Details for: ZNF524

Gene ID: 147807

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF524

Ensembl ID: ENSG00000171443

Description: zinc finger protein 524

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • intermediate monocyte CL0002393
    CSI 9.78
    rCSI 14.76%
    PRS 91.87
  • central memory CD4-positive, alpha-beta T cell CL0000904
    CSI 7.7
    rCSI 4.55%
    PRS 97.55
  • vascular associated smooth muscle cell CL0000359
    CSI 7.27
    rCSI 23.57%
    PRS 85.64
  • neural progenitor cell CL0011020
    CSI 7.09
    rCSI 31.17%
    PRS 76.37
  • naive T cell CL0000898
    CSI 5.91
    rCSI 4.11%
    PRS 96.64
  • intestinal tuft cell CL0019032
    CSI 4.61
    rCSI 7.04%
    PRS 89.93
  • mature alpha-beta T cell CL0000791
    CSI 4.5
    rCSI 16.29%
    PRS 97.47
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 4.23
    rCSI 3.22%
    PRS 96.28
  • CD4-positive, alpha-beta memory T cell CL0000897
    CSI 3.45
    rCSI 2.48%
    PRS 96.34
  • secretory cell CL0000151
    CSI 3.2
    rCSI 3.34%
    PRS 86.49
  • epithelial cell of lower respiratory tract CL0002632
    CSI 3.14
    rCSI 2.43%
    PRS 90.61
  • skin fibroblast CL0002620
    CSI 3.1
    rCSI 2.67%
    PRS 87.25
  • group 3 innate lymphoid cell CL0001071
    CSI 3.05
    rCSI 2.3%
    PRS 91.49
  • common myeloid progenitor CL0000049
    CSI 3
    rCSI 2.43%
    PRS 89.31
  • pancreatic acinar cell CL0002064
    CSI 2.98
    rCSI 3.96%
    PRS 91.2
  • club cell CL0000158
    CSI 2.95
    rCSI 4.32%
    PRS 82.88
  • T follicular helper cell CL0002038
    CSI 2.92
    rCSI 2.19%
    PRS 95.81
  • acinar cell CL0000622
    CSI 2.85
    rCSI 4.18%
    PRS 93.89
  • myeloid leukocyte CL0000766
    CSI 2.77
    rCSI 2.56%
    PRS 88.67
  • mucus secreting cell CL0000319
    CSI 2.66
    rCSI 4.22%
    PRS 92.95
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 2.63
    rCSI 2.68%
    PRS 93.69
  • early lymphoid progenitor CL0000936
    CSI 2.62
    rCSI 2.3%
    PRS 91.31
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 2.55
    rCSI 2.5%
    PRS 96.32
  • mature B cell CL0000785
    CSI 2.5
    rCSI 2.18%
    PRS 94.3
  • perivascular cell CL4033054
    CSI 2.5
    rCSI 3.42%
    PRS 90.99
  • mucous neck cell CL0000651
    CSI 2.49
    rCSI 3.58%
    PRS 91.31
  • plasmablast CL0000980
    CSI 2.47
    rCSI 1.94%
    PRS 90.64
  • keratinocyte CL0000312
    CSI 2.44
    rCSI 2.05%
    PRS 88.92
  • epithelial cell of lung CL0000082
    CSI 2.43
    rCSI 2.01%
    PRS 88.67
  • intestine goblet cell CL0019031
    CSI 2.4
    rCSI 2.13%
    PRS 84.96
  • non-classical monocyte CL0000875
    CSI 2.4
    rCSI 3.85%
    PRS 93.29
  • transit amplifying cell of colon CL0009011
    CSI 2.4
    rCSI 2.82%
    PRS 88.34
  • goblet cell CL0000160
    CSI 2.39
    rCSI 2.25%
    PRS 85.61
  • elicited macrophage CL0000861
    CSI 2.32
    rCSI 2.13%
    PRS 92.83
  • foveolar cell of stomach CL0002179
    CSI 2.3
    rCSI 4.9%
    PRS 90.67
  • granulocyte monocyte progenitor cell CL0000557
    CSI 2.29
    rCSI 1.98%
    PRS 90.29
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 2.24
    rCSI 1.72%
    PRS 90.74
  • CD4-positive, alpha-beta cytotoxic T cell CL0000934
    CSI 2.24
    rCSI 3.07%
    PRS 96.87
  • stem cell CL0000034
    CSI 2.2
    rCSI 2.12%
    PRS 82.71
  • class switched memory B cell CL0000972
    CSI 2.13
    rCSI 1.59%
    PRS 95.34
  • multi-ciliated epithelial cell CL0005012
    CSI 2.1
    rCSI 2.1%
    PRS 81.83
  • duct epithelial cell CL0000068
    CSI 2.09
    rCSI 3.05%
    PRS 91.46
  • basal cell CL0000646
    CSI 2.03
    rCSI 2.71%
    PRS 85.11
  • colon epithelial cell CL0011108
    CSI 2.01
    rCSI 2.11%
    PRS 85.11
  • dendritic cell, human CL0001056
    CSI 1.99
    rCSI 3.05%
    PRS 93.73
  • intestinal epithelial cell CL0002563
    CSI 1.97
    rCSI 2.06%
    PRS 85.17
  • ciliated epithelial cell CL0000067
    CSI 1.96
    rCSI 1.72%
    PRS 78.34
  • BEST4+ enteroycte CL4030026
    CSI 1.93
    rCSI 2.39%
    PRS 87.5
  • promyelocyte CL0000836
    CSI 1.89
    rCSI 2.73%
    PRS 90.83
  • erythrocyte CL0000232
    CSI 1.82
    rCSI 4.13%
    PRS 86.73
  • enteroendocrine cell CL0000164
    CSI 1.68
    rCSI 2.3%
    PRS 86.44
  • peripheral nervous system neuron CL2000032
    CSI 1.6
    rCSI 2.18%
    PRS 80.74
  • Langerhans cell CL0000453
    CSI 1.58
    rCSI 2.42%
    PRS 94.89
  • pulmonary artery endothelial cell CL1001568
    CSI 1.5
    rCSI 2.04%
    PRS 92.84
  • lung ciliated cell CL1000271
    CSI 1.48
    rCSI 1.71%
    PRS 81.32
  • CD1c-positive myeloid dendritic cell CL0002399
    CSI 1.21
    rCSI 1.46%
    PRS 93.08
  • promonocyte CL0000559
    CSI 1.21
    rCSI 2.07%
    PRS 90.83
  • lung macrophage CL1001603
    CSI 1.17
    rCSI 2.61%
    PRS 92.6
  • colon goblet cell CL0009039
    CSI 1.17
    rCSI 2.77%
    PRS 90.34
  • tracheobronchial serous cell CL0019001
    CSI 0.82
    rCSI 3.56%
    PRS 91.17
  • microcirculation associated smooth muscle cell CL0008035
    CSI 0.68
    rCSI 1.98%
    PRS 86.65

