%
    PRS 14.7%
  • osteoblast CL0000062
    CSI 0.2
    rCSI 4.4%
    PRS 80.9%
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.2
    rCSI 0.7%
    PRS 10.6%
  • cytotoxic T cell CL0000910
    CSI 0.2
    rCSI 1.2%
    PRS 28.1%
  • enteroglial cell CL4040002
    CSI 0.2
    rCSI 1.2%
    PRS 32.6%
  • megakaryocyte progenitor cell CL0000553
    CSI 0.2
    rCSI 4.4%
    PRS 53.0%
  • amacrine cell CL0000561
    CSI 0.3
    rCSI 0.7%
    PRS 14.7%
  • lung goblet cell CL1000143
    CSI 0.3
    rCSI 3.0%
    PRS 56.6%
  • regular ventricular cardiac myocyte CL0002131
    CSI 0.3
    rCSI 1.7%
    PRS 14.9%
  • periportal region hepatocyte CL0019026
    CSI 0.3
    rCSI 1.1%
    PRS 25.9%
  • renal principal cell CL0005009
    CSI 0.3
    rCSI 0.8%
    PRS 24.7%
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 0.3
    rCSI 0.9%
    PRS 13.0%
  • group 2 innate lymphoid cell CL0001069
    CSI 0.3
    rCSI 1.7%
    PRS 59.1%
  • tracheobronchial serous cell CL0019001
    CSI 0.3
    rCSI 1.5%
    PRS 35.2%
  • endothelial cell of placenta CL0009092
    CSI 0.4
    rCSI 1.7%
    PRS 26.0%
  • dendritic cell, human CL0001056
    CSI 0.4
    rCSI 0.5%
    PRS 22.8%
  • regular atrial cardiac myocyte CL0002129
    CSI 0.4
    rCSI 1.1%
    PRS 20.1%
  • alternatively activated macrophage CL0000890
    CSI 0.4
    rCSI 0.5%
    PRS 29.4%
  • transit amplifying cell CL0009010
    CSI 0.4
    rCSI 0.6%
    PRS 30.9%
  • colon macrophage CL0009038
    CSI 0.4
    rCSI 1.8%
    PRS 39.4%
  • L6b glutamatergic cortical neuron CL4023038
    CSI 0.4
    rCSI 1.3%
    PRS 12.1%
  • intermediate monocyte CL0002393
    CSI 0.4
    rCSI 0.6%
    PRS 19.3%
  • enteroendocrine cell of colon CL0009042
    CSI 0.4
    rCSI 1.9%
    PRS 47.4%
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 0.4
    rCSI 1.1%
    PRS 24.8%
  • ventricular cardiac muscle cell CL2000046
    CSI 0.4
    rCSI 1.4%
    PRS 63.6%
  • sst GABAergic cortical interneuron CL4023017
    CSI 0.5
    rCSI 0.6%
    PRS 11.8%
  • luminal epithelial cell of mammary gland CL0002326
    CSI 0.5
    rCSI 0.8%
    PRS 29.2%
  • peptic cell CL0000155
    CSI 0.5
    rCSI 4.6%
    PRS 49.7%
  • kidney connecting tubule epithelial cell CL1000768
    CSI 0.5
    rCSI 1.2%
    PRS 14.5%
  • astrocyte of the cerebral cortex CL0002605
    CSI 0.5
    rCSI 1.1%
    PRS 11.8%
  • T-helper 17 cell CL0000899
    CSI 0.5
    rCSI 0.4%
    PRS 33.9%
  • pluripotent stem cell CL0002248
    CSI 0.5
    rCSI 14.9%
    PRS 42.0%
  • memory T cell CL0000813
    CSI 0.5
    rCSI 1.0%
    PRS 42.8%
