Details for: ZNF286A

Gene ID: 57335

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF286A

Ensembl ID: ENSG00000187607

Description: zinc finger protein 286A

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • progenitor cell CL0011026
    CSI 4.88
    rCSI 10.39%
    PRS 97.41
  • stem cell CL0000034
    CSI 3.09
    rCSI 2.98%
    PRS 98.99
  • mesodermal cell CL0000222
    CSI 2.89
    rCSI 3.46%
    PRS 99.47
  • VIP GABAergic cortical interneuron CL4023016
    CSI 2.56
    rCSI 3.06%
    PRS 96.98
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.56
    rCSI 3.29%
    PRS 97.4
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 2.44
    rCSI 3.04%
    PRS 96.64
  • peripheral nervous system neuron CL2000032
    CSI 2.42
    rCSI 3.3%
    PRS 98.12
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.16
    rCSI 3.82%
    PRS 97.08
  • cardiac muscle cell CL0000746
    CSI 2.15
    rCSI 3.08%
    PRS 97.31
  • retinal ganglion cell CL0000740
    CSI 1.42
    rCSI 3.13%
    PRS 96.99
  • indirect pathway medium spiny neuron CL4023029
    CSI 0.47
    rCSI 11.41%
    PRS 95.31
  • direct pathway medium spiny neuron CL4023026
    CSI 0.42
    rCSI 9.97%
    PRS 95.46

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Comma-separated if multiple.

Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF286A](/details-gene/57335) is a protein-coding gene located on chromosome 17p12 that encodes Zinc Finger Protein 286A. Functional annotations strongly suggest that it operates as a DNA-binding transcription factor involved in the regulation of transcription by RNA polymerase II. **Overall** expression analysis reveals its highest significance in undifferentiated cell types, including [progenitor cell](/details-cell/CL0011026)s and [stem cell](/details-cell/CL0000034)s, as well as in a wide range of mature neuronal subtypes. This expression pattern points towards a potential role in cellular development, lineage maintenance, and nervous system function. Initial characterization of this gene was part of large-scale cDNA cloning projects aimed at identifying novel human genes ([Link](https://doi.org/10.1093/dnares/8.2.85), [Link](https://doi.org/10.1038/ng1285)). ## Cellular Roles and Expression Landscape The expression profile of [ZNF286A](/details-gene/57335) indicates a primary role in undifferentiated and neurologically-active cells. **Overall**, the gene shows the highest cell significance index (CSI) in [progenitor cell](/details-cell/CL0011026) (CSI: 4.88), [stem cell](/details-cell/CL0000034) (CSI: 3.09), and [mesodermal cell](/details-cell/CL0000222) (CSI: 2.89), suggesting its involvement in maintaining pluripotency or directing early cell fate decisions. Concurrently, [ZNF286A](/details-gene/57335) is significantly expressed across a diverse set of neurons, highlighting a prominent role in the nervous system. This includes high significance in various GABAergic cortical interneurons such as [VIP GABAergic cortical interneuron](/details-cell/CL4023016) (CSI: 2.56) and [sst GABAergic cortical interneuron](/details-cell/CL4023017) (CSI: 2.56), as well as [peripheral nervous system neuron](/details-cell/CL2000032) (CSI: 2.42) and [retinal ganglion cell](/details-cell/CL0000740) (CSI: 1.42). This broad neuronal expression pattern suggests it may regulate fundamental aspects of neuronal identity or function rather than specifying a particular neuronal subtype. The gene also shows notable significance in [cardiac muscle cell](/details-cell/CL0000746) (CSI: 2.15), implying a potential function in cardiac tissue. ## Pathways and Molecular Function Functional annotations categorize [ZNF286A](/details-gene/57335) as a key component of the transcriptional machinery. Gene Ontology (GO) terms confirm its localization to the [nucleus](/details-go/GO:0005634) and its molecular function as a [dna-binding transcription factor activity, rna polymerase ii-specific](/details-go/GO:0000981) that binds to cis-regulatory regions ([GO:0000978](https://www.ebi.ac.uk/QuickGO/term/GO:0000978)). Its involvement in the biological process of [regulation of transcription by rna polymerase ii](/details-go/GO:0006357) is further supported by Reactome pathway data, which places it within the general frameworks of [gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and [rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857). This fundamental role in regulating gene expression is consistent with its high significance in developmentally plastic cells like [stem cell](/details-cell/CL0000034)s and progenitor cells, where precise transcriptional control is paramount for differentiation and self-renewal. ## Research Directions The dual expression pattern of [ZNF286A](/details-gene/57335) in both undifferentiated and terminally differentiated neuronal cells presents intriguing questions about its specific functions in these distinct cellular contexts. **Proposed Hypotheses:** 1. Given its high significance in [progenitor cell](/details-cell/CL0011026)s and [stem cell](/details-cell/CL0000034)s, [ZNF286A](/details-gene/57335) may function as a "gatekeeper" transcription factor that maintains cellular pluripotency or an undifferentiated state. Its downregulation may be a prerequisite for terminal differentiation into various lineages. 2. The broad expression across multiple, functionally distinct neuronal types suggests that [ZNF286A](/details-gene/57335) regulates a core set of genes essential for general neuronal homeostasis, survival, or synaptic function, rather than driving the specification of a single neuronal identity. **Experimental Approach:** To test the first hypothesis regarding its role in maintaining an undifferentiated state, a loss-of-function study in human induced pluripotent stem cells (iPSCs) could be performed. Using CRISPR-Cas9 to knock out [ZNF286A](/details-gene/57335), researchers could assess the impact on self-renewal capacity through colony-forming assays and expression of pluripotency markers like OCT4 and SOX2. Subsequently, both wild-type and knockout iPSCs could be subjected to directed differentiation protocols (e.g., towards neuronal or cardiac lineages). Changes in the efficiency and timing of differentiation could be monitored by RNA-sequencing and immunocytochemistry for lineage-specific markers, which would clarify whether [ZNF286A](/details-gene/57335) acts as a barrier to cellular differentiation. **Therapeutic Potential:** As an intracellular DNA-binding transcription factor, [ZNF286A](/details-gene/57335) presents a challenging direct target for small molecule therapeutics. However, if future research demonstrates its role as a master regulator of oncogenic stem-like states in certain cancers, it could become an important prognostic marker or an indirect therapeutic target. In such a scenario, strategies would likely focus on the inhibition of its activity or the disruption of its interaction with key co-factors. Further investigation into its specific downstream gene targets is necessary before its therapeutic relevance can be fully evaluated.

