Details for: HSPB2

Gene ID: 3316

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: HSPB2

Ensembl ID: ENSG00000170276

Description: heat shock protein family B (small) member 2

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • cardiac muscle cell CL0000746
    CSI 12.29
    rCSI 17.63%
    PRS 79.59
  • muscle cell CL0000187
    CSI 8.37
    rCSI 17.18%
    PRS 92.29
  • microcirculation associated smooth muscle cell CL0008035
    CSI 6.77
    rCSI 19.59%
    PRS 87.3
  • perivascular cell CL4033054
    CSI 6.59
    rCSI 9%
    PRS 91.47
  • skin fibroblast CL0002620
    CSI 4.2
    rCSI 3.62%
    PRS 87.67
  • tracheobronchial smooth muscle cell CL0019019
    CSI 4.2
    rCSI 7.41%
    PRS 91.18
  • alveolar adventitial fibroblast CL4028006
    CSI 3.54
    rCSI 5.59%
    PRS 88.97
  • peripheral nervous system neuron CL2000032
    CSI 3.43
    rCSI 4.67%
    PRS 81.47
  • enteric smooth muscle cell CL0002504
    CSI 3.36
    rCSI 4.79%
    PRS 88.46
  • vascular associated smooth muscle cell CL0000359
    CSI 2.76
    rCSI 8.95%
    PRS 86.17
  • bronchus fibroblast of lung CL2000093
    CSI 2.66
    rCSI 2.16%
    PRS 87.51
  • Schwann cell CL0002573
    CSI 2.52
    rCSI 7.15%
    PRS 84.07
  • alveolar type 1 fibroblast cell CL4028004
    CSI 1.99
    rCSI 2.18%
    PRS 89.56
  • mesenchymal cell CL0008019
    CSI 1.47
    rCSI 3.74%
    PRS 82.73
  • blood vessel smooth muscle cell CL0019018
    CSI 1.01
    rCSI 8.18%
    PRS 84.3
  • bronchiolar smooth muscle cell CL4033017
    CSI 0.54
    rCSI 8.17%
    PRS 93.04

