Details for: MOSPD1

Gene ID: 56180

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: MOSPD1

Ensembl ID: ENSG00000101928

Description: motile sperm domain containing 1

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • ciliated epithelial cell CL0000067
    CSI 7.2
    rCSI 6.33%
    PRS 91.08
  • pro-B cell CL0000826
    CSI 5.31
    rCSI 4.4%
    PRS 97.15
  • melanocyte CL0000148
    CSI 4.17
    rCSI 3.09%
    PRS 94.49
  • neural crest cell CL0011012
    CSI 3.78
    rCSI 2.99%
    PRS 93.52
  • epithelial cell of lung CL0000082
    CSI 3.53
    rCSI 2.93%
    PRS 97.32
  • neural progenitor cell CL0011020
    CSI 3.42
    rCSI 15.06%
    PRS 88.79
  • ependymal cell CL0000065
    CSI 3.2
    rCSI 6.49%
    PRS 84.97
  • erythrocyte CL0000232
    CSI 2.77
    rCSI 6.28%
    PRS 94.79
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.75
    rCSI 8.12%
    PRS 96.24
  • choroid plexus epithelial cell CL0000706
    CSI 2.75
    rCSI 4.5%
    PRS 92.76
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.74
    rCSI 3.16%
    PRS 91.73
  • stem cell CL0000034
    CSI 2.7
    rCSI 2.6%
    PRS 94.8
  • lung ciliated cell CL1000271
    CSI 2.66
    rCSI 3.08%
    PRS 93.04
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.43
    rCSI 3.11%
    PRS 94.19
  • peripheral nervous system neuron CL2000032
    CSI 2.22
    rCSI 3.02%
    PRS 92.99
  • basal cell CL0000646
    CSI 2.2
    rCSI 2.95%
    PRS 94.57
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.15
    rCSI 3.79%
    PRS 89.57
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 2.14
    rCSI 1.93%
    PRS 96.11
  • club cell CL0000158
    CSI 2.06
    rCSI 3.02%
    PRS 94.49
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.66
    rCSI 5.18%
    PRS 90.48
  • erythroid lineage cell CL0000764
    CSI 1.25
    rCSI 8.02%
    PRS 96.56

