Details for: MIS18A AS1

Gene ID: 100874202

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: MIS18A AS1

Ensembl ID: ENSG00000227256

Description: MIS18A antisense RNA 1

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • neural progenitor cell CL0011020
    CSI 11.49
    rCSI 50.54%
    PRS 99.9

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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    • High
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    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [MIS18A AS1](/details-gene/100874202), or MIS18A antisense RNA 1, is a non-coding RNA located on chromosome 21q22.11. As an antisense transcript to the protein-coding gene MIS18A, it is hypothesized to be involved in the regulation of its sense counterpart, which plays a critical role in centromere propagation and kinetochore assembly during mitosis. Expression data indicates that [MIS18A AS1](/details-gene/100874202) has a highly specific expression profile, suggesting it may function as a defining marker for [neural progenitor cell](/details-cell/CL0011020). ## Cellular Roles and Expression Landscape The expression profile of [MIS18A AS1](/details-gene/100874202) is characterized by its remarkable specificity. **Overall**, the gene exhibits its most significant expression in [neural progenitor cell](/details-cell/CL0011020) (CSI: 11.49). This high cell significance index suggests that [MIS18A AS1](/details-gene/100874202) is not merely expressed but may serve as a specific molecular marker that distinguishes this cell population. The absence of significant expression in other cell types within the available data implies a highly specialized role, likely tied to the unique biological processes of neural progenitors, such as proliferation, self-renewal, and differentiation during neurogenesis. ## Pathways and Molecular Function While direct functional annotation is not available, the gene's designation as an antisense RNA to MIS18A provides a strong basis for its hypothesized function. Antisense non-coding RNAs typically regulate the expression of their corresponding sense gene through mechanisms like transcriptional interference, mRNA degradation, or translational repression. The sense gene, MIS18A, is a core component of the Mis18 complex, which is essential for the recruitment of the centromere-specific histone H3 variant CENP-A. This process is fundamental for the assembly of the kinetochore and for ensuring proper chromosome segregation during cell division. Therefore, it is plausible that [MIS18A AS1](/details-gene/100874202) is involved in modulating the cell cycle, particularly the fidelity of mitosis, within the specific cellular context of [neural progenitor cell](/details-cell/CL0011020). ## Research Directions The specific expression of [MIS18A AS1](/details-gene/100874202) in [neural progenitor cell](/details-cell/CL0011020) raises important questions about its role in nervous system development and related pathologies. ### Testable Hypotheses 1. [MIS18A AS1](/details-gene/100874202) acts as a negative regulator of MIS18A expression in [neural progenitor cell](/details-cell/CL0011020) to precisely control the rate of cell division and prevent chromosomal instability during rapid proliferation. 2. The expression of [MIS18A AS1](/details-gene/100874202) is required to maintain the undifferentiated, self-renewing state of [neural progenitor cell](/details-cell/CL0011020), and its downregulation is a prerequisite for their terminal differentiation into mature neurons or glia. ### Proposed Experiments To test the hypothesis that [MIS18A AS1](/details-gene/100874202) regulates its sense transcript MIS18A, one could perform a loss-of-function study in a human neural progenitor cell line. Knockdown of [MIS18A AS1](/details-gene/100874202) could be achieved using antisense oligonucleotides (ASOs) or CRISPR interference (CRISPRi). The direct impact on MIS18A would be quantified at the mRNA and protein levels using RT-qPCR and western blotting, respectively. Downstream functional consequences could be assessed by measuring cell proliferation rates (e.g., via EdU incorporation assays) and examining chromosomal segregation fidelity (e.g., by microscopy for anaphase bridges or micronuclei formation). ### Therapeutic Potential As a non-coding RNA with a highly restricted expression pattern, [MIS18A AS1](/details-gene/100874202) presents an attractive profile for therapeutic development. Its specificity for [neural progenitor cell](/details-cell/CL0011020) could be exploited for targeted therapies against diseases involving the dysregulation of these cells, such as certain developmental brain disorders or cancers like medulloblastoma. Antisense oligonucleotides could be designed to inhibit its function if it is found to be pathogenic. Furthermore, its specificity makes it a potential biomarker for isolating or monitoring these progenitor populations in both research and clinical settings.