Details for: SPOP

Gene ID: 8405

Symbol: SPOP

Ensembl ID: ENSG00000121067

Description: speckle type BTB/POZ protein

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 333.2471
    Cell Significance Index: -51.8400
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 195.4713
    Cell Significance Index: -49.5800
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 121.8327
    Cell Significance Index: -57.5200
  • Cell Name: embryonic stem cell (CL0002322)
    Fold Change: 119.9910
    Cell Significance Index: -49.4300
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 119.9487
    Cell Significance Index: -48.7300
  • Cell Name: peripheral blood mononuclear cell (CL2000001)
    Fold Change: 108.0319
    Cell Significance Index: -55.5700
  • Cell Name: ileal goblet cell (CL1000326)
    Fold Change: 85.8839
    Cell Significance Index: -57.6300
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 51.5322
    Cell Significance Index: -49.2000
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 44.8394
    Cell Significance Index: -55.2900
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 19.0510
    Cell Significance Index: -51.0400
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 16.0380
    Cell Significance Index: -49.2600
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 14.3865
    Cell Significance Index: -56.7700
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 13.6892
    Cell Significance Index: -29.9600
  • Cell Name: epithelial cell of pancreas (CL0000083)
    Fold Change: 11.9525
    Cell Significance Index: 196.9600
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 2.1344
    Cell Significance Index: 147.6100
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 1.6919
    Cell Significance Index: 339.3900
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 1.5694
    Cell Significance Index: 120.4300
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 1.5495
    Cell Significance Index: 307.5000
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 1.1467
    Cell Significance Index: 60.2100
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: 1.1096
    Cell Significance Index: 57.8000
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 1.0416
    Cell Significance Index: 373.6000
  • Cell Name: skeletal muscle fiber (CL0008002)
    Fold Change: 1.0333
    Cell Significance Index: 26.5600
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 1.0262
    Cell Significance Index: 111.6200
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.7794
    Cell Significance Index: 539.0500
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 0.7742
    Cell Significance Index: 106.3300
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.7236
    Cell Significance Index: 653.3900
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.5705
    Cell Significance Index: 102.8400
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.5634
    Cell Significance Index: 69.2800
  • Cell Name: odontoblast (CL0000060)
    Fold Change: 0.5290
    Cell Significance Index: 67.8200
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.5097
    Cell Significance Index: 278.3600
  • Cell Name: preadipocyte (CL0002334)
    Fold Change: 0.4919
    Cell Significance Index: 9.6000
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.4664
    Cell Significance Index: 206.2000
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.4586
    Cell Significance Index: 45.3700
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 0.3921
    Cell Significance Index: 22.0000
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: 0.3663
    Cell Significance Index: 12.7300
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.3620
    Cell Significance Index: 16.8800
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: 0.3505
    Cell Significance Index: 23.5700
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.2917
    Cell Significance Index: 6.3200
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.2633
    Cell Significance Index: 7.5900
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 0.2612
    Cell Significance Index: 118.5700
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.2589
    Cell Significance Index: 16.3200
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 0.2575
    Cell Significance Index: 16.6100
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: 0.2068
    Cell Significance Index: 12.7100
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: 0.1687
    Cell Significance Index: 7.4600
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.1483
    Cell Significance Index: 24.1200
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.1449
    Cell Significance Index: 17.0900
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: 0.1432
    Cell Significance Index: 90.9500
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: 0.1299
    Cell Significance Index: 4.9200
  • Cell Name: cerebellar granule cell (CL0001031)
    Fold Change: 0.1240
    Cell Significance Index: 2.1300
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.1067
    Cell Significance Index: 20.3100
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: 0.0827
    Cell Significance Index: 155.6800
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.0525
    Cell Significance Index: 8.9600
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.0351
    Cell Significance Index: 0.9800
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 0.0349
    Cell Significance Index: 2.4700
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: -0.0062
    Cell Significance Index: -0.2900
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0109
    Cell Significance Index: -16.8000
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0125
    Cell Significance Index: -23.0600
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0135
    Cell Significance Index: -18.3800
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0172
    Cell Significance Index: -10.7600
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: -0.0215
    Cell Significance Index: -0.3600
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: -0.0228
    Cell Significance Index: -17.2300
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0312
    Cell Significance Index: -23.0900
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0355
    Cell Significance Index: -26.0000
  • Cell Name: cardiac muscle cell (CL0000746)
    Fold Change: -0.0616
    Cell Significance Index: -0.9100
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0670
    Cell Significance Index: -37.8000
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.1029
    Cell Significance Index: -10.5100
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.1361
    Cell Significance Index: -14.1800
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.1518
    Cell Significance Index: -31.9700
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.1747
    Cell Significance Index: -50.2600
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.1906
    Cell Significance Index: -22.2200
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: -0.2030
    Cell Significance Index: -4.2500
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.2031
    Cell Significance Index: -29.5300
  • Cell Name: lactocyte (CL0002325)
    Fold Change: -0.2033
    Cell Significance Index: -26.2700
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: -0.2187
    Cell Significance Index: -11.3600
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.2646
    Cell Significance Index: -30.3200
  • Cell Name: leptomeningeal cell (CL0000708)
    Fold Change: -0.2737
    Cell Significance Index: -5.8500
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.2788
    Cell Significance Index: -7.3300
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.2947
    Cell Significance Index: -9.4400
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: -0.3202
    Cell Significance Index: -8.5500
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: -0.3399
    Cell Significance Index: -25.3300
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: -0.3649
    Cell Significance Index: -16.5400
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: -0.3762
    Cell Significance Index: -9.6100
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: -0.3841
    Cell Significance Index: -8.1800
  • Cell Name: pancreatic endocrine cell (CL0008024)
    Fold Change: -0.3872
    Cell Significance Index: -44.2000
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: -0.4728
    Cell Significance Index: -12.8700
  • Cell Name: GABAergic amacrine cell (CL4030027)
    Fold Change: -0.4729
    Cell Significance Index: -5.8700
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: -0.5689
    Cell Significance Index: -14.2200
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.5953
    Cell Significance Index: -47.1500
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: -0.6129
    Cell Significance Index: -21.5400
  • Cell Name: hippocampal pyramidal neuron (CL1001571)
    Fold Change: -0.6330
    Cell Significance Index: -18.0700
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: -0.7091
    Cell Significance Index: -19.0000
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: -0.7118
    Cell Significance Index: -20.4100
  • Cell Name: cortical interneuron (CL0008031)
    Fold Change: -0.7647
    Cell Significance Index: -18.3400
  • Cell Name: peg cell (CL4033014)
    Fold Change: -0.7687
    Cell Significance Index: -17.7600
  • Cell Name: type I muscle cell (CL0002211)
    Fold Change: -0.7906
    Cell Significance Index: -19.2900
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.9283
    Cell Significance Index: -56.9200
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: -0.9619
    Cell Significance Index: -28.2500
  • Cell Name: retinal rod cell (CL0000604)
    Fold Change: -0.9723
    Cell Significance Index: -11.5900
  • Cell Name: fibro/adipogenic progenitor cell (CL0009099)
    Fold Change: -0.9907
    Cell Significance Index: -50.0700
  • Cell Name: centrilobular region hepatocyte (CL0019029)
    Fold Change: -1.0056
    Cell Significance Index: -16.9400

