Details for: NOB1

Gene ID: 28987

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: NOB1

Ensembl ID: ENSG00000141101

Description: NIN1 (RPN12) binding protein 1 homolog

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 10.4
    rCSI 9.39%
    PRS 68.97
  • mature B cell CL0000785
    CSI 6.82
    rCSI 5.93%
    PRS 82.04
  • respiratory basal cell CL0002633
    CSI 6.73
    rCSI 6.98%
    PRS 76.97
  • pro-B cell CL0000826
    CSI 5.96
    rCSI 4.94%
    PRS 74.04
  • intestinal epithelial cell CL0002563
    CSI 5.83
    rCSI 6.09%
    PRS 69.33
  • activated type II NK T cell CL0000931
    CSI 5.8
    rCSI 6.53%
    PRS 86.36
  • fibroblast of lung CL0002553
    CSI 5.29
    rCSI 4.92%
    PRS 72.45
  • common myeloid progenitor CL0000049
    CSI 5.19
    rCSI 4.19%
    PRS 73.64
  • CD4-positive, alpha-beta thymocyte CL0000810
    CSI 4.75
    rCSI 3.81%
    PRS 88.32
  • naive T cell CL0000898
    CSI 4.71
    rCSI 3.28%
    PRS 85.96
  • pancreatic acinar cell CL0002064
    CSI 4.66
    rCSI 6.19%
    PRS 77.92
  • lung macrophage CL1001603
    CSI 4.45
    rCSI 9.95%
    PRS 79.5
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 4.21
    rCSI 4.14%
    PRS 86.15
  • club cell CL0000158
    CSI 4.18
    rCSI 6.12%
    PRS 66.52
  • stem cell CL0000034
    CSI 4.01
    rCSI 3.87%
    PRS 63.54
  • group 3 innate lymphoid cell CL0001071
    CSI 3.9
    rCSI 2.93%
    PRS 77.56
  • double negative thymocyte CL0002489
    CSI 3.82
    rCSI 2.66%
    PRS 83.09
  • neural crest cell CL0011012
    CSI 3.72
    rCSI 2.94%
    PRS 59.02
  • erythroid progenitor cell CL0000038
    CSI 3.68
    rCSI 21.08%
    PRS 79.12
  • effector CD8-positive, alpha-beta T cell CL0001050
    CSI 3.54
    rCSI 2.69%
    PRS 84.34
  • effector CD4-positive, alpha-beta T cell CL0001044
    CSI 3.45
    rCSI 9.88%
    PRS 89.43
  • plasmablast CL0000980
    CSI 3.35
    rCSI 2.64%
    PRS 78.12
  • T follicular helper cell CL0002038
    CSI 3.2
    rCSI 2.39%
    PRS 85.57
  • mature alpha-beta T cell CL0000791
    CSI 3.12
    rCSI 11.31%
    PRS 88.43
  • perivascular cell CL4033054
    CSI 3.05
    rCSI 4.18%
    PRS 77.12
  • early lymphoid progenitor CL0000936
    CSI 3
    rCSI 2.64%
    PRS 77.07
  • basal-myoepithelial cell of mammary gland CL0002324
    CSI 2.94
    rCSI 5.56%
    PRS 85.85
  • ciliated epithelial cell CL0000067
    CSI 2.84
    rCSI 2.5%
    PRS 59.83
  • extravillous trophoblast CL0008036
    CSI 2.71
    rCSI 3.35%
    PRS 68.82
  • skin fibroblast CL0002620
    CSI 2.65
    rCSI 2.28%
    PRS 74.81
  • CD8-positive, alpha-beta cytotoxic T cell CL0000794
    CSI 2.63
    rCSI 3.14%
    PRS 88.49
