Details for: SYT8

Gene ID: 90019

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: SYT8

Ensembl ID: ENSG00000149043

Description: synaptotagmin 8

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • ciliated epithelial cell CL0000067
    CSI 7.93
    rCSI 6.97%
    PRS 89.78
  • keratinocyte CL0000312
    CSI 7.27
    rCSI 6.09%
    PRS 95.14
  • corneal epithelial cell CL0000575
    CSI 6.39
    rCSI 18.27%
    PRS 95.81
  • pancreatic acinar cell CL0002064
    CSI 5.23
    rCSI 6.95%
    PRS 96.82
  • secretory cell CL0000151
    CSI 5.21
    rCSI 5.44%
    PRS 94.88
  • lung ciliated cell CL1000271
    CSI 4.97
    rCSI 5.74%
    PRS 92.03
  • conjunctival epithelial cell CL1000432
    CSI 3.96
    rCSI 6.06%
    PRS 94.74
  • ciliated cell CL0000064
    CSI 3.76
    rCSI 6.09%
    PRS 90.71
  • epithelial cell of lower respiratory tract CL0002632
    CSI 3.67
    rCSI 2.85%
    PRS 96.91
  • stem cell CL0000034
    CSI 3.61
    rCSI 3.48%
    PRS 93.63
  • nasal mucosa goblet cell CL0002480
    CSI 3.53
    rCSI 4.1%
    PRS 95.09
  • respiratory basal cell CL0002633
    CSI 3.37
    rCSI 3.49%
    PRS 96.51
  • respiratory suprabasal cell CL4033048
    CSI 3.35
    rCSI 4.29%
    PRS 96.36
  • duct epithelial cell CL0000068
    CSI 3.35
    rCSI 4.9%
    PRS 97.42
  • extravillous trophoblast CL0008036
    CSI 2.64
    rCSI 3.27%
    PRS 94.41
  • epithelial cell CL0000066
    CSI 2.56
    rCSI 3.93%
    PRS 86.27
  • myoepithelial cell CL0000185
    CSI 2.4
    rCSI 6.08%
    PRS 96.82
  • multi-ciliated epithelial cell CL0005012
    CSI 2.4
    rCSI 2.4%
    PRS 91.8
  • basal cell CL0000646
    CSI 2.35
    rCSI 3.15%
    PRS 93.65
  • respiratory hillock cell CL4030023
    CSI 2.16
    rCSI 3.85%
    PRS 97.12
  • pancreatic ductal cell CL0002079
    CSI 2.13
    rCSI 4.14%
    PRS 96.11
  • fallopian tube secretory epithelial cell CL4030006
    CSI 1.94
    rCSI 1.87%
    PRS 94.61
  • club cell CL0000158
    CSI 1.86
    rCSI 2.72%
    PRS 93.21
  • tracheal goblet cell CL1000329
    CSI 1.83
    rCSI 3.98%
    PRS 96.12
  • placental villous trophoblast CL2000060
    CSI 1.73
    rCSI 2.67%
    PRS 94.33
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 1.32
    rCSI 3.02%
    PRS 90.11
  • basal cell of epithelium of trachea CL1000348
    CSI 0.82
    rCSI 5.8%
    PRS 95.61

