Details for: ZNF295 AS1

Gene ID: 150142

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: ZNF295 AS1

Ensembl ID: ENSG00000237232

Description: ZNF295 antisense RNA 1

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • ciliated epithelial cell CL0000067
    CSI 9.04
    rCSI 7.95%
    PRS 98.83
  • ciliated cell CL0000064
    CSI 4.05
    rCSI 6.56%
    PRS 98.77
  • multi-ciliated epithelial cell CL0005012
    CSI 3.23
    rCSI 3.22%
    PRS 99.17
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 1.42
    rCSI 3.23%
    PRS 98.78

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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    • High
    • Medium
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    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF295-AS1](/details-gene/150142) is a long non-coding RNA (lncRNA) gene located on chromosome 21. **Overall**, expression data strongly identifies this gene as a highly specific marker for ciliated cell lineages. Its significance is most pronounced in [ciliated epithelial cells](/details-cell/CL0000067), where it shows a Cell Significance Index (CSI) of 9.04, suggesting a fundamental role in the identity or function of these specialized cells. ## Cellular Roles and Expression Landscape The expression profile of [ZNF295-AS1](/details-gene/150142) is characterized by its remarkable specificity for ciliated cells. The gene demonstrates its highest significance in [ciliated epithelial cell](/details-cell/CL0000067), [ciliated cell](/details-cell/CL0000064), [multi-ciliated epithelial cell](/details-cell/CL0005012), and [ciliated columnar cell of tracheobronchial tree](/details-cell/CL0002145). This consistent and high-level expression pattern across various ciliated cell subtypes suggests that [ZNF295-AS1](/details-gene/150142) is likely involved in core biological processes essential to these cells, such as their differentiation, maturation, or the maintenance of ciliary function. The lack of significant expression in other major cell lineages (data not shown) underscores its potential as a highly specific biomarker for this cellular identity. ## Pathways and Molecular Function The functional annotation for [ZNF295-AS1](/details-gene/150142) is not specified in the provided data. However, based on its exclusive and high expression in ciliated cells, it is plausible to infer its involvement in pathways related to ciliogenesis, ciliary motility, or the regulation of epithelial cell differentiation programs. As a non-coding RNA, it may function as a molecular scaffold, a guide for chromatin-modifying complexes, or a sponge for microRNAs to regulate the expression of key proteins involved in these processes. ## Research Directions The highly specific expression pattern of [ZNF295-AS1](/details-gene/150142) in ciliated cells presents several avenues for future investigation. **Proposed Hypotheses:** 1. [ZNF295-AS1](/details-gene/150142) is a critical regulator of ciliogenesis, and its depletion impairs the formation and function of cilia in respiratory epithelial cells. 2. Given its location on chromosome 21, aberrant expression of [ZNF295-AS1](/details-gene/150142) may contribute to the upper respiratory tract pathologies or ciliary defects sometimes observed in individuals with Trisomy 21 (Down syndrome). **Suggested Experimental Approach:** To test the hypothesis that [ZNF295-AS1](/details-gene/150142) is essential for ciliogenesis, a loss-of-function study could be performed using an *in vitro* air-liquid interface (ALI) culture system with primary human bronchial epithelial cells. These cells can be transfected with antisense oligonucleotides (ASOs) or a CRISPRi system designed to specifically degrade or repress [ZNF295-AS1](/details-gene/150142) during their differentiation. The resulting cellular phenotype could be analyzed via immunofluorescence microscopy for ciliary markers (e.g., acetylated α-tubulin), scanning electron microscopy to assess ciliary structure, and high-speed video microscopy to measure ciliary beat frequency. **Therapeutic Potential:** As a non-coding RNA with a highly restricted expression pattern, [ZNF295-AS1](/details-gene/150142) could be an attractive therapeutic target. In diseases characterized by ciliary dysfunction (ciliopathies) or mucus clearance defects, such as chronic obstructive pulmonary disease (COPD) or primary ciliary dyskinesia, modulating its activity could be beneficial. Its specificity to ciliated cells suggests that RNA-based therapeutics (e.g., ASOs) targeting this gene might have a favorable safety profile with minimal off-target effects in other tissues. Therapeutic intervention would likely aim to restore its normal function in cases of deficiency or inhibit its activity if it is found to be pathologically overexpressed.