Details for: DEPDC7

Gene ID: 91614

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: DEPDC7

Ensembl ID: ENSG00000121690

Description: DEP domain containing 7

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • midzonal region hepatocyte CL0019028
    CSI 4.11
    rCSI 9.64%
    PRS 91.75
  • intestinal tuft cell CL0019032
    CSI 3.37
    rCSI 5.15%
    PRS 95.36
  • intestinal epithelial cell CL0002563
    CSI 3.03
    rCSI 3.16%
    PRS 92.84
  • hepatocyte CL0000182
    CSI 2.33
    rCSI 4.18%
    PRS 93.16
  • periportal region hepatocyte CL0019026
    CSI 2.04
    rCSI 7.94%
    PRS 91.43
  • centrilobular region hepatocyte CL0019029
    CSI 1.96
    rCSI 5.12%
    PRS 90.93
  • extravillous trophoblast CL0008036
    CSI 1.84
    rCSI 2.27%
    PRS 93.21
  • tuft cell of colon CL0009041
    CSI 1.78
    rCSI 4.16%
    PRS 95.5
  • colonocyte CL1000347
    CSI 1.73
    rCSI 2.47%
    PRS 92.99
  • melanocyte of skin CL1000458
    CSI 0.96
    rCSI 1.31%
    PRS 70.03

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [DEPDC7](/details-gene/91614) (DEP domain containing 7) is a protein-coding gene located on chromosome 11p13 in humans. As its name suggests, the protein product contains a DEP (Dishevelled, Egl-10, and Pleckstrin) domain, which is typically involved in mediating protein-protein interactions within intracellular signaling pathways. **Overall**, expression data reveals a highly specific enrichment of [DEPDC7](/details-gene/91614) in epithelial cells of the liver and gastrointestinal tract, particularly in [midzonal region hepatocyte](/details-cell/CL0019028) and [intestinal tuft cell](/details-cell/CL0019032). This expression pattern suggests a specialized role in the metabolic, absorptive, or signaling functions of these vital organs. The gene was initially identified and mapped as part of large-scale human genome sequencing and annotation efforts ([Link](https://doi.org/10.1101/gr.9.11.1074), [Link](https://doi.org/10.1038/nature04632)). ## Cellular Roles and Expression Landscape The expression profile of [DEPDC7](/details-gene/91614) points to a primary function within specialized epithelial tissues. The gene exhibits its highest significance in liver cells, with notable expression across various functional zones, including [midzonal region hepatocyte](/details-cell/CL0019028) (CSI: 4.11), [periportal region hepatocyte](/details-cell/CL0019026) (CSI: 2.04), and [centrilobular region hepatocyte](/details-cell/CL0019029) (CSI: 1.96). This broad expression across hepatocyte subtypes suggests its involvement in a core homeostatic function of the liver rather than a role specific to a particular metabolic zone. In parallel, [DEPDC7](/details-gene/91614) shows strong significance in the intestinal epithelium. It is a key marker for [intestinal tuft cell](/details-cell/CL0019032) (CSI: 3.37) and its colonic counterpart, [tuft cell of colon](/details-cell/CL0009041) (CSI: 1.78), which are chemosensory cells that regulate mucosal immunity and tissue repair. Furthermore, its expression in [intestinal epithelial cell](/details-cell/CL0002563) (CSI: 3.03) and [colonocyte](/details-cell/CL1000347) (CSI: 1.73) highlights a broader role in intestinal biology, potentially related to barrier integrity or nutrient processing. The gene's presence has been confirmed through full-length cDNA projects like the Mammalian Gene Collection ([Link](https://doi.org/10.1101/gr.2596504)). ## Pathways and Molecular Function The functional annotation for [DEPDC7](/details-gene/91614) is currently broad, reflecting a need for further characterization. Gene Ontology terms classify it under general categories such as [Biological_process](/details-go/GO:0008150) ([GO:0008150](https://www.ebi.ac.uk/QuickGO/term/GO:0008150)) and [Molecular_function](/details-go/GO:0003674) ([GO:0003674](https://www.ebi.ac.uk/QuickGO/term/GO:0003674)). More specifically, it is associated with [Intracellular signal transduction](/details-go/GO:0035556) ([GO:0035556](https://www.ebi.ac.uk/QuickGO/term/GO:0035556)), which is consistent with the presence of a DEP domain. This domain is known to act as a scaffold or localization signal, directing proteins to specific membrane compartments to participate in signaling cascades. The precise pathways in which [DEPDC7](/details-gene/91614) participates in hepatocytes and intestinal cells remain to be elucidated. ## Research Directions The specific and high-level expression of [DEPDC7](/details-gene/91614) in hepatocytes and intestinal epithelial cells provides a clear path for future investigation into its physiological roles and potential involvement in disease. **Proposed Hypotheses:** 1. **Hypothesis 1:** Given its robust expression across all hepatocyte zones, [DEPDC7](/details-gene/91614) may be a critical component of a core liver metabolic pathway, such as lipid metabolism, glucose homeostasis, or biotransformation of xenobiotics. Its disruption could contribute to metabolic disorders like non-alcoholic fatty liver disease (NAFLD). 2. **Hypothesis 2:** The high significance of [DEPDC7](/details-gene/91614) in [intestinal tuft cell](/details-cell/CL0019032) suggests it may function downstream of the chemosensory receptors in these cells, transducing signals that initiate type 2 immune responses or regulate epithelial cell turnover in response to luminal contents (e.g., parasites or allergens). **Experimental Approach:** To test the role of [DEPDC7](/details-gene/91614) in liver metabolism (Hypothesis 1), a targeted knockdown using siRNA or a knockout using CRISPR-Cas9 could be performed in a human hepatocyte cell line (e.g., HepG2 or Huh7). The functional consequences could be assessed by exposing the modified cells to metabolic challenges, such as high glucose or free fatty acids, followed by analysis of key metabolic outputs. For instance, RNA-sequencing could reveal dysregulated metabolic pathways, while assays for lipid accumulation (Oil Red O staining) and glucose production could directly measure functional deficits. **Therapeutic Potential:** Currently, the therapeutic potential of [DEPDC7](/details-gene/91614) is speculative due to its uncharacterized function. As an intracellular signaling protein, it would likely be targeted by small molecule inhibitors rather than biologics. If future studies link its dysregulation to diseases like liver cancer (hepatocellular carcinoma) or inflammatory bowel disease—conditions affecting tissues where it is highly expressed—it could emerge as a potential therapeutic target. However, its high expression in healthy, vital tissues like the liver and intestine suggests that systemic inhibition could lead to significant on-target toxicity, necessitating the development of highly specific, tissue-targeted therapeutic strategies.

