Details for: NUSAP1

Gene ID: 51203

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NUSAP1

Ensembl ID: ENSG00000137804

Description: nucleolar and spindle associated protein 1

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • myofibroblast cell CL0000186
    CSI 16.67
    rCSI 23.09%
    PRS 92.79
  • fallopian tube secretory epithelial cell CL4030006
    CSI 16.15
    rCSI 15.55%
    PRS 93.82
  • large pre-B-II cell CL0000957
    CSI 15.09
    rCSI 43.08%
    PRS 94.09
  • promonocyte CL0000559
    CSI 13.12
    rCSI 22.48%
    PRS 95.82
  • neural crest cell CL0011012
    CSI 13.02
    rCSI 10.29%
    PRS 90.75
  • fraction A pre-pro B cell CL0002045
    CSI 11.48
    rCSI 13.14%
    PRS 96.75
  • pro-B cell CL0000826
    CSI 11.43
    rCSI 9.46%
    PRS 95.65
  • enteric smooth muscle cell CL0002504
    CSI 11.33
    rCSI 16.16%
    PRS 94.9
  • goblet cell CL0000160
    CSI 10.16
    rCSI 9.6%
    PRS 93.15
  • plasmablast CL0000980
    CSI 9.88
    rCSI 7.77%
    PRS 95.79
  • mesodermal cell CL0000222
    CSI 8.84
    rCSI 10.61%
    PRS 94.53
  • placental villous trophoblast CL2000060
    CSI 8.07
    rCSI 12.46%
    PRS 93.52
  • myeloid leukocyte CL0000766
    CSI 7.99
    rCSI 7.37%
    PRS 95.88
  • precursor B cell CL0000817
    CSI 7.97
    rCSI 6.98%
    PRS 97.03
  • interstitial cell of Cajal CL0002088
    CSI 7.05
    rCSI 8.97%
    PRS 96.91
  • transit amplifying cell of colon CL0009011
    CSI 6.7
    rCSI 7.87%
    PRS 95.04
  • megakaryocyte CL0000556
    CSI 6.63
    rCSI 28.75%
    PRS 94.8
  • mesenchymal cell CL0008019
    CSI 6.28
    rCSI 15.95%
    PRS 92.05
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 6.28
    rCSI 6.4%
    PRS 97.54
  • double negative thymocyte CL0002489
    CSI 6.07
    rCSI 4.22%
    PRS 98.72
  • granulocyte monocyte progenitor cell CL0000557
    CSI 5.85
    rCSI 5.06%
    PRS 96
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 5.51
    rCSI 4.98%
    PRS 94.22
  • radial glial cell CL0000681
    CSI 5.36
    rCSI 7.45%
    PRS 93.66
  • transit amplifying cell of small intestine CL0009012
    CSI 5.31
    rCSI 23.32%
    PRS 96.52
  • early lymphoid progenitor CL0000936
    CSI 5.17
    rCSI 4.54%
    PRS 96.91
  • erythrocyte CL0000232
    CSI 5.12
    rCSI 11.61%
    PRS 93.16
  • mature B cell CL0000785
    CSI 5.1
    rCSI 4.43%
    PRS 98.09
  • group 3 innate lymphoid cell CL0001071
    CSI 5.04
    rCSI 3.78%
    PRS 96.56
  • primitive red blood cell CL0002355
    CSI 4.81
    rCSI 25.94%
    PRS 95.36
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 4.45
    rCSI 5.14%
    PRS 89.39
  • vascular associated smooth muscle cell CL0000359