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [SYT8](/details-gene/90019) (synaptotagmin 8) is a protein-coding gene located on chromosome 11p15.5. As a member of the synaptotagmin family, it functions as a calcium sensor involved in regulating vesicle-mediated transport and exocytosis. Its molecular functions include [calcium-dependent phospholipid binding](/details-go/GO:0005544) and [SNARE binding](/details-go/GO:0000149), positioning it as a key regulator of membrane fusion events. While the synaptotagmin family is well-known for its role in neuronal synapses, expression data suggests that **overall**, [SYT8](/details-gene/90019) has a prominent role in non-neuronal cells. It is a highly significant marker in various epithelial cell types, particularly [ciliated epithelial cell](/details-cell/CL0000067), [keratinocyte](/details-cell/CL0000312), and secretory cells such as [pancreatic acinar cell](/details-cell/CL0002064), indicating a broad function in specialized secretion and membrane trafficking across diverse tissues. ## Cellular Roles and Expression Landscape The expression profile of [SYT8](/details-gene/90019) highlights its significant role in specialized epithelial and secretory cell populations. **Overall**, the gene shows its highest significance in cells involved in barrier function, motility, and secretion. The top-ranked cell types can be functionally grouped: * **Ciliated Epithelia:** [SYT8](/details-gene/90019) is a defining marker for [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 7.93), [lung ciliated cell](/details-cell/CL1000271) (CSI: 4.97), and [ciliated cell](/details-cell/CL0000064) (CSI: 3.76). This strong association suggests a potential role in processes unique to these cells, such as the assembly or maintenance of the ciliary membrane or the regulation of periciliary vesicle transport. * **Secretory Epithelia:** The gene is highly significant in [pancreatic acinar cell](/details-cell/CL0002064) (CSI: 5.23), [secretory cell](/details-cell/CL0000151) (CSI: 5.21), and [nasal mucosa goblet cell](/details-cell/CL0002480) (CSI: 3.53). This pattern is consistent with its function in calcium-dependent exocytosis, suggesting it may mediate the release of enzymes, mucus, and other secreted products in these tissues. * **Stratified and Barrier Epithelia:** High significance in [keratinocyte](/details-cell/CL0000312) (CSI: 7.27) and [corneal epithelial cell](/details-cell/CL0000575) (CSI: 6.39) implies a role in maintaining epithelial integrity, possibly by regulating the trafficking of lipids or proteins essential for barrier formation. * **Progenitor Cells:** [SYT8](/details-gene/90019) also shows significance in [stem cell](/details-cell/CL0000034) (CSI: 3.61) and [respiratory basal cell](/details-cell/CL0002633) (CSI: 3.37), suggesting a potential function in the processes of cellular differentiation and tissue regeneration. Notably, despite its name, classical neuronal cell types are absent from the top markers, indicating that its primary physiological impact may be outside of the central nervous system's rapid synaptic transmission, focusing instead on regulated secretion in other tissues. ## Pathways and Molecular Function The functional annotations for [SYT8](/details-gene/90019) are highly consistent with its observed expression pattern in secretory and epithelial cells. It is centrally involved in [vesicle-mediated transport](/details-go/GO:0016192) and membrane trafficking events. As a [calcium ion sensor](/details-go/GO:0061891), [SYT8](/details-gene/90019) is annotated with the biological process of [regulation of calcium ion-dependent exocytosis](/details-go/GO:0017158). This function directly explains its high significance in cells that perform regulated secretion, such as the [pancreatic acinar cell](/details-cell/CL0002064), which releases digestive enzymes from [dense core granule](/details-go/GO:0031045)s in a calcium-dependent manner. Its localization to the [exocytic vesicle](/details-go/GO:0070382), [synaptic vesicle membrane](/details-go/GO:0030672), and [plasma membrane](/details-go/GO:0005886) further supports its role at the final steps of vesicle fusion. Reactome pathway analysis places [SYT8](/details-gene/90019) within the broader contexts of [Vesicle-mediated transport](/details-reactome/R-HSA-5653656) and [Membrane Trafficking](/details-reactome/R-HSA-199991). Its specific involvement in [Clathrin-mediated endocytosis](/details-reactome/R-HSA-8856828) suggests that in addition to regulating exocytosis, it may also participate in the compensatory retrieval of vesicle membranes following fusion, a critical process for maintaining cellular homeostasis. ## Research Directions The available data highlights [SYT8](/details-gene/90019) as a key regulator of specialized membrane trafficking in non-neuronal tissues, opening several avenues for future investigation. **Proposed Testable Hypotheses:** 1. **Hypothesis 1:** Given its high significance in [ciliated epithelial cell](/details-cell/CL0000067) and its role in vesicle transport, [SYT8](/details-gene/90019) is essential for ciliogenesis or the maintenance of ciliary function by regulating the transport of proteins and lipids to the ciliary membrane. 2. **Hypothesis 2:** In [pancreatic acinar cell](/details-cell/CL0002064), [SYT8](/details-gene/90019) acts as the primary calcium sensor that triggers the fusion of zymogen granules with the apical plasma membrane during secretagogue-induced digestive enzyme secretion. 3. **Hypothesis 3:** The high expression of [SYT8](/details-gene/90019) in [keratinocyte](/details-cell/CL0000312) is critical for skin barrier formation, where it mediates the calcium-dependent exocytosis of lamellar bodies containing lipids and structural proteins. **Suggested Experimental Approach:** To test Hypothesis 2, one could utilize a pancreatic organoid model derived from human stem cells or the AR42J rat acinar cell line. * **Experiment:** Generate a stable [SYT8](/details-gene/90019) knockout cell line using CRISPR-Cas9 gene editing. * **Procedure:** Culture both wild-type and knockout cells and stimulate them with a secretagogue like cholecystokinin (CCK) to induce a calcium influx. * **Analysis:** Measure the secretion of the digestive enzyme amylase into the supernatant using a colorimetric activity assay. Concurrently, use total internal reflection fluorescence (TIRF) microscopy to visualize the docking and fusion of fluorescently-labeled zymogen granules at the plasma membrane in real-time. * **Expected Outcome:** A significant reduction in both amylase secretion and granule fusion events in the [SYT8](/details-gene/90019) knockout cells upon stimulation would confirm its essential role in regulated exocytosis in this cell type. **Therapeutic Potential:** The specific role of [SYT8](/details-gene/90019) in regulated secretion makes it a potential therapeutic target in diseases characterized by exocrine dysfunction. For example, in conditions involving excessive secretion, such as certain forms of acute pancreatitis, a targeted inhibitor of [SYT8](/details-gene/90019) could potentially reduce premature enzyme release and mitigate tissue damage. Conversely, in diseases of insufficient secretion, such as Sjögren's syndrome or specific subtypes of cystic fibrosis, strategies to enhance [SYT8](/details-gene/90019) function or expression might restore glandular activity. However, its expression across multiple vital epithelial tissues suggests that any therapeutic intervention would require highly targeted delivery to avoid off-target effects.

