Details for: HYDIN

Gene ID: 54768

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: HYDIN

Ensembl ID: ENSG00000157423

Description: HYDIN axonemal central pair apparatus protein

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • lung ciliated cell CL1000271
    CSI 28.57
    rCSI 33.04%
    PRS 94.92
  • ependymal cell CL0000065
    CSI 28.21
    rCSI 57.25%
    PRS 88.14
  • ciliated epithelial cell CL0000067
    CSI 26.97
    rCSI 23.71%
    PRS 93.36
  • multi-ciliated epithelial cell CL0005012
    CSI 21.01
    rCSI 20.96%
    PRS 94.76
  • ciliated cell CL0000064
    CSI 18.72
    rCSI 30.33%
    PRS 93.87
  • choroid plexus epithelial cell CL0000706
    CSI 15.47
    rCSI 25.33%
    PRS 94.77
  • retinal rod cell CL0000604
    CSI 12.26
    rCSI 21.61%
    PRS 95.06
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 12.12
    rCSI 17.18%
    PRS 96.79
  • microglial cell CL0000129
    CSI 10.15
    rCSI 40.84%
    PRS 95.75
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 9.25
    rCSI 11.51%
    PRS 91.1
  • L2/3 intratelencephalic projecting glutamatergic neuron CL4030059
    CSI 9.24
    rCSI 20.05%
    PRS 90.66
  • squamous epithelial cell CL0000076
    CSI 8.54
    rCSI 20.27%
    PRS 94.38
  • inhibitory interneuron CL0000498
    CSI 6.73
    rCSI 15.53%
    PRS 93.3
  • Mueller cell CL0000636
    CSI 6.57
    rCSI 15%
    PRS 94.68
  • kidney connecting tubule epithelial cell CL1000768
    CSI 6.54
    rCSI 16.59%
    PRS 95.27
  • glioblast CL0000030
    CSI 6.32
    rCSI 10.09%
    PRS 94.02
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 6.1
    rCSI 21.95%
    PRS 91.17
  • S cone cell CL0003050
    CSI 6.1
    rCSI 26.8%
    PRS 94.7
  • sst GABAergic cortical interneuron CL4023017
    CSI 5.92
    rCSI 7.63%
    PRS 92.97
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 5.46
    rCSI 9.17%
    PRS 92.64
  • hepatic stellate cell CL0000632
    CSI 5.34
    rCSI 20.02%
    PRS 96.33
  • glutamatergic neuron CL0000679
    CSI 5.2
    rCSI 10.68%
    PRS 90.6
  • mesothelial cell CL0000077
    CSI 5.19
    rCSI 20.29%
    PRS 90.44
  • secretory cell CL0000151
    CSI 4.98
    rCSI 5.2%
    PRS 96.98
  • retinal bipolar neuron CL0000748
    CSI 4.72
    rCSI 8.84%
    PRS 93.54
  • epithelial cell of proximal tubule CL0002306
    CSI 4.58
    rCSI 11.19%
    PRS 94.03
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 4.47
    rCSI 10.18%
    PRS 93.33
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 4.19
    rCSI 13.77%
    PRS 91.05
  • regular ventricular cardiac myocyte CL0002131
    CSI 3.95
    rCSI 24.66%
    PRS 93.98
  • parietal epithelial cell CL1000452
    CSI 3.8
    rCSI 10.15%
    PRS 95.71
  • sncg GABAergic cortical interneuron CL4023015
    CSI 3.68
    rCSI 5.93%
    PRS 92.68
  • retinal cone cell CL0000573
    CSI 3.61
    rCSI 5.81%
    PRS 93.45
  • chondrocyte CL0000138
    CSI 3.32
    rCSI 5.28%
    PRS 95.57
  • VIP GABAergic cortical interneuron CL4023016
    CSI 3.23
    rCSI 3.86%
    PRS 92.42
  • deuterosomal cell CL4033044
    CSI 3.17
    rCSI 10.71%
    PRS 93.74
  • GABAergic neuron CL0000617
    CSI 2.96
    rCSI 9.9%
    PRS 90.08
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 2.95
    rCSI 7.05%
    PRS 91.94
  • cerebral cortex neuron CL0010012
    CSI 2.89
    rCSI 11.76%
    PRS 92.93
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 2.7
    rCSI 6.56%
    PRS 91.02
  • L6b glutamatergic cortical neuron CL4023038
    CSI 2.68
    rCSI 8.38%
    PRS 92.93
  • astrocyte of the cerebral cortex CL0002605
    CSI 2.57
    rCSI 5.75%
    PRS 92.78
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 2.51
    rCSI 6.49%
    PRS 96.6
  • cardiac muscle cell CL0000746
    CSI 2.48
    rCSI 3.55%
    PRS 93.2
  • retinal ganglion cell CL0000740
    CSI 2.35
    rCSI 5.19%
    PRS 92.48
  • macroglial cell CL0000126
    CSI 2.13
    rCSI 5.49%
    PRS 95.4
  • renal principal cell CL0005009
    CSI 2.1
    rCSI 5.46%
    PRS 97.15
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 2.06
    rCSI 7.79%
    PRS 92.19
  • direct pathway medium spiny neuron CL4023026
    CSI 1.98
    rCSI 47.36%
    PRS 90.22
  • indirect pathway medium spiny neuron CL4023029
    CSI 1.96
    rCSI 47.2%
    PRS 90
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.66
    rCSI 2.93%
    PRS 92.39
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 1.3
    rCSI 7.63%
    PRS 92.45
  • glial cell CL0000125
    CSI 1.24
    rCSI 4.72%
    PRS 94.17
  • central nervous system neuron CL2000029
    CSI 0.99
    rCSI 7.28%
    PRS 93.76
  • medium spiny neuron CL1001474
    CSI 0.38
    rCSI 3.23%
    PRS 93.35

