Details for: GUSBP5

Gene ID: 441046

Gene Type:  Pseudogene  - A non-functional segment of DNA that resembles a functional gene but has lost its protein-coding ability or is otherwise no longer expressed.

Symbol: GUSBP5

Ensembl ID: ENSG00000251600

Description: GUSB pseudogene 5

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • VIP GABAergic cortical interneuron CL4023016
    CSI 22.71
    rCSI 27.12%
    PRS 95.53
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 12.3
    rCSI 29.41%
    PRS 94.37
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 11.67
    rCSI 14.51%
    PRS 94.9
  • cardiac muscle cell CL0000746
    CSI 11.32
    rCSI 16.24%
    PRS 95.67
  • melanocyte CL0000148
    CSI 11.11
    rCSI 8.23%
    PRS 97.86
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 10.47
    rCSI 17.57%
    PRS 95.82
  • sncg GABAergic cortical interneuron CL4023015
    CSI 9.79
    rCSI 15.75%
    PRS 95.45
  • pulmonary alveolar type 2 cell CL0002063
    CSI 9.22
    rCSI 14.3%
    PRS 98.57
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 8.76
    rCSI 28.79%
    PRS 93.85
  • Mueller cell CL0000636
    CSI 8.63
    rCSI 19.7%
    PRS 96.66
  • neuron CL0000540
    CSI 8.24
    rCSI 21.94%
    PRS 93.07
  • glycinergic amacrine cell CL4030028
    CSI 7.46
    rCSI 19.43%
    PRS 95.48
  • rod bipolar cell CL0000751
    CSI 7.13
    rCSI 12.81%
    PRS 96.88
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 6.66
    rCSI 16.2%
    PRS 94.34
  • GABAergic amacrine cell CL4030027
    CSI 6.65
    rCSI 22.77%
    PRS 93.22
  • renal interstitial pericyte CL1001318
    CSI 6.53
    rCSI 17.98%
    PRS 98.49
  • mesothelial cell CL0000077
    CSI 6.25
    rCSI 24.45%
    PRS 94.35
  • retinal ganglion cell CL0000740
    CSI 5.83
    rCSI 12.87%
    PRS 95.4
  • L6b glutamatergic cortical neuron CL4023038
    CSI 5.67
    rCSI 17.71%
    PRS 95.64
  • cerebral cortex neuron CL0010012
    CSI 5.57
    rCSI 22.7%
    PRS 95.23
  • glutamatergic neuron CL0000679
    CSI 5.56
    rCSI 11.43%
    PRS 93.32
  • sst GABAergic cortical interneuron CL4023017
    CSI 5.56
    rCSI 7.17%
    PRS 96.11
  • amacrine cell CL0000561
    CSI 5.56
    rCSI 16.1%
    PRS 95.73
  • endocardial cell CL0002350
    CSI 5.36
    rCSI 25.68%
    PRS 97.49
  • adipocyte CL0000136
    CSI 5.17
    rCSI 6.63%
    PRS 96.24
  • kidney connecting tubule epithelial cell CL1000768
    CSI 5.02
    rCSI 12.73%
    PRS 97.56
  • retinal cone cell CL0000573
    CSI 4.81
    rCSI 7.74%
    PRS 95.85
  • basal cell CL0000646
    CSI 4.46
    rCSI 5.97%
    PRS 97.78
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 4.26
    rCSI 15.31%
    PRS 94.72
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 4.17
    rCSI 7.36%
    PRS 95.57
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 4.14
    rCSI 7.51%
    PRS 96.19
  • parietal epithelial cell CL1000452
    CSI 3.86
    rCSI 10.33%
    PRS 97.59
  • GABAergic neuron CL0000617
    CSI 3.81
    rCSI 12.77%
    PRS 93.03
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 3.33
    rCSI 12.6%
    PRS 94.99
  • ON parasol ganglion cell CL4033052
    CSI 3.16
    rCSI 44.83%
    PRS 94.97
  • retinal pigment epithelial cell CL0002586
    CSI 3.12
    rCSI 6.19%
    PRS 97.29
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 2.87
    rCSI 16.89%
    PRS 95.16
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 2.67
    rCSI 6.9%
    PRS 98.28
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 2.17
    rCSI 6.79%
    PRS 96.09
  • ON midget ganglion cell CL4033046
    CSI 2.15
    rCSI 43.78%
    PRS 94.96
  • kidney distal convoluted tubule epithelial cell CL1000849
    CSI 2.07
    rCSI 21.94%
    PRS 97.1
  • direct pathway medium spiny neuron CL4023026
    CSI 1.77
    rCSI 42.3%
    PRS 93.6
  • indirect pathway medium spiny neuron CL4023029
    CSI 1.75
    rCSI 42.29%
    PRS 93.25
  • S cone cell CL0003050
    CSI 1.69
    rCSI 7.44%
    PRS 96.73
  • starburst amacrine cell CL0004232
    CSI 1.61
    rCSI 13.55%
    PRS 91.58
  • cardiac blood vessel endothelial cell CL0010006
    CSI 1.25
    rCSI 8.87%
    PRS 96.69
  • pulmonary alveolar type 1 cell CL0002062
    CSI 0.75
    rCSI 4.34%
    PRS 97.6

