Details for: ZNF677

Gene ID: 342926

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF677

Ensembl ID: ENSG00000197928

Description: zinc finger protein 677

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • mature B cell CL0000785
    CSI 3.82
    rCSI 3.32%
    PRS 98.45
  • interneuron CL0000099
    CSI 3.67
    rCSI 7.37%
    PRS 92.54
  • pro-B cell CL0000826
    CSI 3.67
    rCSI 3.04%
    PRS 96.47
  • progenitor cell CL0011026
    CSI 3.52
    rCSI 7.49%
    PRS 91.23
  • ON-bipolar cell CL0000749
    CSI 3.4
    rCSI 5.05%
    PRS 94.34
  • interstitial cell of Cajal CL0002088
    CSI 3.35
    rCSI 4.27%
    PRS 97.71
  • basal cell of epidermis CL0002187
    CSI 3.25
    rCSI 5.75%
    PRS 72.79
  • neural crest cell CL0011012
    CSI 3.17
    rCSI 2.51%
    PRS 92.16
  • renal alpha-intercalated cell CL0005011
    CSI 3.16
    rCSI 4.23%
    PRS 97.02
  • early lymphoid progenitor CL0000936
    CSI 3.07
    rCSI 2.69%
    PRS 97.5
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 3.06
    rCSI 2.76%
    PRS 95.1
  • B cell CL0000236
    CSI 2.87
    rCSI 3.85%
    PRS 93.88
  • mesodermal cell CL0000222
    CSI 2.86
    rCSI 3.44%
    PRS 95.61
  • ciliated epithelial cell CL0000067
    CSI 2.73
    rCSI 2.4%
    PRS 89.9
  • rod bipolar cell CL0000751
    CSI 2.69
    rCSI 4.83%
    PRS 92.39
  • hematopoietic stem cell CL0000037
    CSI 2.66
    rCSI 1.77%
    PRS 96.49
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.65
    rCSI 3.06%
    PRS 90.54
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.59
    rCSI 4.57%
    PRS 87.85
  • keratinocyte CL0000312
    CSI 2.53
    rCSI 2.12%
    PRS 95.2
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.53
    rCSI 7.47%
    PRS 95.46
  • intestinal epithelial cell CL0002563
    CSI 2.43
    rCSI 2.54%
    PRS 94.11
  • enteric smooth muscle cell CL0002504
    CSI 2.33
    rCSI 3.32%
    PRS 95.93
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.32
    rCSI 2.98%
    PRS 93.19
  • respiratory basal cell CL0002633
    CSI 2.24
    rCSI 2.32%
    PRS 96.57
  • suprabasal keratinocyte CL4033013
    CSI 2.17
    rCSI 3.54%
    PRS 72.84
  • radial glial cell CL0000681
    CSI 2.06
    rCSI 2.87%
    PRS 94.73
  • retina horizontal cell CL0000745
    CSI 2.06
    rCSI 3.14%
    PRS 93.65
  • chondrocyte CL0000138
    CSI 1.96
    rCSI 3.12%
    PRS 92.91
  • peripheral nervous system neuron CL2000032
    CSI 1.93
    rCSI 2.64%
    PRS 91.78
  • club cell CL0000158
    CSI 1.9
    rCSI 2.79%
    PRS 93.29
  • glioblast CL0000030
    CSI 1.81
    rCSI 2.88%
    PRS 90.72
  • retinal cone cell CL0000573
    CSI 1.75
    rCSI 2.81%
    PRS 90.27
  • vascular associated smooth muscle cell CL0000359
    CSI 1.59
    rCSI 5.14%
    PRS 95.07
  • pulmonary artery endothelial cell CL1001568
    CSI 1.57
    rCSI 2.13%
    PRS 97.61
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.44
    rCSI 4.51%
    PRS 89.09
  • mesenchymal cell CL0008019
    CSI 1.3
    rCSI 3.31%
    PRS 93.39
  • forebrain radial glial cell CL0013000
    CSI 1.21
    rCSI 3.89%
    PRS 95.46
  • CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203
    CSI 1.21
    rCSI 1.47%
    PRS 79.93
  • helper T cell CL0000912
    CSI 1.17
    rCSI 1.65%
    PRS 90.97
  • melanocyte of skin CL1000458
    CSI 1.09
    rCSI 1.49%
    PRS 72.76

