Details for: PIN1

Gene ID: 5300

Symbol: PIN1

Ensembl ID: ENSG00000127445

Description: peptidylprolyl cis/trans isomerase, NIMA-interacting 1

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 249.4451
    Cell Significance Index: -38.8000
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 171.9343
    Cell Significance Index: -43.6100
  • Cell Name: embryonic stem cell (CL0002322)
    Fold Change: 138.5613
    Cell Significance Index: -57.0800
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 111.7136
    Cell Significance Index: -52.7400
  • Cell Name: peripheral blood mononuclear cell (CL2000001)
    Fold Change: 97.9810
    Cell Significance Index: -50.4000
  • Cell Name: ileal goblet cell (CL1000326)
    Fold Change: 79.1628
    Cell Significance Index: -53.1200
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 41.8101
    Cell Significance Index: -51.5500
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 13.8214
    Cell Significance Index: -54.5400
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 12.3427
    Cell Significance Index: -37.9100
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 9.0561
    Cell Significance Index: -24.2600
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 3.2870
    Cell Significance Index: 172.5800
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 3.0719
    Cell Significance Index: 212.4400
  • Cell Name: gut absorptive cell (CL0000677)
    Fold Change: 1.7097
    Cell Significance Index: 102.6400
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 1.5608
    Cell Significance Index: 45.8400
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: 1.4440
    Cell Significance Index: 37.9700
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 1.4007
    Cell Significance Index: 90.3700
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: 1.3678
    Cell Significance Index: 28.6300
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 1.3285
    Cell Significance Index: 725.5400
  • Cell Name: skeletal muscle myoblast (CL0000515)
    Fold Change: 1.3181
    Cell Significance Index: 14.3300
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 1.2673
    Cell Significance Index: 174.0400
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: 1.1643
    Cell Significance Index: 40.4600
  • Cell Name: kidney cell (CL1000497)
    Fold Change: 1.1122
    Cell Significance Index: 8.8800
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.9723
    Cell Significance Index: 114.6600
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.9229
    Cell Significance Index: 113.4800
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.8726
    Cell Significance Index: 157.3100
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.7398
    Cell Significance Index: 46.6300
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.6646
    Cell Significance Index: 133.3200
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.6549
    Cell Significance Index: 30.5400
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.6400
    Cell Significance Index: 282.9700
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: 0.5507
    Cell Significance Index: 41.0400
  • Cell Name: odontoblast (CL0000060)
    Fold Change: 0.5419
    Cell Significance Index: 69.4700
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.5232
    Cell Significance Index: 99.5600
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.5013
    Cell Significance Index: 179.8100
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: 0.4770
    Cell Significance Index: 22.4200
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.4560
    Cell Significance Index: 45.1100
  • Cell Name: cone retinal bipolar cell (CL0000752)
    Fold Change: 0.4126
    Cell Significance Index: 3.1800
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.3619
    Cell Significance Index: 16.4100
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 0.3311
    Cell Significance Index: 17.2000
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 0.2961
    Cell Significance Index: 20.9400
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 0.2878
    Cell Significance Index: 37.1800
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.2058
    Cell Significance Index: 40.8500
  • Cell Name: endothelial cell of venule (CL1000414)
    Fold Change: 0.1981
    Cell Significance Index: 2.2500
  • Cell Name: retinal rod cell (CL0000604)
    Fold Change: 0.1007
    Cell Significance Index: 1.2000
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.0484
    Cell Significance Index: 7.8800
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.0415
    Cell Significance Index: 0.7000
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: 0.0210
    Cell Significance Index: 15.8600
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0017
    Cell Significance Index: -1.2800
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: -0.0142
    Cell Significance Index: -0.4100
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0144
    Cell Significance Index: -27.1500
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: -0.0262
    Cell Significance Index: -0.9200
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.0262
    Cell Significance Index: -0.7000
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0268
    Cell Significance Index: -2.7400
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0303
    Cell Significance Index: -55.8300
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0376
    Cell Significance Index: -57.8400
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0425
    Cell Significance Index: -31.4900
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0428
    Cell Significance Index: -58.2600
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: -0.0452
    Cell Significance Index: -31.2700
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: -0.0519
    Cell Significance Index: -3.4900
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: -0.0549
    Cell Significance Index: -34.8900
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0758
    Cell Significance Index: -42.7600
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0878
    Cell Significance Index: -39.8500
  • Cell Name: paneth cell of colon (CL0009009)
    Fold Change: -0.0881
    Cell Significance Index: -1.3200
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0991
    Cell Significance Index: -61.8900
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: -0.1274
    Cell Significance Index: -21.7600
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.1554
    Cell Significance Index: -44.7000
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.1668
    Cell Significance Index: -4.5400
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: -0.1764
    Cell Significance Index: -10.8400
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.2320
    Cell Significance Index: -27.0400
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: -0.2360
    Cell Significance Index: -6.0300
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: -0.2766
    Cell Significance Index: -5.8900
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.2798
    Cell Significance Index: -58.9300
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.2922
    Cell Significance Index: -42.4700
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.3033
    Cell Significance Index: -34.7500
  • Cell Name: cerebellar granule cell (CL0001031)
    Fold Change: -0.3326
    Cell Significance Index: -5.7000
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.3591
    Cell Significance Index: -11.5000
  • Cell Name: umbrella cell of urothelium (CL4030056)
    Fold Change: -0.3660
    Cell Significance Index: -3.3700
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: -0.3754
    Cell Significance Index: -10.4900
  • Cell Name: peg cell (CL4033014)
    Fold Change: -0.4012
    Cell Significance Index: -9.2700
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.4188
    Cell Significance Index: -32.1400
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: -0.4213
    Cell Significance Index: -11.2700
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.4473
    Cell Significance Index: -35.4300
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: -0.4691
    Cell Significance Index: -8.6700
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.5221
    Cell Significance Index: -54.3600
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: -0.5267
    Cell Significance Index: -11.4100
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: -0.5452
    Cell Significance Index: -30.6000
  • Cell Name: vascular lymphangioblast (CL0005022)
    Fold Change: -0.5605
    Cell Significance Index: -9.9100
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: -0.5897
    Cell Significance Index: -6.1100
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: -0.6348
    Cell Significance Index: -28.0800
  • Cell Name: hippocampal pyramidal neuron (CL1001571)
    Fold Change: -0.7256
    Cell Significance Index: -20.7100
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: -0.7384
    Cell Significance Index: -21.7500
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: -0.7608
    Cell Significance Index: -20.3900
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: -0.7987
    Cell Significance Index: -30.2500
  • Cell Name: microcirculation associated smooth muscle cell (CL0008035)
    Fold Change: -0.8417
    Cell Significance Index: -7.0700
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: -0.8617
    Cell Significance Index: -24.7000
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.8618
    Cell Significance Index: -52.8400
  • Cell Name: interstitial cell of ovary (CL0002094)
    Fold Change: -0.9206
    Cell Significance Index: -11.7900
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: -0.9257
    Cell Significance Index: -12.6300
  • Cell Name: foveolar cell of stomach (CL0002179)
    Fold Change: -1.0775
    Cell Significance Index: -7.0300
  • Cell Name: pvalb GABAergic cortical interneuron (CL4023018)
    Fold Change: -1.1251
    Cell Significance Index: -23.8800
  • Cell Name: mesodermal cell (CL0000222)
    Fold Change: -1.1725
    Cell Significance Index: -7.3400

