Details for: KIFC1

Gene ID: 3833

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: KIFC1

Ensembl ID: ENSG00000237649

Description: kinesin family member C1

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • large pre-B-II cell CL0000957
    CSI 4.89
    rCSI 13.96%
    PRS 96.75
  • neural crest cell CL0011012
    CSI 4.01
    rCSI 3.17%
    PRS 95.29
  • epithelial cell of lung CL0000082
    CSI 3.68
    rCSI 3.05%
    PRS 98.1
  • common myeloid progenitor CL0000049
    CSI 3.64
    rCSI 2.94%
    PRS 98.38
  • fallopian tube secretory epithelial cell CL4030006
    CSI 3.56
    rCSI 3.42%
    PRS 96.63
  • pro-B cell CL0000826
    CSI 3.52
    rCSI 2.92%
    PRS 98.06
  • plasmablast CL0000980
    CSI 3.08
    rCSI 2.42%
    PRS 97.5
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.71
    rCSI 3.13%
    PRS 93.65
  • erythrocyte CL0000232
    CSI 2.69
    rCSI 6.1%
    PRS 95.86
  • mesodermal cell CL0000222
    CSI 2.54
    rCSI 3.05%
    PRS 97.81
  • stem cell CL0000034
    CSI 2.52
    rCSI 2.43%
    PRS 96.19
  • fraction A pre-pro B cell CL0002045
    CSI 2.48
    rCSI 2.84%
    PRS 98.83
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.39
    rCSI 3.06%
    PRS 95.83
  • glioblast CL0000030
    CSI 2.28
    rCSI 3.63%
    PRS 93.81
  • transit amplifying cell of colon CL0009011
    CSI 2.2
    rCSI 2.58%
    PRS 97.73
  • placental villous trophoblast CL2000060
    CSI 1.99
    rCSI 3.08%
    PRS 96.42
  • germinal center B cell CL0000844
    CSI 1.72
    rCSI 5.14%
    PRS 98.86
  • promonocyte CL0000559
    CSI 1.66
    rCSI 2.84%
    PRS 98.12
  • erythroblast CL0000765
    CSI 1.58
    rCSI 4.2%
    PRS 97.03
  • mesenchymal cell CL0008019
    CSI 1.05
    rCSI 2.67%
    PRS 96.08

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [KIFC1](/details-gene/3833), or Kinesin Family Member C1, is a protein-coding gene located on chromosome 6p21.32. It encodes a C-terminal-type kinesin-related motor protein that moves towards the minus-end of microtubules. Its primary functions are associated with intracellular transport and cell division, particularly in the organization and stabilization of the mitotic spindle. **Overall**, expression data reveals that [KIFC1](/details-gene/3833) is most significant in highly proliferative cell populations, including hematopoietic progenitors, neural precursors, and various stem cells, suggesting a critical role in the rapid cell cycles that characterize development and tissue renewal. ## Cellular Roles and Expression Landscape The expression pattern of [KIFC1](/details-gene/3833) underscores its central role in cellular proliferation and differentiation across diverse lineages. Its highest significance is observed in B-lymphocyte precursors, such as the [large pre-B-II cell](/details-cell/CL0000957) (CSI: 4.89) and [pro-B cell](/details-cell/CL0000826) (CSI: 3.52), as well as in the [common myeloid progenitor](/details-cell/CL0000049) (CSI: 3.64). This enrichment in hematopoietic progenitors suggests an essential function during the rapid clonal expansion phases of immune cell development. Beyond the hematopoietic system, [KIFC1](/details-gene/3833) also shows high significance in neural development, as indicated by its prominence in [neural crest cell](/details-cell/CL0011012) (CSI: 4.01) and [neuroblast (sensu Vertebrata)](/details-cell/CL0000031) (CSI: 2.39). Further supporting its role in proliferation, it is highly significant in [stem cell](/details-cell/CL0000034) populations and transit-amplifying cells, such as [transit amplifying cell of colon](/details-cell/CL0009011) (CSI: 2.20). The gene is also notably active in specific epithelial tissues, including [epithelial cell of lung](/details-cell/CL0000082) (CSI: 3.68) and [fallopian tube secretory epithelial cell](/details-cell/CL4030006) (CSI: 3.56), which may reflect roles in both tissue maintenance and specialized transport functions. The consistent presence in these varied, actively dividing cell types solidifies the role of [KIFC1](/details-gene/3833) as a key component of the mitotic machinery. ## Pathways and Molecular Function Functionally, [KIFC1](/details-gene/3833) is annotated as a microtubule-based motor protein involved in fundamental cellular processes. Its molecular functions include [ATP binding](/details-go/GO:0005524), [ATP hydrolysis activity](/details-go/GO:0016887), and [microtubule motor activity](/details-go/GO:0003777), which collectively drive its movement along microtubules. This activity is crucial for its role in biological processes such as [cell division](/details-go/GO:0051301), specifically [mitotic spindle assembly](/details-go/GO:0090307) and [mitotic metaphase chromosome alignment](/details-go/GO:0007080). Multiple studies have confirmed its role in mitosis and cytokinesis [Link](https://doi.org/10.1091/mbc.e05-02-0167) and its status as a phosphoprotein regulated during the cell cycle [Link](https://doi.org/10.1073/pnas.0805139105). Cellular component annotations place the protein in the [cytoplasm](/details-go/GO:0005737), [centrosome](/details-go/GO:0005813), and the [mitotic spindle](/details-go/GO:0072686). Reactome pathway analysis further expands its function beyond mitosis into membrane and vesicle dynamics. [KIFC1](/details-gene/3833) is implicated in [Vesicle-mediated transport](/details-pathway/R-HSA-5653656), particularly [Intra-Golgi and retrograde Golgi-to-ER traffic](/details-pathway/R-HSA-6811442). This dual involvement in both cell division and intracellular trafficking highlights its role as a versatile motor protein essential for cellular organization. Its participation in pathways related to [hemostasis](/details-pathway/R-HSA-109582), including [Factors involved in megakaryocyte development and platelet production](/details-pathway/R-HSA-983231), is consistent with its function in microtubule-dependent processes required for platelet formation. ## Research Directions The expression profile and functional annotation of [KIFC1](/details-gene/3833) suggest several avenues for future investigation. Its prominent role in cell division makes it a protein of interest, particularly in contexts of abnormal proliferation such as cancer. Based on the available data, several testable hypotheses can be proposed: 1. **Hypothesis 1:** Given its high significance in B-cell precursors ([large pre-B-II cell](/details-cell/CL0000957), [pro-B cell](/details-cell/CL0000826)), [KIFC1](/details-gene/3833) is indispensable for the rapid mitotic divisions required for B-cell development in the bone marrow. Its inhibition would lead to a bottleneck at the pre-B cell stage. 2. **Hypothesis 2:** The high significance of [KIFC1](/details-gene/3833) in [fallopian tube secretory epithelial cell](/details-cell/CL4030006), combined with its annotated role in Golgi-to-ER transport, suggests it is critical for the secretion of proteins and lipids that constitute the oviductal fluid, thereby playing a role in creating a supportive microenvironment for fertilization and early embryonic development. To test the first hypothesis regarding its role in B-cell development, a compelling experimental approach would be to utilize a conditional knockout mouse model. Specifically, crossing a mouse with floxed *Kifc1* alleles to a mouse expressing Cre recombinase under the control of a B-cell specific promoter (e.g., *Cd19-Cre*) would delete [KIFC1](/details-gene/3833) specifically in the B-cell lineage. The resulting phenotype could be analyzed using flow cytometry of bone marrow and spleen to quantify B-cell populations at different developmental stages (pro-B, pre-B, immature B), which would directly assess whether its absence causes a developmental arrest. **Therapeutic Potential** As [KIFC1](/details-gene/3833) is essential for mitotic spindle organization, it represents a compelling therapeutic target for diseases characterized by uncontrolled cell proliferation, such as cancer. Its high expression in multiple progenitor and cancer cell types (e.g., [glioblast](/details-cell/CL0000030)) supports this notion. The development of small molecule inhibitors that specifically target the ATPase motor domain of [KIFC1](/details-gene/3833) could induce mitotic arrest and apoptosis in rapidly dividing cancer cells. Therefore, a therapeutic strategy based on **inhibition** of [KIFC1](/details-gene/3833) function could offer a targeted anti-mitotic therapy, potentially with a different toxicity profile than conventional tubulin-targeting agents.

