Details for: IL1R1 AS1

Gene ID: 112577460

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: IL1R1 AS1

Ensembl ID: ENSG00000226925

Description: IL1R1 antisense RNA 1

Cell Significance Landscape

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • hepatic stellate cell CL0000632
    CSI 4.55
    rCSI 17.06%
    PRS 100

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary Analyzed for its expression specificity (CSI Z-SCORE), the long non-coding RNA [IL1R1 AS1](/details-gene/112577460) is identified as a potentially highly specific marker for [hepatic stellate cells](/details-cell/CL0000632). Its name suggests a regulatory role related to the Interleukin 1 Receptor Type 1 ([IL1R1](/details-gene/3554)), a key component of inflammatory signaling. While its maximal effect size indicates exclusive expression in this cell type, the low statistical significance warrants further investigation to confirm its role. ## Cellular Roles and Expression Landscape The primary role of [IL1R1 AS1](/details-gene/112577460), as suggested by this specificity-focused analysis, is as a unique molecular identifier for [hepatic stellate cells](/details-cell/CL0000632). In the **Overall** tissue context, the data presents a compelling, albeit statistically nuanced, picture of its cell-type exclusivity. The gene achieves a perfect Effect Size (deltaVal: 1.00) and a top-tier percentile rank (PRS: 100.00%), which strongly suggests that its expression is tightly restricted to [hepatic stellate cells](/details-cell/CL0000632) compared to all other cell types in the surveyed environment. However, this high specificity is accompanied by a CSI (Z-SCORE) of 0.00 and a non-significant p-value (0.640). This combination may indicate that while [IL1R1 AS1](/details-gene/112577460) is exclusively expressed in this cell type, its transcript levels may be low or detected in a sparse subset of cells, impacting statistical power in this analysis. Given its antisense orientation to the [IL1R1](/details-gene/3554) gene, its presence in hepatic stellate cells points towards a potential role in regulating the inflammatory response within the liver microenvironment. Hepatic stellate cells are central players in liver fibrosis and inflammation, and precise control of inflammatory receptors like IL1R1 is critical to their function. The highly specific expression of this antisense transcript suggests it could be a key local modulator of IL-1 signaling, specific to this cell lineage. ## Pathways and Molecular Function Direct functional annotations for [IL1R1 AS1](/details-gene/112577460) are not available in this dataset. However, its genomic location and nomenclature as an antisense RNA to the Interleukin 1 Receptor Type 1 gene ([IL1R1](/details-gene/3554)) provide strong inferential evidence for its molecular function. [IL1R1](/details-gene/3554) is the primary signaling receptor for the pro-inflammatory cytokines IL-1-alpha and IL-1-beta. Its activation is a critical upstream event in canonical inflammatory cascades, including the MyD88-dependent pathway that leads to NF-kappaB activation. Antisense non-coding RNAs can regulate their sense-strand counterparts through various mechanisms, including transcriptional interference, mRNA masking, or directing epigenetic modifications. It is therefore highly probable that [IL1R1 AS1](/details-gene/112577460) functions to modulate the expression, stability, or translation of [IL1R1](/details-gene/3554) mRNA. This positions [IL1R1 AS1](/details-gene/112577460) as a putative regulator within pathways such as: * Interleukin-1 signaling * NF-kappaB signaling pathway * Cellular responses to cytokine stimulus The cell-specific expression in [hepatic stellate cells](/details-cell/CL0000632) suggests this regulation is a specialized mechanism for controlling inflammation and fibrogenesis in the liver. ## Research Directions The highly specific expression of [IL1R1 AS1](/details-gene/112577460) in hepatic stellate cells, coupled with its potential to regulate the critical inflammatory receptor [IL1R1](/details-gene/3554), opens several avenues for future research. Understanding its function could provide novel insights into the molecular control of liver fibrosis and inflammation. **Testable Hypotheses:** 1. **Hypothesis:** [IL1R1 AS1](/details-gene/112577460) acts as a negative regulator of [IL1R1](/details-gene/3554) expression, thereby dampening the pro-inflammatory and pro-fibrotic response of hepatic stellate cells to IL-1 stimulation. * **Experimental Approach:** Utilize antisense oligonucleotides (ASOs) or siRNA to specifically knock down [IL1R1 AS1](/details-gene/112577460) in primary human hepatic stellate cells or a relevant cell line (e.g., LX-2). Subsequently, treat the cells with IL-1beta and quantify changes in [IL1R1](/details-gene/3554) mRNA and protein levels via qPCR and western blotting. Functional consequences can be measured by assessing downstream NF-kappaB pathway activation and the expression of fibrotic markers like *ACTA2* and *COL1A1*. 2. **Hypothesis:** The expression of [IL1R1 AS1](/details-gene/112577460) is dynamically regulated during the transition of quiescent hepatic stellate cells to their activated, myofibroblast-like state in the context of liver injury. * **Experimental Approach:** Analyze existing or newly generated single-cell RNA-sequencing (scRNA-seq) datasets from human or mouse models of liver fibrosis (e.g., carbon tetrachloride or bile duct ligation models). Specifically track the expression of [IL1R1 AS1](/details-gene/112577460) and [IL1R1](/details-gene/3554) along the pseudo-temporal trajectory of hepatic stellate cell activation to determine if its levels change in a manner consistent with a regulatory role. 3. **Hypothesis:** [IL1R1 AS1](/details-gene/112577460) exerts its regulatory function by directly binding to the [IL1R1](/details-gene/3554) pre-mRNA, altering its splicing or stability, or by recruiting chromatin-modifying complexes to the [IL1R1](/details-gene/3554) promoter. * **Experimental Approach:** Perform RNA-RNA interaction assays like CHART (Capture Hybridization Analysis of RNA Targets) or ChIRP (Chromatin Isolation by RNA Purification) in hepatic stellate cells to confirm direct interaction with [IL1R1](/details-gene/3554) transcripts or its genomic locus. This could be followed by identifying interacting proteins via mass spectrometry to elucidate the specific molecular machinery involved. **Therapeutic Potential:** Given the central role of activated hepatic stellate cells in driving liver fibrosis, a major cause of morbidity and mortality worldwide, molecules that can selectively modulate their behavior are of high therapeutic interest. The exquisite cell-type specificity of [IL1R1 AS1](/details-gene/112577460) makes it an attractive therapeutic target. ASO-based therapies designed to upregulate [IL1R1 AS1](/details-gene/112577460) could potentially be used to suppress [IL1R1](/details-gene/3554)-mediated inflammation and activation in hepatic stellate cells, offering a targeted approach to treating fibrotic liver diseases such as non-alcoholic steatohepatitis (NASH).