Details for: TEKT1

Gene ID: 83659

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: TEKT1

Ensembl ID: ENSG00000167858

Description: tektin 1

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • glioblast CL0000030
    CSI 21.66
    rCSI 34.55%
    PRS 99.25
  • choroid plexus epithelial cell CL0000706
    CSI 19.57
    rCSI 32.05%
    PRS 99.19
  • ciliated epithelial cell CL0000067
    CSI 18.93
    rCSI 16.64%
    PRS 99.04
  • ependymal cell CL0000065
    CSI 15.5
    rCSI 31.46%
    PRS 97.64
  • multi-ciliated epithelial cell CL0005012
    CSI 14.75
    rCSI 14.72%
    PRS 99.32
  • ciliated cell CL0000064
    CSI 12.04
    rCSI 19.51%
    PRS 98.99
  • lung ciliated cell CL1000271
    CSI 10.64
    rCSI 12.3%
    PRS 99.38
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 7.15
    rCSI 16.3%
    PRS 99.08
  • deuterosomal cell CL4033044
    CSI 4.98
    rCSI 16.84%
    PRS 99
  • dopaminergic neuron CL0000700
    CSI 4.11
    rCSI 23.22%
    PRS 98.78
  • squamous epithelial cell CL0000076
    CSI 3.09
    rCSI 7.33%
    PRS 98.79

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [TEKT1](/details-gene/83659) (tektin 1) is a protein-coding gene located on chromosome 17p13.1. It encodes a structural component of microtubules, primarily within cilia and flagella. Functional annotations strongly associate [TEKT1](/details-gene/83659) with cilium assembly, motility, and the structure of the sperm flagellum. Expression data highlights its significant role in a variety of ciliated cell types, including those in the respiratory and central nervous systems. Notably, the gene shows the highest significance in [glioblast](/details-cell/CL0000030), suggesting a potential and less-understood role in the context of this brain malignancy. ## Cellular Roles and Expression Landscape The expression profile of [TEKT1](/details-gene/83659) underscores its fundamental role in the biology of ciliated cells. **Overall**, the gene demonstrates high significance in a consortium of cells defined by the presence of motile or primary cilia. - **Epithelial and CNS Ciliated Cells:** [TEKT1](/details-gene/83659) is a key marker for [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 18.93), [multi-ciliated epithelial cell](/details-cell/CL0005012) (CSI: 14.75), and specialized types such as [lung ciliated cell](/details-cell/CL1000271) (CSI: 10.64) and [ciliated columnar cell of tracheobronchial tree](/details-cell/CL0002145) (CSI: 7.15). Its importance extends to the central nervous system, with high significance in [choroid plexus epithelial cell](/details-cell/CL0000706) (CSI: 19.57) and [ependymal cell](/details-cell/CL0000065) (CSI: 15.50), both of which are ciliated cell types lining the brain's ventricles. - **Glioblastoma:** The most significant expression context for [TEKT1](/details-gene/83659) is in [glioblast](/details-cell/CL0000030) (CSI: 21.66), the malignant cell type of glioblastoma. This finding is unexpected for a gene primarily associated with normal ciliary function and may point towards a dysregulated role in cancer, potentially related to primary cilia signaling which is often altered in tumorigenesis. - **Neuronal Expression:** The gene also shows modest significance in [dopaminergic neuron](/details-cell/CL0000700) (CSI: 4.11), which is consistent with the recognized role of primary cilia in neuronal signaling and development. ## Pathways and Molecular Function Functional annotations reveal that [TEKT1](/details-gene/83659) is an integral structural protein of microtubule-based cellular appendages. - **Biological Process:** [TEKT1](/details-gene/83659) is involved in fundamental ciliary processes, including [cilium assembly](/details-cell/GO:0060271) and [cilium movement involved in cell motility](/details-cell/GO:0060294). Its specialized role in male fertility is highlighted by its participation in [flagellated sperm motility](/details-cell/GO:0030317). This is consistent with its discovery as a component of the sperm flagellum [Link](https://doi.org/10.1016/s1357-2725(01)00089-9). - **Molecular Function:** At the molecular level, its primary annotated function is [protein binding](/details-cell/GO:0005515), which is characteristic of a protein that forms filamentous polymers and interacts with other microtubule-associated proteins. - **Cellular Component:** [TEKT1](/details-gene/83659) is localized to the [microtubule cytoskeleton](/details-cell/GO:0015630), specifically within the [axonemal microtubule](/details-cell/GO:0005879) of the [sperm flagellum](/details-cell/GO:0036126). Recent proteomics research has further localized similar proteins to the [axonemal a tubule inner sheath](/details-cell/GO:0160111) [Link](https://doi.org/10.1073/pnas.2207605119). An additional localization to the [nucleus](/details-cell/GO:0005634) is noted, suggesting potential secondary functions outside of the cilium. ## Research Directions The established role of [TEKT1](/details-gene/83659) in ciliary biology combined with its top-ranking significance in glioblastoma opens several avenues for future investigation. **Proposed Hypotheses:** 1. Upregulation of [TEKT1](/details-gene/83659) in [glioblast](/details-cell/CL0000030) contributes to tumor progression by aberrantly modulating primary cilia-dependent signaling pathways, such as Sonic Hedgehog (SHH) or Wnt, which are known to be involved in cancer cell proliferation and survival. 2. [TEKT1](/details-gene/83659) expression is a marker of a specific glioblastoma subtype characterized by the presence of primary cilia, and its levels could correlate with therapeutic response to drugs targeting ciliary signaling pathways. **Experimental Approach:** To test the first hypothesis, a loss-of-function study could be performed. CRISPR-Cas9-mediated knockout of [TEKT1](/details-gene/83659) in patient-derived glioblastoma cell lines that show high endogenous expression would be a key first step. The impact on cell behavior could be assessed via in vitro assays measuring proliferation (e.g., cell counting, BrdU incorporation), migration (e.g., transwell assay), and invasiveness. Concurrently, the effect on signaling could be measured by quantifying the expression of downstream targets of the SHH pathway (e.g., GLI1, PTCH1) using qRT-PCR and Western blotting. **Therapeutic Potential:** As an intracellular structural protein, [TEKT1](/details-gene/83659) is not a conventional therapeutic target for small molecules or antibodies. However, if its expression proves to be essential for glioblastoma cell survival or proliferation, it could represent a novel target for therapeutic modalities designed to disrupt protein-protein interactions or for RNA-based therapies (e.g., siRNA, ASOs) aimed at inhibiting its expression. Given its high specificity for a malignant cell type in this context, such a strategy could potentially offer a therapeutic window with minimal side effects on non-ciliated tissues. Therefore, inhibition, rather than activation, would be the likely therapeutic goal.