Genular Protein ID: 1036137594

Symbol: Z286A_HUMAN

Name: Zinc finger protein 286A

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 11347906

Title: Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro.

PubMed ID: 11347906

DOI: 10.1093/dnares/8.2.85

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16625196

Title: DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage.

PubMed ID: 16625196

DOI: 10.1038/nature04689

PubMed ID: 12168954

Title: Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones.

PubMed ID: 12168954

DOI: 10.1093/dnares/9.3.99

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

Sequence Information:

  • Length: 521
  • Mass: 60175
  • Checksum: 485C3FF3DD210DBB
  • Sequence:
  • METDLAEMPE KGALSSQDSP HFQEKSTEEG EVAALRLTAR SQETVTFKDV AMDFTPEEWG 
    KLDPAQRDVM LENYRNLVSL WLPVSKPESY NLENGKEPLK LERKAPKSSY SDMETRPQSK 
    DSTSVQDFSK AESCKVAIID RLTRNSVYDS NLEAALECEN WLENQQGNQE RHLREMFTHM 
    NSLSEETDHK HDVYWKSFNQ KSVLITEDRV PKGSYAFHTL EKSLKQKSNL MKKQRTYKEK 
    KPHKCNDCGE LFTYHSVLIR HQRVHTGEKP YTCNECGKSF SHRANLTKHQ RTHTRILFEC 
    SECKKTFTES SSLATHQRIH VGERPYECNE CGKGFNRSTH LVQHQLIHTG VKPYECNECD 
    KAFIHSSALI KHQRTHTGEK PYKCQECGKA FSHCSSLTKH QRVHTGEKPY ECSECGKTFS 
    QSTHLVQHQR IHTGEKPYEC NECGKTFSRS SNFAKHQRIH IGKKPYKCSE CGKAFIHSSA 
    LIQHQRTHTG EKPFRCNECG KSFKCSSSLI RHQRVHTEEQ P

Genular Protein ID: 2869199947

Symbol: A1A525_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

Sequence Information:

  • Length: 409
  • Mass: 47472
  • Checksum: 10FB740AF5529E2B
  • Sequence:
  • METRPQSKDS TSVQDFSKAE SCKVAIIDRL TRNSVYDSNL EAALECENWL ENQQGNQERH 
    LREMFTHMNS LSEETDHKHD VYWKSFNQKS VLITEDRVPK GSYAFHTLEK SLKQKSNLMK 
    KQRTYKEKKP HKCNDCGELF TYHSVLIRHQ RVHTGEKPYT CNECGKSFSH RANLTKHQRT 
    HTRILFECSE CKKTFTESSS LATHQRIHVG ERPYECNECG KGFNRSTHLV QHQLIHTGVK 
    PYECNECDKA FIHSSALIKH QRTHTGEKPY KCQECGKAFS HCSSLTKHQR VHTGEKPYEC 
    SECGKTFSQS THLVQHQRIH TGEKPYECNE CGKTFSRSSN FAKHQRIHIG KKPYKCSECG 
    KAFIHSSALI QHQRTHTGEK PFRCNECGKS FKCSSSLIRH QRVHTEEQP

Genular Protein ID: 545756873

Symbol: B2RCD9_HUMAN

Name: N/A

UniProtKB Accession Codes:

Database IDs:

Sequence Information:

  • Length: 521
  • Mass: 60147
  • Checksum: D5ABE554220BA2C1
  • Sequence:
  • METDLAEMPE KGALSSQDSP HFQEKSTEEG EVAALRLTAR SQETVTFKDV AMDFTPEEWG 
    KLDPAQRDVM LENYRNLVSL WLPVSKPESY NLENGKEPLK LERKAPKSSY SDMETRPQSK 
    DSTSVQDFSK AESCKVAIID RLTRNSVYDS NLEAALECEN WLENQQGNQE RHLREMFTHM 
    NSLSEETDHK HDVYWKSFNQ KSVLITEDRV PKGSYAFHTL EKSLKQKSNL MKKQRTYKEK 
    KPHKCNDCGE LFTYHSVLIR HQRVHTGEKP YTCNECGKSF SHRANLTKHQ RTHTKILFEC 
    SECKKTFTES SSLATHQRIH VGERPYECNE CGKGFNRSTH LVQHQLIHTG VKPYECNECD 
    KAFIHSSALI KHQRTHTGEK PYKCQECGKA FSHCSSLTKH QRVHTGEKPY ECSECGKTFS 
    QSTHLVQHQR IHTGEKPYEC NECGKTFSRS SNFAKHQRIH IGKKPYKCSE CGKAFIHSSA 
    LIQHQRTHTG EKPFRCNECG KSFKCSSSLI RHQRVHTEEQ P