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [HYDIN](/details-gene/54768) encodes the HYDIN axonemal central pair apparatus protein, a critical component of motile cilia. The gene's primary function is associated with the assembly and regulation of the axonemal central apparatus, which is essential for orchestrating the complex beating patterns of cilia and flagella. Consistent with this role, expression of [HYDIN](/details-gene/54768) is overwhelmingly concentrated in cell types characterized by the presence of motile cilia, such as [lung ciliated cells](/details-cell/CL1000271) and [ependymal cells](/details-cell/CL0000065). Mutations in this gene are known to cause Primary Ciliary Dyskinesia (PCD), a genetic disorder characterized by impaired mucociliary clearance and chronic respiratory infections ([Link](https://doi.org/10.1016/j.ajhg.2012.08.016)). ## Cellular Roles and Expression Landscape The expression profile of [HYDIN](/details-gene/54768) underscores its specialized function in ciliary motility. **Overall**, the gene shows exceptionally high significance in a narrow range of ciliated cell types. It is most prominent in the [lung ciliated cell](/details-cell/CL1000271) (CSI: 28.57), [ependymal cell](/details-cell/CL0000065) (CSI: 28.21), and [ciliated epithelial cell](/details-cell/CL0000067) (CSI: 26.97), which line the respiratory tract and the ventricles of the brain, respectively. Its high expression in these cells is critical for mucociliary clearance in the airways and cerebrospinal fluid circulation in the brain. The gene is also significantly expressed in [choroid plexus epithelial cells](/details-cell/CL0000706), which are involved in producing cerebrospinal fluid, and in [retinal rod cells](/details-cell/CL0000604), where the connecting cilium plays a vital role in photoreceptor function. The data also suggests moderate expression in certain neuronal populations, including [pvalb GABAergic cortical interneurons](/details-cell/CL4023018) and [L2/3 intratelencephalic projecting glutamatergic neurons](/details-cell/CL4030059), which may point to a role in the function of primary cilia in the central nervous system. ## Pathways and Molecular Function The functional annotations for [HYDIN](/details-gene/54768) are tightly correlated with its observed cellular expression pattern. The gene is a key participant in biological processes such as `Axonemal central apparatus assembly` ([GO:1904158](https://www.ebi.ac.uk/QuickGO/term/GO:1904158)) and `Cilium movement` ([GO:0003341](https://www.ebi.ac.uk/QuickGO/term/GO:0003341)). These functions are executed within the `Axonemal central apparatus` ([GO:1990716](https://www.ebi.ac.uk/QuickGO/term/GO:1990716)) and the larger `Axoneme` ([GO:0005930](https://www.ebi.ac.uk/QuickGO/term/GO:0005930)) structure. Research has confirmed that [HYDIN](/details-gene/54768) is essential for maintaining the stability of the central pair apparatus, and its absence leads to disorganized ciliary beating patterns ([Link](https://doi.org/10.1083/jcb.200701113), [Link](https://doi.org/10.1183/09031936.00052014)). The gene's involvement in developmental processes like `Trachea development` ([GO:0060438](https://www.ebi.ac.uk/QuickGO/term/GO:0060438)) and `Ventricular system development` ([GO:0021591](https://www.ebi.ac.uk/QuickGO/term/GO:0021591)) further highlights its fundamental role in establishing tissues dependent on ciliary function. ## Research Directions Given that recessive mutations in [HYDIN](/details-gene/54768) are a known cause of Primary Ciliary Dyskinesia (PCD), research is primarily focused on understanding the precise mechanisms of the disease and developing potential therapies. The expression data in less-characterized cell types suggests new avenues of investigation. **Testable Hypotheses:** 1. The significant expression of [HYDIN](/details-gene/54768) in [retinal rod cells](/details-cell/CL0000604) suggests that some loss-of-function variants, perhaps those that are hypomorphic rather than null, could contribute to subtle retinal ciliopathies or vision defects that may be overlooked in classic PCD diagnoses. 2. The presence of [HYDIN](/details-gene/54768) transcripts in neuronal subtypes like [inhibitory interneurons](/details-cell/CL0000498) implies a potential role in the structure or signaling function of primary cilia in the brain. Disruption of [HYDIN](/details-gene/54768) in these neurons could contribute to neurological or neurodevelopmental phenotypes that may co-occur with PCD. **Proposed Experimental Approach:** To test the first hypothesis regarding the role of [HYDIN](/details-gene/54768) in retinal function, a conditional knockout mouse model could be generated where [HYDIN](/details-gene/54768) is specifically deleted in photoreceptor cells. The phenotype would be assessed using electroretinography (ERG) to measure retinal electrical responses to light stimuli. Furthermore, transmission electron microscopy (TEM) could be used to examine the ultrastructure of the connecting cilium in photoreceptors to determine if its morphology is compromised in the absence of [HYDIN](/details-gene/54768). **Therapeutic Potential:** As PCD caused by [HYDIN](/details-gene/54768) mutations is a loss-of-function, monogenic disorder, the gene represents a prime candidate for **gene replacement therapy**. The therapeutic strategy would involve delivering a functional copy of the [HYDIN](/details-gene/54768) cDNA to the affected ciliated epithelial cells, particularly in the respiratory tract, to restore normal ciliary beat and mucociliary clearance. Challenges would include the large size of the gene and achieving efficient, widespread, and sustained delivery to the target cells, likely via an adeno-associated virus (AAV) or lentiviral vector administered through inhalation. This approach represents a potential curative strategy rather than one of inhibition.

