Details for: ZNF205

Gene ID: 7755

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF205

Ensembl ID: ENSG00000122386

Description: zinc finger protein 205

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

  • Gene expression (transcription)
    (R-HSA-74160)
  • Generic transcription pathway
    (R-HSA-212436)
  • Rna polymerase ii transcription
    (R-HSA-73857)
  • Dna-binding transcription factor activity, rna polymerase ii-specific
    (GO:0000981)
  • Dna-binding transcription repressor activity, rna polymerase ii-specific
    (GO:0001227)
  • Mitochondrion
    (GO:0005739)
  • Negative regulation of transcription by rna polymerase ii
    (GO:0000122)
  • Nucleus
    (GO:0005634)
  • Positive regulation of hydrogen peroxide biosynthetic process
    (GO:0010729)
  • Positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway
    (GO:1901030)
  • Protein binding
    (GO:0005515)
  • Regulation of dna-templated transcription
    (GO:0006355)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)
  • Zinc ion binding
    (GO:0008270)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • progenitor cell CL0011026
    CSI 5.73
    rCSI 12.18%
    PRS 94.58
  • interneuron CL0000099
    CSI 4.81
    rCSI 9.65%
    PRS 95.69
  • mesodermal cell CL0000222
    CSI 3.45
    rCSI 4.14%
    PRS 98.1
  • neural crest cell CL0011012
    CSI 3.15
    rCSI 2.49%
    PRS 95.9
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 2.55
    rCSI 3.28%
    PRS 96.43
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 2.27
    rCSI 4.01%
    PRS 93.09
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.11
    rCSI 6.24%
    PRS 97.4
  • peripheral nervous system neuron CL2000032
    CSI 2.02
    rCSI 2.76%
    PRS 95.1

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF205](/details-gene/7755), or Zinc Finger Protein 205, is a protein-coding gene located on chromosome 16p13.3. It functions as a DNA-binding transcription factor, primarily involved in the negative regulation of transcription by RNA polymerase II. Functional annotations and expression data strongly suggest a role in developmental processes, particularly within the nervous system. **Overall**, it exhibits its highest significance in [progenitor cell](/details-cell/CL0011026)s and various types of [interneuron](/details-cell/CL0000099)s and their precursors, indicating a potential function in maintaining pluripotency and guiding neural cell fate. Beyond its nuclear role, it is also associated with mitochondria, where it may influence apoptotic signaling and cellular responses to oxidative stress ([Link](https://doi.org/10.1016/j.freeradbiomed.2012.01.003)). ## Cellular Roles and Expression Landscape The expression profile of [ZNF205](/details-gene/7755) highlights its specialized role in development and the nervous system. **Overall**, the gene shows the highest significance in undifferentiated or early-stage cell types, including [progenitor cell](/details-cell/CL0011026) (CSI: 5.73), [mesodermal cell](/details-cell/CL0000222) (CSI: 3.45), and [neural crest cell](/details-cell/CL0011012) (CSI: 3.15). This developmental association is further specified within the neuronal lineage, where [ZNF205](/details-gene/7755) is a significant marker for multiple neuronal subtypes and their precursors. High significance is observed in [interneuron](/details-cell/CL0000099) (CSI: 4.81), [neuroblast (sensu Vertebrata)](/details-cell/CL0000031) (CSI: 2.55), [caudal ganglionic eminence derived cortical interneuron](/details-cell/CL4023064) (CSI: 2.27), and [cerebral cortex GABAergic interneuron](/details-cell/CL0010011) (CSI: 2.11). This pattern suggests that [ZNF205](/details-gene/7755) may be integral to the transcriptional programs that establish and maintain the identity of specific neuronal populations, particularly those of the central and peripheral nervous systems. ## Pathways and Molecular Function Functionally, [ZNF205](/details-gene/7755) is primarily characterized as a nuclear protein that modulates gene expression. Gene Ontology (GO) annotations confirm its role in [regulation of dna-templated transcription](/details-cell/GO:0006355) and specifically as a [dna-binding transcription repressor activity, rna polymerase ii-specific](/details-cell/GO:0001227). Its molecular activity is dependent on [zinc ion binding](/details-cell/GO:0008270), consistent with its identity as a zinc finger protein. Reactome pathway analysis further places it within the core transcriptional machinery, including [Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and [Rna polymerase ii transcription](https://reactome.org/content/detail/R-HSA-73857). Interestingly, [ZNF205](/details-gene/7755) also has functions outside the nucleus. It is annotated to the [mitochondrion](/details-cell/GO:0005739) and is implicated in the [positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway](/details-cell/GO:1901030). Research has identified it as a transcriptional repressor that can mitigate mitochondrial dysfunction, potentially linking nuclear gene regulation with cellular metabolic and survival pathways ([Link](https://doi.org/10.1016/j.freeradbiomed.2012.01.003)). It is also involved in the [positive regulation of hydrogen peroxide biosynthetic process](/details-cell/GO:0010729), suggesting a role in redox homeostasis. ## Research Directions The specific expression of [ZNF205](/details-gene/7755) in progenitor and early neuronal cells, combined with its function as a transcriptional repressor, points toward a critical role in neurodevelopment. The dual localization in the nucleus and mitochondria suggests a mechanism for coordinating transcriptional programs with metabolic state, a crucial aspect of cell fate decisions. ### Proposed Hypotheses: 1. **[ZNF205](/details-gene/7755) functions as a key repressor of differentiation genes in neural progenitor cells, thereby helping to maintain their self-renewal capacity.** Its downregulation would be a prerequisite for terminal differentiation into mature neuronal subtypes. 2. **During neuronal development, [ZNF205](/details-gene/7755) acts as a molecular link between the nucleus and mitochondria, repressing specific metabolic genes to protect neuroblasts from oxidative stress-induced apoptosis.** This would ensure the survival of the correct number of neurons during corticogenesis. ### Experimental Approach: To test the first hypothesis, a compelling experiment would involve the use of human induced pluripotent stem cell (iPSC)-derived neural progenitor cells (NPCs). - **Methodology:** A CRISPR interference (CRISPRi) system could be employed to achieve a stable and titratable knockdown of [ZNF205](/details-gene/7755) expression in NPCs. Control and knockdown cells would then be subjected to a standard neuronal differentiation protocol. - **Analysis:** Over a time course, cell populations would be analyzed using single-cell RNA sequencing (scRNA-seq) to map differentiation trajectories and identify prematurely expressed genes in the knockdown group. This would be complemented by immunocytochemistry for markers of pluripotency (e.g., SOX2) and neuronal differentiation (e.g., DCX, MAP2) to quantify changes in cell fate commitment. ### Therapeutic Potential: As a DNA-binding transcription factor, [ZNF205](/details-gene/7755) represents a challenging therapeutic target for traditional small molecule inhibitors. However, its specific expression pattern in progenitor cells could make it relevant in pathological contexts such as neuroblastoma or other pediatric cancers that often hijack developmental pathways. If [ZNF205](/details-gene/7755) is found to repress tumor suppressor genes, developing strategies to inhibit its function (e.g., via targeted protein degradation) could be a viable therapeutic avenue. Conversely, if it represses oncogenic drivers, enhancing its activity or expression could be beneficial. Its high specificity for developmental lineages suggests that targeting [ZNF205](/details-gene/7755) might offer a therapeutic window with limited toxicity to differentiated, healthy tissues.