    CSI 4.28
    rCSI 13.88%
    PRS 93.81
  • neural cell CL0002319
    CSI 4.28
    rCSI 16.15%
    PRS 84.6
  • keratinocyte CL0000312
    CSI 4.26
    rCSI 3.57%
    PRS 94.54
  • erythroid lineage cell CL0000764
    CSI 3.92
    rCSI 25.25%
    PRS 95.6
  • transit amplifying cell CL0009010
    CSI 3.89
    rCSI 5.96%
    PRS 96.55
  • chondrocyte CL0000138
    CSI 3.81
    rCSI 6.06%
    PRS 91.82
  • germinal center B cell CL0000844
    CSI 3.57
    rCSI 10.64%
    PRS 96.54
  • common myeloid progenitor CL0000049
    CSI 3.44
    rCSI 2.78%
    PRS 95.68
  • neural progenitor cell CL0011020
    CSI 3.37
    rCSI 14.85%
    PRS 86.43
  • thymocyte CL0000893
    CSI 3.25
    rCSI 11.54%
    PRS 98.95
  • respiratory hillock cell CL4030023
    CSI 2.95
    rCSI 5.26%
    PRS 96.73
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.94
    rCSI 2.05%
    PRS 96.84
  • promyelocyte CL0000836
    CSI 2.71
    rCSI 3.92%
    PRS 95.79
  • erythroblast CL0000765
    CSI 2.69
    rCSI 7.13%
    PRS 94.89
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.61
    rCSI 3.35%
    PRS 92.25
  • glioblast CL0000030
    CSI 2.56
    rCSI 4.08%
    PRS 89.43
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 2.42
    rCSI 4.82%
    PRS 98.03
  • extravillous trophoblast CL0008036
    CSI 2.35
    rCSI 2.9%
    PRS 93.5
  • foveolar cell of stomach CL0002179
    CSI 2.35
    rCSI 4.99%
    PRS 95.94
  • respiratory suprabasal cell CL4033048
    CSI 2.3
    rCSI 2.95%
    PRS 95.79
  • intestinal epithelial cell CL0002563
    CSI 2.16
    rCSI 2.26%
    PRS 93.16
  • primordial germ cell CL0000670
    CSI 2.12
    rCSI 10.58%
    PRS 95.63
  • forebrain radial glial cell CL0013000
    CSI 1.94
    rCSI 6.22%
    PRS 94.56
  • erythroid progenitor cell CL0000038
    CSI 1.9
    rCSI 10.89%
    PRS 95.87
  • epithelial cell of nephron CL1000449
    CSI 1.62
    rCSI 15.4%
    PRS 98.88
  • innate lymphoid cell CL0001065
    CSI 1.6
    rCSI 3.31%
    PRS 89.28
  • common dendritic progenitor CL0001029
    CSI 1.59
    rCSI 1.99%
    PRS 97.46
  • pluripotent stem cell CL0002248
    CSI 1.03
    rCSI 30.88%
    PRS 97.2
  • myeloid lineage restricted progenitor cell CL0000839
    CSI 0.93
    rCSI 4.8%
    PRS 98.7
  • eosinophil CL0000771
    CSI 0.76
    rCSI 4.98%
    PRS 98.33
  • myelocyte CL0002193
    CSI 0.68
    rCSI 4.46%
    PRS 97.69
  • epithelial cell of esophagus CL0002252
    CSI 0.63
    rCSI 6.23%
    PRS 92.87
  • pre-conventional dendritic cell CL0002010
    CSI 0.33
    rCSI 4.42%
    PRS 98.56
  • megakaryocyte progenitor cell CL0000553
    CSI 0.32
    rCSI 5.94%
    PRS 98.98