Genular Protein ID: 3960717985

Symbol: HYDIN_HUMAN

Name: Hydrocephalus-inducing protein homolog

UniProtKB Accession Codes:

Q4G0P3 A6NC70 A6NLZ0 B4DQY4 B4DRN4 F5H6V3 Q8N3H8 Q8N3P6 Q8TC08 Q96JG3 Q96SS4 Q9H5U3 Q9H9B8 Q9NTI0 Q9UBE5

Database IDs:

AGR 1 Antibodypedia 1 BMRB 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 4 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 12 Ensembl 4 EvolutionaryTrace 1 ExpressionAtlas 1 GO 8 Gene3D 2 GeneCards 1 GeneReviews 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 3 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PDB 2 PDBsum 2 PIR 2 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 6 RNAct 1 RefSeq 4 SIGNOR 1 SMR 1 STRING 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15616553

Title: The sequence and analysis of duplication-rich human chromosome 16.

PubMed ID: 15616553

DOI: 10.1038/nature03187

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 16938426

Title: A 360-kb interchromosomal duplication of the human HYDIN locus.

PubMed ID: 16938426

DOI: 10.1016/j.ygeno.2006.07.012

PubMed ID: 17296793

Title: Hydin seek: finding a function in ciliary motility.

PubMed ID: 17296793

DOI: 10.1083/jcb.200701113

PubMed ID: 23022101

Title: Recessive HYDIN mutations cause primary ciliary dyskinesia without randomization of left-right body asymmetry.

PubMed ID: 23022101

DOI: 10.1016/j.ajhg.2012.08.016

PubMed ID: 25186273

Title: Ciliary beat pattern and frequency in genetic variants of primary ciliary dyskinesia.

