Details for: MOSPD3

Gene ID: 64598

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: MOSPD3

Ensembl ID: ENSG00000106330

Description: motile sperm domain containing 3

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • interneuron CL0000099
    CSI 8.65
    rCSI 17.37%
    PRS 83.66
  • mesodermal cell CL0000222
    CSI 6.12
    rCSI 7.35%
    PRS 88.77
  • intermediate monocyte CL0002393
    CSI 5.48
    rCSI 8.28%
    PRS 93.78
  • erythrocyte CL0000232
    CSI 4.94
    rCSI 11.21%
    PRS 88.36
  • epithelial cell of lung CL0000082
    CSI 4.2
    rCSI 3.48%
    PRS 90.65
  • vascular associated smooth muscle cell CL0000359
    CSI 4.01
    rCSI 13.02%
    PRS 88.04
  • neural progenitor cell CL0011020
    CSI 3.85
    rCSI 16.92%
    PRS 79.5
  • group 3 innate lymphoid cell CL0001071
    CSI 3.73
    rCSI 2.8%
    PRS 93.26
  • hematopoietic stem cell CL0000037
    CSI 3.38
    rCSI 2.25%
    PRS 91.33
  • epithelial cell of lower respiratory tract CL0002632
    CSI 3.25
    rCSI 2.52%
    PRS 92.43
  • fibroblast of lung CL0002553
    CSI 3.23
    rCSI 3.01%
    PRS 91.08
  • lung macrophage CL1001603
    CSI 3.22
    rCSI 7.19%
    PRS 94.23
  • duct epithelial cell CL0000068
    CSI 3.21
    rCSI 4.7%
    PRS 93.18
  • interstitial cell of Cajal CL0002088
    CSI 3.21
    rCSI 4.08%
    PRS 93.44
  • neural crest cell CL0011012
    CSI 3.19
    rCSI 2.52%
    PRS 82.76
  • ciliated cell CL0000064
    CSI 3.16
    rCSI 5.12%
    PRS 84.08
  • secretory cell CL0000151
    CSI 2.95
    rCSI 3.08%
    PRS 88.75
  • ionocyte CL0005006
    CSI 2.93
    rCSI 3.13%
    PRS 91.28
  • early lymphoid progenitor CL0000936
    CSI 2.92
    rCSI 2.56%
    PRS 93.08
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 2.86
    rCSI 2.81%
    PRS 97.4
  • myeloid leukocyte CL0000766
    CSI 2.83
    rCSI 2.61%
    PRS 91
  • CD4-positive, alpha-beta cytotoxic T cell CL0000934
    CSI 2.73
    rCSI 3.75%
    PRS 97.65
  • skin fibroblast CL0002620
    CSI 2.68
    rCSI 2.31%
    PRS 89.38
  • enteric smooth muscle cell CL0002504
    CSI 2.68
    rCSI 3.82%
    PRS 90.09
  • granulocyte monocyte progenitor cell CL0000557
    CSI 2.59
    rCSI 2.24%
    PRS 91.99
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.56
    rCSI 4.52%
    PRS 76.12
  • L6b glutamatergic cortical neuron CL4023038
    CSI 2.54
    rCSI 7.94%
    PRS 78.09
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.53
    rCSI 7.47%
    PRS 90.06
  • peripheral nervous system neuron CL2000032
    CSI 2.46
    rCSI 3.36%
    PRS 83.56
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 2.44
    rCSI 2.82%
    PRS 82.79
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 2.38
    rCSI 2.15%
    PRS 88.66
  • stem cell CL0000034
    CSI 2.28
    rCSI 2.2%
    PRS 85.82
  • multi-ciliated epithelial cell CL0005012
    CSI 2.27
    rCSI 2.27%
    PRS 84.23
  • CD14-positive monocyte CL0001054
    CSI 2.26
    rCSI 2.82%
    PRS 95.36
  • sst GABAergic cortical interneuron CL4023017
    CSI 2.2
    rCSI 2.84%
    PRS 77.8
  • ciliated epithelial cell CL0000067
    CSI 2.14
    rCSI 1.89%
    PRS 81.16
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.13
    rCSI 2.74%
    PRS 86.48
  • mesenchymal cell CL0008019
    CSI 2.06
    rCSI 5.23%
    PRS 84.75
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 1.97
    rCSI 2.45%
    PRS 74.52
  • radial glial cell CL0000681
    CSI 1.96
    rCSI 2.73%
    PRS 88.44
  • progenitor cell CL0011026
    CSI 1.83
    rCSI 3.89%
    PRS 83.99
  • enteroendocrine cell CL0000164
    CSI 1.83
    rCSI 2.5%
    PRS 88.45
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.76
    rCSI 2.95%
    PRS 76.68
  • glioblast CL0000030
    CSI 1.6
    rCSI 2.55%
    PRS 82.54
  • type B pancreatic cell CL0000169
    CSI 1.55
    rCSI 3.44%
    PRS 89.82
  • lung ciliated cell CL1000271
    CSI 1.55
    rCSI 1.79%
    PRS 84.25
  • club cell CL0000158
    CSI 1.49
    rCSI 2.18%
    PRS 85.27
  • pancreatic ductal cell CL0002079
    CSI 1.38
    rCSI 2.69%
    PRS 91.51
  • promonocyte CL0000559
    CSI 1.33
    rCSI 2.27%
    PRS 92.2
  • microcirculation associated smooth muscle cell CL0008035
    CSI 1.06
    rCSI 3.08%
    PRS 88.95
  • stromal cell CL0000499
    CSI 1
    rCSI 2.82%
    PRS 85.95
  • forebrain radial glial cell CL0013000
    CSI 0.93
    rCSI 2.98%
    PRS 89.74
  • primitive red blood cell CL0002355
    CSI 0.88
    rCSI 4.74%
    PRS 92
  • erythroid progenitor cell CL0000038
    CSI 0.59
    rCSI 3.38%
    PRS 92.01
  • Cajal-Retzius cell CL0000695
    CSI 0.54
    rCSI 4.25%
    PRS 91.72
  • effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062
    CSI 0.41
    rCSI 2.04%
    PRS 96.69

