Details for: LTB4R2

Gene ID: 56413

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: LTB4R2

Ensembl ID: ENSG00000213906

Description: leukotriene B4 receptor 2

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • basal cell of epidermis CL0002187
    CSI 3.42
    rCSI 6.06%
    PRS 97.73
  • CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203
    CSI 2.26
    rCSI 2.74%
    PRS 98.42

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [LTB4R2](/details-gene/56413) (leukotriene B4 receptor 2) encodes a G protein-coupled receptor (GPCR) that functions as a low-affinity receptor for leukotriene B4, a potent lipid mediator of inflammation. As suggested by its functional annotations, [LTB4R2](/details-gene/56413) is involved in critical biological processes including [chemotaxis](/details-ontology/GO:0006935) and leukotriene signaling. The initial cloning and characterization of this receptor were described in several key studies ([Link](https://doi.org/10.1006/bbrc.2000.3152), [Link](https://doi.org/10.1074/jbc.c000382200), [Link](https://doi.org/10.1074/jbc.m004512200)). **Overall**, expression data indicates its most significant roles are in epithelial and immune cell populations, with particularly high significance in [basal cell of epidermis](/details-cell/CL0002187) and [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203). ## Cellular Roles and Expression Landscape The expression profile of [LTB4R2](/details-gene/56413) points to a dual function in both tissue barrier integrity and adaptive immunity. In the **Overall** context, [LTB4R2](/details-gene/56413) shows the highest significance in [basal cell of epidermis](/details-cell/CL0002187) (CSI: 3.42). This suggests a primary role in skin homeostasis and response to injury, which is consistent with its annotated function in [keratinocyte migration](/details-ontology/GO:0051546). Its activity in this progenitor cell layer of the epidermis may be crucial for wound healing and maintaining the epithelial barrier. Concurrently, [LTB4R2](/details-gene/56413) is a significant gene in [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203) (CSI: 2.26). This expression pattern suggests that [LTB4R2](/details-gene/56413) may mediate the trafficking and function of memory T cells, guiding them to sites of inflammation or reinfection in response to leukotriene B4 gradients. The absence of data on cell types with low expression limits a complete definition of its tissue specificity. ## Pathways and Molecular Function [LTB4R2](/details-gene/56413) functions as a [leukotriene b4 receptor activity](/details-ontology/GO:0001632), a member of the rhodopsin-like GPCR superfamily ([R-HSA-373076](https://reactome.org/content/detail/R-HSA-373076)). Upon binding its ligand, it initiates intracellular signaling cascades, primarily through [G alpha (q) signalling events](/details-pathway/R-HSA-416476). This signaling is integral to the broader [leukotriene signaling pathway](/details-ontology/GO:0061737), which plays a pivotal role in orchestrating inflammatory responses. The receptor's involvement in [chemotaxis](/details-ontology/GO:0006935) directly explains its significance in motile immune cells like [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), enabling them to migrate along inflammatory gradients. In parallel, its contribution to [keratinocyte migration](/details-ontology/GO:0051546) is consistent with its high expression in the [basal cell of epidermis](/details-cell/CL0002187), facilitating the re-epithelialization process during skin repair. The receptor is localized to the [plasma membrane](/details-ontology/GO:0005886), optimally positioned to detect extracellular lipid mediators. ## Research Directions The dual expression of [LTB4R2](/details-gene/56413) in both epithelial and immune cells suggests it acts as a key communication node during inflammation and tissue repair, making it a subject of significant scientific interest. One early study proposed it as a potential therapeutic target in inflammatory and immunological disorders ([Link](https://doi.org/10.1084/jem.192.3.421)). ### Proposed Hypotheses 1. **Hypothesis:** [LTB4R2](/details-gene/56413) signaling in epidermal basal cells is a critical rate-limiting step in wound healing, and its dysregulation contributes to chronic wound pathologies or inflammatory skin diseases like psoriasis. 2. **Hypothesis:** In [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), [LTB4R2](/details-gene/56413) is essential for the efficient recall response, mediating their rapid migration from circulation into peripheral tissues during a secondary antigen encounter. ### Experimental Approach To test the hypothesis regarding the role of [LTB4R2](/details-gene/56413) in memory T cell trafficking (Hypothesis 2), one could perform an adoptive transfer experiment. [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203) could be isolated from a human donor, subjected to CRISPR-Cas9-mediated knockout of [LTB4R2](/details-gene/56413) or a control guide RNA, and subsequently transferred into an immunodeficient mouse model bearing a human skin graft. Following an inflammatory challenge to the graft (e.g., with a recall antigen), the relative infiltration of knockout versus control T cells into the tissue could be quantified using flow cytometry or immunohistochemistry. A significant reduction in the infiltration of [LTB4R2](/details-gene/56413)-deficient cells would confirm its crucial role in memory T cell tissue homing. ### Therapeutic Potential As a cell-surface GPCR, [LTB4R2](/details-gene/56413) is a highly druggable target. Its involvement in pro-inflammatory pathways makes it a candidate for therapeutic **inhibition**. Small molecule antagonists targeting [LTB4R2](/details-gene/56413) could potentially dampen excessive inflammatory responses in a range of conditions, including autoimmune skin diseases (e.g., psoriasis), inflammatory bowel disease, or asthma. Given its expression on both epithelial and immune cells, such an inhibitor could have broad efficacy by simultaneously reducing immune cell infiltration and modulating the epithelial response to inflammation.

