Details for: ZNF574

Gene ID: 64763

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF574

Ensembl ID: ENSG00000105732

Description: zinc finger protein 574

Cell Significance Landscape

Associated with

  • Dna-binding transcription activator activity, rna polymerase ii-specific
    (GO:0001228)
  • Metal ion binding
    (GO:0046872)
  • Nucleus
    (GO:0005634)
  • Positive regulation of transcription by rna polymerase ii
    (GO:0045944)
  • Protein binding
    (GO:0005515)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • pro-B cell CL0000826
    CSI 4.24
    rCSI 3.51%
    PRS 98.48
  • common myeloid progenitor CL0000049
    CSI 3.45
    rCSI 2.79%
    PRS 98.95
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 3.21
    rCSI 2.9%
    PRS 97.93
  • group 3 innate lymphoid cell CL0001071
    CSI 2.96
    rCSI 2.22%
    PRS 98.72
  • keratinocyte CL0000312
    CSI 2.59
    rCSI 2.18%
    PRS 97.25
  • peripheral nervous system neuron CL2000032
    CSI 1.97
    rCSI 2.68%
    PRS 95.6
  • activated CD8-positive, alpha-beta T cell, human CL0001049
    CSI 1.94
    rCSI 3.31%
    PRS 99.1
  • basal cell CL0000646
    CSI 1.91
    rCSI 2.56%
    PRS 96.81
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.72
    rCSI 3.04%
    PRS 93.83