Genular Protein ID: 3937812362

Symbol: DEPD7_HUMAN

Name: DEP domain-containing protein 7

UniProtKB Accession Codes:

Q96QD5 G5E941 Q8N602 Q8NCU9 Q9UGK5

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CDD 2 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 7 Ensembl 2 GO 1 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 3 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 RefSeq 2 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 10568747

Title: A 7.5 Mb sequence-ready PAC contig and gene expression map of human chromosome 11p13-p14.1.

PubMed ID: 10568747

DOI: 10.1101/gr.9.11.1074

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 511
  • Mass: 58310
  • Checksum: F69089ABE41AD2EA
  • Sequence:
    • MATVQEKAAA LNLSALHSPA HRPPGFSVAQ KPFGATYVWS SIINTLQTQV EVKKRRHRLK 
RHNDCFVGSE AVDVIFSHLI QNKYFGDVDI PRAKVVRVCQ ALMDYKVFEA VPTKVFGKDK 
KPTFEDSSCS LYRFTTIPNQ DSQLGKENKL YSPARYADAL FKSSDIRSAS LEDLWENLSL 
KPANSPHVNI SATLSPQVIN EVWQEETIGR LLQLVDLPLL DSLLKQQEAV PKIPQPKRQS 
TMVNSSNYLD RGILKAYSDS QEDEWLSAAI DCLEYLPDQM VVEISRSFPE QPDRTDLVKE 
LLFDAIGRYY SSREPLLNHL SDVHNGIAEL LVNGKTEIAL EATQLLLKLL DFQNREEFRR 
LLYFMAVAAN PSEFKLQKES DNRMVVKRIF SKAIVDNKNL SKGKTDLLVL FLMDHQKDVF 
KIPGTLHKIV SVKLMAIQNG RDPNRDAGYI YCQRIDQRDY SNNTEKTTKD ELLNLLKTLD 
EDSKLSAKEK KKLLGQFYKC HPDIFIEHFG D