Details for: CD300LG

Gene ID: 146894

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CD300LG

Ensembl ID: ENSG00000161649

Description: CD300 molecule like family member g

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • melanocyte of skin CL1000458
    CSI 8.62
    rCSI 11.74%
    PRS 94.74
  • capillary endothelial cell CL0002144
    CSI 3.6
    rCSI 6.6%
    PRS 99.88
  • blood vessel endothelial cell CL0000071
    CSI 3.54
    rCSI 7.34%
    PRS 99.69
  • cardiac endothelial cell CL0010008
    CSI 2.84
    rCSI 11.46%
    PRS 99.86
  • endothelial cell of vascular tree CL0002139
    CSI 1.12
    rCSI 6.13%
    PRS 98.84
  • vein endothelial cell of respiratory system CL4033008
    CSI 0.95
    rCSI 6.51%
    PRS 99.87
  • lung microvascular endothelial cell CL2000016
    CSI 0.6
    rCSI 11.53%
    PRS 99.94

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CD300LG](/details-gene/146894) (CD300 molecule like family member g) is a protein-coding gene located on chromosome 17q21.31. Functionally, it is classified as a transmembrane signaling receptor belonging to the immunoglobulin-like (Ig-like) CD300 family of proteins. Annotation data strongly suggest its involvement in immune regulation, particularly in mediating interactions between lymphoid and non-lymphoid cells. Expression analysis reveals that **Overall**, [CD300LG](/details-gene/146894) is a highly significant marker for endothelial cells across various tissues, with a particularly exclusive and pronounced significance in [capillary endothelial cells](/details-cell/CL0002144). Notably, its highest expression significance is observed in [melanocytes of skin](/details-cell/CL1000458), suggesting a specialized role in cutaneous biology. ## Cellular Roles and Expression Landscape The expression profile of [CD300LG](/details-gene/146894) points to a primary role within the vascular endothelium. **Overall**, the gene shows high significance in multiple endothelial cell populations, including [capillary endothelial cell](/details-cell/CL0002144) (CSI: 3.60), [blood vessel endothelial cell](/details-cell/CL0000071) (CSI: 3.54), [cardiac endothelial cell](/details-cell/CL0010008) (CSI: 2.84), and [lung microvascular endothelial cell](/details-cell/CL2000016) (CSI: 0.60). This consistent pattern, highlighted in a study that identified it as a novel protein exclusively expressed on capillary endothelium [Link](https://doi.org/10.1016/j.bbrc.2006.07.047), establishes [CD300LG](/details-gene/146894) as a key molecular feature of the endothelial lining, especially within the microvasculature. Beyond the endothelium, [CD300LG](/details-gene/146894) displays its most profound significance in [melanocyte of skin](/details-cell/CL1000458) (CSI: 8.62). This exceptionally high score suggests a distinct and potentially critical function in this cell type, which may be related to immune surveillance or pigmentation processes within the skin microenvironment. The co-expression in both endothelial cells and melanocytes suggests a potential role at the interface of the circulatory system and peripheral tissues. ## Pathways and Molecular Function The functional annotations for [CD300LG](/details-gene/146894) are consistent with its cellular expression profile. As a protein localized to the [plasma membrane](/details-cell/GO:0005886), including both [apical](/details-cell/GO:0016324) and [basolateral](/details-cell/GO:0016323) domains, it is well-positioned to act as a receptor. Its annotated molecular function as a [transmembrane signaling receptor activity](/details-cell/GO:0004888) supports a role in sensing the extracellular environment and initiating intracellular signals. Biologically, [CD300LG](/details-gene/146894) is implicated in the [immune system process](/details-cell/GO:0002376) and is a component of the [Adaptive immune system](/details-cell/R-HSA-1280218) pathway. More specifically, its involvement in [Immunoregulatory interactions between a lymphoid and a non-lymphoid cell](/details-cell/R-HSA-198933) suggests that its expression on non-lymphoid endothelial cells and melanocytes serves to directly modulate the activity of circulating or tissue-resident immune cells. This function is critical for processes such as leukocyte trafficking, immune surveillance, and maintaining tissue homeostasis. ## Research Directions The specific expression of [CD300LG](/details-gene/146894) on the endothelium and in melanocytes, combined with its immunoregulatory function, opens several avenues for future investigation. **Proposed Hypotheses:** 1. **Hypothesis 1:** [CD300LG](/details-gene/146894) on capillary endothelial cells functions as a receptor for a specific ligand on circulating T-lymphocytes or myeloid cells, regulating their adhesion and trans-endothelial migration into tissues during inflammation or immune surveillance. 2. **Hypothesis 2:** In the skin, [CD300LG](/details-gene/146894) expression on melanocytes mediates a direct signaling interaction with resident immune cells, such as Langerhans cells or dermal dendritic cells. This interaction could play a role in modulating the local immune response to pathogens or in the context of autoimmune skin disorders or melanoma. **Experimental Approach:** To test Hypothesis 1, a multi-step approach could be employed. First, a soluble [CD300LG](/details-gene/146894)-Fc fusion protein could be used as an affinity probe in flow cytometry to screen a panel of primary human leukocytes to identify the cell type(s) that express its counter-receptor. Once a binding partner is identified, co-immunoprecipitation from cell lysates followed by mass spectrometry could be used to determine its molecular identity. Functionally, an in-vitro trans-endothelial migration assay using human umbilical vein endothelial cells (HUVECs) could be performed. Knockdown of [CD300LG](/details-gene/146894) in HUVECs via siRNA or CRISPR-Cas9 would be expected to alter the migration rate of the identified leukocyte subset across the endothelial monolayer. **Therapeutic Potential:** As a cell surface protein with specific expression on the vascular endothelium, [CD300LG](/details-gene/146894) represents a potentially valuable therapeutic target. If it is found to promote inflammatory cell infiltration, a monoclonal antibody antagonist that blocks its function could be developed as a novel anti-inflammatory therapy for conditions characterized by excessive leukocyte recruitment, such as vasculitis or certain autoimmune diseases. Conversely, if its role is inhibitory, an agonist could be explored to suppress unwanted immune responses. Its high specificity for the endothelium may allow for targeted drug delivery and minimize off-target effects.