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [MOSPD1](/details-gene/56180) (Motile Sperm Domain Containing 1) is a protein-coding gene located on the X chromosome, implicated in fundamental cellular processes including cell differentiation and the regulation of transcription. Gene ontology annotations place the [MOSPD1](/details-gene/56180) protein in multiple cellular compartments, including the [nucleus](/details-cell/GO00005634), [cytoplasm](/details-cell/GO00005737), [endoplasmic reticulum membrane](/details-cell/GO00005789), and [Golgi membrane](/details-cell/GO0000139). **Overall**, expression data reveals its highest significance in [ciliated epithelial cell](/details-cell/CL0000067), early hematopoietic precursors like the [pro-B cell](/details-cell/CL0000826), and various cells of neural lineage such as the [melanocyte](/details-cell/CL0000148) and [neural crest cell](/details-cell/CL0011012). This diverse expression pattern suggests a role in establishing and maintaining specialized cell functions across multiple tissues. It has a clinical association recorded in OMIM ([300674](https://omim.org/entry/300674)). ## Cellular Roles and Expression Landscape The expression profile of [MOSPD1](/details-gene/56180) indicates a significant role in several distinct and highly specialized cell lineages. Its most prominent expression is found in the [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 7.20), suggesting a key function in mucosal tissues such as the airways. Beyond its epithelial role, [MOSPD1](/details-gene/56180) appears to be important in developmental contexts. It shows high significance in hematopoietic precursors, specifically the [pro-B cell](/details-cell/CL0000826) (CSI: 5.31), implying a potential function in early B-cell development. Furthermore, the gene is highly expressed across multiple cell types of the neural lineage, including [melanocyte](/details-cell/CL0000148) (CSI: 4.17), [neural crest cell](/details-cell/CL0011012) (CSI: 3.78), and [neural progenitor cell](/details-cell/CL0011020) (CSI: 3.42). This pattern points towards a role in neurogenesis and the differentiation of neural crest derivatives. This is further supported by research demonstrating its involvement in mesenchymal [stem cell](/details-cell/CL0000034) proliferation and differentiation [Link](https://doi.org/10.1002/stem.2102). The collective data suggests that [MOSPD1](/details-gene/56180) is not a ubiquitous housekeeping gene, but rather a specialized factor involved in the differentiation and function of specific cell types in epithelial, hematopoietic, and neural systems. ## Pathways and Molecular Function The functional annotations for [MOSPD1](/details-gene/56180) align with its observed expression pattern in progenitor and differentiated cells. Its association with [cell differentiation](/details-cell/GO000030154) is consistent with its high significance in developmental cells like [pro-B cell](/details-cell/CL0000826) and [neural progenitor cell](/details-cell/CL0011020). The gene's role in both [positive regulation of transcription by RNA polymerase II](/details-cell/GO00045944) and [negative regulation of transcription by RNA polymerase II](/details-cell/GO00000122) suggests it may function as a transcriptional modulator, fine-tuning gene expression programs that drive lineage commitment and cell identity. This regulatory function is supported by its annotated localization to the [nucleus](/details-cell/GO00005634). Concurrently, its presence in the [cytoplasm](/details-cell/GO00005737), [endoplasmic reticulum membrane](/details-cell/GO00005789), and [Golgi membrane](/details-cell/GO0000139) indicates that [MOSPD1](/details-gene/56180) might also participate in signaling pathways or protein-protein interactions outside of direct transcriptional control, potentially linking cytoplasmic signals to nuclear events. The broad molecular function annotation of [protein binding](/details-cell/GO00005515) supports its potential role as an adaptor or scaffold protein in multiple cellular compartments. ## Research Directions Based on its distinct expression pattern and functional annotations, the role of [MOSPD1](/details-gene/56180) warrants further investigation, particularly in the context of cell differentiation and specialized tissue function. **Proposed Hypotheses:** 1. Given its top-ranking significance in [ciliated epithelial cell](/details-cell/CL0000067), [MOSPD1](/details-gene/56180) is a critical transcriptional regulator required for ciliogenesis or the maintenance of ciliary function in tissues such as the lung epithelium. 2. The high expression of [MOSPD1](/details-gene/56180) in [pro-B cell](/details-cell/CL0000826) suggests it plays a key role in early B-lymphopoiesis, potentially by regulating the transcriptional network that governs the commitment and transition from the pro-B to the pre-B cell stage. 3. Based on its expression in [neural crest cell](/details-cell/CL0011012), [neural progenitor cell](/details-cell/CL0011020), and the neural crest-derived [melanocyte](/details-cell/CL0000148), [MOSPD1](/details-gene/56180) may be a master regulator of neural crest specification and subsequent differentiation into its diverse derivatives. **Experimental Approach:** To test the hypothesis that [MOSPD1](/details-gene/56180) is essential for ciliogenesis (Hypothesis 1), a targeted experiment could be performed. CRISPR-Cas9 could be used to knock out [MOSPD1](/details-gene/56180) in primary human bronchial epithelial cells. These cells would then be cultured at an air-liquid interface to induce differentiation into a mucociliary epithelium. The effects of the knockout could be assessed by immunofluorescence microscopy to visualize ciliary structures (e.g., staining for acetylated tubulin) and by high-speed video microscopy to measure ciliary beat frequency. RNA-sequencing of the knockout and control cells would identify downstream transcriptional targets of [MOSPD1](/details-gene/56180) and reveal its impact on the gene networks controlling ciliary assembly and function. **Therapeutic Potential:** The specific expression patterns of [MOSPD1](/details-gene/56180) suggest that its dysregulation could contribute to developmental disorders or cancers originating from epithelial, hematopoietic, or neural crest lineages. Given its intracellular localizations, including the nucleus, it is not an ideal target for antibody-based therapies. However, if it possesses a druggable protein-binding pocket, it could be a candidate for small molecule inhibitors or stabilizers. Further research into its precise molecular function is required to determine whether inhibition or activation would be the appropriate therapeutic strategy for diseases linked to its malfunction.

Genular Protein ID: 2032237992

Symbol: MSPD1_HUMAN

Name: Motile sperm domain-containing protein 1

UniProtKB Accession Codes:

Q9UJG1 B2RE62 D3DTG5 Q5H9C5 Q5H9C7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 6 Ensembl 3 GO 8 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 Pumba 1 RNAct 1 RefSeq 5 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TopDownProteomics 2 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15772651

Title: The DNA sequence of the human X chromosome.

PubMed ID: 15772651

DOI: 10.1038/nature03440

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 16959974

Title: The consensus coding sequences of human breast and colorectal cancers.

PubMed ID: 16959974

DOI: 10.1126/science.1133427

PubMed ID: 26175344

Title: A role for MOSPD1 in mesenchymal stem cell proliferation and differentiation.

PubMed ID: 26175344

DOI: 10.1002/stem.2102

Sequence Information:

  • Length: 213
  • Mass: 24086
  • Checksum: 2E0944F48058B47F
  • Sequence:
    • MHQQKRQPEL VEGNLPVFVF PTELIFYADD QSTHKQVLTL YNPYEFALKF KVLCTTPNKY 
VVVDAAGAVK PQCCVDIVIR HRDVRSCHYG VIDKFRLQVS EQSQRKALGR KEVVATLLPS 
AKEQQKEEEE KRLKEHLTES LFFEQSFQPE NRAVSSGPSL LTVFLGVVCI AALMLPTLGD 
VESLVPLYLH LSVNQKLVAA YILGLITMAI LRT