  • basal cell of epithelium of trachea CL1000348
    CSI 0.5
    rCSI 3.6%
    PRS 52.2%
  • renal interstitial pericyte CL1001318
    CSI 0.5
    rCSI 1.5%
    PRS 17.7%
  • metallothionein-positive alveolar macrophage CL4033042
    CSI 0.5
    rCSI 5.8%
    PRS 61.9%
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 0.5
    rCSI 1.1%
    PRS 31.9%
  • mature B cell CL0000785
    CSI 0.6
    rCSI 0.5%
    PRS 24.0%
  • hepatocyte CL0000182
    CSI 0.6
    rCSI 1.0%
    PRS 17.7%
  • luminal cell of prostate epithelium CL0002340
    CSI 0.6
    rCSI 3.1%
    PRS 33.9%
  • corneal epithelial cell CL0000575
    CSI 0.6
    rCSI 1.7%
    PRS 33.6%
  • forebrain radial glial cell CL0013000
    CSI 0.6
    rCSI 1.9%
    PRS 27.5%
  • smooth muscle cell of prostate CL1000487
    CSI 0.6
    rCSI 3.6%
    PRS 59.7%
  • myeloid lineage restricted progenitor cell CL0000839
    CSI 0.6
    rCSI 3.2%
    PRS 37.1%
  • paneth cell CL0000510
    CSI 0.6
    rCSI 0.9%
    PRS 29.9%
  • enteroendocrine cell of small intestine CL0009006
    CSI 0.6
    rCSI 1.4%
    PRS 29.1%
  • lung secretory cell CL1000272
    CSI 0.6
    rCSI 1.6%
    PRS 17.7%
  • large pre-B-II cell CL0000957
    CSI 0.7
    rCSI 1.9%
    PRS 32.5%
  • prostate gland microvascular endothelial cell CL2000059
    CSI 0.7
    rCSI 15.9%
    PRS 56.0%
  • myeloid dendritic cell, human CL0001057
    CSI 0.7
    rCSI 3.8%
    PRS 55.6%
  • kidney epithelial cell CL0002518
    CSI 0.7
    rCSI 1.3%
    PRS 42.0%
  • mesodermal cell CL0000222
    CSI 0.7
    rCSI 0.8%
    PRS 18.7%
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 0.7
    rCSI 1.6%
    PRS 19.8%
  • mucous neck cell CL0000651
    CSI 0.7
    rCSI 1.0%
    PRS 30.3%
  • tracheal goblet cell CL1000329
    CSI 0.7
    rCSI 1.5%
    PRS 37.1%
  • syncytiotrophoblast cell CL0000525
    CSI 0.7
    rCSI 2.0%
    PRS 35.7%
  • endothelial cell of venule CL1000414
    CSI 0.7
    rCSI 6.5%
    PRS 61.5%
  • multi-ciliated epithelial cell CL0005012
    CSI 0.8
    rCSI 0.8%
    PRS 16.3%
  • plasmablast CL0000980
    CSI 0.8
    rCSI 0.6%
    PRS 23.1%
  • mesenchymal cell CL0008019
    CSI 0.8
    rCSI 2.1%
    PRS 19.3%
  • tissue-resident macrophage CL0000864
    CSI 0.8
    rCSI 3.9%
    PRS 38.1%
  • CD1c-positive myeloid dendritic cell CL0002399
    CSI 0.8
    rCSI 1.0%
    PRS 22.9%
  • mesenchymal stem cell of adipose tissue CL0002570
    CSI 0.8
    rCSI 4.7%
    PRS 48.2%
  • pancreatic acinar cell CL0002064
    CSI 0.8
    rCSI 1.1%
    PRS 21.0%
  • small intestine goblet cell CL1000495
    CSI 0.9
    rCSI 1.9%
