Details for: ZNF600

Gene ID: 162966

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF600

Ensembl ID: ENSG00000189190

Description: zinc finger protein 600

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

  • Gene expression (transcription)
    (R-HSA-74160)
  • Generic transcription pathway
    (R-HSA-212436)
  • Rna polymerase ii transcription
    (R-HSA-73857)
  • Dna-binding transcription activator activity, rna polymerase ii-specific
    (GO:0001228)
  • Metal ion binding
    (GO:0046872)
  • Nucleus
    (GO:0005634)
  • Positive regulation of transcription by rna polymerase ii
    (GO:0045944)
  • Protein binding
    (GO:0005515)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 4.75
    rCSI 3.61%
    PRS 99.56
  • CD4-positive, alpha-beta memory T cell CL0000897
    CSI 4.74
    rCSI 3.4%
    PRS 99.62
  • class switched memory B cell CL0000972
    CSI 3.7
    rCSI 2.76%
    PRS 98.96
  • CD8-positive, alpha-beta cytotoxic T cell CL0000794
    CSI 3.69
    rCSI 4.4%
    PRS 99.28
  • central memory CD8-positive, alpha-beta T cell CL0000907
    CSI 3.28
    rCSI 2.21%
    PRS 99.68
  • keratinocyte CL0000312
    CSI 3.09
    rCSI 2.59%
    PRS 97.21
  • activated type II NK T cell CL0000931
    CSI 2.75
    rCSI 3.09%
    PRS 99.22
  • conjunctival epithelial cell CL1000432
    CSI 2.74
    rCSI 4.19%
    PRS 97.29
  • BEST4+ enteroycte CL4030026
    CSI 2.52
    rCSI 3.13%
    PRS 97.35
  • extravillous trophoblast CL0008036
    CSI 2.23
    rCSI 2.76%
    PRS 97.31
  • effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062
    CSI 0.54
    rCSI 2.72%
    PRS 99.42