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [MOSPD3](/details-gene/64598), or Motile Sperm Domain Containing 3, is a protein-coding gene located on human chromosome 7q22.1, a region characterized in detail by large-scale sequencing efforts ([Link](https://doi.org/10.1101/gr.8.10.1060), [Link](https://doi.org/10.1038/nature01782)). While its name implies a role related to sperm motility, functional data suggests a broader involvement in fundamental cellular processes. The gene product is annotated as a [protein binding](/details-cell/GO:0005515) partner located in the [cytoplasm](/details-cell/GO:0005737) and at the [membrane](/details-cell/GO:0016020), with a potential role in [heart development](/details-cell/GO:0007507). Expression analysis reveals a wide distribution across various tissues, with its most significant expression signature observed in [interneuron](/details-cell/CL0000099)s, suggesting a specialized function in the nervous system. ## Cellular Roles and Expression Landscape The expression profile of [MOSPD3](/details-gene/64598) indicates a multifaceted role across diverse cell lineages. **Overall**, its most striking significance is within the nervous system, showing the highest Cell Significance Index (CSI) in [interneuron](/details-cell/CL0000099)s (CSI: 8.65), as well as notable significance in [neural progenitor cell](/details-cell/CL0011020)s and [neural crest cell](/details-cell/CL0011012)s. This pattern suggests a potential function in neuronal development, differentiation, or mature neuronal function. Beyond the nervous system, [MOSPD3](/details-gene/64598) is significantly expressed in cells of mesenchymal origin, including [mesodermal cell](/details-cell/CL0000222)s (CSI: 6.12) and [vascular associated smooth muscle cell](/details-cell/CL0000359)s (CSI: 4.01). This is consistent with its annotated role in development. Furthermore, the gene shows relevance in several hematopoietic and immune cell types, such as [intermediate monocyte](/details-cell/CL0002393)s (CSI: 5.48), [erythrocyte](/details-cell/CL0000232)s (CSI: 4.94), and [hematopoietic stem cell](/details-cell/CL0000037)s (CSI: 3.38). Significant expression is also noted in various epithelial cells, particularly those of the lung. This broad but distinct expression pattern suggests that [MOSPD3](/details-gene/64598) may be a housekeeping gene with specialized, context-dependent functions in different cell types. ## Pathways and Molecular Function Functional annotations provide clues to the molecular activities of [MOSPD3](/details-gene/64598). Its primary annotated molecular function is [protein binding](/details-cell/GO:0005515), indicating that it likely acts as an adapter, scaffold, or component of larger protein complexes. Its subcellular localization in the [cytoplasm](/details-cell/GO:0005737) and at the [membrane](/details-cell/GO:0016020) suggests it could mediate interactions between cytosolic signaling pathways and membrane-bound components. The most specific biological process associated with [MOSPD3](/details-gene/64598) is [heart development](/details-cell/GO:0007507). This annotation aligns well with its significant expression in [mesodermal cell](/details-cell/CL0000222)s and [vascular associated smooth muscle cell](/details-cell/CL0000359)s, both of which are critical lineages in cardiovascular organogenesis. It is plausible that [MOSPD3](/details-gene/64598) participates in protein-protein interaction networks that guide the differentiation or function of these cardiac and vascular cell types. ## Research Directions The diverse expression profile of [MOSPD3](/details-gene/64598), particularly the high significance in neurons juxtaposed with a developmental role in the heart, opens several avenues for future research. **Proposed Hypotheses:** 1. Given its high significance in [interneuron](/details-cell/CL0000099)s and its function as a [protein binding](/details-cell/GO:0005515) partner, [MOSPD3](/details-gene/64598) may function as a scaffolding protein at the neuronal membrane or cytoplasm, critical for organizing signaling complexes that regulate synaptic transmission or neuronal excitability. 2. Based on its annotated role in [heart development](/details-cell/GO:0007507) and expression in cardiac precursor cells, [MOSPD3](/details-gene/64598) may be essential for the specification or migration of cardiomyocytes or smooth muscle cells during embryogenesis. Its loss-of-function could potentially be linked to congenital heart defects. 3. The presence of [MOSPD3](/details-gene/64598) in hematopoietic lineages, including [hematopoietic stem cell](/details-cell/CL0000037)s and [monocyte](/details-cell/CL0002393)s, suggests it could play a role in the maintenance of stemness or the differentiation of myeloid cells. **Key Experimental Approach:** To test the hypothesis of [MOSPD3](/details-gene/64598)'s role in interneuron function (Hypothesis 1), a targeted approach is warranted. A conditional knockout of [MOSPD3](/details-gene/64598) in specific interneuron populations in mice, using a Cre-lox system (e.g., *Gad2*-Cre), would be highly informative. Subsequent analysis using patch-clamp electrophysiology on brain slices could directly assess changes in synaptic currents and neuronal firing properties, while co-immunoprecipitation followed by mass spectrometry from isolated interneurons could identify its binding partners and place it within specific signaling pathways. **Therapeutic Potential:** The broad expression of [MOSPD3](/details-gene/64598) across multiple, essential cell types (neural, hematopoietic, mesenchymal) suggests that it would be a challenging therapeutic target for systemic inhibition or activation due to a high risk of on-target, off-tissue toxicities. Any therapeutic strategy would likely require a highly targeted delivery system or focus on disease states where [MOSPD3](/details-gene/64598) expression becomes uniquely and pathogenically upregulated in a specific cell population.