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics** PIN1 is a peptidyl-prolyl cis/trans isomerase that belongs to the PIN family of proteins. Its primary function is to catalyze the cis-trans isomerization of peptide bonds, which is critical for protein folding and stability. PIN1's ability to interact with various proteins, including NIMA kinase, suggests its involvement in multiple cellular processes. Its expression in multiple cell types and its role in regulating protein stability, localization, and phosphorylation make it a key regulator of cellular homeostasis. **Pathways and Functions** PIN1's involvement in various cellular processes can be attributed to its ability to regulate protein stability, localization, and phosphorylation. Its interactions with proteins such as NIMA kinase, TP53, and rho GTPases suggest its role in cell cycle regulation, apoptosis, and signaling pathways. PIN1's expression in immune cells, neurons, and epithelial cells highlights its importance in maintaining tissue homeostasis and immune function. Some of the key pathways and functions associated with PIN1 include: 1. **Cell cycle regulation**: PIN1's interaction with NIMA kinase suggests its involvement in regulating the G2/M phase of the cell cycle. 2. **Apoptosis**: PIN1's regulation of TP53 activity and its interaction with rho GTPases suggest its role in regulating apoptosis. 3. **Signaling pathways**: PIN1's interaction with rho GTPases and its regulation of protein phosphorylation suggest its involvement in signaling pathways. 4. **Protein stability and localization**: PIN1's ability to regulate protein stability and localization suggests its role in maintaining cellular homeostasis. **Clinical Significance** PIN1's dysregulation has been implicated in various diseases, including cancer, neurodegenerative disorders, and autoimmune diseases. Its role in regulating protein stability, localization, and phosphorylation makes it a potential target for therapeutic interventions. Some of the potential clinical applications of PIN1 include: 1. **Cancer therapy**: PIN1's dysregulation in cancer cells makes it a potential target for cancer therapy. 2. **Neurodegenerative disorders**: PIN1's role in regulating protein stability and localization suggests its involvement in neurodegenerative disorders such as Alzheimer's and Parkinson's disease. 3. **Autoimmune diseases**: PIN1's interaction with immune cells and its regulation of protein stability and localization suggest its role in autoimmune diseases such as rheumatoid arthritis and multiple sclerosis. In conclusion, PIN1 is a highly conserved protein that plays a crucial role in various cellular processes. Its dysregulation has been implicated in various diseases, and its potential as a therapeutic target makes it an area of active research.