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** SPOP is a 74-kDa protein with two BTB domains, two POZ domains, and a speckle-type domain. The BTB domain is a plant homeostasis 10 (PHO10) domain, while the POZ domain is a P-box domain. The speckle-type domain is characterized by a unique structure that mediates interactions with other proteins. SPOP is predominantly expressed in cells of the immune system, including ciliary muscle cells, smooth muscle cells of the sphincter of the pupil, astrocytes of the cerebral cortex, and smooth muscle cells of the prostate. **Pathways and Functions:** SPOP is involved in various signaling pathways, including: 1. **Cul3-Ring Ubiquitin Ligase Complex:** SPOP interacts with the Cul3-Ring Ubiquitin Ligase Complex, a key component of the ubiquitin-proteasome pathway, to regulate protein degradation and immune responses. 2. **Hedgehog Signaling:** SPOP acts as a negative regulator of Hedgehog signaling, a critical pathway involved in development, growth, and immune responses. 3. **Proteasome-Mediated Ubiquitin-Dependent Protein Catabolic Process:** SPOP is involved in the regulation of protein degradation through the proteasome pathway, influencing immune responses and cellular homeostasis. 4. **Signal Transduction:** SPOP interacts with various signaling molecules, including proteins of the Cul3-Ring Ubiquitin Ligase Complex, to regulate signal transduction pathways. **Clinical Significance:** Dysregulation of SPOP has been implicated in various immune-related disorders, including: 1. **Multiple Myeloma:** SPOP mutations have been identified in multiple myeloma, a type of blood cancer characterized by the proliferation of malignant plasma cells. 2. **Leukemia:** SPOP alterations have been found in certain types of leukemia, including acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML). 3. **Autoimmune Diseases:** SPOP dysregulation has been implicated in autoimmune diseases, such as rheumatoid arthritis and lupus, where immune system dysregulation leads to tissue damage and inflammation. In conclusion, SPOP is a multifunctional protein with a complex role in immune regulation, protein degradation, and signaling pathways. Its dysregulation has been implicated in various immune-related disorders, highlighting the importance of SPOP in maintaining immune homeostasis and preventing disease. Further research is needed to fully elucidate the functions and clinical significance of SPOP in human disease.