PubMed ID: 25186273

DOI: 10.1183/09031936.00052014

Sequence Information:

  • Length: 5121
  • Mass: 575892
  • Checksum: 47FB2A11C7E50A4F
  • Sequence:
    • MTSRRLEESM GAVQMGLVNM FKGFQSKVLP PLSPKVVTEE EVNRMLTPSE FLKEMSLTTE 
QRLAKTRLMC RPQIIELLDM GETTHQKFSG IDLDQALFQP FPSEIIFQNY TPCEVYEVPL 
ILRNNDKIPR LVKVVEESSP YFKVISPKDI GHKVAPGVPS IFRILFTPEE NKDYAHTLTC 
VTEREKFIVP IKARGARAIL DFPDKLNFST CPVKYSTQKI LLVRNIGNKN AVFHIKTCRP 
FSIEPAIGTL NVGESMQLEV EFEPQSVGDH SGRLIVCYDT GEKVFVSLYG AAIDMNIRLD 
KNSLTIEKTY ISLANQRTIT IHNRSNIIAH FLWKVFATQQ EEDREKYRAC DDLIKEEKDE 
TDEFFEECIT DPLLREHLSV LSRTFANQRR LVQGDSKLFF NNVFTVEPLE GDVWPNSSAE 
ITVYFNPLEA KLYQQTIYCD ILGREIRLPL RIKGEGMGPK IHFNFELLDI GKVFTGSAHC 
YEAILYNKGS IDALFNMTPP TSALGACFVF SPKEGIIEPS GVQAIQISFS STILGNFEEE 
FLVNVNGSPE PVKLTIRGCV IGPTFHFNVP ALHFGDVSFG FPHTLICSLN NTSLIPMTYK 
LRIPGDGLGH KSISYCEQHV DYKRPSWTKE EISSMKPKEF TISPDCGTIR PQGFAAIRVT 
LCSNTVQKYE LALVVDVEGI GEEVLALLIT ARCVVPALHL VNTEVDFGHC FLKYPYEKTL 
QLANQDDLPG FYEVQPQVCE EVPTVLFSSP TPSGVISPSS TIHIPLVLET QVTGEHRSTV 
YISIFGSQDP PLVCHLKSAG EGPVIYVHPN QVDFGNIYVL KDSSRILNLC NQSFIPAFFQ 
AHMAHKKSLW TIEPNEGMVP PETDVQLALT ANLNDTLTFK DCVILDIENS STYRIPVQAS 
GTGSTIVSDK PFAPELNLGA HFSLDTHYYH FKLINKGRRI QQLFWMNDSF RPQAKLSKKG 
RVKKGHAHVQ PQPSGSQEPR DPQSPVFHLH PASMELYPGQ AIDVILEGYS ATPRIVKEKL 
VCHAIIGAQK GKSLVMAVNI TCEFVAPLIQ LSTKQLIYRL EKKPNSILKP DYQPLAIKNI 
STLPVNLLLS TSGPFFICET DKSLLPATPE PIKLEIDEEK NLLIKFDPSY RNDLNNWVAE 
EILAIKYVEH PQIDSLDLRG EVHYPNLSFE TKELDFGCIL NDTELIRYVT ITNCSPLVVK 
FRWFFLVNDE ENQIRFVTLP KKPYSAPVSQ MESIPATSEA ASPPAILVTV ESPEMDLNDF 
VKTVLVDEDA RPEEKELRKT KASSVISDEI KISSTEIERI YSSQSQVEDQ ESLQTCEQNE 
MLSIGIEEVF DILPLFGVLQ PHSSHQISFT FYGHANIIAQ AKALCEVEEG PTYEITLKGE 
ASLVNYSFDT KDIHYGLQLF DHVTEREITL TNMGKVGFEF KVLTDHQSSP DNLLPGVPLI 
LPVSGFISSH QEQVLKVYYL PGVPEVFKRS FQIQIAHLDP ENITLSGEGI FPQICLDLPR 
NLTANEKYEM FLNQARKNTD KEYNKCEMLD HFDIITEEVP EDEPAEVSAH LQMEVERLIV 
QSYVLEHQKT TTPDPMDDPC FSHRSRRKLA KIQLPEYILD FGYIILGEVR THIIKIINTS 
