Details for: ANKRD34C AS1

Gene ID: 729911

Gene Type:  ncRNA (Non-coding RNA)  - A functional RNA molecule that is transcribed from DNA but not translated into a protein. Includes classes like miRNA and lncRNA.

Symbol: ANKRD34C AS1

Ensembl ID: ENSG00000259234

Description: ANKRD34C antisense RNA 1

Cell Significance Landscape

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • pvalb GABAergic cortical interneuron CL4023018
    CSI 55.74
    rCSI 69.35%
    PRS 98.33
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 30.93
    rCSI 54.62%
    PRS 98.59
  • sst GABAergic cortical interneuron CL4023017
    CSI 24.6
    rCSI 31.71%
    PRS 98.86
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 23.62
    rCSI 57.42%
    PRS 97.96
  • sncg GABAergic cortical interneuron CL4023015
    CSI 21.01
    rCSI 33.79%
    PRS 98.43
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 18.93
    rCSI 59.2%
    PRS 98.69
  • neuron CL0000540
    CSI 18.73
    rCSI 49.88%
    PRS 97.55
  • L6b glutamatergic cortical neuron CL4023038
    CSI 18.41
    rCSI 57.53%
    PRS 98.54
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 17.97
    rCSI 64.65%
    PRS 98.25
  • inhibitory interneuron CL0000498
    CSI 17.72
    rCSI 40.91%
    PRS 98.67
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 17.64
    rCSI 42.19%
    PRS 98.06
  • glutamatergic neuron CL0000679
    CSI 17.55
    rCSI 36.07%
    PRS 97.22
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 17.39
    rCSI 29.19%
    PRS 98.68
  • cerebellar granule cell CL0001031
    CSI 15.19
    rCSI 22.33%
    PRS 98.88
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 14.78
    rCSI 55.87%
    PRS 98.22
  • GABAergic neuron CL0000617
    CSI 11.61
    rCSI 38.91%
    PRS 97.01
  • amacrine cell CL0000561
    CSI 11.13
    rCSI 32.27%
    PRS 98.72
  • VIP GABAergic cortical interneuron CL4023016
    CSI 10.12
    rCSI 12.09%
    PRS 98.56
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 8.99
    rCSI 52.9%
    PRS 98.35
  • retinal ganglion cell CL0000740
    CSI 7.98
    rCSI 17.63%
    PRS 98.54
  • L2/3 intratelencephalic projecting glutamatergic neuron CL4030059
    CSI 6.74
    rCSI 14.62%
    PRS 97.74
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 5.95
    rCSI 19.57%
    PRS 97.79
  • direct pathway medium spiny neuron CL4023026
    CSI 2.83
    rCSI 67.85%
    PRS 97.54
  • indirect pathway medium spiny neuron CL4023029
    CSI 2.8
    rCSI 67.63%
    PRS 97.53
  • ON parasol ganglion cell CL4033052
    CSI 1.92
    rCSI 27.2%
    PRS 98.59
  • ON midget ganglion cell CL4033046
    CSI 1.01
    rCSI 20.54%
    PRS 98.45