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF600](/details-gene/162966) is a protein-coding gene located on chromosome 19 that encodes Zinc Finger Protein 600. Based on its functional annotations, [ZNF600](/details-gene/162966) is characterized as a nuclear DNA-binding transcription activator ([GO:0001228](https://www.ebi.ac.uk/QuickGO/term/GO:0001228), [GO:0005634](https://www.ebi.ac.uk/QuickGO/term/GO:0005634)). It is predicted to play a key role in the positive regulation of transcription by RNA polymerase II ([GO:0045944](https://www.ebi.ac.uk/QuickGO/term/GO:0045944)). **Overall**, expression data reveals its high significance in multiple subsets of adaptive immune cells, particularly effector and memory [T cells](/details-cell/CL0000084) and memory [B cells](/details-cell/CL0000236), suggesting a crucial role in regulating transcriptional programs associated with lymphocyte activation, differentiation, and memory. ## Cellular Roles and Expression Landscape The expression profile of [ZNF600](/details-gene/162966) highlights its prominence within the immune system. **Overall**, the gene shows the highest significance in various lymphocyte populations involved in adaptive immunity. It is a top marker for [effector CD8-positive, alpha-beta T cells](/details-cell/CL0001050) (CSI: 4.75), [CD4-positive, alpha-beta memory T cells](/details-cell/CL0000897) (CSI: 4.74), and [class switched memory B cells](/details-cell/CL0000972) (CSI: 3.70). Its high significance extends to other cytotoxic and memory lymphocytes, including [CD8-positive, alpha-beta cytotoxic T cells](/details-cell/CL0000794) and [central memory CD8-positive, alpha-beta T cells](/details-cell/CL0000907). This consistent pattern across multiple activated and memory lymphocyte lineages suggests that [ZNF600](/details-gene/162966) may be a key transcriptional regulator orchestrating late-stage differentiation and long-term persistence of these cells. Beyond the adaptive immune system, [ZNF600](/details-gene/162966) also shows significant expression in several epithelial cell types, such as [keratinocytes](/details-cell/CL0000312) (CSI: 3.09) and [conjunctival epithelial cells](/details-cell/CL1000432) (CSI: 2.74). This suggests a potential role in epithelial biology, possibly related to differentiation or barrier function, which is distinct from its role in immunity. ## Pathways and Molecular Function [ZNF600](/details-gene/162966) functions as a transcription factor, a role substantiated by its molecular function annotations. It is classified with *DNA-binding transcription activator activity, RNA polymerase II-specific* ([GO:0001228](https://www.ebi.ac.uk/QuickGO/term/GO:0001228)) and the ability to bind metal ions ([GO:0046872](https://www.ebi.ac.uk/QuickGO/term/GO:0046872)), which is characteristic of zinc finger proteins. Its primary biological process is the *positive regulation of transcription by RNA polymerase II* ([GO:0045944](https://www.ebi.ac.uk/QuickGO/term/GO:0045944)), placing it as an activator of gene expression. This function is consistent with its involvement in broad transcriptional pathways detailed by Reactome, including *Gene expression (transcription)* ([R-HSA-74160](https://reactome.org/content/detail/R-HSA-74160)) and *RNA polymerase II transcription* ([R-HSA-73857](https://reactome.org/content/detail/R-HSA-73857)). Given its high expression in effector lymphocytes, it is plausible that [ZNF600](/details-gene/162966) activates genes critical for T cell effector functions, a hypothesis supported by studies identifying phosphoproteins involved in T cell receptor signaling ([Link](https://doi.org/10.1126/scisignal.2000007)). Its localization to the [nucleus](/details-cell/GO:0005634) ([GO:0005634](https://www.ebi.ac.uk/QuickGO/term/GO:0005634)) is in line with its role as a regulator of gene transcription. ## Research Directions Based on its expression profile and annotated functions, [ZNF600](/details-gene/162966) presents several avenues for future research, particularly in immunology and epithelial biology. **Testable Hypotheses:** 1. **[ZNF600](/details-gene/162966) is a master regulator of T cell effector differentiation:** The gene's high significance in [effector CD8-positive, alpha-beta T cells](/details-cell/CL0001050) and [CD8-positive, alpha-beta cytotoxic T cells](/details-cell/CL0000794) suggests it may directly regulate the expression of key cytotoxic molecules (e.g., granzymes, perforin) and cytokines (e.g., IFN-gamma) during T cell activation. 2. **[ZNF600](/details-gene/162966) is essential for the maintenance of immunological memory:** Its high CSI in multiple memory lymphocyte subsets ([CD4-positive, alpha-beta memory T cell](/details-cell/CL0000897), [class switched memory B cell](/details-cell/CL0000972), [central memory CD8-positive, alpha-beta T cell](/details-cell/CL0000907)) points to a potential role in controlling the transcriptional programs that sustain long-lived memory cells. **Proposed Experiment:** To test the first hypothesis, a loss-of-function study could be performed. Primary human CD8+ T cells could be isolated and subjected to CRISPR-Cas9-mediated knockout of [ZNF600](/details-gene/162966). Following T-cell receptor stimulation *in vitro*, the functional consequences would be assessed. This would involve quantifying the expression of key effector genes (*GZMB*, *PRF1*, *IFNG*) via RNA-seq and measuring the cells' cytotoxic capacity against target cells in a co-culture killing assay. A significant reduction in these functions in knockout cells compared to controls would validate the hypothesis. **Therapeutic Potential:** As an intracellular transcription factor, [ZNF600](/details-gene/162966) is not a conventional drug target for antibodies. However, if it is proven to be a critical driver of pathogenic T cell responses in autoimmune diseases or a factor in T cell exhaustion in cancer, it could become a target for novel therapeutic modalities. Strategies aimed at **inhibition**, such as antisense oligonucleotides or targeted protein degraders (e.g., PROTACs), could be explored to modulate its activity and dampen aberrant immune responses. Its high specificity for certain lymphocyte subsets may offer a therapeutic window with limited off-target effects on other tissues.

Genular Protein ID: 1571835940

Symbol: ZN600_HUMAN

Name: Zinc finger protein 600

UniProtKB Accession Codes:

Q6ZNG1 Q6MZR0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 3 Ensembl 1 ExpressionAtlas 1 GO 5 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MassIVE 1 NCBI Taxonomy 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 Reactome 1 RefSeq 3 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

Sequence Information:

  • Length: 722
  • Mass: 83124
  • Checksum: E316D2E12AFB59F9
  • Sequence:
    • MMKEVLSTGQ GNTEVIHTGT LQRYQSYHIG DFCFQEIEKE IHDIEFQCQE DERNGHEAPM 
TKIKKLTGST DQHDHRHAGN KPIKDQLGSS FYSHLPELHI IQIKGKIGNQ FEKSTSDAPS 
VSTSQRISPR PQIHISNNYG NNSPNSSLLP QKQEVYMREK SFQCNESGKA FNCSSLLRKH 
QIPHLGDKQY KCDVCGKLFN HKQYLTCHCR CHTGEKPYKC NECGKSFSQV SSLTCHRRLH 
TAVKSHKCNE CGKIFGQNSA LVIHKAIHTG EKPYKCNECD KAFNQQSNLA RHRRIHTGEK 
PYKCEECDKV FSRKSTLESH KRIHTGEKPY KCKVCDTAFT WNSQLARHKR IHTGEKTYKC 
NECGKTFSHK SSLVCHHRLH GGEKSYKCKV CDKAFAWNSH LVRHTRIHSG GKPYKCNECG 
KTFGQNSDLL IHKSIHTGEQ PYKYEECEKV FSCGSTLETH KIIHTGEKPY KCKVCDKAFA 
CHSYLAKHTR IHSGEKPYKC NECSKTFRLR SYLASHRRVH SGEKPYKCNE CSKTFSQRSY 
LHCHRRLHSG EKPYKCNECG KTFSHKPSLV HHRRLHTGEK SYKCTVCDKA FVRNSYLARH 
TRIHTAEKPY KCNECGKAFN QQSQLSLHHR IHAGEKLYKC ETCDKVFSRK SHLKRHRRIH 
PGKKPYKCKV CDKTFGSDSH LKQHTGLHTG EKPYKCNECG KAFSKQSTLI HHQAVHGVGK 
LD

Genular Protein ID: 1135769566

Symbol: A0A3B3IT03_HUMAN

Name: N/A

UniProtKB Accession Codes:

A0A3B3IT03

Database IDs:

ARBA 4 Antibodypedia 1 Bgee 1 CDD 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 3 Ensembl 5 GO 2 Gene3D 2 GeneTree 1 HGNC 1 InterPro 4 MANE-Select 1 NCBI Taxonomy 1 OMA 1 OrthoDB 1 PANTHER 2 PROSITE 5 PROSITE-ProRule 1 PeptideAtlas 1 Pfam 3 Proteomes 2 ProteomicsDB 1 RefSeq 5 SAM 1 SMART 2 SUPFAM 2 VEuPathDB 1

Citations:

PubMed ID: 11237011

Title: Initial sequencing and analysis of the human genome.

PubMed ID: 11237011

DOI: 10.1038/35057062

PubMed ID: 11181995

Title: The sequence of the human genome.

PubMed ID: 11181995

DOI: 10.1126/science.1058040

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15496913

Title: Finishing the euchromatic sequence of the human genome.

PubMed ID: 15496913

DOI: 10.1038/nature03001

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

Sequence Information:

  • Length: 791
  • Mass: 91093
  • Checksum: DC7B575753CA0306
  • Sequence:
    • MLCEEAAQKR KGKEPGMALP QGRLTFRDVA IEFSLAEWKC LNPSQRALYR EVMLENYRNL 
EAVDISSKRM MKEVLSTGQG NTEVIHTGTL QRYQSYHIGD FCFQEIEKEI HDIEFQCQED 
ERNGHEAPMT KIKKLTGSTD QHDHRHAGNK PIKDQLGSSF YSHLPELHII QIKGKIGNQF 
EKSTSDAPSV STSQRISPRP QIHISNNYGN NSPNSSLLPQ KQEVYMREKS FQCNESGKAF 
NCSSLLRKHQ IPHLGDKQYK CDVCGKLFNH KQYLTCHCRC HTGEKPYKCN ECGKSFSQVS 
SLTCHRRLHT AVKSHKCNEC GKIFGQNSAL VIHKAIHTGE KPYKCNECDK AFNQQSNLAR 
HRRIHTGEKP YKCEECDKVF SRKSTLESHK RIHTGEKPYK CKVCDTAFTW NSQLARHKRI 
HTGEKTYKCN ECGKTFSHKS SLVCHHRLHG GEKSYKCKVC DKAFAWNSHL VRHTRIHSGG 
KPYKCNECGK TFGQNSDLLI HKSIHTGEQP YKYEECEKVF SCGSTLETHK IIHTGEKPYK 
CKVCDKAFAC HSYLAKHTRI HSGEKPYKCN ECSKTFRLRS YLASHRRVHS GEKPYKCNEC 
SKTFSQRSYL HCHRRLHSGE KPYKCNECGK TFSHKPSLVH HRRLHTGEKS YKCTVCDKAF 
VRNSYLARHT RIHTAEKPYK CNECGKAFNQ QSQLSLHHRI HAGEKLYKCE TCDKVFSRKS 
HLKRHRRIHP GKKPYKCKVC DKTFGSDSHL KQHTGLHTGE KPYKCNECGK AFSKQSTLIH 
HQAVHGVGKL D