    PRS 25.6%
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 0.9
    rCSI 2.1%
    PRS 11.1%
  • mammary gland epithelial cell CL0002327
    CSI 0.9
    rCSI 3.0%
    PRS 33.3%
  • renal beta-intercalated cell CL0002201
    CSI 0.9
    rCSI 2.1%
    PRS 22.1%
  • germinal center B cell CL0000844
    CSI 0.9
    rCSI 2.6%
    PRS 43.8%
  • neural progenitor cell CL0011020
    CSI 0.9
    rCSI 3.9%
    PRS 18.1%
  • radial glial cell CL0000681
    CSI 0.9
    rCSI 1.3%
    PRS 19.6%
  • muscle cell CL0000187
    CSI 0.9
    rCSI 1.9%
    PRS 41.4%
  • myoepithelial cell CL0000185
    CSI 0.9
    rCSI 2.4%
    PRS 23.7%
  • odontoblast CL0000060
    CSI 1.0
    rCSI 21.6%
    PRS 66.2%
  • common dendritic progenitor CL0001029
    CSI 1.0
    rCSI 1.2%
    PRS 24.8%
  • vasa recta ascending limb cell CL1001131
    CSI 1.0
    rCSI 4.4%
    PRS 57.7%
  • OFF-bipolar cell CL0000750
    CSI 1.0
    rCSI 1.3%
    PRS 30.0%
  • mucus secreting cell CL0000319
    CSI 1.0
    rCSI 1.6%
    PRS 24.6%
  • mesenchymal lymphangioblast CL0005021
    CSI 1.0
    rCSI 26.4%
    PRS 68.1%
  • myeloid dendritic cell CL0000782
    CSI 1.0
    rCSI 1.5%
    PRS 28.8%
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 1.1
    rCSI 1.2%
    PRS 16.8%
  • paneth cell of epithelium of small intestine CL1000343
    CSI 1.1
    rCSI 3.0%
    PRS 29.6%
  • intestinal crypt stem cell of colon CL0009043
    CSI 1.1
    rCSI 8.0%
    PRS 34.8%
  • pulmonary alveolar type 1 cell CL0002062
    CSI 1.1
    rCSI 6.3%
    PRS 24.5%
  • endothelial cell of vascular tree CL0002139
    CSI 1.1
    rCSI 6.0%
    PRS 30.1%
  • bronchial goblet cell CL1000312
    CSI 1.1
    rCSI 4.4%
    PRS 40.2%
  • adventitial cell CL0002503
    CSI 1.1
    rCSI 2.7%
    PRS 29.0%
  • small pre-B-II cell CL0000954
    CSI 1.1
    rCSI 1.1%
    PRS 38.7%
  • glandular epithelial cell CL0000150
    CSI 1.1
    rCSI 3.0%
    PRS 37.0%
  • retina horizontal cell CL0000745
    CSI 1.1
    rCSI 1.7%
    PRS 17.8%
  • type L enteroendocrine cell CL0002279
    CSI 1.2
    rCSI 2.2%
    PRS 37.3%
  • renal alpha-intercalated cell CL0005011
    CSI 1.2
    rCSI 1.6%
    PRS 25.0%
  • pre-conventional dendritic cell CL0002010
    CSI 1.2
    rCSI 15.8%
    PRS 54.9%
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 1.2
    rCSI 0.9%
    PRS 17.7%
  • endothelial cell of pericentral hepatic sinusoid CL0019022
    CSI 1.2
    rCSI 3.7%
    PRS 28.5%
  • respiratory hillock cell CL4030023
    CSI 1.2
    rCSI 2.2%
    PRS 31.8%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.

Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CYB5R3](/details-gene/1727) encodes Cytochrome b5 reductase 3, an FAD-dependent enzyme essential for multiple metabolic pathways. The protein is a key component of the electron transport chain in the endoplasmic reticulum and mitochondria, where it participates in processes such as cholesterol and fatty acid biosynthesis, drug metabolism, and the reduction of methemoglobin. **Overall**, expression data indicates that [CYB5R3](/details-gene/1727) is a broadly expressed gene with high significance in metabolically active structural, endocrine, and endothelial cells, including [skin fibroblasts](/details-cell/CL0002620), [pancreatic A cells](/details-cell/CL0000171), and various endothelial cell types. Clinically, mutations in [CYB5R3](/details-gene/1727) are associated with Type I and Type II recessive congenital methemoglobinemia ([OMIM: 250800](https://omim.org/entry/250800)). ## Cellular Roles and Expression Landscape The expression profile of [CYB5R3](/details-gene/1727) suggests a fundamental role in cellular metabolism rather than serving as a marker for a specific cell lineage. Its high significance across a diverse array of cell types underscores its housekeeping function in maintaining cellular redox balance and supporting biosynthetic processes. **Overall**, the gene shows the highest significance in cells with high metabolic or secretory activity. This includes mesenchymal cells such as [skin fibroblast](/details-cell/CL0002620) (CSI: 32.16) and [bronchus fibroblast of lung](/details-cell/CL2000093) (CSI: 23.78), as well as contractile cells like [microcirculation associated smooth muscle cell](/details-cell/CL0008035) (CSI: 23.63). High significance is also observed in endocrine and epithelial cells, including [pancreatic A cell](/details-cell/CL0000171) (CSI: 28.01) and [intestinal epithelial cell](/details-cell/CL0002563) (CSI: 21.36). Furthermore, its notable expression in vascular cells like [retinal blood vessel endothelial cell](/details-cell/CL0002585) (CSI: 17.95) and [endothelial cell of artery](/details-cell/CL1000413) (CSI: 17.19) is consistent with its role in nitric oxide biosynthesis and lipid metabolism, critical for vascular homeostasis. Conversely, [CYB5R3](/details-gene/1727) shows low to negative significance in certain immune cell subsets, such as the [CD14-positive, CD16-positive monocyte](/details-cell/CL0002397) (CSI: -3.64). This pattern suggests that while the gene is involved in general immune system processes, its expression level may be less critical or differentially regulated in specific circulating monocyte populations compared to tissue-resident cells. ## Pathways and Molecular Function [CYB5R3](/details-gene/1727) functions as a NADH-dependent oxidoreductase, primarily exerting its [cytochrome-b5 reductase activity](/details-gene/GO:0004128). Its molecular functions are centered around electron transfer and binding cofactors such as [FAD](/details-gene/GO:0071949), [NAD](/details-gene/GO:0051287), and [ADP](/details-gene/GO:0043531). The gene is integral to several core metabolic pathways as annotated by Reactome. It is a key player in [Biological oxidations](/details-gene/R-HSA-211859) and [Phase I - functionalization of compounds](/details-gene/R-HSA-211945), highlighting its role in detoxification and metabolism of xenobiotics. Its involvement in the [cholesterol biosynthetic process](/details-gene/GO:0006695) is consistent with its high expression in various metabolically active tissues. Furthermore, its participation in the [nitric oxide biosynthetic process](/details-gene/GO:0006809) provides a molecular basis for its importance in the vascular endothelium. Cellular component annotations place the protein in multiple subcellular locations, including the [endoplasmic reticulum membrane](/details-gene/GO:0005789), [mitochondrial outer membrane](/details-gene/GO:0005741), and the [cytosol](/details-gene/GO:0005829), which aligns with its diverse metabolic functions. Functional annotation also connects [CYB5R3](/details-gene/1727) to the [immune system](/details-gene/R-HSA-168256), specifically in the context of [neutrophil degranulation](/details-gene/R-HSA-6798695). ## Research Directions The widespread expression of [CYB5R3](/details-gene/1727) suggests that its dysregulation could have systemic consequences. Future research should focus on dissecting its cell-type-specific roles in health and disease. **Proposed Hypotheses:** 1. Given its role in the [nitric oxide biosynthetic process](/details-gene/GO:0006809) and high expression in endothelial cells, dysfunction of [CYB5R3](/details-gene/1727) in the vascular endothelium may be a contributing factor to endothelial dysfunction, a key initiating event in atherosclerosis. Reduced reductase activity could impair nitric oxide production, leading to decreased vasodilation and increased inflammation. 2. The annotation for [neutrophil degranulation](/details-gene/R-HSA-6798695) suggests a role in innate immunity that may not be captured by its expression level in circulating monocytes. It is hypothesized that [CYB5R3](/details-gene/1727) is critical for generating reactive oxygen species (ROS) or for lipid mediator biosynthesis in neutrophils during phagocytosis and inflammatory responses. **Experimental Approach:** To test the first hypothesis regarding the role of [CYB5R3](/details-gene/1727) in endothelial dysfunction, a targeted knockout of the gene could be generated in primary human endothelial cells (e.g., HUVECs) using CRISPR-Cas9. The functional consequences would be assessed by measuring nitric oxide production in response to stimuli like shear stress or acetylcholine. Additionally, the cellular response to inflammatory cytokines (e.g., TNF-alpha) could be evaluated by quantifying the expression of adhesion molecules (ICAM-1, VCAM-1) and the cellular lipidome via mass spectrometry to investigate links to lipid metabolism. **Therapeutic Potential:** Given that loss-of-function mutations cause congenital methemoglobinemia, [CYB5R3](/details-gene/1727) is not a suitable target for inhibition. Therapeutic strategies would likely focus on restoring or enhancing its function in deficiency states. Its broad, constitutive expression pattern suggests that systemic inhibition would lead to severe, widespread toxicity by disrupting fundamental metabolic processes in nearly all tissues. Therefore, therapeutic interest would be primarily in the realm of gene replacement therapy or the development of pharmacological chaperones for specific patient mutations, rather than as a target for traditional small-molecule inhibitors in complex diseases.

