Details for: TCP11

Gene ID: 6954

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: TCP11

Ensembl ID: ENSG00000124678

Description: t-complex 11

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • ependymal cell CL0000065
    CSI 8.2
    rCSI 16.64%
    PRS 99.93
  • neural progenitor cell CL0011020
    CSI 3.94
    rCSI 17.31%
    PRS 99.65

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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  • Node Size: Proportional to Target Cell CSI magnitude
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [TCP11](/details-gene/6954) (t-complex 11) is a protein-coding gene located on human chromosome 6p21.31. Functionally, it is strongly implicated in male reproduction, with established roles in germ cell development, spermatogenesis, and sperm function ([Link](https://doi.org/10.1620/tjem.224.111)). Gene Ontology annotations place the protein product in key sperm structures, including the acrosomal vesicle and flagellum, and link it to signaling pathways such as G protein-coupled receptor signaling. While literature emphasizes its testis-specific nature, expression data from an **Overall** context also indicates high significance in non-reproductive cell types, specifically [ependymal cell](/details-cell/CL0000065)s and [neural progenitor cell](/details-cell/CL0011020)s, suggesting a potentially broader, uncharacterized role. ## Cellular Roles and Expression Landscape The expression profile of [TCP11](/details-gene/6954) presents a dual landscape. **Overall**, analysis identifies its most significant expression in cells of the central nervous system, including [ependymal cell](/details-cell/CL0000065) (CSI: 8.20) and [neural progenitor cell](/details-cell/CL0011020) (CSI: 3.94). This suggests a potential function within the brain, possibly related to ciliary function in ependymal cells or aspects of neurogenesis. However, this finding contrasts with extensive literature and functional data that firmly establish [TCP11](/details-gene/6954) as a key gene in male reproduction ([Link](https://doi.org/10.1093/molehr/8.1.24)). Multiple studies characterize it as a testis-specific gene product crucial for normal sperm morphology and function ([Link](https://doi.org/10.1620/tjem.224.111)). This specialization is supported by its annotated localization to the [acrosomal vesicle](/details-cell/GO:0001669), [sperm flagellum](/details-cell/GO:0036126), and [sperm midpiece](/details-cell/GO:0097225). The discrepancy between the broad expression data and the focused functional annotation suggests that while its primary role is in spermatogenesis, it may have secondary or context-dependent functions in the nervous system. ## Pathways and Molecular Function The molecular functions of [TCP11](/details-gene/6954) are centered on male fertility and cell signaling. Its involvement in biological processes is well-defined, including [spermatogenesis](/details-cell/GO:0007283), [germ cell development](/details-cell/GO:0007281), and the [regulation of sperm capacitation](/details-cell/GO:1902490). These annotations are consistent with its identification as a potential determinant of sperm morphology and its role as the human homolog of the receptor for fertilization promoting peptide ([Link](https://doi.org/10.1093/molehr/8.1.24)). Mechanistically, [TCP11](/details-gene/6954) is associated with the [adenylate cyclase-activating g protein-coupled receptor signaling pathway](/details-cell/GO:0007189) and [protein kinase a signaling](/details-cell/GO:0010737). This suggests it may function as a receptor or a component of a signal transduction cascade on the sperm membrane, possibly mediating events required for fertilization. The gene product is known to be a membrane-associated protein ([GO:0016020](https://www.ebi.ac.uk/QuickGO/term/GO:0016020)) that participates in [protein binding](/details-cell/GO:0005515), further supporting a role in cell-cell communication or signal reception. ## Research Directions The available data highlights a compelling discrepancy between the established testis-specific role of [TCP11](/details-gene/6954) and its observed high significance in specific neural cell types. This points toward novel avenues of investigation. **Proposed Hypotheses:** 1. [TCP11](/details-gene/6954) possesses a previously uncharacterized function in the central nervous system, specifically in the development or maintenance of ciliated cells like [ependymal cell](/details-cell/CL0000065)s, which could be related to its role in forming the motile sperm flagellum. 2. The expression of [TCP11](/details-gene/6954) in [neural progenitor cell](/details-cell/CL0011020)s suggests it may be part of a conserved developmental program that is reactivated and specialized for spermatogenesis, with a more transient or subtle role during neurogenesis. 3. As a G protein-coupled receptor pathway component, [TCP11](/details-gene/6954) acts as a chemosensor on the sperm surface, essential for guiding the sperm towards the oocyte or for initiating the acrosome reaction in response to signals within the female reproductive tract. **Experimental Approach:** To test the first hypothesis regarding a neurological role, a robust experimental plan would be necessary. Initially, single-cell RNA sequencing and in situ hybridization on human and mouse brain tissue, particularly focusing on the lining of the cerebral ventricles, would be required to validate and map the precise expression of [TCP11](/details-gene/6954) within [ependymal cell](/details-cell/CL0000065)s and their progenitors. Following confirmation, a conditional knockout of *Tcp11* in mouse neural stem cells or ependymal cells could be generated. Phenotypic analysis of these mice would focus on ependymal layer integrity, cilia structure and beat frequency using electron microscopy and high-speed video microscopy, and potential defects in cerebrospinal fluid dynamics. **Therapeutic Potential:** Given its highly specific and critical role in spermatogenesis and sperm morphology ([Link](https://doi.org/10.1620/tjem.224.111)), [TCP11](/details-gene/6954) stands out as a potential diagnostic marker for specific forms of male infertility associated with OMIM phenotype [186982](https://omim.org/entry/186982). Mutations or expression-level changes in [TCP11](/details-gene/6954) could explain certain cases of asthenozoospermia (reduced sperm motility) or teratozoospermia (abnormal sperm morphology). While direct therapeutic intervention is challenging, understanding its signaling pathway could open doors to pharmacological strategies aimed at enhancing sperm function or capacitation in assisted reproductive technologies. It is not a conventional drug target for inhibition, but rather a component whose proper function is essential for fertility.