Genular Protein ID: 2049316541

Symbol: CLM9_HUMAN

Name: CMRF35-like molecule 9

UniProtKB Accession Codes:

Q6UXG3 B4DNY5 F5H7P9 F8W9M3 Q8IX38 Q8IX39 Q8TA95

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 4 CDD 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 8 Ensembl 5 ExpressionAtlas 1 GO 5 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 5 RNAct 1 Reactome 1 RefSeq 4 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 12975309

Title: The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.

PubMed ID: 12975309

DOI: 10.1101/gr.1293003

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16625196

Title: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

PubMed ID: 16625196

DOI: 10.1038/nature04689

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 15340161

Title: Signal peptide prediction based on analysis of experimentally verified cleavage sites.

PubMed ID: 15340161

DOI: 10.1110/ps.04682504

PubMed ID: 16876123

Title: CD300 antigen like family member G: a novel Ig receptor like protein exclusively expressed on capillary endothelium.

PubMed ID: 16876123

DOI: 10.1016/j.bbrc.2006.07.047

Sequence Information:

  • Length: 332
  • Mass: 36060
  • Checksum: 2347FF0FA55515FB
  • Sequence:
    • MRLLVLLWGC LLLPGYEALE GPEEISGFEG DTVSLQCTYR EELRDHRKYW CRKGGILFSR 
CSGTIYAEEE GQETMKGRVS IRDSRQELSL IVTLWNLTLQ DAGEYWCGVE KRGPDESLLI 
SLFVFPGPCC PPSPSPTFQP LATTRLQPKA KAQQTQPPGL TSPGLYPAAT TAKQGKTGAE 
APPLPGTSQY GHERTSQYTG TSPHPATSPP AGSSRPPMQL DSTSAEDTSP ALSSGSSKPR 
VSIPMVRILA PVLVLLSLLS AAGLIAFCSH LLLWRKEAQQ ATETQRNEKF CLSRLTAEEK 
EAPSQAPEGD VISMPPLHTS EEELGFSKFV SA