Details for: ASNS

Gene ID: 440

Symbol: ASNS

Ensembl ID: ENSG00000070669

Description: asparagine synthetase (glutamine-hydrolyzing)

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 111.8003
    Cell Significance Index: -17.3900
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 72.9765
    Cell Significance Index: -18.5100
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 37.8292
    Cell Significance Index: -17.8600
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 36.7255
    Cell Significance Index: -14.9200
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 15.6482
    Cell Significance Index: -14.9400
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 15.4669
    Cell Significance Index: -19.0700
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 5.7412
    Cell Significance Index: -15.3800
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 4.9619
    Cell Significance Index: -19.5800
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 2.3946
    Cell Significance Index: -7.3600
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 2.1064
    Cell Significance Index: -4.6100
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 1.2855
    Cell Significance Index: 1160.7100
  • Cell Name: decidual cell (CL2000002)
    Fold Change: 1.1724
    Cell Significance Index: 18.8100
  • Cell Name: hippocampal pyramidal neuron (CL1001571)
    Fold Change: 1.1438
    Cell Significance Index: 32.6400
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.8198
    Cell Significance Index: 133.3400
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.7874
    Cell Significance Index: 77.8900
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.6949
    Cell Significance Index: 19.4200
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.6858
    Cell Significance Index: 74.5900
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.6084
    Cell Significance Index: 17.5300
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: 0.4940
    Cell Significance Index: 33.2200
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: 0.4865
    Cell Significance Index: 29.9000
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 0.4413
    Cell Significance Index: 23.1700
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: 0.3720
    Cell Significance Index: 8.1500
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.3615
    Cell Significance Index: 25.0000
  • Cell Name: skeletal muscle myoblast (CL0000515)
    Fold Change: 0.3017
    Cell Significance Index: 3.2800
  • Cell Name: cortical interneuron (CL0008031)
    Fold Change: 0.2910
    Cell Significance Index: 6.9800
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 0.2842
    Cell Significance Index: 17.0600
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: 0.2575
    Cell Significance Index: 5.3900
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.2317
    Cell Significance Index: 160.2600
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.2077
    Cell Significance Index: 113.4200
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.1883
    Cell Significance Index: 4.0800
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.1746
    Cell Significance Index: 33.2200
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.1657
    Cell Significance Index: 7.5100
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.1636
    Cell Significance Index: 32.4700
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.1621
    Cell Significance Index: 32.5200
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.1553
    Cell Significance Index: 18.3100
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.1403
    Cell Significance Index: 4.9300
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.1297
    Cell Significance Index: 22.1500
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: 0.1265
    Cell Significance Index: 6.5900
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.0991
    Cell Significance Index: 43.8000
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 0.0848
    Cell Significance Index: 2.4900
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 0.0706
    Cell Significance Index: 9.1300
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 0.0631
    Cell Significance Index: 4.4600
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.0416
    Cell Significance Index: 14.9300
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: 0.0327
    Cell Significance Index: 2.4400
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.0279
    Cell Significance Index: 0.3800
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: 0.0247
    Cell Significance Index: 18.7000
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.0131
    Cell Significance Index: 0.2800
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: 0.0096
    Cell Significance Index: 0.2000
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.0072
    Cell Significance Index: 0.1200
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: -0.0089
    Cell Significance Index: -0.3100
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0104
    Cell Significance Index: -19.5200
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: -0.0110
    Cell Significance Index: -1.5100
  • Cell Name: odontoblast (CL0000060)
    Fold Change: -0.0122
    Cell Significance Index: -1.5600
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0148
    Cell Significance Index: -10.8400
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0149
    Cell Significance Index: -27.5500
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0152
    Cell Significance Index: -11.2500
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0296
    Cell Significance Index: -16.7000
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0349
    Cell Significance Index: -21.8100
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0432
    Cell Significance Index: -19.6200
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0466
    Cell Significance Index: -4.7600
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.0497
    Cell Significance Index: -5.7900
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0540
    Cell Significance Index: -15.5500
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: -0.0566
    Cell Significance Index: -10.2000
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.0702
    Cell Significance Index: -3.3000
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: -0.0749
    Cell Significance Index: -3.4900
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.0997
    Cell Significance Index: -11.4200
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.1005
    Cell Significance Index: -21.1700
  • Cell Name: BEST4+ enteroycte (CL4030026)
    Fold Change: -0.1015
    Cell Significance Index: -1.5300
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.1141
    Cell Significance Index: -16.5800
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: -0.1144
    Cell Significance Index: -14.0700
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.1213
    Cell Significance Index: -3.1900
  • Cell Name: paneth cell of colon (CL0009009)
    Fold Change: -0.1301
    Cell Significance Index: -1.9500
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: -0.1609
    Cell Significance Index: -10.3800
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: -0.1648
    Cell Significance Index: -8.5600
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: -0.1828
    Cell Significance Index: -4.7000
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.1976
    Cell Significance Index: -15.6500
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.2043
    Cell Significance Index: -15.6800
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.2048
    Cell Significance Index: -6.5600
  • Cell Name: skeletal muscle fibroblast (CL0011027)
    Fold Change: -0.2192
    Cell Significance Index: -1.4900
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: -0.2272
    Cell Significance Index: -14.3200
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.2457
    Cell Significance Index: -6.5600
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.2493
    Cell Significance Index: -6.7900
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: -0.2657
    Cell Significance Index: -5.6400
  • Cell Name: proerythroblast (CL0000547)
    Fold Change: -0.2966
    Cell Significance Index: -4.2500
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.3138
    Cell Significance Index: -19.2400
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: -0.3147
    Cell Significance Index: -17.6600
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: -0.3256
    Cell Significance Index: -10.3700
  • Cell Name: helper T cell (CL0000912)
    Fold Change: -0.3371
    Cell Significance Index: -4.7900
  • Cell Name: hippocampal interneuron (CL1001569)
    Fold Change: -0.3374
    Cell Significance Index: -4.3800
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: -0.3484
    Cell Significance Index: -8.7100
  • Cell Name: neuroblast (sensu Vertebrata) (CL0000031)
    Fold Change: -0.3489
    Cell Significance Index: -2.2700
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: -0.3520
    Cell Significance Index: -12.3300
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: -0.3552
    Cell Significance Index: -11.6300
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: -0.3966
    Cell Significance Index: -11.3700
  • Cell Name: lamp5 GABAergic cortical interneuron (CL4023011)
    Fold Change: -0.4087
    Cell Significance Index: -8.8300
  • Cell Name: stratified epithelial cell (CL0000079)
    Fold Change: -0.4296
    Cell Significance Index: -15.7700
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: -0.4382
    Cell Significance Index: -10.9300
  • Cell Name: sst GABAergic cortical interneuron (CL4023017)
    Fold Change: -0.4440
    Cell Significance Index: -8.7800
  • Cell Name: medial ganglionic eminence derived interneuron (CL4023063)
    Fold Change: -0.4654
    Cell Significance Index: -6.6700
  • Cell Name: epithelial cell of pancreas (CL0000083)
    Fold Change: -0.4667
    Cell Significance Index: -7.6900

