Details for: ZNF433 AS1

Gene ID: 101928464

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: ZNF433 AS1

Ensembl ID: ENSG00000219665

Description: ZNF433 and ZNF878 antisense RNA 1

Cell Significance Landscape

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • CD4-positive, alpha-beta thymocyte CL0000810
    CSI 5.7
    rCSI 4.57%
    PRS 96.4
  • CD1c-positive myeloid dendritic cell CL0002399
    CSI 4.02
    rCSI 4.86%
    PRS 99.07
  • CD8-positive, alpha-beta thymocyte CL0000811
    CSI 3.7
    rCSI 5.77%
    PRS 98.05
  • interneuron CL0000099
    CSI 3.59
    rCSI 7.2%
    PRS 95.81
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 3.47
    rCSI 2.67%
    PRS 99.08
  • mesothelial cell CL0000077
    CSI 3.33
    rCSI 13.01%
    PRS 91.42
  • neural crest cell CL0011012
    CSI 3.21
    rCSI 2.54%
    PRS 96.02
  • cerebellar granule cell CL0001031
    CSI 3.2
    rCSI 4.7%
    PRS 95.34
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 2.98
    rCSI 7.76%
    PRS 98.73
  • kidney connecting tubule epithelial cell CL1000768
    CSI 2.88
    rCSI 7.31%
    PRS 95.99
  • naive thymus-derived CD4-positive, alpha-beta T cell CL0000895
    CSI 2.87
    rCSI 3.61%
    PRS 99.45
  • retinal rod cell CL0000604
    CSI 2.66
    rCSI 4.69%
    PRS 95.56
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.55
    rCSI 2.95%
    PRS 94.6
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.51
    rCSI 3.22%
    PRS 96.51
  • Bergmann glial cell CL0000644
    CSI 2.48
    rCSI 3.39%
    PRS 94.35
  • mononuclear phagocyte CL0000113
    CSI 2.26
    rCSI 4.98%
    PRS 98.65
  • glioblast CL0000030
    CSI 2.06
    rCSI 3.29%
    PRS 94.75
  • radial glial cell CL0000681
    CSI 2.05
    rCSI 2.84%
    PRS 97.6
  • cardiac muscle cell CL0000746
    CSI 1.88
    rCSI 2.7%
    PRS 93.95
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 1.87
    rCSI 4.83%
    PRS 97.07
  • retinal cone cell CL0000573
    CSI 1.81
    rCSI 2.91%
    PRS 94.18
  • amacrine cell CL0000561
    CSI 1.76
    rCSI 5.11%
    PRS 94.06
  • alveolar macrophage CL0000583
    CSI 1.64
    rCSI 2.7%
    PRS 98.17
  • parietal epithelial cell CL1000452
    CSI 1.48
    rCSI 3.95%
    PRS 96.25
  • retinal ganglion cell CL0000740
    CSI 1.12
    rCSI 2.47%
    PRS 93.3
  • indirect pathway medium spiny neuron CL4023029
    CSI 0.29
    rCSI 7.05%
    PRS 91.09
  • direct pathway medium spiny neuron CL4023026
    CSI 0.28
    rCSI 6.66%
    PRS 91.35