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF524](/details-gene/147807), or Zinc Finger Protein 524, is a protein-coding gene located on chromosome 19q13.42. As its name suggests, it is predicted to function as a DNA-binding transcription factor, primarily operating within the [nucleus](/details-gene/GO:0005634) to regulate transcription ([GO:0006357](/details-gene/GO:0006357)). Expression data highlights its significance in a diverse array of cell types, suggesting a broad regulatory role. It is a particularly important gene in immune cells, with a very high Cell Significance Index (CSI) in [intermediate monocytes](/details-cell/CL0002393) and various T lymphocyte subsets, including [central memory CD4-positive, alpha-beta T cells](/details-cell/CL0000904). Its notable expression in non-hematopoietic cells such as [vascular associated smooth muscle cells](/details-cell/CL0000359) and [neural progenitor cells](/details-cell/CL0011020) indicates that its function extends beyond the immune system into areas of tissue development and maintenance. The gene was characterized as part of the Mammalian Gene Collection project ([Link](https://doi.org/10.1101/gr.2596504)). ## Cellular Roles and Expression Landscape The expression profile of [ZNF524](/details-gene/147807) reveals its involvement across multiple biological systems, with a prominent role in the immune and mesenchymal compartments. **Overall**, the gene's highest significance is observed in the myeloid lineage, specifically in [intermediate monocytes](/details-cell/CL0002393) (CSI: 9.78), a cell type associated with inflammatory responses. This suggests [ZNF524](/details-gene/147807) may be a key transcriptional regulator governing the function or differentiation state of these cells. Its role in the adaptive immune system is also strongly supported by high significance scores across various T cell populations. These include [central memory CD4-positive, alpha-beta T cells](/details-cell/CL0000904), [naive T cells](/details-cell/CL0000898), [mature alpha-beta T cells](/details-cell/CL0000791), and [effector CD8-positive, alpha-beta T cells](/details-cell/CL0001050). This pattern implies a fundamental role in T cell biology, potentially spanning from the maintenance of quiescence in naive cells to the execution of effector functions in mature cells. Furthermore, its expression in [group 3 innate lymphoid cells](/details-cell/CL0001071) and [common myeloid progenitors](/details-cell/CL0000049) suggests an early and broad role in hematopoietic development. Beyond the immune system, [ZNF524](/details-gene/147807) demonstrates significant expression in structural and progenitor cells. Its high CSI in [vascular associated smooth muscle cells](/details-cell/CL0000359) and [skin fibroblasts](/details-cell/CL0002620) is consistent with a function in regulating the phenotype or contractility of mesenchymal tissues. The gene's importance in [neural progenitor cells](/details-cell/CL0011020) points to a potential involvement in nervous system development or maintenance of progenitor cell identity. The broad but distinct expression pattern across these disparate cell types indicates that [ZNF524](/details-gene/147807) likely acts as a context-dependent transcriptional regulator controlling fundamental cellular programs. ## Pathways and Molecular Function Functional annotations corroborate the role of [ZNF524](/details-gene/147807) as a nuclear protein involved in gene expression regulation. Its molecular functions are dominated by activities related to DNA binding and transcriptional control. It is annotated with [Dna-binding transcription factor activity](/details-gene/GO:0003700), specifically binding to cis-regulatory regions of genes to control RNA polymerase II ([GO:0000978](/details-gene/GO:0000978)). This core function is supported by its localization to the [nucleus](/details-gene/GO:0005634) and its involvement in the broader biological process of [Regulation of transcription by