Details for: ZNF101

Gene ID: 94039

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF101

Ensembl ID: ENSG00000181896

Description: zinc finger protein 101

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • CD4-positive, alpha-beta thymocyte CL0000810
    CSI 5.02
    rCSI 4.02%
    PRS 94.91
  • mature alpha-beta T cell CL0000791
    CSI 3.83
    rCSI 13.85%
    PRS 98.01
  • effector memory CD8-positive, alpha-beta T cell CL0000913
    CSI 3.8
    rCSI 3.46%
    PRS 96.81
  • CD4-positive, alpha-beta memory T cell CL0000897
    CSI 3.8
    rCSI 2.73%
    PRS 97.32
  • double negative thymocyte CL0002489
    CSI 3.75
    rCSI 2.6%
    PRS 96.73
  • central memory CD4-positive, alpha-beta T cell CL0000904
    CSI 3.67
    rCSI 2.17%
    PRS 98.24
  • mature B cell CL0000785
    CSI 3
    rCSI 2.6%
    PRS 95.56
  • pro-B cell CL0000826
    CSI 2.82
    rCSI 2.33%
    PRS 91.06
  • mature T cell CL0002419
    CSI 2.79
    rCSI 2.17%
    PRS 97
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 2.76
    rCSI 2.81%
    PRS 94.68
  • naive T cell CL0000898
    CSI 2.64
    rCSI 1.83%
    PRS 97.51
  • central memory CD8-positive, alpha-beta T cell CL0000907
    CSI 2.51
    rCSI 1.69%
    PRS 97.24
  • class switched memory B cell CL0000972
    CSI 2.46
    rCSI 1.84%
    PRS 96.16
  • activated CD4-positive, alpha-beta T cell CL0000896
    CSI 2.41
    rCSI 2.23%
    PRS 97.39
  • T follicular helper cell CL0002038
    CSI 2.29
    rCSI 1.72%
    PRS 96.55
  • precursor B cell CL0000817
    CSI 2.22
    rCSI 1.95%
    PRS 93.72
  • ependymal cell CL0000065
    CSI 2.13
    rCSI 4.32%
    PRS 71.56
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 2.1
    rCSI 2.07%
    PRS 97.3
  • CD4-positive, alpha-beta cytotoxic T cell CL0000934
    CSI 1.96
    rCSI 2.7%
    PRS 97.51
  • effector memory CD4-positive, alpha-beta T cell CL0000905
    CSI 1.9
    rCSI 2.59%
    PRS 97.8

