Details for: C6orf58

Gene ID: 352999

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: C6orf58

Ensembl ID: ENSG00000184530

Description: chromosome 6 open reading frame 58

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • mucus secreting cell CL0000319
    CSI 4.98
    rCSI 7.91%
    PRS 99.91
  • mucous neck cell CL0000651
    CSI 4.06
    rCSI 5.85%
    PRS 99.84
  • enteroendocrine cell CL0000164
    CSI 3.37
    rCSI 4.61%
    PRS 99.63
  • serous secreting cell CL0000313
    CSI 2.97
    rCSI 15.01%
    PRS 99.91
  • tracheobronchial serous cell CL0019001
    CSI 2.18
    rCSI 9.4%
    PRS 99.9
  • serous secreting cell of bronchus submucosal gland CL4033005
    CSI 1.24
    rCSI 7.07%
    PRS 99.58

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [C6orf58](/details-gene/352999) is a protein-coding gene located on human chromosome 6. While its function is not yet fully characterized, available data strongly suggest it encodes a secreted protein. Functional annotations indicate its presence in the [extracellular space (GO:0005615)](https://www.ebi.ac.uk/QuickGO/term/GO:0005615) and within [extracellular exosomes (GO:0070062)](https://www.ebi.ac.uk/QuickGO/term/GO:0070062). This is highly consistent with its expression profile, which shows significant and specific enrichment in various secretory cell types, including [mucus secreting cells](/details-cell/CL0000319) and [serous secreting cells](/details-cell/CL0000313). Proteomic studies have experimentally confirmed the presence of the [C6orf58](/details-gene/352999) protein in human saliva ([Link](https://doi.org/10.1021/pr050492k)), underscoring its role as a secreted component of mucosal surfaces. ## Cellular Roles and Expression Landscape The expression pattern of [C6orf58](/details-gene/352999) points to a specialized role in glandular and mucosal tissues. **Overall**, the gene shows its highest significance in a range of secretory epithelial cells. It is a top marker for [mucus secreting cells](/details-cell/CL0000319) (CSI: 4.98) and [mucous neck cells](/details-cell/CL0000651) (CSI: 4.06) of the gastrointestinal tract, as well as [enteroendocrine cells](/details-cell/CL0000164) (CSI: 3.37). Furthermore, its high significance extends to cells responsible for producing serous fluid, such as general [serous secreting cells](/details-cell/CL0000313) (CSI: 2.97) and more specialized types like [tracheobronchial serous cells](/details-cell/CL0019001) (CSI: 2.18) and [serous secreting cells of the bronchus submucosal gland](/details-cell/CL4033005) (CSI: 1.24). This highly specific expression landscape, concentrated in cells that secrete substances to line and protect epithelial surfaces, strongly suggests that [C6orf58](/details-gene/352999) is involved in the biology of mucosal immunity and barrier function. ## Pathways and Molecular Function The known functional annotations for [C6orf58](/details-gene/352999) align directly with its observed cellular expression. The gene product is localized to the [extracellular space (GO:0005615)](https://www.ebi.ac.uk/QuickGO/term/GO:0005615) and is found in [extracellular exosomes (GO:0070062)](https://www.ebi.ac.uk/QuickGO/term/GO:0070062), which is consistent with its high expression in cells specialized for secretion. The identification of the protein in human saliva provides direct experimental support for this secreted nature ([Link](https://doi.org/10.1021/pr050492k)). Its annotated molecular function of [protein binding (GO:0005515)](https://www.ebi.ac.uk/QuickGO/term/GO:0005515) is general but suggests that once secreted, the [C6orf58](/details-gene/352999) protein likely interacts with other extracellular or cell-surface proteins to exert its function, perhaps as a component of the mucus layer or saliva. ## Research Directions The specific expression pattern and secreted nature of [C6orf58](/details-gene/352999) suggest several avenues for future research into its role in mucosal biology and pathology. **Proposed Hypotheses:** 1. Given its high expression in [mucus secreting cells](/details-cell/CL0000319) and its protein-binding capacity, [C6orf58](/details-gene/352999) may function as a structural or regulatory component of the mucus barrier, potentially by cross-linking mucin polymers or interacting with other secreted proteins to modulate mucus viscosity and integrity. 2. Based on its confirmed presence in saliva ([Link](https://doi.org/10.1021/pr050492k)) and in the enamel proteome ([Link](https://doi.org/10.1177/0022034510375824)), the [C6orf58](/details-gene/352999) protein may play a role in the oral cavity, such as contributing to the antimicrobial properties of saliva or participating in the maintenance of tooth enamel. **Experimental Approach:** To test the hypothesis that [C6orf58](/details-gene/352999) is involved in mucosal barrier function (Hypothesis 1), one could utilize an *in vitro* air-liquid interface (ALI) culture system with primary human bronchial epithelial cells, which differentiate to form a mucus-producing epithelium. [C6orf58](/details-gene/352999) expression could be silenced using siRNA or knocked out via CRISPR-Cas9. The resulting impact on the mucosal barrier could be assessed by measuring changes in mucin (e.g., MUC5AC) secretion via ELISA, evaluating mucus rheology, and testing barrier integrity using transepithelial electrical resistance (TEER) measurements. Additionally, co-immunoprecipitation followed by mass spectrometry on the secreted protein could identify its binding partners within the mucus layer. **Therapeutic Potential:** The therapeutic potential of [C6orf58](/details-gene/352999) is currently speculative but intriguing. As a secreted protein with a highly restricted expression pattern, it represents an excellent candidate as a non-invasive biomarker. Altered levels of the [C6orf58](/details-gene/352999) protein in saliva, sputum, or serum could potentially signal pathologies affecting secretory tissues, such as Sjögren's syndrome, cystic fibrosis, or certain cancers. If the protein is shown to have a critical role in mucosal defense, recombinant [C6orf58](/details-gene/352999) could be explored as a therapeutic agent to bolster compromised mucosal barriers in respiratory or gastrointestinal diseases.

Genular Protein ID: 730283121

Symbol: LEG1H_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q6P5S2 B4E1I0 Q5VUP2

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 4 Ensembl 1 GO 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 1 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRIDE 1 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 RefSeq 1 SMR 1 STRING 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 16740002

Title: Identification of N-linked glycoproteins in human saliva by glycoprotein capture and mass spectrometry.

PubMed ID: 16740002

DOI: 10.1021/pr050492k

PubMed ID: 20651090

Title: Surface integrity governs the proteome of hypomineralized enamel.

PubMed ID: 20651090

DOI: 10.1177/0022034510375824

Sequence Information:

  • Length: 330
  • Mass: 37926
  • Checksum: 75F8219FC2C76914
  • Sequence:
    • MAFLPSWVCV LVGSFSASLA GTSNLSETEP PLWKESPGQL SDYRVENSMY IINPWVYLER 
MGMYKIILNQ TARYFAKFAP DNEQNILWGL PLQYGWQYRT GRLADPTRRT NCGYESGDHM 
CISVDSWWAD LNYFLSSLPF LAAVDSGVMG ISSDQVRLLP PPKNERKFCY DVSSCRSSFP 
ETMNKWNTFY QYLQSPFSKF DDLLKYLWAA HTSTLADNIK SFEDRYDYYS KAEAHFERSW 
VLAVDHLAAV LFPTTLIRSY KFQKGMPPRI LLNTDVAPFI SDFTAFQNVV LVLLNMLDNV 
DKSIGYLCTE KSNVYRDHSE SSSRSYGNNS