HFPVSFHADK RVLHETGFST ELDRVKNLPH CETEIFEVRF DPQGANLPVG SKEVILPIKV 
VGGPTVHICL QAKVTIPTMT LSRGKVDFAT IQCGQCLVET IQLSNHLQVP CEWFVQSQKP 
VDKLEKHMPK YLRQKLRAEL KPKTRIFEIQ PISGVLDPGE KSNVQVKFMP KEEKFYSQTL 
VFQIAQSAQK LTLLARGQGL EPRLEFSPSV LDLGPLLLCA PGDEAEVIVK NPCNFPIEFY 
SLEFDQQYLI EEKILRKLKG YDSYNTLLLP PRNPGEKLPP ELYEYFKEIK KSKEEQMRAK 
YLENLAQENE EEDITSSDQG TSNSTKRTSL SRGISVTSNL EEWHALLVES KTYLEEEEDE 
ESLEKIIFQT DKLQSIDSHS MEEVGEVENN PVSKAIARHL GIDISAEGRL AKNRKGIAII 
IHGTPLSGKS ANAVSVAKYY NAACLSIDSI VLEAVANSNN IPGIRARELC IRAAIEQSVK 
EGEEAAQEAA VGQNVIGQGR LSTDTLGKLA SEMTLVAPEI KPGKSVRGSV VITKSKADSH 
GSGSQKQHHS HQSETPQISS SPLPPGPIHR WLSVSPSVGG ETGLMSCVLP DELLVQILAE 
RIQLSDCYRG VVFDGLDTLF AQNAAAALLC LLKAIGSREH IYILNMAQDY AAMKAQEKAK 
KEQEERKHKG ALEKEKERLQ NMDEEEYDAL TEEEKLTFDR GIQQALRERK KREQERLAKE 
MQEKKLQQEL ERQKEEDELK RRVKKGKQGP IKEEPPMKKS QAANKQVPPL TKVDVKMETI 
ERKISVREQT MSEKEELNKK KRNMGDVSMH GLPLVQDQED SEGDNSKDPD KQLAPKFKTY 
ELTLKDVQNI LMYWDRKQGV QLPPAGMEEA PHEPDDQRQV PLGGRRGRKD RERERLEKER 
TEKERLEREK AERERLEKLR ALEERSDWEG EGEEDHEGKK EKDLGVPFLD IQTPDFEGLS 
WKQALESDKL PKGEQILDIL GLGASGPPIP PPALFSIVSY PVKRPPLTMT DDLEHFVFVI 
PPSEDISLDE KKEMEIESDF LATTNTTKAQ EEQTSSSKGG KQKMKEKIDQ VFEIQKDKRH 
MALNRKVLSG EPAGTISQLS DTDLDNFNGQ HSQEKFTRLN HFRWIVPANG EVTLQVHFSS 
DEFGNFDQTF NFEILGTCCQ YQLYCRGICT YPYICQDPKV VFPQRKMDMK TNEVIFKKYV 
MSTETYYFGP LLCGKSRDKY KSSLFPGNME TLTILNTSLM VVEASFYFQN DVKANTYFLE 
PNTMVLKPNE KQILNVWAYP TSVGVFEDSI VCCINDNPEP AIFQLSCQGI RPELELEPRQ 
LHFDRLLLHR QESRVVLLRN VTLLPVAWRI TSLEHLGDDF TVSLMQGTIP PEAEYGLHLY 
FQPTKPVNIK KAIRLEVLDA ENLLGVVQIE NIMVFAEAYD IALDITFPKG AEGGLDFGIV 
RVTEEAKQPL QLKNRGKYEI AFSFSVDSVG ISTPNINSMI SVQPKKGSLT PTEKPTNVQV 
FFHAKKEVKI EHQPVLRCQI IEPNISEGGE IIASIPIKFS ANAVYSKYNI TPSSVINFGA 
LICGTRKSTT FTIENQGVTD FKFALYKLTG ESPIHQKKAA SHVRHARSRE SESFYKTGSS 
RAAKFSDTIQ KEVTTTGQAR FAHGMFTVYP GFGSIPSGGQ QVINVDCVAD AMGKCEEFIA 
IDISGRDPAV HPAGILYTLL AEACLPAFVT ENNALIFEEH QICTSANLHH ILQTIESGGL 