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [HSPB2](/details-gene/3316) (heat shock protein family B (small) member 2) is a protein-coding gene that encodes a member of the small heat shock protein family. These proteins act as molecular chaperones, crucial for preventing protein aggregation and assisting in the refolding of denatured proteins under stress conditions. **Overall**, expression data reveals that [HSPB2](/details-gene/3316) is a highly significant gene in contractile and structural tissues, with its most prominent role observed in [cardiac muscle cell](/details-cell/CL0000746). Its functions in protein quality control, response to cellular stress, and regulation of apoptosis are consistent with its high expression in metabolically active tissues such as muscle. ## Cellular Roles and Expression Landscape The expression profile of [HSPB2](/details-gene/3316) strongly indicates a specialized role in muscular and mesenchymal cell lineages. The gene's highest significance is found in [cardiac muscle cell](/details-cell/CL0000746) (CSI: 12.29), followed by other contractile cell types including generic [muscle cell](/details-cell/CL0000187), [microcirculation associated smooth muscle cell](/details-cell/CL0008035), and [vascular associated smooth muscle cell](/details-cell/CL0000359). This suggests [HSPB2](/details-gene/3316) is a key component of the cellular machinery that maintains proteostasis in tissues subjected to high mechanical and metabolic stress. Beyond muscle, [HSPB2](/details-gene/3316) also shows significant expression in associated structural and stromal cells. These include [perivascular cell](/details-cell/CL4033054), various fibroblast populations such as [skin fibroblast](/details-cell/CL0002620) and [alveolar adventitial fibroblast](/details-cell/CL4028006), and [mesenchymal cell](/details-cell/CL0008019). Its presence in [Schwann cell](/details-cell/CL0002573) and [peripheral nervous system neuron](/details-cell/CL2000032) points to a broader, albeit less prominent, role in protecting the nervous system from cellular stress. The collective data portrays [HSPB2](/details-gene/3316) as a guardian of cellular integrity primarily within the musculoskeletal and cardiovascular systems. ## Pathways and Molecular Function Functionally, [HSPB2](/details-gene/3316) is deeply integrated into the cellular stress response network. Gene Ontology annotations highlight its role in [protein refolding](/details-go/GO:0042026), binding to unfolded proteins ([GO:0051082](https://www.ebi.ac.uk/QuickGO/term/GO:0051082)), and negatively regulating the apoptotic process ([GO:0043066](https://www.ebi.ac.uk/QuickGO/term/GO:0043066)). These functions are central to its identity as a heat shock protein. Reactome pathway analysis further refines this role, placing [HSPB2](/details-gene/3316) as a key player in the [Cellular response to heat stress](/details-pathway/R-HSA-3371556). It is involved in processes downstream of the master heat shock regulator, HSF1, including [Hsf1 activation](/details-pathway/R-HSA-3371511) and [Hsf1-dependent transactivation](/details-pathway/R-HSA-3371571). This pathway involvement is directly aligned with its high expression in [cardiac muscle cell](/details-cell/CL0000746), where a robust stress response is critical for survival during events like ischemia. Furthermore, its annotation for [somatic muscle development](/details-go/GO:0007525) and its documented interaction with myotonic dystrophy protein kinase suggest a direct role in muscle physiology beyond just stress response ([Link](https://doi.org/10.1083/jcb.140.5.1113)). ## Research Directions The specific and high-level expression of [HSPB2](/details-gene/3316) in muscle tissues, coupled with its role as a molecular chaperone, suggests it is a critical factor in maintaining muscle health and responding to injury. ### Proposed Hypotheses: 1. **Cardioprotective Role:** Given its premier expression in [cardiac muscle cell](/details-cell/CL0000746) and its anti-apoptotic function, we hypothesize that [HSPB2](/details-gene/3316) is a crucial protective factor against ischemia-reperfusion injury in the heart, where it mitigates protein aggregation and cell death. 2. **Role in Myopathy:** The established link to myotonic dystrophy protein kinase ([Link](https://doi.org/10.1083/jcb.140.5.1113)) and its high expression across multiple muscle types suggest that loss-of-function or dysregulation of [HSPB2](/details-gene/3316) may contribute to the pathology of a broader range of inherited or acquired myopathies by compromising the muscle's ability to cope with proteotoxic stress. ### Experimental Approach: To test the hypothesis of a cardioprotective role for [HSPB2](/details-gene/3316), a preclinical study could be designed using a mouse model. [HSPB2](/details-gene/3316) knockout and wild-type mice would be subjected to surgically induced myocardial infarction (MI). The functional consequences could be assessed via echocardiography to measure cardiac output and ejection fraction. At the molecular level, heart tissue from the infarct and border zones would be analyzed post-MI for markers of apoptosis (e.g., cleaved caspase-3, TUNEL staining) and protein aggregation (e.g., ubiquitin-positive inclusion bodies) to determine if the absence of [HSPB2](/details-gene/3316) exacerbates cellular damage. ### Therapeutic Potential: As a cytoprotective chaperone, [HSPB2](/details-gene/3316) represents a potential therapeutic target where **activation** or **upregulation** would be the desired outcome. For conditions like ischemic heart disease or certain muscular dystrophies characterized by protein aggregation, enhancing [HSPB2](/details-gene/3316) activity could be beneficial. However, as an intracellular protein, it is a challenging target for traditional small molecules or antibody-based therapies. A more viable long-term strategy might involve gene therapy to restore its expression in affected muscle tissue or the development of pharmacological chaperones or small molecules that specifically induce its expression via the HSF1 pathway.

Genular Protein ID: 173642965

Symbol: HSPB2_HUMAN

Name: Heat shock protein beta-2

UniProtKB Accession Codes:

Q16082 Q6I9U7

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 6 Ensembl 1 GO 11 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 1 PDBsum 1 PRINTS 1 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 RefSeq 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 9344664

Title: Identification and characterization of the gene encoding a new member of the alpha-crystallin/small hsp family, closely linked to the alphaB-crystallin gene in a head-to-head manner.

PubMed ID: 9344664

DOI: 10.1006/geno.1997.4956

PubMed ID: 9490724

Title: MKBP, a novel member of the small heat shock protein family, binds and activates the myotonic dystrophy protein kinase.

PubMed ID: 9490724

DOI: 10.1083/jcb.140.5.1113

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 8227983

Title: Muscle autoantigens in thyroid associated ophthalmopathy: the limits of molecular genetics.

PubMed ID: 8227983

DOI: 10.1007/bf03348900

PubMed ID: 19464326

Title: HSPB7 is a SC35 speckle resident small heat shock protein.

PubMed ID: 19464326

DOI: 10.1016/j.bbamcr.2009.05.005

Sequence Information:

  • Length: 182
  • Mass: 20233
  • Checksum: 7586DCE8FBD19F6D
  • Sequence:
    • MSGRSVPHAH PATAEYEFAN PSRLGEQRFG EGLLPEEILT PTLYHGYYVR PRAAPAGEGS 
RAGASELRLS EGKFQAFLDV SHFTPDEVTV RTVDNLLEVS ARHPQRLDRH GFVSREFCRT 
YVLPADVDPW RVRAALSHDG ILNLEAPRGG RHLDTEVNEV YISLLPAPPD PEEEEEAAIV 
EP