Details for: NXT2

Gene ID: 55916

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NXT2

Ensembl ID: ENSG00000101888

Description: nuclear transport factor 2 like export factor 2

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • epithelial cell of lung CL0000082
    CSI 3.6
    rCSI 2.98%
    PRS 98.67
  • neural progenitor cell CL0011020
    CSI 3.53
    rCSI 15.54%
    PRS 92.26
  • common myeloid progenitor CL0000049
    CSI 3.42
    rCSI 2.77%
    PRS 98.91
  • plasmacytoid dendritic cell, human CL0001058
    CSI 3.35
    rCSI 2.34%
    PRS 99.02
  • club cell CL0000158
    CSI 2.47
    rCSI 3.62%
    PRS 97
  • forebrain radial glial cell CL0013000
    CSI 1.16
    rCSI 3.71%
    PRS 97.81

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NXT2](/details-gene/55916), or Nuclear Transport Factor 2 Like Export Factor 2, is a protein-coding gene located on the X chromosome. It encodes the protein p15-2, which is implicated in the fundamental cellular process of transporting messenger RNA (mRNA) from the nucleus to the cytoplasm. Functional annotations strongly support its role as a component of the nuclear RNA export factor complex, essential for the transport of poly(A)+ mRNA through the nuclear pore. Expression data indicates that **Overall**, [NXT2](/details-gene/55916) is a significant gene in a diverse array of cell types, including [epithelial cell of lung](/details-cell/CL0000082), [neural progenitor cell](/details-cell/CL0011020), and [common myeloid progenitor](/details-cell/CL0000049), suggesting its function is critical in cells with high transcriptional activity and developmental potential. ## Cellular Roles and Expression Landscape The expression profile of [NXT2](/details-gene/55916) highlights its importance in functionally diverse cell populations, consistent with its core role in nuclear export, a process vital for all eukaryotic cells. **Overall**, it shows high significance in both progenitor and terminally differentiated cells. Its highest significance is observed in [epithelial cell of lung](/details-cell/CL0000082) (CSI: 3.60), suggesting a key role in maintaining the high metabolic and protein synthesis demands of this barrier tissue. Furthermore, its prominence in progenitor populations, such as [neural progenitor cell](/details-cell/CL0011020) (CSI: 3.53) and [common myeloid progenitor](/details-cell/CL0000049) (CSI: 3.42), underscores its likely importance in facilitating the robust gene expression programs required for cell division and differentiation. The high expression in [plasmacytoid dendritic cell, human](/details-cell/CL0001058) (CSI: 3.35) may reflect the need for rapid and large-scale export of cytokine and interferon mRNAs, a hallmark of this cell type's function in antiviral immunity. This broad but significant expression pattern across different lineages suggests [NXT2](/details-gene/55916) is a crucial component of the general cellular machinery rather than a specific lineage marker. ## Pathways and Molecular Function The function of [NXT2](/details-gene/55916) is centrally involved in nucleocytoplasmic transport. Gene Ontology annotations specify its role in the biological process of [mRNA export from nucleus](/details-molecular-function/GO:0006406), particularly [poly(A)+ mRNA export from nucleus](/details-molecular-function/GO:0016973). This is achieved through its molecular function of [protein binding](/details-molecular-function/GO:0005515) and its incorporation into the [nuclear RNA export factor complex](/details-cellular-component/GO:0042272). Cellular component annotations place the [NXT2](/details-gene/55916) protein in the [nucleus](/details-cellular-component/GO:0005634), [nucleoplasm](/details-cellular-component/GO:0005654), and [cytosol](/details-cellular-component/GO:0005829), and critically at the site of its function within the [nuclear pore central transport channel](/details-cellular-component/GO:0044613). This localization is consistent with its role in mediating the transit of macromolecules between the nucleus and cytoplasm. The high expression in progenitor cells and specialized secretory cells like [club cell](/details-cell/CL0000158) is directly aligned with these pathways, as these cells rely heavily on efficient gene expression, which is gated by the rate of mRNA export. ## Research Directions The widespread and significant expression of [NXT2](/details-gene/55916) in metabolically active and developing cells suggests it is a critical housekeeping gene. Research should focus on how its function is regulated and what cellular processes are most vulnerable to its disruption. **Testable Hypotheses:** 1. Disruption of [NXT2](/details-gene/55916) function will disproportionately impair the differentiation of progenitor cells, such as [common myeloid progenitor](/details-cell/CL0000049), by creating a bottleneck in the nuclear export of transcripts essential for lineage commitment. 2. In [plasmacytoid dendritic cell, human](/details-cell/CL0001058), [NXT2](/details-gene/55916) is essential for the rapid, high-volume export of Type I interferon mRNA following viral sensing, and its knockdown will selectively cripple this primary antiviral response. **Proposed Experiment:** To test the second hypothesis, primary human [plasmacytoid dendritic cell, human](/details-cell/CL0001058) could be isolated and treated with siRNA or shRNA targeting [NXT2](/details-gene/55916). Control and knockdown cells would then be stimulated with a TLR9 agonist (e.g., CpG-A). The functional consequence could be measured by quantifying IFN-α protein secretion via ELISA. To confirm the mechanism, subcellular fractionation followed by qRT-PCR could be used to measure the relative abundance of *IFNA* transcripts in the nuclear versus cytoplasmic fractions, with the expectation that [NXT2](/details-gene/55916) knockdown leads to nuclear retention of these transcripts. **Therapeutic Potential:** As a core component of the essential mRNA export machinery, [NXT2](/details-gene/55916) presents a challenging therapeutic target. Systemic **inhibition** would likely lead to significant toxicity across many healthy tissues. However, in diseases characterized by hyper-proliferation and transcriptional addiction, such as certain cancers, targeting nuclear export is a validated strategy. Therefore, inhibitors of [NXT2](/details-gene/55916) could have potential if they could be selectively delivered to malignant cells or if a therapeutic window exists where cancer cells are more sensitive to the inhibition of mRNA export than normal cells. Its role is fundamental, making activation an unlikely therapeutic strategy.

Genular Protein ID: 1486611698

Symbol: NXT2_HUMAN

Name: Protein p15-2

UniProtKB Accession Codes:

Q9NPJ8 D3DUY1 Q0VAN8 Q5JYV5 Q5JYV6 Q5JYV7 Q9H8U0 Q9NQ64 Q9NRL7 Q9Y3M4 Q9Y3M5

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 3 CDD 1 CTD 1 ChiTaRS 1 ComplexPortal 4 DNASU 1 EMBL 13 Ensembl 4 GO 9 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 RefSeq 4 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 11073998

Title: TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture.

PubMed ID: 11073998

DOI: 10.1128/mcb.20.23.8996-9008.2000

PubMed ID: 11042152

Title: Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells.

PubMed ID: 11042152

DOI: 10.1101/gr.140200

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15772651

Title: The DNA sequence of the human X chromosome.

PubMed ID: 15772651

DOI: 10.1038/nature03440

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 142
  • Mass: 16228
  • Checksum: 009B4E8929A6BA6C
  • Sequence:
    • MATSLDFKTY VDQACRAAEE FVNIYYETMD KRRRALTRLY LDKATLIWNG NAVSGLDALN 
NFFDTLPSSE FQVNMLDCQP VHEQATQSQT TVLVVTSGTV KFDGNKQHFF NQNFLLTAQS 
TPNNTVWKIA SDCFRFQDWS SS