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

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Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF101](/details-gene/94039) is a protein-coding gene located on human chromosome 19p13.11. It encodes Zinc Finger Protein 101, a nuclear protein that functions as a sequence-specific, DNA-binding transcription factor. Gene ontology annotations indicate its involvement in the [regulation of transcription by rna polymerase ii](/details-cell/GO:0006357). **Overall**, expression data reveals that [ZNF101](/details-gene/94039) is a highly significant gene within the hematopoietic system, showing a particular enrichment in T-lymphocyte and B-lymphocyte lineages. Its expression pattern, which peaks in developing T cells within the thymus, suggests a fundamental role in lymphocyte differentiation and maturation. ## Cellular Roles and Expression Landscape The expression profile of [ZNF101](/details-gene/94039) strongly indicates a specialized function in the immune system, particularly within the T cell compartment. **Overall**, the gene shows its highest significance in [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810) (CSI: 5.02), pointing towards a critical role during T cell development. Its importance extends across various stages of T cell maturation and differentiation, as evidenced by high significance scores in [mature alpha-beta T cell](/details-cell/CL0000791), [effector memory CD8-positive, alpha-beta T cell](/details-cell/CL0000913), [CD4-positive, alpha-beta memory T cell](/details-cell/CL0000897), and earlier developmental stages like the [double negative thymocyte](/details-cell/CL0002489). While its role is most prominent in T cells, [ZNF101](/details-gene/94039) is also significantly expressed in B cell lineages, including [mature B cell](/details-cell/CL0000785) and [pro-B cell](/details-cell/CL0000826). The consistently high significance across both T and B lymphocyte populations suggests that [ZNF101](/details-gene/94039) may regulate core transcriptional programs essential for lymphocyte identity and function. ## Pathways and Molecular Function [ZNF101](/details-gene/94039) operates as a transcriptional regulator within the [nucleus](/details-cell/GO:0005634). Its molecular function is defined as a [dna-binding transcription factor activity, rna polymerase ii-specific](/details-cell/GO:0000981), which is mediated by its zinc finger domains responsible for [metal ion binding](/details-cell/GO:0046872) and sequence-specific DNA recognition. The protein contains a Kruppel-associated box (KRAB) domain, which strongly suggests that it functions as a transcriptional repressor ([Link](https://doi.org/10.1007/s00335-003-3022-0)). Functionally, [ZNF101](/details-gene/94039) is a component of fundamental cellular machinery governing gene expression, as annotated in Reactome pathways such as [Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and [Rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857). Its role as a likely repressor is consistent with its high expression during lymphocyte development, where it may be responsible for silencing inappropriate gene expression programs to ensure proper lineage commitment and maturation. ## Research Directions The specific expression pattern and presumed repressive function of [ZNF101](/details-gene/94039) in lymphocytes, particularly during T cell development, provide a basis for several testable hypotheses. 1. Given its peak expression in [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810), it is hypothesized that [ZNF101](/details-gene/94039) is a master repressor required for silencing non-T cell lineage genes or genes specific to earlier developmental stages (e.g., double-positive stage) to permit successful T cell positive selection and commitment. 2. The shared expression of [ZNF101](/details-gene/94039) in both T and B lymphocytes suggests it may regulate a common transcriptional network that maintains a general lymphocyte identity, possibly by repressing genes associated with myeloid or other hematopoietic lineages. To test the first hypothesis regarding its role in T cell development, a key experiment would be to generate a conditional knockout of [ZNF101](/details-gene/94039) in the murine T cell lineage (e.g., using a CD4-Cre driver). Subsequent analysis via multi-color flow cytometry of thymic and peripheral lymphoid organs would reveal any blocks or alterations in T cell maturation. This could be complemented with single-cell RNA-sequencing (scRNA-seq) of thymocytes from knockout and wild-type mice to identify the precise developmental stage affected and to uncover the downstream target genes that are de-repressed in the absence of [ZNF101](/details-gene/94039). As a lymphocyte-specific transcription factor, [ZNF101](/details-gene/94039) could be implicated in hematological malignancies or autoimmune diseases. Although transcription factors are challenging therapeutic targets, its high specificity to the immune compartment could make it a potential biomarker. If its loss of function contributes to lymphomagenesis by de-repressing oncogenes, strategies to restore its activity or target its downstream effectors could be explored. Conversely, if its aberrant overexpression drives an autoimmune phenotype, inhibiting its function or DNA binding could be a therapeutic goal.

Genular Protein ID: 2134469147

Symbol: ZN101_HUMAN

Name: Zinc finger protein 101

UniProtKB Accession Codes:

Q8IZC7 C9JU83 Q0VDG9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CDD 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 3 Ensembl 3 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 RNAct 1 Reactome 1 RefSeq 2 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14727140

Title: A novel Kruppel-associated box identified in a panel of mammalian zinc finger proteins.

PubMed ID: 14727140

DOI: 10.1007/s00335-003-3022-0

PubMed ID: 15057824

Title: The DNA sequence and biology of human chromosome 19.

PubMed ID: 15057824

DOI: 10.1038/nature02399

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 436
  • Mass: 50339
  • Checksum: 48EEA2EEA86212B3
  • Sequence:
    • MDSVAFEDVA VNFTQEEWAL LSPSQKNLYR DVTLETFRNL ASVGIQWKDQ DIENLYQNLG 
IKLRSLVERL CGRKEGNEHR ETFSQIPDCH LNKKSQTGVK PCKCSVCGKV FLRHSFLDRH 
MRAHAGHKRS ECGGEWRETP RKQKQHGKAS ISPSSGARRT VTPTRKRPYE CKVCGKAFNS 
PNLFQIHQRT HTGKRSYKCR EIVRAFTVSS FFRKHGKMHT GEKRYECKYC GKPIDYPSLF 
QIHVRTHTGE KPYKCKQCGK AFISAGYLRT HEIRSHALEK SHQCQECGKK LSCSSSLHRH 
ERTHSGGKLY ECQKCAKVFR CPTSLQAHER AHTGERPYEC NKCGKTFNYP SCFRRHKKTH 
SGEKPYECTR CGKAFGWCSS LRRHEMTHTG EKPFDCKQCG KVFTFSNYLR LHERTHLAGR 
SQCFGRRQGD HLSPGV