  • transit amplifying cell CL0009010
    CSI 2.57
    rCSI 3.94%
    PRS 82.63
  • epithelial cell of lower respiratory tract CL0002632
    CSI 2.53
    rCSI 1.96%
    PRS 74.98
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.46
    rCSI 1.72%
    PRS 74.69
  • interneuron CL0000099
    CSI 2.45
    rCSI 4.92%
    PRS 60.93
  • acinar cell CL0000622
    CSI 2.43
    rCSI 3.56%
    PRS 82.47
  • myeloid leukocyte CL0000766
    CSI 2.4
    rCSI 2.22%
    PRS 73.25
  • IgA plasma cell CL0000987
    CSI 2.35
    rCSI 2.41%
    PRS 82.57
  • hematopoietic stem cell CL0000037
    CSI 2.32
    rCSI 1.55%
    PRS 74.51
  • effector memory CD8-positive, alpha-beta T cell, terminally differentiated CL0001062
    CSI 2.28
    rCSI 11.46%
    PRS 83.97
  • hematopoietic precursor cell CL0008001
    CSI 2.28
    rCSI 2.34%
    PRS 85.65
  • CD4-positive helper T cell CL0000492
    CSI 2.26
    rCSI 1.71%
    PRS 84.8
  • bronchus fibroblast of lung CL2000093
    CSI 2.24
    rCSI 1.82%
    PRS 71.68
  • myofibroblast cell CL0000186
    CSI 2.21
    rCSI 3.05%
    PRS 70.62
  • epithelial cell of lung CL0000082
    CSI 2.14
    rCSI 1.78%
    PRS 71.83
  • enteric smooth muscle cell CL0002504
    CSI 2.13
    rCSI 3.04%
    PRS 73.29
  • transit amplifying cell of colon CL0009011
    CSI 2.11
    rCSI 2.48%
    PRS 73.78
  • mesodermal cell CL0000222
    CSI 2.07
    rCSI 2.48%
    PRS 69.7
  • mucous neck cell CL0000651
    CSI 2.05
    rCSI 2.95%
    PRS 80.52
  • epithelial cell CL0000066
    CSI 2.03
    rCSI 3.13%
    PRS 63.86
  • keratinocyte CL0000312
    CSI 2
    rCSI 1.67%
    PRS 75.05
  • granulocyte monocyte progenitor cell CL0000557
    CSI 1.99
    rCSI 1.72%
    PRS 76.33
  • colon epithelial cell CL0011108
    CSI 1.97
    rCSI 2.07%
    PRS 68.63
  • fraction A pre-pro B cell CL0002045
    CSI 1.97
    rCSI 2.26%
    PRS 86.12
  • intestine goblet cell CL0019031
    CSI 1.93
    rCSI 1.72%
    PRS 69.51
  • goblet cell CL0000160
    CSI 1.93
    rCSI 1.83%
    PRS 70.89
  • multi-ciliated epithelial cell CL0005012
    CSI 1.92
    rCSI 1.91%
    PRS 65.18
  • fallopian tube secretory epithelial cell CL4030006
    CSI 1.86
    rCSI 1.79%
    PRS 71.27
  • CD4-positive, alpha-beta cytotoxic T cell CL0000934
    CSI 1.85
    rCSI 2.54%
    PRS 88.88
  • placental villous trophoblast CL2000060
    CSI 1.82
    rCSI 2.81%
    PRS 70.55
  • immature B cell CL0000816
    CSI 1.78
    rCSI 1.32%
    PRS 83.96
  • large pre-B-II cell CL0000957
    CSI 1.76
    rCSI 5.04%
    PRS 80.16
  • alveolar adventitial fibroblast CL4028006
    CSI 1.74
    rCSI 2.75%
    PRS 73.6
  • peripheral nervous system neuron CL2000032