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF433 AS1](/details-gene/101928464) is a non-coding antisense RNA located on chromosome 19p13.2. As an antisense transcript to the ZNF433 and ZNF878 genes, its primary function is likely post-transcriptional regulation of these targets. Analysis of its expression profile reveals a significant and notably diverse role across multiple biological systems. It is prominently expressed in developing immune cells within the thymus, such as [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810), and in various cell types of the central nervous system, including [interneuron](/details-cell/CL0000099). This dual-lineage significance suggests it may be involved in fundamental regulatory processes common to both hematopoietic and neural development, or that its function is contextually repurposed in different cellular environments. ## Cellular Roles and Expression Landscape The expression pattern of [ZNF433 AS1](/details-gene/101928464) highlights its importance in distinct and specialized cell populations. **Overall**, its expression landscape can be broadly categorized into roles within the immune and nervous systems. Within the immune system, [ZNF433 AS1](/details-gene/101928464) shows its highest significance in developing T lymphocytes, specifically [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810) (CSI: 5.70) and [CD8-positive, alpha-beta thymocyte](/details-cell/CL0000811) (CSI: 3.70). Its presence is also noted in mature innate immune cells, including [CD1c-positive myeloid dendritic cell](/details-cell/CL0002399) (CSI: 4.02) and [CD14-low, CD16-positive monocyte](/details-cell/CL0002396) (CSI: 3.47). This pattern suggests a potential regulatory role during T cell maturation and in the function of antigen-presenting cells and non-classical monocytes. Concurrently, [ZNF433 AS1](/details-gene/101928464) is a significant transcript in a diverse set of neural and developmental cell types. It is highly expressed in [interneuron](/details-cell/CL0000099) (CSI: 3.59), [neural crest cell](/details-cell/CL0011012) (CSI: 3.21), [cerebellar granule cell](/details-cell/CL0001031) (CSI: 3.20), and developing [neuroblast (sensu Vertebrata)](/details-cell/CL0000031) (CSI: 2.51). This expression profile is consistent with a role in neurogenesis, neuronal specification, and the function of specific neuronal circuits. The gene's activity in other specialized cells like [mesothelial cell](/details-cell/CL0000077) and [kidney connecting tubule epithelial cell](/details-cell/CL1000768) further underscores its broad, though context-specific, biological relevance. ## Pathways and Molecular Function As an antisense RNA, the primary molecular function of [ZNF433 AS1](/details-gene/101928464) is presumed to be the regulation of its sense-strand gene counterparts, ZNF433 and ZNF878, which are zinc finger proteins likely involved in transcriptional regulation. Antisense transcripts can modulate target gene expression through several mechanisms, including transcriptional interference, RNA masking that alters splicing or translation, or by directing epigenetic modifications. The elevated expression of [ZNF433 AS1](/details-gene/101928464) in specific cell types, such as thymocytes and neurons, suggests it may function to fine-tune the expression levels of these zinc finger proteins during critical developmental windows or in response to cellular signals. ## Research Directions The unique expression pattern of [ZNF433 AS1](/details-gene/101928464) across seemingly disparate cell lineages, combined with its identity as a regulatory non-coding RNA, prompts several avenues for future investigation. ### Proposed Hypotheses: 1. [ZNF433 AS1](/details-gene/101928464) acts as a critical modulator of T cell development by controlling the expression of ZNF433 and/or ZNF878, which may be required for specific stages of thymic selection. Its downregulation or upregulation at precise moments may be essential for the transition between developmental stages in [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810) and [CD8-positive, alpha-beta thymocyte](/details-cell/CL0000811). 2. In the central nervous system, [ZNF433 AS1](/details-gene/101928464) regulates the differentiation and functional maturation of specific neuronal subtypes, such as [interneuron](/details-cell/CL0000099) and [cerebellar granule cell](/details-cell/CL0001031), by fine-tuning the expression of its target transcription factors. 3. The expression of [ZNF433 AS1](/details-gene/101928464) is governed by distinct, lineage-specific enhancers and transcription factors, explaining its significant expression in both immune and neural cells without necessarily implying a shared functional mechanism. ### Suggested Experiment: To test the hypothesis that [ZNF433 AS1](/details-gene/101928464) is a key regulator of thymocyte development (Hypothesis 1), a loss-of-function study could be performed. Primary human thymocytes or a suitable hematopoietic stem cell line could be treated with targeted antisense oligonucleotides (ASOs) or a CRISPR interference (CRISPRi) system to specifically knock down [ZNF433 AS1](/details-gene/101928464) expression. The effect on T cell differentiation could be monitored in vitro using flow cytometry to quantify key populations (e.g., CD4/CD8 double-negative, double-positive, and single-positive cells). Parallel analysis via RNA-sequencing would reveal the direct impact on the expression of ZNF433 and ZNF878, as well as downstream gene networks associated with T cell fate decisions. ### Therapeutic Potential: As a non-coding RNA, [ZNF433 AS1](/details-gene/101928464) is a potential target for nucleic acid-based therapies such as ASOs or siRNAs. If its dysregulation is linked to autoimmune diseases or neurodevelopmental disorders, modulating its activity could have therapeutic benefit. However, its significant expression in both the immune and nervous systems presents a substantial challenge for systemic delivery, raising concerns about potential off-target effects. Therapeutic strategies would likely require highly targeted delivery methods or be most applicable to diseases where both systems are pathologically involved.