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [GUSBP5](/details-gene/441046) is a pseudogene located on chromosome 4q31.21, designated as GUSB pseudogene 5. Despite its classification as a pseudogene, which typically implies a lack of protein-coding potential, its expression profile suggests a potentially significant and highly specific role in the central nervous system. **Overall**, it demonstrates a high cell significance index (CSI) in diverse neuronal populations, including [VIP GABAergic cortical interneuron](/details-cell/CL4023016) (CSI: 22.71) and [L4 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4030063) (CSI: 12.30). Its notable expression in other distinct cell types such as [cardiac muscle cell](/details-cell/CL0000746) and [melanocyte](/details-cell/CL0000148) indicates a broader, though specific, expression landscape outside of the nervous system. ## Cellular Roles and Expression Landscape The expression pattern of [GUSBP5](/details-gene/441046) is strongly associated with the central nervous system, where it is a significant marker for a wide array of specialized neuronal subtypes. It shows the highest significance in cortical interneurons, including [VIP GABAergic cortical interneuron](/details-cell/CL4023016), [pvalb GABAergic cortical interneuron](/details-cell/CL4023018), [lamp5 GABAergic cortical interneuron](/details-cell/CL4023011), and [sncg GABAergic cortical interneuron](/details-cell/CL4023015). It is also highly significant in glutamatergic projection neurons, such as [L4 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4030063) and [L5/6 near-projecting glutamatergic neuron](/details-cell/CL4030067), suggesting a broad role across both inhibitory and excitatory circuits in the cortex. Beyond the cortex, [GUSBP5](/details-gene/441046) is a notable marker in retinal cells, including [Mueller cell](/details-cell/CL0000636), [glycinergic amacrine cell](/details-cell/CL4030028), and [rod bipolar cell](/details-cell/CL0000751). Outside the nervous system, its expression is significant in functionally diverse cells like [cardiac muscle cell](/details-cell/CL0000746), [melanocyte](/details-cell/CL0000148), and [pulmonary alveolar type 2 cell](/details-cell/CL0002063). This pattern suggests that its transcript may be involved in cellular processes common to these otherwise disparate, highly specialized cell types. The absence of data on cell types with low expression limits a definitive conclusion on its overall tissue specificity. ## Pathways and Molecular Function Functional annotation data for [GUSBP5](/details-gene/441046) are not provided. As a pseudogene of *GUSB* (beta-glucuronidase), it is presumed to be non-coding. The parent gene, *GUSB*, encodes a lysosomal hydrolase critical for the degradation of glycosaminoglycans. While [GUSBP5](/details-gene/441046) is unlikely to produce a functional enzyme, if transcribed, it could function as a non-coding RNA. Such transcripts can play regulatory roles, for example by acting as microRNA sponges or scaffolds for protein complexes, thereby influencing cellular pathways. Its high expression in metabolically active cells like neurons and cardiac muscle could suggest a potential role in modulating the expression or function of its parent gene, *GUSB*, or other related metabolic pathways. ## Research Directions The specific and high-level expression of a pseudogene like [GUSBP5](/details-gene/441046) in distinct neuronal and other specialized cell types warrants further investigation into its potential non-coding functions. **Proposed Hypotheses:** 1. **[GUSBP5](/details-gene/441046) functions as a competitive endogenous RNA (ceRNA) in cortical neurons.** Given its high expression in these cells, the [GUSBP5](/details-gene/441046) transcript may act as a microRNA "sponge," sequestering miRNAs that would otherwise target and repress key mRNAs involved in neuronal identity, synaptic function, or metabolic homeostasis. 2. **The [GUSBP5](/details-gene/441046) transcript plays a role in regulating chromatin structure or transcription in a cell-type-specific manner.** Some long non-coding RNAs act as guides or scaffolds for chromatin-modifying complexes. The expression of [GUSBP5](/details-gene/441046) could be necessary for establishing or maintaining the unique transcriptional programs of the cell types in which it is highly expressed, such as GABAergic interneurons or cardiac muscle cells. **Suggested Experimental Approach:** To test the hypothesis that [GUSBP5](/details-gene/441046) functions as a ceRNA in cortical neurons (Hypothesis 1), a multi-step approach could be employed. First, confirm the expression and localization of the [GUSBP5](/details-gene/441046) transcript in human iPSC-derived cortical interneurons using single-molecule fluorescence in situ hybridization (smFISH). Next, utilize antisense oligonucleotides (ASOs) to specifically knock down the [GUSBP5](/details-gene/441046) transcript. RNA-sequencing combined with bioinformatic analysis of miRNA binding sites on the [GUSBP5](/details-gene/441046) sequence would identify both the miRNAs it might sponge and the potential derepressed target mRNAs. Functional validation could be achieved by overexpressing candidate miRNAs and observing if they phenocopy the ASO-mediated knockdown effect, which could be assessed via electrophysiological recordings or morphological analysis. **Therapeutic Potential:** As a pseudogene, [GUSBP5](/details-gene/441046) is a non-traditional therapeutic target. However, if its non-coding RNA transcript is found to be a key regulator of neuronal function and is implicated in neurological or psychiatric disorders, it could represent a novel target for RNA-based therapeutics. Given its high specificity for certain neuronal subtypes, ASO-mediated inhibition of the [GUSBP5](/details-gene/441046) transcript could offer a precise way to modulate specific neural circuits with potentially fewer off-target effects than targeting widely expressed protein-coding genes. Its therapeutic relevance is entirely dependent on future research validating a functional, and potentially pathogenic, role.