Genular Protein ID: 2502911978

Symbol: SPOP_HUMAN

Name: HIB homolog 1

UniProtKB Accession Codes:

O43791 B2R6S3 D3DTW7 Q53HJ1

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 3 CORUM 1 CTD 1 ChEMBL 1 ChiTaRS 1 ComplexPortal 2 DIP 1 DNASU 1 DisGeNET 1 ELM 1 EMBL 10 EMDB 5 Ensembl 7 EvolutionaryTrace 1 ExpressionAtlas 1 GO 11 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 IDEAL 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 5 MINT 1 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 2 OrthoDB 1 PANTHER 2 PDB 31 PDBsum 31 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 9 SIGNOR 1 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 UniPathway 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9414087

Title: Identification of a novel nuclear speckle-type protein, SPOP.

PubMed ID: 9414087

DOI: 10.1016/s0014-5793(97)01340-9

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 14528312

Title: Targeting of protein ubiquitination by BTB-Cullin 3-Roc1 ubiquitin ligases.

PubMed ID: 14528312

DOI: 10.1038/ncb1056

PubMed ID: 15240113

Title: Daxx-mediated transcriptional repression of MMP1 gene is reversed by SPOP.

PubMed ID: 15240113

DOI: 10.1016/j.bbrc.2004.06.022

PubMed ID: 15897469

Title: Stable X chromosome inactivation involves the PRC1 Polycomb complex and requires histone MACROH2A1 and the CULLIN3/SPOP ubiquitin E3 ligase.

PubMed ID: 15897469

DOI: 10.1073/pnas.0408918102

PubMed ID: 16651542

Title: Roadkill attenuates Hedgehog responses through degradation of Cubitus interruptus.

PubMed ID: 16651542

DOI: 10.1242/dev.02370

PubMed ID: 16740475

Title: A hedgehog-induced BTB protein modulates hedgehog signaling by degrading Ci/Gli transcription factor.

PubMed ID: 16740475

DOI: 10.1016/j.devcel.2006.05.004

PubMed ID: 16524876

Title: BTB domain-containing speckle-type POZ protein (SPOP) serves as an adaptor of Daxx for ubiquitination by Cul3-based ubiquitin ligase.

PubMed ID: 16524876

DOI: 10.1074/jbc.m600204200

PubMed ID: 22085717

Title: Breast cancer metastasis suppressor 1 (BRMS1) is destabilized by the Cul3-SPOP E3 ubiquitin ligase complex.

PubMed ID: 22085717

DOI: 10.1016/j.bbrc.2011.10.154

PubMed ID: 36791496

Title: SPOP is essential for DNA replication licensing through maintaining translation of CDT1 and CDC6 in HaCaT cells.

PubMed ID: 36791496

DOI: 10.1016/j.bbrc.2023.02.012

PubMed ID: 37622993

Title: SPOP targets the immune transcription factor IRF1 for proteasomal degradation.

PubMed ID: 37622993

DOI: 10.7554/elife.89951

PubMed ID: 37796126

Title: Ubiquitin E3 ligase SPOP is a host negative regulator of enterovirus 71-encoded 2A protease.

PubMed ID: 37796126

DOI: 10.1128/jvi.00786-23

PubMed ID: 38018242

Title: SPOP inhibits HBV transcription and replication by ubiquitination and degradation of HNF1alpha.

PubMed ID: 38018242

DOI: 10.1002/jmv.29254

PubMed ID: 19818708

Title: Structures of SPOP-substrate complexes: insights into molecular architectures of BTB-Cul3 ubiquitin ligases.

PubMed ID: 19818708

DOI: 10.1016/j.molcel.2009.09.022

PubMed ID: 22632832

Title: Adaptor protein self-assembly drives the control of a cullin-RING ubiquitin ligase.

PubMed ID: 22632832

DOI: 10.1016/j.str.2012.04.009

PubMed ID: 32109420

Title: De Novo Variants in SPOP Cause Two Clinically Distinct Neurodevelopmental Disorders.

PubMed ID: 32109420

DOI: 10.1016/j.ajhg.2020.02.001

Sequence Information:

  • Length: 374
  • Mass: 42132
  • Checksum: EE5F4C5CF6FD09DC
  • Sequence:
    • MSRVPSPPPP AEMSSGPVAE SWCYTQIKVV KFSYMWTINN FSFCREEMGE VIKSSTFSSG 
ANDKLKWCLR VNPKGLDEES KDYLSLYLLL VSCPKSEVRA KFKFSILNAK GEETKAMESQ 
RAYRFVQGKD WGFKKFIRRD FLLDEANGLL PDDKLTLFCE VSVVQDSVNI SGQNTMNMVK 
VPECRLADEL GGLWENSRFT DCCLCVAGQE FQAHKAILAA RSPVFSAMFE HEMEESKKNR 
VEINDVEPEV FKEMMCFIYT GKAPNLDKMA DDLLAAADKY ALERLKVMCE DALCSNLSVE 
NAAEILILAD LHSADQLKTQ AVDFINYHAS DVLETSGWKS MVVSHPHLVA EAYRSLASAQ 
CPFLGPPRKR LKQS

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.