Genular Protein ID: 513109328

Symbol: TEKT1_HUMAN

Name: Tektin-1

UniProtKB Accession Codes:

Q969V4 D3DTM7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 4 EMDB 2 Ensembl 1 ExpressionAtlas 1 GO 8 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 2 PDBsum 2 PRINTS 1 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 RefSeq 1 SIGNOR 1 SMR 1 STRING 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 11606253

Title: Cloning and characterization of a novel human TEKTIN1 gene.

PubMed ID: 11606253

DOI: 10.1016/s1357-2725(01)00089-9

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 36191189

Title: SPACA9 is a lumenal protein of human ciliary singlet and doublet microtubules.

PubMed ID: 36191189

DOI: 10.1073/pnas.2207605119

Sequence Information:

  • Length: 418
  • Mass: 48283
  • Checksum: 724A9172C627BA52
  • Sequence:
    • MAKLLQPPPK FLPSEWHIAN KNQYHRADAQ RSRSERLVAE SQRLVDEIEK TTRKSQSDVN 
KKLEQRLEEV QFWKKELDDK LEQLVNVTDD LLIYKIRLEK ALETLKEPLH ITETCLAYRE 
KRIGIDLVHD TVEHELIKEA EIIQGIMALL TRTLEEASEQ IRMNRSAKYN LEKDLKDKFV 
ALTIDDICFS LNNNSPNIRY SENAVRIEPN SVSLEDWLDF SSTNVEKADK QRNNSLMLKA 
LVDRILSQTA NDLRKQCDVV DTAFKNGLKD TKDARDKLAD HLAKVMEEIA SQEKNITALE 
KAILDQEGPA KVAHTRLETR THRPNVELCR DVAQYRLMKE VQEITHNVAR LKETLAQAQA 
ELKGLHRRQL ALQEEIQVKE NTIYIDEVLC MQMRKSIPLR DGEDHGVWAG GLRPDAVC