Genular Protein ID: 2119571685

Symbol: RHIT_HUMAN

Name: Zinc finger protein 205

UniProtKB Accession Codes:

O95201 A8MZK0 D3DUB4 I0J0A3 Q9BU95

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 8 Ensembl 3 ExpressionAtlas 1 GO 11 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 Reactome 1 RefSeq 4 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9787081

Title: Identification of two Kruppel-related zinc finger genes (ZNF200 and ZNF210) from human chromosome 16p13.3.

PubMed ID: 9787081

DOI: 10.1006/geno.1998.5430

PubMed ID: 22306510

Title: Identification of Rhit as a novel transcriptional repressor of human Mpv17-like protein with a mitigating effect on mitochondrial dysfunction, and its transcriptional regulation by FOXD3 and GABP.

PubMed ID: 22306510

DOI: 10.1016/j.freeradbiomed.2012.01.003

PubMed ID: 15616553

Title: The sequence and analysis of duplication-rich human chromosome 16.

PubMed ID: 15616553

DOI: 10.1038/nature03187

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 25772364

Title: SUMO-2 orchestrates chromatin modifiers in response to DNA damage.

PubMed ID: 25772364

DOI: 10.1016/j.celrep.2015.02.033

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

Sequence Information:

  • Length: 554
  • Mass: 60630
  • Checksum: B70DC15F0DFDC7FC
  • Sequence:
    • MSADGGGIQD TQDKETPPEV PDRGHPHQEM PSKLGEAVPS GDTQESLHIK MEPEEPHSEG 
ASQEDGAQGA WGWAPLSHGS KEKALFLPGG ALPSPRIPVL SREGRTRDRQ MAAALLTAWS 
QMPVTFEDVA LYLSREEWGR LDHTQQNFYR DVLQKKNGLS LGFPFSRPFW APQAHGKGEA 
SGSSRQAGDE KEWRGACTGA VEVGQRVQTS SVAALGNVKP FRTRAGRVQW GVPQCAQEAA 
CGRSSGPAKD SGQPAEPDRT PDAAPPDPSP TEPQEYRVPE KPNEEEKGAP ESGEEGLAPD 
SEVGRKSYRC EQCGKGFSWH SHLVTHRRTH TGEKPYACTD CGKRFGRSSH LIQHQIIHTG 
EKPYTCPACR KSFSHHSTLI QHQRIHTGEK PYVCDRCAKR FTRRSDLVTH QGTHTGAKPH 
KCPICAKCFT QSSALVTHQR THTGVKPYPC PECGKCFSQR SNLIAHNRTH TGEKPYHCLD 
CGKSFSHSSH LTAHQRTHRG VRPYACPLCG KSFSRRSNLH RHEKIHTTGP KALAMLMLGA 
AAAGALATPP PAPT