Genular Protein ID: 399056407

Symbol: MSPD3_HUMAN

Name: Motile sperm domain-containing protein 3

UniProtKB Accession Codes:

O75425 A4D2D1 A6NG17 C9JE89 D6W5W1 O75423 O75424

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 2 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 11 Ensembl 4 ExpressionAtlas 1 GO 3 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 4 Pumba 1 RNAct 1 RefSeq 6 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9799793

Title: Large-scale sequencing of two regions in human chromosome 7q22: analysis of 650 kb of genomic sequence around the EPO and CUTL1 loci reveals 17 genes.

PubMed ID: 9799793

DOI: 10.1101/gr.8.10.1060

PubMed ID: 12853948

Title: The DNA sequence of human chromosome 7.

PubMed ID: 12853948

DOI: 10.1038/nature01782

PubMed ID: 12690205

Title: Human chromosome 7: DNA sequence and biology.

PubMed ID: 12690205

DOI: 10.1126/science.1083423

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 235
  • Mass: 25519
  • Checksum: 405D69B8309C4FAF
  • Sequence:
    • MRRGAPQDQE LVGPGPPGRG SRGAPPPLGP VVPVLVFPPD LVFRADQRSG PRQLLTLYNP 
TGTALRFRVL CTAPAKYTVF DAEGYVKPQS CIDIVIRHVA PIPSHYDVQD RFRIELSEEG 
AEGRVVGRKD ITSILRAPAY PLELQGQPDP APRPGPPAGT PPPTARHFQE HPRQQLATSS 
FLLFLLTGIV SVAFLLLPLP DELGSQLPQV LHVSLGQKLV AAYVLGLLTM VFLRT