Details for: NSMCE1 DT

Gene ID: 400512

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: NSMCE1 DT

Ensembl ID: ENSG00000245888

Description: NSMCE1 divergent transcript

Cell Significance Landscape

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • granulocyte CL0000094
    CSI 3.11
    rCSI 4.75%
    PRS 99.83
  • basophil CL0000767
    CSI 2.67
    rCSI 5.64%
    PRS 99.85

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
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  • Node Color (Target Cell CSI, relative to current network):
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    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary `[NSMCE1 DT](/details-gene/400512)` is a non-coding RNA (ncRNA) located on chromosome 16p12.1. Gene expression data indicate that its role is particularly significant within the myeloid lineage of the innate immune system. Specifically, it shows high expression significance in `[granulocytes](/details-cell/CL0000094)` and `[basophils](/details-cell/CL0000767)`, suggesting a potential function in the development or activity of these cell types. As a divergent transcript, it is transcribed in the opposite direction from the neighboring protein-coding gene *NSMCE1*, which may imply a regulatory relationship. ## Cellular Roles and Expression Landscape The expression profile of `[NSMCE1 DT](/details-gene/400512)` points towards a specialized role in innate immunity. **Overall**, the gene's significance is most pronounced in terminally differentiated myeloid cells. The highest significance is observed in: * `[granulocyte](/details-cell/CL0000094)` (CSI: 3.11) * `[basophil](/details-cell/CL0000767)` (CSI: 2.67) This restricted expression pattern suggests that `[NSMCE1 DT](/details-gene/400512)` is not a broadly expressed regulatory molecule but may be involved in specific processes unique to these phagocytic and allergic-response effector cells, such as their maturation, activation, or the execution of their inflammatory functions. ## Pathways and Molecular Function Functional annotation data for `[NSMCE1 DT](/details-gene/400512)` is not currently available. As a non-coding RNA, its function is presumed to be regulatory. The designation 'divergent transcript' indicates its transcription initiates near and proceeds in the opposite direction to the protein-coding gene `[NSMCE1](/details-gene/55114)`, a core component of the SMC5/6 complex involved in DNA repair and maintaining chromosome stability. This genomic arrangement raises the possibility of a *cis*-regulatory relationship, where `[NSMCE1 DT](/details-gene/400512)` could modulate the expression or function of `[NSMCE1](/details-gene/55114)` in the specific cellular contexts of granulocytes and basophils. ## Research Directions The specific expression of `[NSMCE1 DT](/details-gene/400512)` in myeloid cells offers several avenues for future investigation. Its function as an ncRNA remains uncharacterized, presenting an opportunity to uncover novel regulatory mechanisms in innate immunity. **Testable Hypotheses:** 1. `[NSMCE1 DT](/details-gene/400512)` is a critical regulator of granulopoiesis, with its expression required for the terminal differentiation of myeloid progenitors into mature `[granulocytes](/details-cell/CL0000094)`. 2. The ncRNA acts as a local modulator of the adjacent `[NSMCE1](/details-gene/55114)` gene, influencing DNA damage responses or genome stability specifically within the granulocytic lineage. 3. In `[basophils](/details-cell/CL0000767)`, `[NSMCE1 DT](/details-gene/400512)` is involved in regulating the expression of genes essential for degranulation and the allergic inflammatory response. **Proposed Experimental Approach:** To test the hypothesis that `[NSMCE1 DT](/details-gene/400512)` is essential for granulocyte differentiation, one could utilize an *in vitro* model of human hematopoiesis. Specifically, human CD34+ hematopoietic stem and progenitor cells (HSPCs) could be cultured under conditions that promote granulocytic differentiation. The expression of `[NSMCE1 DT](/details-gene/400512)` would be specifically suppressed using locked nucleic acid (LNA) GapmeRs or an shRNA-based approach. The impact of its knockdown on differentiation would be monitored over time by flow cytometry for key surface markers (e.g., CD11b, CD15, CD16) and by morphological analysis of cells. A corresponding change in the expression of key myeloid transcription factors (e.g., C/EBPα, PU.1) could be quantified via RT-qPCR to dissect the molecular mechanism. **Therapeutic Potential:** Given its restricted expression in myeloid cells, `[NSMCE1 DT](/details-gene/400512)` could represent a highly specific therapeutic target. In diseases characterized by excessive granulocyte or basophil activity, such as chronic myeloid leukemia (CML) or certain severe allergic disorders, targeting this ncRNA could offer a novel strategy. Inhibition, likely via antisense oligonucleotides (ASOs), could potentially dampen the inflammatory contribution of these cells or impair the survival of malignant myeloid clones with minimal off-target effects on other tissues. However, its function must be elucidated before its therapeutic potential can be fully assessed.