rna polymerase ii](/details-gene/GO:0006357). The annotations for [Metal ion binding](/details-gene/GO:0046872) are consistent with its identity as a zinc finger protein, where zinc ions are crucial for stabilizing the DNA-binding domain. The connection of these molecular functions to its cellular expression profile suggests that [ZNF524](/details-gene/147807) likely directs specific gene expression programs that define the identity and function of monocytes, T cells, and smooth muscle cells. ## Research Directions The broad yet significant expression pattern of [ZNF524](/details-gene/147807) in key cell types of immunological and vascular relevance presents several avenues for future research. ### Proposed Hypotheses 1. **[ZNF524](/details-gene/147807) is a critical regulator of monocyte-to-macrophage differentiation and inflammatory activation.** Its peak expression in [intermediate monocytes](/details-cell/CL0002393), a transitional cell state, suggests it may control the transcriptional programs that mediate the maturation of circulating monocytes into tissue-resident macrophages or their response to inflammatory stimuli. 2. **[ZNF524](/details-gene/147807) maintains the phenotypic plasticity of vascular smooth muscle cells.** Its high significance in [vascular associated smooth muscle cells](/details-cell/CL0000359) may indicate a role in preserving the contractile phenotype and suppressing the switch to a synthetic, pro-inflammatory phenotype, a key event in vascular diseases like atherosclerosis. ### Experimental Approach To test the hypothesis regarding the role of [ZNF524](/details-gene/147807) in monocyte biology, a loss-of-function study would be highly informative. One could use a CRISPR-Cas9 knockout or CRISPRi-mediated knockdown of [ZNF524](/details-gene/147807) in primary human CD14+ monocytes. These modified cells could then be differentiated into M1 (pro-inflammatory) and M2 (anti-inflammatory) macrophages using standard cytokine cocktails (e.g., LPS/IFN-γ for M1, IL-4/IL-13 for M2). The impact of [ZNF524](/details-gene/147807) depletion on differentiation and polarization could be assessed by measuring changes in cell surface marker expression via flow cytometry, cytokine secretion profiles via ELISA or Luminex, and global transcriptomic shifts using RNA-sequencing. ### Therapeutic Potential As an intracellular DNA-binding transcription factor, [ZNF524](/details-gene/147807) represents a challenging therapeutic target for conventional small molecule inhibitors or biologics. Direct targeting is often hindered by the lack of well-defined binding pockets and the difficulty of achieving specificity. However, if its dysregulation is causally linked to diseases, such as chronic inflammation or vascular proliferative disorders, it could be an important biomarker. Therapeutic strategies might focus on indirectly modulating its activity by targeting more druggable upstream signaling pathways that regulate its expression or downstream effector genes that it controls. Therefore, while not a direct target itself, understanding its regulatory network could unveil more viable therapeutic nodes.

Genular Protein ID: 1907598297

Symbol: ZN524_HUMAN

Name: Zinc finger protein 524

UniProtKB Accession Codes:

Q96C55 Q6NW31 Q96IL7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 DMDM 1 DNASU 1 EMBL 3 Ensembl 2 ExpressionAtlas 1 GO 6 Gene3D 1 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OrthoDB 1 PANTHER 2 PDB 1 PDBsum 1 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 RefSeq 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

Sequence Information:

  • Length: 264
  • Mass: 28709
  • Checksum: B248388F26BBB487
  • Sequence:
    • MDTPSPDPLP SPLPGEEEKP LALSPPVPRG RRGRRPGGAT SSNRTLKASL PRKRGRPPKS 
GQEPPLVQVQ GVTAPVGSSG GSDLLLIDDQ GVPYTVSEGS AAGPEGSGPR KAPHFCPVCL 
RAFPYLSDLE RHSISHSELK PHQCKVCGKT FKRSSHLRRH CNIHAGLRPF RCPLCPRRFR 
EAGELAHHHR VHSGERPYQC PICRLRFTEA NTLRRHAKRK HPEAMGVPLC APDPGSEPPW 
DEEGIPATAG AEEEEETEGK GEPA