Genular Protein ID: 3572763530

Symbol: TCP11_HUMAN

Name: T-complex protein 11 homolog

UniProtKB Accession Codes:

Q8WWU5 B2RCE9 B3KQ27 B7Z7B5 B7Z7G1 B7Z7H4 B7Z7S8 E7EP29 J3KNG1 Q8NF85 Q9NQZ9 Q9NR39

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 5 CTD 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 18 Ensembl 9 ExpressionAtlas 1 GO 9 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 1 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 6 RNAct 1 RefSeq 7 STRING 1 SignaLink 1 TopDownProteomics 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 11756566

Title: Molecular characterization of the TCP11 gene which is the human homologue of the mouse gene encoding the receptor of fertilization promoting peptide.

PubMed ID: 11756566

DOI: 10.1093/molehr/8.1.24

PubMed ID: 12545228

Title: Cloning, expression and alternative splicing of a novel isoform of human TCP11b gene.

PubMed ID: 12545228

PubMed ID: 12905703

Title: Cloning, expression, and alternative splicing of the novel isoform of hTCP11 gene.

PubMed ID: 12905703

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 21597245

Title: Human t-complex protein 11 (TCP11), a testis-specific gene product, is a potential determinant of the sperm morphology.

PubMed ID: 21597245

DOI: 10.1620/tjem.224.111

Sequence Information:

  • Length: 503
  • Mass: 56141
  • Checksum: F567492970A3F68B
  • Sequence:
    • MPDVKESVPP KYPGDSEGRS CKPETSGPPQ EDKSGSEDPP PFLSVTGLTE TVNEVSKLSN 
KIGMNCDYYM EEKVLPPSSL EGKVKETVHN AFWDHLKEQL SATPPDFSCA LELLKEIKEI 
LLSLLLPRQN RLRIEIEEAL DMDLLKQEAE HGALKVLYLS KYVLNMMALL CAPVRDEAVQ 
KLENITDPVW LLRGIFQVLG RMKMDMVNYT IQSLQPHLQE HSIQYERAKF QELLNKQPSL 
LNHTTKWLTQ AAGDLTMSPP TCPDTSDSSS VAGPSPNEAA NNPEPLSPTM VLCQGFLNLL 
LWDLENEEFP ETLLMDRTRL QELKSQLHQL TVMASVLLVA SSFSGSVLFG SPQFVDKLKR 
ITKSLLEDFH SRPEEAILTV SEQVSQEIHQ SLKNMGLVAL SSDNTASLMG QLQNIAKKEN 
CVCSVIDQRI HLFLKCCLVL GVQRSLLDLP GGLTLIEAEL AELGQKFVNL THHNQQVFGP 
YYTEILKTLI SPAQALETKV ESV

Genular Protein ID: 1521343313

Symbol: B7ZAT8_HUMAN

Name: N/A

UniProtKB Accession Codes:

B7ZAT8

Database IDs:

ARBA 1 AlphaFoldDB 1 BioGRID-ORCS 1 CTD 1 DNASU 1 DisGeNET 1 EMBL 2 InterPro 1 KEGG 1 NCBI Taxonomy 1 OrthoDB 1 PANTHER 2 PeptideAtlas 1 Pfam 1 RefSeq 1 SAM 1

Sequence Information:

  • Length: 511
  • Mass: 56823
  • Checksum: 073D89349A2C68D9
  • Sequence:
    • MTRGGGGGDT ISKMPDVKES VPPKYPGDSE GRSCKPETSG PPQEDKSGSE DPPPCLTETV 
NEVSKLSNKI GMNCDYYMEE KVLPPSSLEG KVKETVHNAF WDHLKEQLSA TPPDFSCALE 
LLKEIKEILL SLLLPRQNRL RIEIEEALDM DLLKQEAEHG ALKVLYLSKY VLNMMALLCA 
PVRDEAVQKL ENITDPVWLL RGIFQVLGRM KMDMVNYTIQ SLQPHLQEHS IQYERAKFQE 
LLNKQPSLLN HTTKWLTQAA GDLTMSPPTC PDTSDSSSVA GPSPNEAANN PEPLSPTMVL 
CQGLLNLLLW DLENEEFPET LLMDRTRLQE LKSQLHQLTV MASVLLVASS FSGSVLFGSP 
QFVDKLKRIT KSLLEDFHSR PEEAILTVSE QVSQEIHQSL KNMGLVALSS DNTASLMGQL 
QNIAKKENCV CSVIDQRIHL FLKCCLVLGV QRSLLDLPGG LTLIEAELAE LGQKFVNLTH 
HNQQVFGPYY TEILKTLISP AQALETKVES V