    CSI 1.74
    rCSI 2.37%
    PRS 62.94
  • vascular associated smooth muscle cell CL0000359
    CSI 1.71
    rCSI 5.54%
    PRS 70.62
  • promyelocyte CL0000836
    CSI 1.7
    rCSI 2.45%
    PRS 79.4
  • radial glial cell CL0000681
    CSI 1.68
    rCSI 2.33%
    PRS 70.21
  • kidney epithelial cell CL0002518
    CSI 1.63
    rCSI 3.12%
    PRS 87.73
  • CD8-positive, CD28-negative, alpha-beta regulatory T cell CL0000920
    CSI 1.61
    rCSI 3.21%
    PRS 86
  • basal cell CL0000646
    CSI 1.6
    rCSI 2.14%
    PRS 71.26
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 1.6
    rCSI 2.83%
    PRS 51.89
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 1.59
    rCSI 2.04%
    PRS 68.19
  • mesenchymal cell CL0008019
    CSI 1.57
    rCSI 4%
    PRS 65.25
  • ependymal cell CL0000065
    CSI 1.56
    rCSI 3.17%
    PRS 49.75
  • paneth cell CL0000510
    CSI 1.55
    rCSI 2.29%
    PRS 84.68
  • basal cell of prostate epithelium CL0002341
    CSI 1.5
    rCSI 4.33%
    PRS 80.66
  • common dendritic progenitor CL0001029
    CSI 1.5
    rCSI 1.88%
    PRS 81.56
  • pancreatic ductal cell CL0002079
    CSI 1.44
    rCSI 2.81%
    PRS 75.08
  • glioblast CL0000030
    CSI 1.31
    rCSI 2.09%
    PRS 63.39
  • common lymphoid progenitor CL0000051
    CSI 1.24
    rCSI 1.66%
    PRS 88.87
  • thymocyte CL0000893
    CSI 1.17
    rCSI 4.15%
    PRS 93.83
  • retinal cone cell CL0000573
    CSI 1.13
    rCSI 1.82%
    PRS 61.01
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 1.02
    rCSI 2.44%
    PRS 86.71
  • stromal cell of ovary CL0002132
    CSI 1.01
    rCSI 2.78%
    PRS 81.13
  • mammary gland epithelial cell CL0002327
    CSI 1.01
    rCSI 3.53%
    PRS 81.76
  • forebrain radial glial cell CL0013000
    CSI 0.98
    rCSI 3.15%
    PRS 75.04
  • granulocyte CL0000094
    CSI 0.91
    rCSI 1.4%
    PRS 80.35
  • myeloid lineage restricted progenitor cell CL0000839
    CSI 0.85
    rCSI 4.36%
    PRS 89.57
  • primitive red blood cell CL0002355
    CSI 0.78
    rCSI 4.21%
    PRS 80.74
  • microcirculation associated smooth muscle cell CL0008035
    CSI 0.71
    rCSI 2.07%
    PRS 72.1
  • basal cell of epithelium of trachea CL1000348
    CSI 0.67
    rCSI 4.73%
    PRS 80.64
  • vein endothelial cell of respiratory system CL4033008
    CSI 0.62
    rCSI 4.26%
    PRS 81.58
  • endothelial cell of placenta CL0009092
    CSI 0.62
    rCSI 3.04%
    PRS 81.53
  • pancreatic stellate cell CL0002410
    CSI 0.56
    rCSI 3.23%
    PRS 78.09
  • pluripotent stem cell CL0002248
    CSI 0.2
    rCSI 5.87%
    PRS 86.53
  • megakaryocyte progenitor cell CL0000553
    CSI 0.19
    rCSI 3.47%
    PRS 92.58