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary Analyzed for its specificity using the CSI (Z-SCORE), [ZNF677](/gene/342926) is a zinc finger protein that functions as a transcription factor. The data suggests it is not a defining marker for any single cell type but is instead expressed broadly across diverse cellular lineages. Its molecular function is consistently associated with the regulation of transcription, pointing towards a role in general or basal gene expression machinery rather than cell-type-specific identity programs. ## Cellular Roles and Expression Landscape The expression profile of [ZNF677](/gene/342926), when evaluated for cellular specificity, indicates a lack of enrichment in any particular cell population. In the **Overall** context, the CSI (Z-SCORE) is 0.00 with non-significant p-values (p > 0.5) across a wide array of top-expressing cells. This includes cells from distinct developmental origins such as hematopoietic lineages like [mature B cells](/details-cell/CL0000785) and [pro-B cells](/details-cell/CL0000826), neuronal cells including [interneurons](/details-cell/CL0000099), and epithelial cells like [basal cells of the epidermis](/details-cell/CL0002187). This lack of a significant specificity score for any cell type suggests that [ZNF677](/gene/342926) does not serve as a unique molecular identifier. Instead, its presence across disparate cell types is consistent with a housekeeping or general regulatory function. While its percentile rank scores (PRS) are often high (e.g., 98.45% in [mature B cells](/details-cell/CL0000785)), the zero Z-score clarifies that this expression level is not significantly different from its average expression across all other cell types. This pattern reinforces its role as a broadly utilized component of the cellular machinery rather than a driver of specialized cellular identity. ## Pathways and Molecular Function The functional annotations for [ZNF677](/gene/342926) align well with its observed broad but non-specific expression pattern. As a zinc finger protein, its primary molecular functions are related to DNA binding and transcriptional regulation. Gene Ontology terms confirm its role in '[Dna-binding transcription factor activity, rna polymerase ii-specific](/details-go/GO:0000981)' and '[Regulation of transcription by rna polymerase ii](/details-go/GO:0006357)', with a predicted localization to the '[Nucleus](/details-go/GO:0005634)'. Furthermore, its involvement in fundamental Reactome pathways such as '[Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160)' and '[Rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857)' is consistent with a role in basal cellular processes required by many different cell types. This molecular profile provides a strong mechanistic basis for the observed ubiquitous expression, suggesting [ZNF677](/gene/342926) is part of the core transcriptional apparatus, as initially identified in the Mammalian Gene Collection project ([PubMed: 15489334](https://pubmed.ncbi.nlm.nih.gov/15489334/)). ## Research Directions The current data portrays [ZNF677](/gene/342926) as a general transcription factor. However, this broad-strokes view may mask more nuanced, context-dependent functions. Future research should focus on elucidating its specific targets and regulatory logic under various physiological and pathological conditions. ### Testable Hypotheses: 1. **Hypothesis:** [ZNF677](/gene/342926) acts as a regulator of a core set of housekeeping genes essential for basal cell survival and function across multiple lineages. * **Experimental Approach:** Perform Chromatin Immunoprecipitation sequencing (ChIP-seq) for ZNF677 in at least three distinct cell lines (e.g., a B-lymphocyte, a neuronal, and an epithelial line). Identification of a common set of promoters bound by ZNF677 across all cell types would support this hypothesis. This could be validated by observing concordant changes in the expression of these putative target genes following siRNA-mediated knockdown of [ZNF677](/gene/342926). 2. **Hypothesis:** The regulatory role of [ZNF677](/gene/342926) becomes highly specific and critical during cellular stress, differentiation, or disease, where it may be dynamically recruited to specific gene loci. * **Experimental Approach:** Utilize a cellular model of differentiation, such as the transition from a [pro-B cell](/details-cell/CL0000826) to a [mature B cell](/details-cell/CL0000785). Conduct time-resolved single-cell RNA-seq and ATAC-seq during this process to determine if [ZNF677](/gene/342926) expression or the accessibility of its binding motifs changes dynamically. A shift in its expression pattern or co-expression with lineage-specific factors would suggest a context-dependent role. 3. **Hypothesis:** [ZNF677](/gene/342926) functions as a co-factor that modulates the activity of lineage-defining master transcription factors, rather than acting as a primary driver of cell identity itself. * **Experimental Approach:** In a relevant cell type like a [mature B cell](/details-cell/CL0000785), perform co-immunoprecipitation of endogenous ZNF677 followed by mass spectrometry (Co-IP/MS) to identify interacting proteins. If key B-cell regulators (e.g., PAX5, EBF1) are identified, their functional interaction can be validated using luciferase reporter assays to test if ZNF677 enhances or represses their activity on known target gene promoters. ### Therapeutic Potential: Given its broad expression profile, systemically targeting [ZNF677](/gene/342926) would likely cause significant off-target toxicity. However, if its function is found to be contextually essential for a specific disease state, such as a malignancy or an inflammatory condition, it could represent a viable therapeutic target. For instance, if it is discovered to be a critical co-factor for an oncogenic transcription factor, developing inhibitors of the specific ZNF677-partner interaction could offer a targeted therapeutic strategy. Further investigation into its context-specific roles is required to assess this potential.

Genular Protein ID: 2070147372

Symbol: ZN677_HUMAN

Name: Zinc finger protein 677

UniProtKB Accession Codes:

Q86XU0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 1 Ensembl 2 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 3 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 584
  • Mass: 67996
  • Checksum: F111E49F528CB29F
  • Sequence:
    • MALSQGLFTF KDVAIEFSQE EWECLDPAQR ALYRDVMLEN YRNLLSLDED NIPPEDDISV 
GFTSKGLSPK ENNKEELYHL VILERKESHG INNFDLKEVW ENMPKFDSLW DYDVKNYKGM 
PLTCNKNLTH RKDQQHNKSS IHFSLKQSVS IRDSAHQYFI HDKPFIRNLL KLKNNIRYAG 
NKYVKCFENK IGLSLQAQLA ELQRFQTGEK MYECNPVEKS INSSSVSPLP PCVKNICNKY 
RKILKYPLLH TQYGRTHIRE KSYKCNDCGK AFSKSSNLTN HQRIHSGQRP YKCNECGKAF 
NQCSNLTRHQ RVHTGEKPYQ CNICGKVCSQ NSNLASHQRM HTGEKPYKCN ECGKAFIQRS 
HLWGHERIHT GEKPYKCNEC DKAFAERSSL TQHKRIHTGE KPYICNECGK AFKQCSHLTR 
HQNIHPGEKP HKCNVCGRAF IQSSSLVEHQ RIHTGEKPYK CNKCDKAFIK RSHLWGHQRT 
HTGEKPYKCT ECGKAFTERS NLTQHKKIHT GEKPYKCTEC GKAFTQFANL TRHQKIHIEK 
KHCKHNIHGN ALFQSSNLGD HQKSYNREKH IKYNETKIKY SSCT