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NUSAP1](/details-gene/51203), or Nucleolar and Spindle Associated Protein 1, is a key regulatory protein critically involved in the execution of mitosis. Its primary function is centered on the organization and stabilization of the mitotic spindle, a microtubule-based structure essential for the accurate segregation of chromosomes during cell division. Functional annotations link it directly to processes such as [mitotic spindle localization](/details-go/GO:0040001), [chromosome condensation](/details-go/GO:0007076), and [sister chromatid segregation](/details-go/GO:0000070). Reflecting its role in cell proliferation, [NUSAP1](/details-gene/51203) shows significant expression in a diverse array of highly proliferative and developing cell types. **Overall**, its highest significance is observed in [myofibroblast cell](/details-cell/CL0000186), various secretory epithelial cells, and a range of B-lymphocyte precursors, including [large pre-B-II cell](/details-cell/CL0000957) and [pro-B cell](/details-cell/CL0000826), highlighting its fundamental importance in tissue development, maintenance, and immune system generation. ## Cellular Roles and Expression Landscape The expression profile of [NUSAP1](/details-gene/51203) underscores its central role in cell cycle progression across multiple lineages. **Overall**, the gene exhibits its highest significance in cells characterized by high rates of proliferation or developmental potential. It is a top marker in [myofibroblast cell](/details-cell/CL0000186) (CSI: 16.67), which are key drivers of tissue repair and fibrosis, and [fallopian tube secretory epithelial cell](/details-cell/CL4030006) (CSI: 16.15), a cell type with high turnover. A prominent feature of its expression pattern is its high significance throughout B-cell development. This includes strong signals in [large pre-B-II cell](/details-cell/CL0000957) (CSI: 15.09), [fraction A pre-pro B cell](/details-cell/CL0002045) (CSI: 11.48), [pro-B cell](/details-cell/CL0000826) (CSI: 11.43), and [precursor B cell](/details-cell/CL0000817) (CSI: 7.97). This pattern suggests that [NUSAP1](/details-gene/51203) is critical for the massive clonal expansion that characterizes B-lymphopoiesis. Furthermore, its high CSI in developmental precursors such as [neural crest cell](/details-cell/CL0011012) (CSI: 13.02), [mesodermal cell](/details-cell/CL0000222) (CSI: 8.84), and [placental villous trophoblast](/details-cell/CL2000060) (CSI: 8.07) reinforces its association with active cell division during embryogenesis and tissue formation. The common thread among these diverse cell types is their proliferative capacity, positioning [NUSAP1](/details-gene/51203) as a general, yet critical, component of the mitotic machinery. ## Pathways and Molecular Function [NUSAP1](/details-gene/51203) functions as a microtubule-associated protein that is essential for the integrity of the mitotic apparatus. Its molecular activities include [microtubule binding](/details-go/GO:0008017) and localizing to key mitotic structures such as the [mitotic spindle](/details-go/GO:0072686) and [chromosomes](/details-go/GO:0005694). Research has shown that it plays a direct role in bundling microtubules and linking them to mitotic chromosomes, a function vital for proper chromosome alignment and segregation ([Link](https://doi.org/10.1083/jcb.200302129); [Link](https://doi.org/10.1016/j.cub.2006.11.050)). The protein's activity is tightly regulated throughout the cell cycle. Its levels peak during mitosis and it is targeted for degradation by the anaphase-promoting complex/cyclosome (APC/C) in a Cdh1-dependent manner as the cell exits mitosis ([Link](https://doi.org/10.1016/j.cellsig.2007.05.017)). Furthermore, its function is modulated by post-translational modifications, including phosphorylation by kinases like ATM, which can induce mitotic arrest in response to cellular stress ([Link](https://doi.org/10.1016/j.bbrc.2010.11.135)). These regulatory mechanisms ensure that its spindle-stabilizing activity is restricted to the correct phase of cell division, preventing genomic instability. ## Research Directions The widespread expression of [NUSAP1](/details-gene/51203) in proliferative cells across different lineages suggests it could be a nodal point in both normal development and pathological conditions like fibrosis and cancer. **Proposed Hypotheses:** 1. Given its top significance in [myofibroblast cell](/details-cell/CL0000186), [NUSAP1](/details-gene/51203) may be a key driver of myofibroblast proliferation in fibrotic diseases. Its overexpression could contribute to the excessive deposition of extracellular matrix characteristic of scarring in organs like the lung, liver, and kidney. 2. The consistent high expression of [NUSAP1](/details-gene/51203) across early B-cell precursors suggests its dysregulation could be a contributing factor in the development or progression of B-cell acute lymphoblastic leukemia (B-ALL) or other B-cell malignancies, which are characterized by uncontrolled proliferation of lymphocyte precursors. **Experimental Approach:** To test the hypothesis regarding its role in fibrosis, one could use an *in vitro* model of myofibroblast differentiation. Primary human lung fibroblasts could be treated with TGF-beta to induce their transition into proliferative, alpha-smooth muscle actin (ACTA2)-positive [myofibroblast cell](/details-cell/CL0000186). The role of [NUSAP1](/details-gene/51203) would be investigated by using siRNA to knock down its expression. The impact of this knockdown on cell proliferation could be quantified using a BrdU incorporation assay, while changes in the fibrotic phenotype could be assessed by measuring the expression of key markers like *ACTA2* and *COL1A1* via RT-qPCR and Western blot. **Therapeutic Potential:** As a protein essential for cell division, [NUSAP1](/details-gene/51203) represents a potential therapeutic target for anti-proliferative strategies, particularly in oncology. **Inhibition** of its function would be the therapeutic goal, as this would be expected to induce mitotic arrest and apoptosis in rapidly dividing cancer cells. Because [NUSAP1](/details-gene/51203) is an intracellular protein, therapeutic development would likely focus on small molecule inhibitors that can disrupt its interaction with microtubules or chromosomes. However, a significant challenge would be potential on-target toxicity in healthy, highly proliferative tissues such as the bone marrow and intestinal epithelium, necessitating careful consideration of the therapeutic window.

