Details for: PRSS27

Gene ID: 83886

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: PRSS27

Ensembl ID: ENSG00000172382

Description: serine protease 27

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • squamous epithelial cell CL0000076
    CSI 5.23
    rCSI 12.41%
    PRS 99.55
  • epithelial cell CL0000066
    CSI 4.34
    rCSI 6.67%
    PRS 99.32
  • epithelial cell of esophagus CL0002252
    CSI 1.07
    rCSI 10.6%
    PRS 99.72

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Node Color (Target Cell CSI, relative to current network):
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    • High
    • Medium
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    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [PRSS27](/details-gene/83886), or Serine Protease 27, is a protein-coding gene located on chromosome 16p13.3. It encodes a serine-type endopeptidase that is localized to the plasma membrane and extracellular region. The primary function of the [PRSS27](/details-gene/83886) protein is proteolysis. Expression data indicate that [PRSS27](/details-gene/83886) is a highly significant marker for epithelial lineages, particularly [squamous epithelial cell](/details-cell/CL0000076) and the [epithelial cell of esophagus](/details-cell/CL0002252), suggesting a specialized role in the maintenance, remodeling, and barrier function of stratified epithelial tissues. ## Cellular Roles and Expression Landscape The expression profile of [PRSS27](/details-gene/83886) is strongly concentrated within epithelial cell types, establishing it as a key component of these tissues. **Overall**, the gene demonstrates its highest significance in [squamous epithelial cell](/details-cell/CL0000076) (CSI: 5.23), followed by a broader significance in the general [epithelial cell](/details-cell/CL0000066) population (CSI: 4.34). Its specific expression in [epithelial cell of esophagus](/details-cell/CL0002252) (CSI: 1.07) further supports a role in specialized stratified epithelia that form protective barriers. This restricted expression pattern suggests that its proteolytic activity is likely tailored to the unique physiological demands of these tissues, such as cell turnover and maintenance of the extracellular environment. ## Pathways and Molecular Function [PRSS27](/details-gene/83886) is functionally annotated as a serine-type endopeptidase involved in proteolysis ([GO:0006508](https://www.ebi.ac.uk/QuickGO/term/GO:0006508), [GO:0004252](https://www.ebi.ac.uk/QuickGO/term/GO:0004252)). Its localization to the extracellular region and plasma membrane ([GO:0005576](https://www.ebi.ac.uk/QuickGO/term/GO:0005576), [GO:0005886](https://www.ebi.ac.uk/QuickGO/term/GO:0005886)) is consistent with its discovery as a novel secreted or transmembrane protein ([Link](https://doi.org/10.1101/gr.1293003)). This positioning enables it to act on extracellular matrix proteins or cell-surface molecules, a function critical for tissue homeostasis in dynamic epithelial layers. An early study also referred to this protease as "pancreasin," noting its expression in the pancreas, an organ rich in secretory epithelial cells ([Link](https://doi.org/10.1074/jbc.m209353200)). This finding, combined with its high significance in other epithelia, highlights its importance in tissues with high secretory and turnover rates. ## Research Directions Based on its specific expression and enzymatic function, the role of [PRSS27](/details-gene/83886) in both physiological and pathological processes warrants further investigation. **Proposed Testable Hypotheses:** 1. Given its high expression in [squamous epithelial cell](/details-cell/CL0000076) and its extracellular proteolytic activity, [PRSS27](/details-gene/83886) may be a key regulator of desquamation (the shedding of terminally differentiated keratinocytes) by cleaving cell-cell adhesion molecules in the outermost epidermal layers. 2. Aberrant expression or activity of [PRSS27](/details-gene/83886) in esophageal tissue could be a contributing factor to the pathogenesis of esophageal squamous cell carcinoma, potentially by promoting degradation of the basement membrane and facilitating tumor cell invasion. **Suggested Experimental Approach:** To test the hypothesis that [PRSS27](/details-gene/83886) regulates epithelial desquamation, a 3D organoid model of human stratified epithelium could be employed. Using CRISPR-Cas9 to knock out [PRSS27](/details-gene/83886) in primary keratinocytes, one could then assess the impact on the organoid's structure and turnover. The rate of shedding of the superficial cell layers could be quantified via live-cell imaging, and immunofluorescence staining for key adhesion molecules (e.g., Desmoglein-1, E-cadherin) at different stages of differentiation would reveal defects in cell-cell junction disassembly. **Therapeutic Potential:** As an extracellular and membrane-associated protease, [PRSS27](/details-gene/83886) represents a highly accessible therapeutic target. If its activity is shown to be elevated in epithelial-derived cancers and contributes to disease progression, it would be a prime candidate for targeted **inhibition**. The development of specific small molecule inhibitors or therapeutic antibodies could block its proteolytic activity, potentially reducing tumor invasion and metastasis. The gene's relatively restricted expression in specific epithelial tissues suggests that such an approach might have a favorable safety profile with limited off-target effects.

Genular Protein ID: 65991231

Symbol: PRS27_HUMAN

Name: Serine protease 27

UniProtKB Accession Codes:

Q9BQR3

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EC 1 EMBL 5 Ensembl 1 ExpressionAtlas 1 GO 4 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 KEGG 1 MANE-Select 1 MEROPS 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 RefSeq 2 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 12441343

Title: Structure and activity of human pancreasin, a novel tryptic serine peptidase expressed primarily by the pancreas.

PubMed ID: 12441343

DOI: 10.1074/jbc.m209353200

PubMed ID: 12975309

Title: The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.

PubMed ID: 12975309

DOI: 10.1101/gr.1293003

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 290
  • Mass: 31940
  • Checksum: 67BDC93EC70BFF7B
  • Sequence:
    • MRRPAAVPLL LLLCFGSQRA KAATACGRPR MLNRMVGGQD TQEGEWPWQV SIQRNGSHFC 
GGSLIAEQWV LTAAHCFRNT SETSLYQVLL GARQLVQPGP HAMYARVRQV ESNPLYQGTA 
SSADVALVEL EAPVPFTNYI LPVCLPDPSV IFETGMNCWV TGWGSPSEED LLPEPRILQK 
LAVPIIDTPK CNLLYSKDTE FGYQPKTIKN DMLCAGFEEG KKDACKGDSG GPLVCLVGQS 
WLQAGVISWG EGCARQNRPG VYIRVTAHHN WIHRIIPKLQ FQPARLGGQK