Genular Protein ID: 2486064227

Symbol: LT4R2_HUMAN

Name: Leukotriene B4 receptor 2

UniProtKB Accession Codes:

Q9NPC1 Q5KU28 Q9NPE5

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 ChEMBL 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 2 EMBL 11 Ensembl 4 ExpressionAtlas 1 GO 10 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 GuidetoPHARMACOLOGY 1 HGNC 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PIR 1 PRINTS 3 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 RNAct 1 Reactome 2 RefSeq 2 SIGNOR 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissLipids 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 10913346

Title: Cloning and characterization of cDNA encoding a novel human leukotriene B4 receptor.

PubMed ID: 10913346

DOI: 10.1006/bbrc.2000.3152

PubMed ID: 10889186

Title: Molecular cloning and characterization of another leukotriene B4 receptor.

PubMed ID: 10889186

DOI: 10.1074/jbc.c000382200

PubMed ID: 11006272

Title: A novel hepatointestinal leukotriene B4 receptor: cloning and functional characterization.

PubMed ID: 11006272

DOI: 10.1074/jbc.m004512200

PubMed ID: 10934230

Title: A second leukotriene B4 receptor, BLT2: a new therapeutic target in inflammation and immunological disorders.

PubMed ID: 10934230

DOI: 10.1084/jem.192.3.421

PubMed ID: 12044878

Title: Identification of G protein-coupled receptor genes from the human genome sequence.

PubMed ID: 12044878

DOI: 10.1016/s0014-5793(02)02775-8

PubMed ID: 12508121

Title: The DNA sequence and analysis of human chromosome 14.

PubMed ID: 12508121

DOI: 10.1038/nature01348

Sequence Information:

  • Length: 358
  • Mass: 37942
  • Checksum: 5F5D98179CE00F3D
  • Sequence:
    • MSVCYRPPGN ETLLSWKTSR ATGTAFLLLA ALLGLPGNGF VVWSLAGWRP ARGRPLAATL 
VLHLALADGA VLLLTPLFVA FLTRQAWPLG QAGCKAVYYV CALSMYASVL LTGLLSLQRC 
LAVTRPFLAP RLRSPALARR LLLAVWLAAL LLAVPAAVYR HLWRDRVCQL CHPSPVHAAA 
HLSLETLTAF VLPFGLMLGC YSVTLARLRG ARWGSGRHGA RVGRLVSAIV LAFGLLWAPY 
HAVNLLQAVA ALAPPEGALA KLGGAGQAAR AGTTALAFFS SSVNPVLYVF TAGDLLPRAG 
PRFLTRLFEG SGEARGGGRS REGTMELRTT PQLKVVGQGR GNGDPGGGME KDGPEWDL

Genular Protein ID: 953401591

Symbol: B4E292_HUMAN

Name: N/A

UniProtKB Accession Codes:

B4E292

Database IDs:

ARBA 9 AlphaFoldDB 1 BioGRID-ORCS 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 2 GO 2 Gene3D 1 InterPro 4 KEGG 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PRINTS 3 PROSITE 2 PeptideAtlas 1 Pfam 1 PharmGKB 1 RefSeq 2 SAM 2 SUPFAM 1

Sequence Information:

  • Length: 358
  • Mass: 37943
  • Checksum: 5F5D90BD3EE8A59F
  • Sequence:
    • MSVCYRPPGN ETLLSWKTSR ATGTAFLLLA ALLGLPGNGF VVWSLAGWRP ARGRPLAATL 
VLHLALADGA VLLLTPLFVA FLTRQAWPLG QAGCKAVYYV CALSMYASVL LTGLLSLQRC 
LAVTRPFLAP RLRSPALARR LLLAVWLAAL LLAVPAAVYR HLWRDRVCQL CHPSPVHAAA 
HLSLETLTAF VLPFGLMLGC YSVTLARLRG ARWGSGRHGA RVGRLVSAIV LAFGLLWAPY 
HAVNLLQAVA ALAPPEGALA KLGGAGQAAR AGTTALAFFS SSVDPVLYVF TAGDLLPRAG 
PRFLTRLFEG SGEARGGGRS REGTMELRTT PQLKVVGQGR GNGDPGGGME KDGPEWDL