Genular Protein ID: 1683685757

Symbol: NB5R3_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 2479590

Title: The organization and the complete nucleotide sequence of the human NADH-cytochrome b5 reductase gene.

PubMed ID: 2479590

DOI: 10.1016/0378-1119(89)90299-0

PubMed ID: 15461802

Title: A genome annotation-driven approach to cloning the human ORFeome.

PubMed ID: 15461802

DOI: 10.1186/gb-2004-5-10-r84

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 10591208

Title: The DNA sequence of human chromosome 22.

PubMed ID: 10591208

DOI: 10.1038/990031

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 3035541

Title: Molecular cloning of cDNAs of human liver and placenta NADH-cytochrome b5 reductase.

PubMed ID: 3035541

DOI: 10.1073/pnas.84.11.3609

PubMed ID: 2498303

Title: The NH2-terminal structures of human and rat liver microsomal NADH-cytochrome b5 reductases.

PubMed ID: 2498303

DOI: 10.1093/oxfordjournals.jbchem.a122659

PubMed ID: 3700359

Title: Complete amino acid sequence of NADH-cytochrome b5 reductase purified from human erythrocytes.

PubMed ID: 3700359

DOI: 10.1093/oxfordjournals.jbchem.a135495

PubMed ID: 6389526

Title: Amino acid sequence of NADH-cytochrome b5 reductase of human erythrocytes.

PubMed ID: 6389526

DOI: 10.1093/oxfordjournals.jbchem.a134871

PubMed ID: 9639531

Title: An erythroid-specific transcript generates the soluble form of NADH-cytochrome b5 reductase in humans.

PubMed ID: 9639531

PubMed ID: 2019583

Title: Role of cysteine residues in human NADH-cytochrome b5 reductase studied by site-directed mutagenesis. Cys-273 and Cys-283 are located close to the NADH-binding site but are not catalytically essential.

PubMed ID: 2019583

DOI: 10.1016/s0021-9258(20)89479-4

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 25255805

Title: Global profiling of co- and post-translationally N-myristoylated proteomes in human cells.

PubMed ID: 25255805

DOI: 10.1038/ncomms5919

PubMed ID: 25944712

Title: N-terminome analysis of the human mitochondrial proteome.

PubMed ID: 25944712

DOI: 10.1002/pmic.201400617

PubMed ID: 15502298

Title: Structure of human erythrocyte NADH-cytochrome b5 reductase.