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [NOB1](/details-gene/28987) encodes the NIN1 (RPN12) binding protein 1 homolog, an RNA-binding protein that plays a critical role in ribosome biogenesis. Functionally, it is an endonuclease involved in the maturation of the small ribosomal subunit, a fundamental process for all protein synthesis. Its expression profile reflects this core cellular function, showing high significance in a wide range of highly proliferative or metabolically active cell types. **Overall**, [NOB1](/details-gene/28987) is particularly prominent in hematopoietic progenitors, developing B cells, and regenerating epithelial tissues, suggesting its importance in cell growth, differentiation, and tissue maintenance. ## Cellular Roles and Expression Landscape The expression pattern of [NOB1](/details-gene/28987) underscores its essential role in cellular proliferation and protein production. **Overall**, the gene shows the highest significance in progenitor cell populations, including [megakaryocyte-erythroid progenitor cell](/details-cell/CL0000050) (CSI: 10.40) and [common myeloid progenitor](/details-cell/CL0000049) (CSI: 5.19), highlighting its requirement during the early stages of hematopoiesis. This theme extends to the lymphoid lineage, with high significance in [mature B cell](/details-cell/CL0000785) (CSI: 6.82) and their precursors, [pro-B cell](/details-cell/CL0000826) (CSI: 5.96), as well as in various T cell subsets such as [activated type II NK T cell](/details-cell/CL0000931) (CSI: 5.80) and [CD4-positive, alpha-beta thymocyte](/details-cell/CL0000810) (CSI: 4.75). This is consistent with the high demand for protein synthesis during immune cell development, activation, and effector function. Beyond the hematopoietic system, [NOB1](/details-gene/28987) is also highly significant in tissues characterized by rapid cell turnover or high secretory activity. This includes [respiratory basal cell](/details-cell/CL0002633) (CSI: 6.73), [intestinal epithelial cell](/details-cell/CL0002563) (CSI: 5.83), and [pancreatic acinar cell](/details-cell/CL0002064) (CSI: 4.66). Its presence in [stem cell](/details-cell/CL0000034) (CSI: 4.01) populations further reinforces its association with cellular proliferation and self-renewal. ## Pathways and Molecular Function The functional annotations for [NOB1](/details-gene/28987) align precisely with its role as a key factor in ribosome synthesis. It is a central component of biological processes such as `[Maturation of ssu-rrna](/ontology/GO:0030490)` and `[rRNA processing](/ontology/GO:0006364)`. This is further detailed in Reactome pathways, including `[Major pathway of rRNA processing in the nucleolus and cytosol](/reactome/R-HSA-6791226)` and `[rRNA processing in the nucleus and cytosol](/reactome/R-HSA-8868773)`. Its molecular functions, which include `[RNA endonuclease activity](/ontology/GO:0004521)`, `[metal ion binding](/ontology/GO:0046872)`, and `[protein binding](/ontology/GO:0005515)`, describe the biochemical activities required to process ribosomal RNA precursors. Consistent with this function, the protein localizes to the `[cytosol](/ontology/GO:0005829)`, `[nucleoplasm](/ontology/GO:0005654)`, and specifically to the `[preribosome, small subunit precursor](/ontology/GO:0030688)`, the site of ribosome assembly. One study identified and characterized the human [NOB1](/details-gene/28987) gene, confirming its role in these fundamental cellular processes ([Link](https://doi.org/10.1007/s11033-005-3141-7)). ## Research Directions Given its fundamental role in ribosome biogenesis, a process frequently dysregulated in cancer, the differential regulation and requirement for [NOB1](/details-gene/28987) in malignant versus healthy proliferating cells presents a key area for investigation. **Testable Hypotheses:** 1. The high significance of [NOB1](/details-gene/28987) in hematopoietic progenitors suggests that its activity is a critical rate-limiting step for normal blood cell development. Consequently, its dysregulation may be a contributing factor in the pathogenesis of hematological malignancies such as leukemia, where ribosome biogenesis is often upregulated. 2. In rapidly renewing epithelial tissues, such as the lung and gut, [NOB1](/details-gene/28987) expression in progenitor cells like the [respiratory basal cell](/details-cell/CL0002633) is essential for tissue regeneration after injury. Reduced [NOB1](/details-gene/28987) function could impair repair mechanisms and contribute to chronic inflammatory or fibrotic diseases. **Proposed Experiment:** To test the first hypothesis, a conditional knockout of [NOB1](/details-gene/28987) could be generated in a mouse hematopoietic system. Deletion of the gene in adult hematopoietic stem cells would allow for the assessment of its impact on the maintenance of progenitor pools and their differentiation into various blood lineages *in vivo*. Bone marrow from these mice could be analyzed by flow cytometry and colony-forming assays to precisely quantify defects in myeloid and lymphoid development, directly testing the necessity of [NOB1](/details-gene/28987) for hematopoiesis. **Therapeutic Potential:** As a crucial enzyme in the universally essential pathway of ribosome biogenesis, [NOB1](/details-gene/28987) represents a potential target for anti-proliferative therapies, particularly in oncology. Inhibition of its endonuclease activity could selectively halt the growth of rapidly dividing cancer cells that have an elevated demand for protein synthesis. However, its widespread importance in healthy progenitor and renewing tissues suggests a high risk of on-target toxicity, such as myelosuppression and mucositis. Therefore, therapeutic strategies would likely require sophisticated drug delivery systems (e.g., antibody-drug conjugates) to specifically target cancer cells and achieve an acceptable therapeutic window.