Genular Protein ID: 2776596054

Symbol: NUSAP_HUMAN

Name: Nucleolar and spindle-associated protein 1

UniProtKB Accession Codes:

Q9BXS6 B4DDF1 E7ERR5 J3KN21 Q53GW2 Q8TBT4 Q96E58 Q96FJ1 Q9GZM9 Q9NZ85 Q9UI70

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 6 CTD 1 CarbonylDB 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 15 Ensembl 7 ExpressionAtlas 1 GO 13 GeneCards 1 GeneTree 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 1 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 6 Pumba 1 RNAct 1 RefSeq 7 SIGNOR 1 SMR 1 STRING 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 11483580

Title: Gene expression profiling in human fetal liver and identification of tissue- and developmental-stage-specific genes through compiled expression profiles and efficient cloning of full-length cDNAs.

PubMed ID: 11483580

DOI: 10.1101/gr.175501

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 16572171

Title: Analysis of the DNA sequence and duplication history of human chromosome 15.

PubMed ID: 16572171

DOI: 10.1038/nature04601

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 12963707

Title: NuSAP, a novel microtubule-associated protein involved in mitotic spindle organization.

PubMed ID: 12963707

DOI: 10.1083/jcb.200302129

PubMed ID: 17618083

Title: NuSAP is degraded by APC/C-Cdh1 and its overexpression results in mitotic arrest dependent of its microtubules' affinity.

PubMed ID: 17618083

DOI: 10.1016/j.cellsig.2007.05.017

PubMed ID: 17276916

Title: A role for NuSAP in linking microtubules to mitotic chromosomes.

PubMed ID: 17276916

DOI: 10.1016/j.cub.2006.11.050

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21130744

Title: ATM-mediated NuSAP phosphorylation induces mitotic arrest.

PubMed ID: 21130744

DOI: 10.1016/j.bbrc.2010.11.135

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

Sequence Information:

  • Length: 441
  • Mass: 49452
  • Checksum: 1400E1B9F3FB727F
  • Sequence:
    • MIIPSLEELD SLKYSDLQNL AKSLGLRANL RATKLLKALK GYIKHEARKG NENQDESQTS 
ASSCDETEIQ ISNQEEAERQ PLGHVTKTRR RCKTVRVDPD SQQNHSEIKI SNPTEFQNHE 
KQESQDLRAT AKVPSPPDEH QEAENAVSSG NRDSKVPSEG KKSLYTDESS KPGKNKRTAI 
TTPNFKKLHE AHFKEMESID QYIERKKKHF EEHNSMNELK QQPINKGGVR TPVPPRGRLS 
VASTPISQRR SQGRSCGPAS QSTLGLKGSL KRSAISAAKT GVRFSAATKD NEHKRSLTKT 
PARKSAHVTV SGGTPKGEAV LGTHKLKTIT GNSAAVITPF KLTTEATQTP VSNKKPVFDL 
KASLSRPLNY EPHKGKLKPW GQSKENNYLN QHVNRINFYK KTYKQPHLQT KEEQRKKREQ 
ERKEKKAKVL GMRRGLILAE D