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ANKRD34C AS1](/details-gene/729911) is a long non-coding RNA (lncRNA) located on human chromosome 15q25.1. Expression data indicates that this gene is a highly specific marker for neuronal populations within the central nervous system. Its significance is particularly pronounced in [pvalb GABAergic cortical interneuron](/details-cell/CL4023018), where it shows the highest level of specific expression. The gene is also significantly expressed across a range of other inhibitory and excitatory neuron subtypes, suggesting a specialized role in establishing or maintaining neuronal identity and function, particularly within cortical circuits. ## Cellular Roles and Expression Landscape The expression profile of [ANKRD34C AS1](/details-gene/729911) is almost exclusively restricted to neuronal cells, highlighting its potential importance in the nervous system. The **Overall** expression data reveals a clear hierarchy of significance across different neuronal subtypes. * **Primary Association with Inhibitory Interneurons:** The gene's most striking feature is its exceptionally high significance in [pvalb GABAergic cortical interneuron](/details-cell/CL4023018) (CSI: 55.74), suggesting it may be a key regulator of this cell's identity or function. Its role in inhibitory circuitry is further supported by its significant expression in other interneuron populations, including those derived from the [caudal ganglionic eminence](/details-cell/CL4023064), [sst GABAergic cortical interneuron](/details-cell/CL4023017), and [sncg GABAergic cortical interneuron](/details-cell/CL4023015). * **Broad Role in Excitatory Neurons:** While its peak expression is in inhibitory neurons, [ANKRD34C AS1](/details-gene/729911) is also a significant marker for various excitatory [glutamatergic neuron](/details-cell/CL0000679) populations. This includes diverse cortical layer neurons such as [L2/3-6 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4023040), [L6b glutamatergic cortical neuron](/details-cell/CL4023038), and [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041). This expression pattern across both major classes of cortical neurons suggests [ANKRD34C AS1](/details-gene/729911) may be involved in fundamental neuronal processes or in coordinating the balance between excitation and inhibition in the cortex. The highly restricted expression implies a specialized function in neuronal development, synaptic regulation, or circuit maintenance, with little to no role in non-neuronal tissues. ## Pathways and Molecular Function The specific molecular functions and biological pathways involving [ANKRD34C AS1](/details-gene/729911) have not yet been well characterized. As a long non-coding antisense RNA, it is hypothesized to exert its function by regulating the expression of other genes. This could occur through various mechanisms, including transcriptional modulation of its sense-strand counterpart (*ANKRD34C*), guiding chromatin-modifying complexes to specific genomic loci, or by altering the stability or translation of target mRNAs. Elucidating its precise molecular interactions and downstream targets is a critical area for future research. ## Research Directions The neuron-specific expression profile of [ANKRD34C AS1](/details-gene/729911), especially its enrichment in distinct interneuron subtypes, opens up several avenues for investigation. **Proposed Hypotheses:** 1. **Regulation of PVALB Interneuron Identity:** Given its paramount significance in [pvalb GABAergic cortical interneuron](/details-cell/CL4023018), it is hypothesized that [ANKRD34C AS1](/details-gene/729911) is a critical factor in the differentiation, maturation, or functional maintenance of this cell type. Its knockdown may disrupt the expression of key genes responsible for the fast-spiking phenotype characteristic of these neurons. 2. **Modulation of Cortical Excitatory/Inhibitory Balance:** The gene's presence in both principal glutamatergic neurons and various GABAergic interneurons suggests a potential role in regulating the excitatory/inhibitory (E/I) balance within cortical microcircuits. Dysregulation of [ANKRD34C AS1](/details-gene/729911) could therefore contribute to the pathophysiology of neurological disorders characterized by E/I imbalance, such as epilepsy or schizophrenia. **Suggested Experimental Approach:** To test the hypothesis that [ANKRD34C AS1](/details-gene/729911) is crucial for [pvalb GABAergic cortical interneuron](/details-cell/CL4023018) function, a targeted knockdown experiment in a mouse model would be highly informative. This could be achieved by stereotactic injection of antisense oligonucleotides (ASOs) designed against *Ankrd34c as1* into the cortex. The functional consequences could be assessed using *in vivo* two-photon calcium imaging to monitor neuronal activity during behavior, combined with *ex vivo* electrophysiological recordings to measure changes in intrinsic firing properties and synaptic connectivity of PVALB-positive neurons. Concurrently, single-cell RNA sequencing could be performed on sorted interneurons to identify the downstream gene expression programs regulated by this lncRNA. **Therapeutic Potential:** The high neuronal specificity of [ANKRD34C AS1](/details-gene/729911) makes it an intriguing, albeit challenging, therapeutic target. As dysfunction of [pvalb GABAergic cortical interneuron](/details-cell/CL4023018) is strongly implicated in schizophrenia and other neurodevelopmental disorders, modulating [ANKRD34C AS1](/details-gene/729911) could offer a novel therapeutic strategy. RNA-based therapies, such as ASOs, could provide a means to precisely manipulate its expression. Such an approach would likely involve a strategy of inhibition or replacement to restore normal circuit function. However, the significant hurdle of delivering these nucleic acid-based drugs across the blood-brain barrier would need to be overcome.