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF574](/details-gene/64763) is a protein-coding gene located on chromosome 19q13.2 that encodes Zinc Finger Protein 574, a member of the large family of zinc finger transcription factors ([Link](https://doi.org/10.1038/nature02399)). Functional annotations indicate that [ZNF574](/details-gene/64763) is localized to the [nucleus](/details-cell/GO:0005634) where it acts as a DNA-binding transcription activator, specifically involved in the [positive regulation of transcription by RNA polymerase II](/details-cell/GO:0045944). **Overall**, expression data reveals its highest significance in hematopoietic progenitor cells, including [pro-B cells](/details-cell/CL0000826), [common myeloid progenitors](/details-cell/CL0000049), and [megakaryocyte-erythroid progenitor cells](/details-cell/CL0000050), suggesting a primary role in regulating early blood cell development. ## Cellular Roles and Expression Landscape The expression profile of [ZNF574](/details-gene/64763) points to a significant role in the regulation of progenitor and developing cell populations across multiple lineages. * **Hematopoietic System:** The gene shows its most prominent significance in several hematopoietic progenitor populations. It is a top marker in [pro-B cells](/details-cell/CL0000826) (CSI: 4.24), [common myeloid progenitors](/details-cell/CL0000049) (CSI: 3.45), and [megakaryocyte-erythroid progenitor cells](/details-cell/CL0000050) (CSI: 3.21). This pattern strongly suggests that [ZNF574](/details-gene/64763) is a key transcriptional regulator involved in maintaining the state or guiding the differentiation of these early blood cell precursors. Its notable expression in [group 3 innate lymphoid cells](/details-cell/CL0001071) and [activated CD8-positive, alpha-beta T cells](/details-cell/CL0001049) further implies a potential role in the function of specific mature immune cell subsets. * **Epithelial and Neural Tissues:** Beyond the hematopoietic system, [ZNF574](/details-gene/64763) is also significantly expressed in epithelial cells, particularly [keratinocytes](/details-cell/CL0000312) and [basal cells](/details-cell/CL0000646). This indicates a possible function in skin development, homeostasis, or the maintenance of epidermal progenitor populations. Additionally, its expression in neuronal cell types, such as [peripheral nervous system neurons](/details-cell/CL2000032) and [caudal ganglionic eminence derived cortical interneurons](/details-cell/CL4023064), suggests a broader role in the development or function of the nervous system. ## Pathways and Molecular Function [ZNF574](/details-gene/64763) is annotated as a transcription factor with specific molecular functions consistent with this role. * **Transcriptional Regulation:** The primary molecular function of [ZNF574](/details-gene/64763) is [DNA-binding transcription activator activity, RNA polymerase II-specific](/details-cell/GO:0001228). It is involved in the biological process of [positive regulation of transcription by RNA polymerase II](/details-cell/GO:0045944), meaning it directly binds to DNA regulatory regions to initiate or enhance gene expression. This function is fundamental to controlling the complex gene expression programs required for cellular differentiation, a process highly active in the progenitor cells where [ZNF574](/details-gene/64763) is most significant. * **Molecular Interactions:** As a zinc finger protein, its function is dependent on [metal ion binding](/details-cell/GO:0046872), specifically zinc, which is crucial for maintaining the structural integrity of its DNA-binding domain. The protein is also annotated for [protein binding](/details-cell/GO:0005515), suggesting it likely operates as part of a larger multi-protein complex to regulate its target genes. ## Research Directions The expression pattern and annotated function of [ZNF574](/details-gene/64763) position it as a potentially important regulator of cell fate decisions, particularly in hematopoiesis. * **Testable Hypotheses:** 1. Given its high significance in multiple hematopoietic progenitor populations, it is hypothesized that [ZNF574](/details-gene/64763) is a master regulator that maintains the multipotent or progenitor state of early blood cells, and its downregulation is a prerequisite for terminal differentiation into mature lineages. 2. The notable expression in [keratinocytes](/details-cell/CL0000312) and [basal cells](/details-cell/CL0000646) suggests that [ZNF574](/details-gene/64763) may regulate the balance between proliferation and differentiation in epidermal stem cells, playing a role in skin homeostasis and wound repair. * **Proposed Experimental Approach:** To test the hypothesis regarding its role in hematopoiesis, one could utilize a loss-of-function approach. Specifically, CRISPR-Cas9-mediated knockout or shRNA-mediated knockdown of [ZNF574](/details-gene/64763) could be performed in primary human CD34+ hematopoietic stem and progenitor cells (HSPCs). These modified cells would then be cultured in vitro under conditions that promote differentiation towards myeloid, erythroid, and lymphoid lineages. The impact of [ZNF574](/details-gene/64763) loss could be assessed by colony-forming unit (CFU) assays and flow cytometry using lineage-specific markers. Furthermore, single-cell RNA sequencing (scRNA-seq) of the differentiating cultures would provide a high-resolution view of altered differentiation trajectories and allow for the identification of downstream target genes regulated by [ZNF574](/details-gene/64763). * **Therapeutic Potential:** As an intracellular transcription factor, [ZNF574](/details-gene/64763) presents a challenging drug target for traditional small-molecule inhibitors. However, if its dysregulation is implicated in diseases characterized by arrested differentiation, such as acute myeloid leukemia (AML), it could become a target for novel therapeutic modalities. For instance, technologies like proteolysis-targeting chimeras (PROTACs) could be developed to induce the targeted degradation of [ZNF574](/details-gene/64763), potentially forcing malignant progenitor cells to differentiate. A key consideration for such a strategy would be potential on-target toxicities in other tissues where the gene is expressed, such as the skin and nervous system, which may necessitate highly targeted delivery systems.

Genular Protein ID: 1061944294

Symbol: ZN574_HUMAN

Name: Zinc finger protein 574

UniProtKB Accession Codes:

Q6ZN55 Q6IPE0 Q6ZN10 Q7L5Z5 Q8NCE3 Q9H6N0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 6 Ensembl 2 ExpressionAtlas 1 GO 5 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 RefSeq 6 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24129315

Title: Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.