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** ASNS is a bifunctional enzyme that catalyzes two distinct reactions: the synthesis of asparagine from glutamine and the hydrolysis of asparagine to glutamine. This enzyme is predominantly expressed in cells that require high levels of asparagine, such as pancreatic A cells, which are responsible for insulin production. ASNS is also found in other cell types, including pulmonary interstitial fibroblasts, male germ cells, and dopaminergic neurons. **Pathways and Functions:** ASNS is intricately involved in various cellular pathways, including: 1. **Amino acid metabolism:** ASNS plays a crucial role in the biosynthesis of asparagine, which is essential for protein synthesis, cell growth, and differentiation. 2. **Stress responses:** ASNS is activated in response to endoplasmic reticulum (ER) stress, a condition characterized by the accumulation of unfolded proteins. This activation leads to the attenuation of the unfolded protein response (UPR), a stress response mechanism that helps maintain cellular homeostasis. 3. **Cell cycle regulation:** ASNS is involved in the regulation of the mitotic cell cycle, with its activity influencing the progression of cells from the G1 to the S phase. 4. **Apoptosis regulation:** ASNS negatively regulates the apoptotic process, a mechanism of programmed cell death that helps eliminate damaged or dysfunctional cells. **Clinical Significance:** The dysregulation of ASNS has been implicated in various human diseases, including: 1. **Diabetes:** ASNS deficiency has been linked to diabetes, as impaired asparagine synthesis can disrupt insulin production and secretion. 2. **Neurodegenerative disorders:** ASNS has been implicated in neurodegenerative diseases, such as Parkinson's disease, where its dysregulation can contribute to the accumulation of toxic proteins. 3. **Cancer:** ASNS has been shown to be overexpressed in certain types of cancer, where its activity can promote cell proliferation and survival. In conclusion, ASNS is a multifunctional enzyme that plays a crucial role in various cellular pathways, including amino acid metabolism, stress responses, and cell cycle regulation. Its dysregulation has been implicated in various human diseases, highlighting the importance of ASNS in maintaining cellular homeostasis and overall health. Further research is needed to fully elucidate the mechanisms underlying ASNS function and its implications in human disease.