Genular Protein ID: 2165620451

Symbol: KIFC1_HUMAN

Name: Kinesin-like protein KIFC1

UniProtKB Accession Codes:

Q9BW19 O60887 Q14834 Q4KMP0 Q5SU09 Q6GMS7 Q6P4A5 Q9UQP7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChEBI 1 ChEMBL 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 13 Ensembl 3 ExpressionAtlas 1 GO 17 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 1 PDBsum 1 PIR 1 PRINTS 1 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 2 RefSeq 1 SIGNOR 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10369922

Title: Genomic organization of the HSET locus and the possible association of HLA-linked genes with immotile cilia syndrome (ICS).

PubMed ID: 10369922

DOI: 10.1007/s002510050660

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 8276466

Title: Cloning of a new kinesin-related gene located at the centromeric end of the human MHC region.

PubMed ID: 8276466

DOI: 10.1007/bf00241260

PubMed ID: 15843429

Title: Functional analysis of human microtubule-based motor proteins, the kinesins and dyneins, in mitosis/cytokinesis using RNA interference.

PubMed ID: 15843429

DOI: 10.1091/mbc.e05-02-0167

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

Sequence Information:

  • Length: 673
  • Mass: 73748
  • Checksum: 9E99737665BF1E92
  • Sequence:
    • MDPQRSPLLE VKGNIELKRP LIKAPSQLPL SGSRLKRRPD QMEDGLEPEK KRTRGLGATT 
KITTSHPRVP SLTTVPQTQG QTTAQKVSKK TGPRCSTAIA TGLKNQKPVP AVPVQKSGTS 
GVPPMAGGKK PSKRPAWDLK GQLCDLNAEL KRCRERTQTL DQENQQLQDQ LRDAQQQVKA 
LGTERTTLEG HLAKVQAQAE QGQQELKNLR ACVLELEERL STQEGLVQEL QKKQVELQEE 
RRGLMSQLEE KERRLQTSEA ALSSSQAEVA SLRQETVAQA ALLTEREERL HGLEMERRRL 
HNQLQELKGN IRVFCRVRPV LPGEPTPPPG LLLFPSGPGG PSDPPTRLSL SRSDERRGTL 
SGAPAPPTRH DFSFDRVFPP GSGQDEVFEE IAMLVQSALD GYPVCIFAYG QTGSGKTFTM 
EGGPGGDPQL EGLIPRALRH LFSVAQELSG QGWTYSFVAS YVEIYNETVR DLLATGTRKG 
QGGECEIRRA GPGSEELTVT NARYVPVSCE KEVDALLHLA RQNRAVARTA QNERSSRSHS 
VFQLQISGEH SSRGLQCGAP LSLVDLAGSE RLDPGLALGP GERERLRETQ AINSSLSTLG 
LVIMALSNKE SHVPYRNSKL TYLLQNSLGG SAKMLMFVNI SPLEENVSES LNSLRFASKV 
NQCVIGTAQA NRK