PubMed ID: 24129315

DOI: 10.1074/mcp.o113.027870

Sequence Information:

  • Length: 896
  • Mass: 98900
  • Checksum: F1E3EBCC47427654
  • Sequence:
    • MTEESEETVL YIEHRYVCSE CNQLYGSLEE VLMHQNSHVP QQHFELVGVA DPGVTVATDT 
ASGTGLYQTL VQESQYQCLE CGQLLMSPSQ LLEHQELHLK MMAPQEAVPA EPSPKAPPLS 
SSTIHYECVD CKALFASQEL WLNHRQTHLR ATPTKAPAPV VLGSPVVLGP PVGQARVAVE 
HSYRKAEEGG EGATVPSAAA TTTEVVTEVE LLLYKCSECS QLFQLPADFL EHQATHFPAP 
VPESQEPALQ QEVQASSPAE VPVSQPDPLP ASDHSYELRN GEAIGRDRRG RRARRNNSGE 
AGGAATQELF CSACDQLFLS PHQLQQHLRS HREGVFKCPL CSRVFPSPSS LDQHLGDHSS 
ESHFLCVDCG LAFGTEALLL AHRRAHTPNP LHSCPCGKTF VNLTKFLYHR RTHGVGGVPL 
PTTPVPPEEP VIGFPEPAPA ETGEPEAPEP PVSEETSAGP AAPGTYRCLL CSREFGKALQ 
LTRHQRFVHR LERRHKCSIC GKMFKKKSHV RNHLRTHTGE RPFPCPDCSK PFNSPANLAR 
HRLTHTGERP YRCGDCGKAF TQSSTLRQHR LVHAQHFPYR CQECGVRFHR PYRLLMHRYH 
HTGEYPYKCR ECPRSFLLRR LLEVHQLVVH AGRQPHRCPS CGAAFPSSLR LREHRCAAAA 
AQAPRRFECG TCGKKVGSAA RLQAHEAAHA AAGPGEVLAK EPPAPRAPRA TRAPVASPAA 
LGSTATASPA APARRRGLEC SECKKLFSTE TSLQVHRRIH TGERPYPCPD CGKAFRQSTH 
LKDHRRLHTG ERPFACEVCG KAFAISMRLA EHRRIHTGER PYSCPDCGKS YRSFSNLWKH 
RKTHQQQHQA AVRQQLAEAE AAVGLAVMET AVEALPLVEA IEIYPLAEAE GVQISG

Genular Protein ID: 2284634734

Symbol: A0A0C4DFM2_HUMAN

Name: N/A

UniProtKB Accession Codes:

A0A0C4DFM2

Database IDs:

ARBA 6 Antibodypedia 1 Bgee 1 BioGRID-ORCS 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 3 Ensembl 2 GO 2 Gene3D 1 GeneTree 1 HGNC 1 InterPro 2 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PROSITE 3 PROSITE-ProRule 1 PeptideAtlas 1 Pfam 3 Proteomes 2 ProteomicsDB 1 RefSeq 1 SAM 1 SMART 1 SUPFAM 1 VEuPathDB 1

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 11181995

Title: The sequence of the human genome.

PubMed ID: 11181995

DOI: 10.1126/science.1058040

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

PubMed ID: 16964243

Title: A probability-based approach for high-throughput protein phosphorylation analysis and site localization.

PubMed ID: 16964243

DOI: 10.1038/nbt1240

PubMed ID: 18220336

Title: Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.

PubMed ID: 18220336

DOI: 10.1021/pr0705441

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

PubMed ID: 24129315

Title: Immunoaffinity enrichment and mass spectrometry analysis of protein methylation.

PubMed ID: 24129315

Sequence Information:

  • Length: 986
  • Mass: 108704
  • Checksum: D511253327A89033
  • Sequence:
    • MPRATWANSK ERSWAESERG PRDTGNGGSK AERHIQEIET GRGGDRAKAH RRQRMRLRGT 
ERASLGPGRR LGDSRGTDMP GARAQGLAAA MTEESEETVL YIEHRYVCSE CNQLYGSLEE 
VLMHQNSHVP QQHFELVGVA DPGVTVATDT ASGTGLYQTL VQESQYQCLE CGQLLMSPSQ 
LLEHQELHLK MMAPQEAVPA EPSPKAPPLS SSTIHYECVD CKALFASQEL WLNHRQTHLR 
ATPTKAPAPV VLGSPVVLGP PVGQARVAVE HSYRKAEEGG EGATVPSAAA TTTEVVTEVE 
LLLYKCSECS QLFQLPADFL EHQATHFPAP VPESQEPALQ QEVQASSPAE VPVSQPDPLP 
ASDHSYELRN GEAIGRDRRG RRARRNNSGE AGGAATQELF CSACDQLFLS PHQLQQHLRS 
HREGVFKCPL CSRVFPSPSS LDQHLGDHSS ESHFLCVDCG LAFGTEALLL AHRRAHTPNP 
LHSCPCGKTF VNLTKFLYHR RTHGVGGVPL PTTPVPPEEP VIGFPEPAPA ETGEPEAPEP 
PVSEETSAGP AAPGTYRCLL CSREFGKALQ LTRHQRFVHR LERRHKCSIC GKMFKKKSHV 
RNHLRTHTGE RPFPCPDCSK PFNSPANLAR HRLTHTGERP YRCGDCGKAF TQSSTLRQHR 
LVHAQHFPYR CQECGVRFHR PYRLLMHRYH HTGEYPYKCR ECPRSFLLRR LLEVHQLVVH 
AGRQPHRCPS CGAAFPSSLR LREHRCAAAA AQAPRRFECG TCGKKVGSAA RLQAHEAAHA 
AAGPGEVLAK EPPAPRAPRA TRAPVASPAA LGSTATASPA APARRRGLEC SECKKLFSTE 
TSLQVHRRIH TGERPYPCPD CGKAFRQSTH LKDHRRLHTG ERPFACEVCG KAFAISMRLA 
EHRRIHTGER PYSCPDCGKS YRSFSNLWKH RKTHQQQHQA AVRQQLAEAE AAVGLAVMET 
AVEALPLVEA IEIYPLAEAE GVQISG

Genular Protein ID: 1432698603

Symbol: Q71MF7_HUMAN

Name: N/A

UniProtKB Accession Codes:

Q71MF7

Database IDs:

ARBA 4 AlphaFoldDB 1 BioGRID-ORCS 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 2 GO 1 Gene3D 1 InterPro 2 KEGG 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PROSITE 3 PROSITE-ProRule 1 PeptideAtlas 1 Pfam 2 RefSeq 2 SAM 1 SMART 1 SUPFAM 1

Sequence Information:

  • Length: 545
  • Mass: 60747
  • Checksum: 47B52A125FADCEEF
  • Sequence:
    • MSMWEDLCQP YQVPLSPAYS WGRGGVPLPT TPVPPEEPVI GFPEPAPAET GEPEAPEPPV 
SEETSAGPAA PGTYRCLLCS REFGKALQLT RHQRFVHRLE RRHKCSICGK MFKKKSHVRN 
HLRTHTGERP FPCPDCSKPF NSPANLARHR LTHTGERPYR CGDCGKAFTQ SSTLRQHRLV 
HAQHFPYRCQ ECGVRFHRPY RLLMHRYHHT GEYPYKCREC PRSFLLRRLL EVHQLVVHAG 
RQPHRCPSCG AAFPSSLRLR EHRCAAAAAQ APRRFECGTC GKKVGSAARL QAHEAAHAAA 
GPGEVLAKEP PAPRAPRATR APVASPAALG STATASPAAP ARRRGLECSE CKKLFSTETS 
LQVHRRIHTG ERPYPCPDCG KAFRQSTHLK DTGACTQVSG PLPVKCVARP LPSPCAWQNI 
AASTQANDPT PALTVARATA PSPTSGSTAR PISSSIRQLC GSSWQRRRLP LAWPSWRLLW 
RRYPWWKPLR STLWPRLRGS RSVADSARLP LWHLHSLLLK ALQHPLKHLY ILCPFLFPSP 
PPCKF