FVEDENKFIF CNVLVGRQAK ARFKISNVGK ITCDVNIVVR PISNKPFARI VDIFEVEPSK 
MCIASHSHAF ATVSFTPQIM QNYQCIFEAT LDGLPSTLAK SRGLVFDIAG EGNLPRVTVV 
RPVLHNQYGN PLLLFKRLLL GHSEKLPLIL KNNGVLPAQL HVDLQDELGV FSLKGRPTTA 
YIYITEENKP HVKAKKAHTA SLVVSPGDTA EFDVVFHSQK VGRMRGIIHL SVINNQYEET 
SIHMVGEGYE DDITLDNIHG LVAPTSQEDI SISEFTEIIE DNDMEDLVAA ALVDHIQFGD 
CHIGHSYNAS FTVTNHSQVN LIRFEWPVSA TIAFSPQMGH LHPGCAKDIV VTMKSDVPIN 
LKNMRIRCKL SRIMFQLPAD QVPDWDDRMH TVKWVDVPRN MPGTFTTKRK VIETDPEPAH 
SVLEENYQEL QLQISANVDF ASYHCQARDV RFKETLVYQT RVFEFDVINS GRVQLEFSWV 
SEDTSKAVSF AKPDHQGSAQ KDQLSQGTMH TGSTLDSTMD HWAEGSPQPF SVEPSSGIVP 
VGKIQKFKVK FSPLDIGDFE SNLFCQIPNL PPGEQGPVLV AKGRSTLPIC HFDLKDSDYI 
SGHQRNPELR GSSGGALDPN TRVIEFTTVG IGGKNLRTFT ILNPTNSTYS FCWISEEIES 
LQNPAAFTCL TEKGFIHPEK KAEIVFQFTP FHLGITESSW TFLIPEHNIT VPFLLVGKTT 
EPLISLNKSH LNFSSLLIGR EARETVQIIN KEEQGFDFSF QDNSRYSEGF SNSLLVCPME 
GWIPPLSRFP IDIFFTPKQE GDVNFNLICN VEKKVHPVTL NVKAEGYTMN VEIKCKDRTG 
SITLLTPNQT NIINFYEVEL NECVQCEFNF INTGKFTFSF QAQLCGSKTL LQYLEFSPID 
STVDVGQSVH ATLSFQPLKK CVLTDLELII KISHGPTFMC NISGCAVSPA IHFSFTSYNF 
GTCFIYQAGM PPYKQTLVIT NKEETPMSID CLYTNTTHLE VNSRVDVVKP GNTLEIPITF 
YPRESINYQE LIPFEINGLS QQTVEIKGKG TKMKILVLDP ANRIVKLGAV LPGQVVKRTV 
SIMNNSLAQL TFNQSILFTI PELQEPKVLT LAPFHNITLK PKEVCKLEVI FAPKKRVPPF 
SEEVFMECMG LLRPLFLLSG CCQALEISLD QEHIPFGPVV YQTQATRRIL MMNTGDVGAR 
FKWDIKKFEP HFSISPEEGY ITSGMEVSFE VTYHPTEVGK ESLCKNILCY IQGGSPLSLT 
LSGVCVGPPA VKEVVNFTCQ VRSKHTQTIL LSNRTNQTWN LHPIFEGEHW EGPEFITLEA 
HQQNKPYEIT YRPRTMNLEN RKHQGTLFFP LPDGTGWLYA LHGTSELPKA VANIYREVPC 
KTPYTELLPI TNWLNKPQRF RVIVEILKPE KPDLSITMKG LDYIDVLSGS KKDYKLNFFS 
HKEGTYAAKV IFRNEVTNEF LYYNVSFRVI PSGIIKTIEM VTPVRQVASA SIKLENPLPY 
SVTFSTECRM PDIALPSQFV VPANSEGTFS FEFQPLKAGE TFGRLTLHNT DLGYYQYELY 
LKATPALPEK PVHFQTVLGS SQIILVKFIN YTRQRTEYYC RTDCTDFHAE KLINAAPGGQ 
GGTEASVEVL FEPSHLGETK GILILSSLAG GEYIIPLFGM ALPPKPQGPF SIRAGYSIII 
PFKNVFYHMV TFSIIVDNPA FTIRAGESVR PKKINNITVS FEGNPSGSKT PITTKLTVSC 
PPGEGSETGV KWVYYLKGIT L