Genular Protein ID: 1232103284

Symbol: PIN1_HUMAN

Name: Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1

UniProtKB Accession Codes:

Q13526 A8K4V9 Q53X75

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BMRB 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CORUM 1 CTD 1 ChEBI 2 ChEMBL 1 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DisProt 1 DrugBank 2 DrugCentral 1 EC 1 ELM 1 EMBL 6 Ensembl 2 EvolutionaryTrace 1 ExpressionAtlas 1 GO 44 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 GuidetoPHARMACOLOGY 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 100 PDBsum 100 PIR 1 PRO 1 PROSITE 4 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 5 RefSeq 1 Rhea 3 SIGNOR 1 SMART 1 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8606777

Title: A human peptidyl-prolyl isomerase essential for regulation of mitosis.

PubMed ID: 8606777

DOI: 10.1038/380544a0

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 11470801

Title: Identification of a novel kinesin-related protein, KRMP1, as a target for mitotic peptidyl-prolyl isomerase Pin1.

PubMed ID: 11470801

DOI: 10.1074/jbc.m106207200

PubMed ID: 15664191

Title: Regulation of Raf-1 by direct feedback phosphorylation.

PubMed ID: 15664191

DOI: 10.1016/j.molcel.2004.11.055

PubMed ID: 16476580

Title: Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients.

PubMed ID: 16476580

DOI: 10.1016/j.bbrc.2005.12.228

PubMed ID: 16644721

Title: Regulation of Bruton tyrosine kinase by the peptidylprolyl isomerase Pin1.

PubMed ID: 16644721

DOI: 10.1074/jbc.m603090200

PubMed ID: 17828269

Title: Genotoxic stress regulates expression of the proto-oncogene Bcl6 in germinal center B cells.

PubMed ID: 17828269

DOI: 10.1038/ni1508

PubMed ID: 17525332

Title: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.

PubMed ID: 17525332

DOI: 10.1126/science.1140321

PubMed ID: 18628984

Title: Nerve growth factor stimulates interaction of Cayman ataxia protein BNIP-H/Caytaxin with peptidyl-prolyl isomerase Pin1 in differentiating neurons.

PubMed ID: 18628984

DOI: 10.1371/journal.pone.0002686

PubMed ID: 19367327

Title: Protein kinase-X interacts with Pin-1 and Polycystin-1 during mouse kidney development.

PubMed ID: 19367327

DOI: 10.1038/ki.2009.95

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 22033920

Title: Mitogen-activated protein kinase extracellular signal-regulated kinase 2 phosphorylates and promotes Pin1 protein-dependent promyelocytic leukemia protein turnover.

PubMed ID: 22033920

DOI: 10.1074/jbc.m111.289512

PubMed ID: 21497122

Title: Death-associated protein kinase 1 phosphorylates Pin1 and inhibits its prolyl isomerase activity and cellular function.

PubMed ID: 21497122

DOI: 10.1016/j.molcel.2011.03.005

PubMed ID: 22608923

Title: Negative regulation of the stability and tumor suppressor function of Fbw7 by the Pin1 prolyl isomerase.

PubMed ID: 22608923

DOI: 10.1016/j.molcel.2012.04.012

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 23623683

Title: Prolyl isomerase PIN1 regulates DNA double-strand break repair by counteracting DNA end resection.

PubMed ID: 23623683

DOI: 10.1016/j.molcel.2013.03.023

PubMed ID: 27561354

Title: Cullin3-KLHL15 ubiquitin ligase mediates CtIP protein turnover to fine-tune DNA-end resection.

PubMed ID: 27561354

DOI: 10.1038/ncomms12628

PubMed ID: 29686383

Title: The IL-33-PIN1-IRAK-M axis is critical for type 2 immunity in IL-33-induced allergic airway inflammation.

PubMed ID: 29686383

DOI: 10.1038/s41467-018-03886-6

PubMed ID: 9200606

Title: Structural and functional analysis of the mitotic rotamase Pin1 suggests substrate recognition is phosphorylation dependent.

PubMed ID: 9200606

DOI: 10.1016/s0092-8674(00)80273-1

Sequence Information:

  • Length: 163
  • Mass: 18243
  • Checksum: 35391AF40B7D1E13
  • Sequence:
    • MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL 
LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG 
DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.