PubMed ID: 15502298

DOI: 10.1107/s0907444904020645

PubMed ID: 1898726

Title: Structural role of serine 127 in the NADH-binding site of human NADH-cytochrome b5 reductase.

PubMed ID: 1898726

DOI: 10.1016/s0021-9258(18)52402-9

PubMed ID: 1707593

Title: Exonic point mutations in NADH-cytochrome B5 reductase genes of homozygotes for hereditary methemoglobinemia, types I and III: putative mechanisms of tissue-dependent enzyme deficiency.

PubMed ID: 1707593

PubMed ID: 1400360

Title: Enzymatic instability of NADH-cytochrome b5 reductase as a cause of hereditary methemoglobinemia type I (red cell type).

PubMed ID: 1400360

DOI: 10.1016/s0021-9258(19)88718-5

PubMed ID: 8119939

Title: An in-frame deletion of codon 298 of the NADH-cytochrome b5 reductase gene results in hereditary methemoglobinemia type II (generalized type). A functional implication for the role of the COOH-terminal region of the enzyme.

PubMed ID: 8119939

DOI: 10.1016/s0021-9258(17)37554-3

PubMed ID: 7718898

Title: Four new mutations in the NADH-cytochrome b5 reductase gene from patients with recessive congenital methemoglobinemia type II.

PubMed ID: 7718898

PubMed ID: 9048929

Title: A high-frequency polymorphism of NADH-cytochrome b5 reductase in African-Americans.

PubMed ID: 9048929

DOI: 10.1007/s004390050347

PubMed ID: 9695975

Title: Identification of a novel point mutation (Leu72-to-Pro) in the NADH-cytochrome b5 reductase gene of a patient with hereditary methaemoglobinaemia type I.

PubMed ID: 9695975

DOI: 10.1046/j.1365-2141.1998.00782.x

PubMed ID: 9886302

Title: Molecular basis of hereditary methaemoglobinaemia, types I and II: two novel mutations in the NADH-cytochrome b5 reductase gene.

PubMed ID: 9886302

DOI: 10.1046/j.1365-2141.1998.01123.x

PubMed ID: 10807796

Title: A novel mutation in the NADH-cytochrome b5 reductase gene of a Chinese patient with recessive congenital methemoglobinemia.

PubMed ID: 10807796

PubMed ID: 15622768

Title: Arginine-glutamine replacement at residue 57 of NADH-cytochrome b5 reductase in Chinese hereditary methemoglobinemia.

PubMed ID: 15622768

PubMed ID: 12393396

Title: Familial idiopathic methemoglobinemia revisited: original cases reveal 2 novel mutations in NADH-cytochrome b5 reductase.

PubMed ID: 12393396

DOI: 10.1182/blood-2002-05-1405

PubMed ID: 15953014

Title: Recessive congenital methaemoglobinaemia: functional characterization of the novel D239G mutation in the NADH-binding lobe of cytochrome b5 reductase.

PubMed ID: 15953014

DOI: 10.1111/j.1365-2141.2005.05526.x

Sequence Information:

  • Length: 301
  • Mass: 34235
  • Checksum: FDCDCDC4EC3570B4
  • Sequence:
  • MGAQLSTLGH MVLFPVWFLY SLLMKLFQRS TPAITLESPD IKYPLRLIDR EIISHDTRRF 
    RFALPSPQHI LGLPVGQHIY LSARIDGNLV VRPYTPISSD DDKGFVDLVI KVYFKDTHPK 
    FPAGGKMSQY LESMQIGDTI EFRGPSGLLV YQGKGKFAIR PDKKSNPIIR TVKSVGMIAG 
    GTGITPMLQV IRAIMKDPDD HTVCHLLFAN QTEKDILLRP ELEELRNKHS ARFKLWYTLD 
    RAPEAWDYGQ GFVNEEMIRD HLPPPEEEPL VLMCGPPPMI QYACLPNLDH VGHPTERCFV 
    F