Genular Protein ID: 2693032315

Symbol: NOB1_HUMAN

Name: RNA-binding protein NOB1

UniProtKB Accession Codes:

Q9ULX3 Q7L6B7 Q7M4M4 Q7Z4B5 Q9NWB0

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChEBI 4 DMDM 1 DNASU 1 DisGeNET 1 EMBL 6 EMDB 9 Ensembl 1 ExpressionAtlas 1 GO 8 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 7 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 13 PDBsum 13 PIR 1 PIRSF 1 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 1 SMART 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 16172919

Title: Cloning, expression and characterization of the human NOB1 gene.

PubMed ID: 16172919

DOI: 10.1007/s11033-005-3141-7

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 1791827

Title: Cloning and expression of a new human polypeptide which regulates protein phosphorylation in Escherichia coli.

PubMed ID: 1791827

DOI: 10.1007/bf00225511

PubMed ID: 15231747

Title: A protein interaction framework for human mRNA degradation.

PubMed ID: 15231747

DOI: 10.1101/gr.2122004

PubMed ID: 17081983

Title: Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.

PubMed ID: 17081983

DOI: 10.1016/j.cell.2006.09.026

PubMed ID: 18691976

Title: Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.

PubMed ID: 18691976

DOI: 10.1016/j.molcel.2008.07.007

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19413330

Title: Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.

PubMed ID: 19413330

DOI: 10.1021/ac9004309

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21406692

Title: System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation.

PubMed ID: 21406692

DOI: 10.1126/scisignal.2001570

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 29875412

Title: Visualizing late states of human 40S ribosomal subunit maturation.

PubMed ID: 29875412

DOI: 10.1038/s41586-018-0193-0

Sequence Information:

  • Length: 412
  • Mass: 46675
  • Checksum: 369680A6DCFC7CB0
  • Sequence:
    • MAPVEHVVAD AGAFLRHAAL QDIGKNIYTI REVVTEIRDK ATRRRLAVLP YELRFKEPLP 
EYVRLVTEFS KKTGDYPSLS ATDIQVLALT YQLEAEFVGV SHLKQEPQKV KVSSSIQHPE 
TPLHISGFHL PYKPKPPQET EKGHSACEPE NLEFSSFMFW RNPLPNIDHE LQELLIDRGE 
DVPSEEEEEE ENGFEDRKDD SDDDGGGWIT PSNIKQIQQE LEQCDVPEDV RVGCLTTDFA 
MQNVLLQMGL HVLAVNGMLI REARSYILRC HGCFKTTSDM SRVFCSHCGN KTLKKVSVTV 
SDDGTLHMHF SRNPKVLNPR GLRYSLPTPK GGKYAINPHL TEDQRFPQLR LSQKARQKTN 
VFAPDYIAGV SPFVENDISS RSATLQVRDS TLGAGRRRLN PNASRKKFVK KR