Genular Protein ID: 3466162017

Symbol: SYT8_HUMAN

Name: Synaptotagmin-8

UniProtKB Accession Codes:

Q8NBV8 A6NCR4 A6NFJ4 Q9NSV9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 4 Ensembl 2 ExpressionAtlas 1 GO 13 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 InterPro 3 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PIR 1 PRINTS 1 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 3 RNAct 1 Reactome 2 RefSeq 3 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 neXtProt 1

Citations:

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

Sequence Information:

  • Length: 387
  • Mass: 42433
  • Checksum: 414115987101650D
  • Sequence:
    • MGHPPVSPSA PAPAGTTAIP GLIPDLVAGT PWPRWALIAG ALAAGVLLVS CLLCAACCCC 
RRHRKKPRDK ESVGLGSARG TTTTHLVQPD VDGLESSPGD AQQWGCLQLS LEFDFGSQEI 
RVGLRQAADL RPGGTVDPYA RVSVSTQAGH RHETKVHRGT LCPVFDETCC FHIPQAELPG 
ATLQVQLFNF KRFSGHEPLG ELRLPLGTVD LQHVLEHWYL LGPPAATQPE QVGELCFSLR 
YVPSSGRLTV VVLEARGLRP GLAEPYVKVQ LMLNQRKWKK RKTATKKGTA APYFNEAFTF 
LVPFSQVQNV DLVLAVWDRS LPLRTEPVGK VHLGARASGQ PLQHWADMLA HARRPIAQRH 
PLRPAREVDR MLALQPRLRL RLPLPHS