Details for: TBC1D8 AS1

Gene ID: 100506286

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: TBC1D8 AS1

Ensembl ID: ENSG00000272902

Description: TBC1D8 antisense RNA 1

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203
    CSI 80.37
    rCSI 97.38%
    PRS 0.81
  • melanocyte of skin CL1000458
    CSI 74.91
    rCSI 100%
    PRS 0.4
  • conventional dendritic cell CL0000990
    CSI 72.52
    rCSI 60.54%
    PRS 1.69
  • suprabasal keratinocyte CL4033013
    CSI 66.78
    rCSI 100%
    PRS 0.5
  • mast cell CL0000097
    CSI 58.61
    rCSI 100%
    PRS 2.94
  • chondrocyte CL0000138
    CSI 38.75
    rCSI 61.63%
    PRS 0.47
  • basal cell of epidermis CL0002187
    CSI 38.6
    rCSI 68.42%
    PRS 0.66
  • regulatory T cell CL0000815
    CSI 33.79
    rCSI 39.17%
    PRS 1.84
  • cytotoxic T cell CL0000910
    CSI 7.63
    rCSI 43.7%
    PRS 0.83
  • helper T cell CL0000912
    CSI -9.08
    rCSI -12.83%
    PRS 0.8
  • pericyte CL0000669
    CSI -28.34
    rCSI -75.46%
    PRS 4.96
  • innate lymphoid cell CL0001065
    CSI -47.95
    rCSI -99%
    PRS 0.88

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [TBC1D8 AS1](/details-gene/100506286) is a non-coding antisense RNA located on chromosome 2. As an antisense transcript, its primary function is presumed to be the regulation of its sense-strand counterpart, TBC1D8, a protein involved in vesicular trafficking. Gene expression analysis indicates that [TBC1D8 AS1](/details-gene/100506286) is a significant transcript in distinct cell populations, showing high expression in both immune cells, such as [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), and various structural cells of the skin, including [melanocyte of skin](/details-cell/CL1000458) and [suprabasal keratinocyte](/details-cell/CL4033013). This dual expression pattern suggests a potential role in modulating cellular processes at the intersection of immunity and tissue homeostasis. ## Cellular Roles and Expression Landscape The expression profile of [TBC1D8 AS1](/details-gene/100506286) suggests specialized roles in the immune system and cutaneous tissues. **Overall**, its significance is highest in several key immune cell types, including [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), [conventional dendritic cell](/details-cell/CL0000990), [mast cell](/details-cell/CL0000097), and [regulatory T cell](/details-cell/CL0000815). This pattern points towards a function in adaptive and innate immunity. Interestingly, the gene's expression is highly specific within the T cell compartment; while prominent in cytotoxic and regulatory lineages, it is notably low in [helper T cell](/details-cell/CL0000912) and virtually absent in [innate lymphoid cell](/details-cell/CL0001065). This differential expression may indicate a role in governing the specific functions or differentiation pathways of T cell subsets. Concurrently, [TBC1D8 AS1](/details-gene/100506286) shows high significance in skin cells, including [melanocyte of skin](/details-cell/CL1000458), [suprabasal keratinocyte](/details-cell/CL4033013), and [basal cell of epidermis](/details-cell/CL0002187). This co-expression in both epithelial and immune cells of the skin suggests a potential contribution to cutaneous immune surveillance or tissue barrier function. The gene's specificity is further highlighted by its low expression in mesenchymal cell types like the [pericyte](/details-cell/CL0000669), underscoring its constrained role outside of these immunological and epithelial contexts. ## Pathways and Molecular Function Specific functional pathway annotations for [TBC1D8 AS1](/details-gene/100506286) are not provided in the dataset. However, as an antisense RNA, its molecular function is likely mediated through the regulation of the TBC1D8 sense transcript. The TBC1D8 protein is a Rab-GTPase activating protein (Rab-GAP) that plays a critical role in controlling intracellular membrane trafficking, including endocytosis and autophagy. Therefore, the cell-specific expression of [TBC1D8 AS1](/details-gene/100506286) likely fine-tunes these fundamental trafficking pathways. In [conventional dendritic cell](/details-cell/CL0000990), this regulation could modulate antigen processing and presentation. In memory and [regulatory T cell](/details-cell/CL0000815), it might influence receptor turnover, cellular metabolism, or long-term survival, processes heavily dependent on precise control of vesicular transport. ## Research Directions The distinct and specific expression pattern of [TBC1D8 AS1](/details-gene/100506286) prompts several avenues for future investigation. ### Proposed Hypotheses: 1. **[TBC1D8 AS1](/details-gene/100506286) is a critical regulator of T cell lineage fate.** Given its high expression in CD8+ and regulatory T cells but low expression in helper T cells, it may act as a molecular switch that influences differentiation by modulating TBC1D8-mediated trafficking of key lineage-determining receptors or signaling components. 2. **[TBC1D8 AS1](/details-gene/100506286) coordinates epithelial-immune crosstalk in the skin.** Its high expression in both keratinocytes/melanocytes and resident immune cells (e.g., dendritic cells, mast cells) suggests it may regulate a shared pathway involved in skin barrier defense, inflammatory responses, or pigmentation processes. ### Key Experimental Approach: To test the first hypothesis regarding T cell fate, one could perform a targeted knockdown of [TBC1D8 AS1](/details-gene/100506286) in human naive CD4+ and CD8+ T cells using antisense oligonucleotides (ASOs) or a CRISPR interference (CRISPRi) system. These modified cells would then be cultured under conditions that promote differentiation into memory CD8+, regulatory T cell, and various helper T cell subsets. The impact of the knockdown could be assessed by a combination of single-cell RNA sequencing to map transcriptomic changes in differentiation trajectories and flow cytometry to quantify the efficiency of lineage commitment. Functional assays, such as measuring the suppressive capacity of generated Tregs or the cytotoxic potential of CD8+ T cells, would directly link [TBC1D8 AS1](/details-gene/100506286) to cellular function. ### Therapeutic Potential: As a non-coding RNA, [TBC1D8 AS1](/details-gene/100506286) is a tractable target for RNA-based therapeutics like ASOs or siRNAs. Its specific expression profile makes it an attractive candidate for modulating immune responses in a targeted manner. For autoimmune conditions driven by pathogenic memory CD8+ T cells, selective inhibition of [TBC1D8 AS1](/details-gene/100506286) could serve to dampen their activity. Conversely, in oncology, disrupting its function in tumor-infiltrating [regulatory T cell](/details-cell/CL0000815) could potentially alleviate their immunosuppressive effects, thereby enhancing anti-tumor immunity. The therapeutic strategy would primarily involve inhibition, likely delivered via lipid nanoparticles or other advanced nucleic acid delivery platforms.