Genular Protein ID: 3619525398

Symbol: ASNS_HUMAN

Name: Asparagine synthetase [glutamine-hydrolyzing]

UniProtKB Accession Codes:

P08243 A4D1I8 B4DXZ1 B7ZAA9 D6W5R3 E9PCI3 E9PCX6 P08184 Q15666 Q549T9 Q96HD0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 3 CDD 2 CTD 1 ChEBI 15 ChEMBL 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 5 DrugCentral 1 EC 2 EMBL 27 EMDB 1 Ensembl 7 ExpressionAtlas 1 GO 9 Gene3D 2 GeneCards 1 GeneReviews 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 7 KEGG 1 MANE-Select 1 MEROPS 1 MIM 3 MINT 1 MalaCards 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 NCBIfam 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 2 PDBsum 2 PIR 1 PIRSF 1 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 Reactome 4 RefSeq 6 Rhea 1 SIGNOR 1 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 UniPathway 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 2886907

Title: Isolation of human cDNAs for asparagine synthetase and expression in Jensen rat sarcoma cells.

PubMed ID: 2886907

DOI: 10.1128/mcb.7.7.2435-2443.1987

PubMed ID: 2565875

Title: Molecular structure of the human asparagine synthetase gene.

PubMed ID: 2565875

DOI: 10.1016/0888-7543(89)90329-7

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 12690205

Title: Human chromosome 7: DNA sequence and biology.

PubMed ID: 12690205

DOI: 10.1126/science.1083423

PubMed ID: 12853948

Title: The DNA sequence of human chromosome 7.

PubMed ID: 12853948

DOI: 10.1038/nature01782

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 3470743

Title: Molecular cloning of a gene that is necessary for G1 progression in mammalian cells.

PubMed ID: 3470743

DOI: 10.1073/pnas.84.6.1565

PubMed ID: 2569668

Title: Organization and expression of the cell cycle gene, ts11, that encodes asparagine synthetase.

PubMed ID: 2569668

DOI: 10.1128/mcb.9.6.2350-2359.1989

PubMed ID: 2564390

Title: Expression of human asparagine synthetase in Escherichia coli.

PubMed ID: 2564390

DOI: 10.1016/s0021-9258(18)83573-6

PubMed ID: 2573597

Title: The N-terminal cysteine of human asparagine synthetase is essential for glutamine-dependent activity.

PubMed ID: 2573597

DOI: 10.1016/s0021-9258(19)47138-x

PubMed ID: 14654843

Title: Proteomic characterization of the human centrosome by protein correlation profiling.

PubMed ID: 14654843

DOI: 10.1038/nature02166

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24139043

Title: Deficiency of asparagine synthetase causes congenital microcephaly and a progressive form of encephalopathy.

PubMed ID: 24139043

DOI: 10.1016/j.neuron.2013.08.013

Sequence Information:

  • Length: 561
  • Mass: 64370
  • Checksum: 3E9B75E21D799FBE
  • Sequence:
    • MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL AVVDPLFGMQ 
PIRVKKYPYL WLCYNGEIYN HKKMQQHFEF EYQTKVDGEI ILHLYDKGGI EQTICMLDGV 
FAFVLLDTAN KKVFLGRDTY GVRPLFKAMT EDGFLAVCSE AKGLVTLKHS ATPFLKVEPF 
LPGHYEVLDL KPNGKVASVE MVKYHHCRDV PLHALYDNVE KLFPGFEIET VKNNLRILFN 
NAVKKRLMTD RRIGCLLSGG LDSSLVAATL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR 
KVADHIGSEH YEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK YIRKNTDSVV 
IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLRELYLF DVLRADRTTA AHGLELRVPF 
LDHRFSSYYL SLPPEMRIPK NGIEKHLLRE TFEDSNLIPK EILWRPKEAF SDGITSVKNS 
WFKILQEYVE HQVDDAMMAN AAQKFPFNTP KTKEGYYYRQ VFERHYPGRA DWLSHYWMPK 
WINATDPSAR TLTHYKSAVK A

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.