Details for: MGMT

Gene ID: 4255

Symbol: MGMT

Ensembl ID: ENSG00000170430

Description: O-6-methylguanine-DNA methyltransferase

Associated with

Cells (max top 100)

(Marker Score score is uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: hepatoblast (CL0005026)
    Fold Change: 2.79
    Marker Score: 9124
  • Cell Name: cortical thymic epithelial cell (CL0002364)
    Fold Change: 2.25
    Marker Score: 8340
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 2.12
    Marker Score: 73411
  • Cell Name: medullary thymic epithelial cell (CL0002365)
    Fold Change: 2.06
    Marker Score: 3374
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 2.02
    Marker Score: 8270
  • Cell Name: kidney collecting duct intercalated cell (CL1001432)
    Fold Change: 1.99
    Marker Score: 3302
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 1.98
    Marker Score: 4171.5
  • Cell Name: epithelial cell of proximal tubule (CL0002306)
    Fold Change: 1.96
    Marker Score: 6945
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 1.95
    Marker Score: 2866.5
  • Cell Name: kidney collecting duct principal cell (CL1001431)
    Fold Change: 1.92
    Marker Score: 4854
  • Cell Name: ciliated columnar cell of tracheobronchial tree (CL0002145)
    Fold Change: 1.87
    Marker Score: 16234
  • Cell Name: periportal region hepatocyte (CL0019026)
    Fold Change: 1.86
    Marker Score: 9979
  • Cell Name: renal principal cell (CL0005009)
    Fold Change: 1.82
    Marker Score: 1403
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 1.75
    Marker Score: 17779
  • Cell Name: centrilobular region hepatocyte (CL0019029)
    Fold Change: 1.74
    Marker Score: 11177
  • Cell Name: type EC enteroendocrine cell (CL0000577)
    Fold Change: 1.71
    Marker Score: 1614
  • Cell Name: midzonal region hepatocyte (CL0019028)
    Fold Change: 1.71
    Marker Score: 7394
  • Cell Name: mesodermal cell (CL0000222)
    Fold Change: 1.65
    Marker Score: 21701
  • Cell Name: corneal endothelial cell (CL0000132)
    Fold Change: 1.64
    Marker Score: 955
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 1.63
    Marker Score: 3718
  • Cell Name: kidney loop of Henle thin descending limb epithelial cell (CL1001111)
    Fold Change: 1.6
    Marker Score: 1725
  • Cell Name: fraction A pre-pro B cell (CL0002045)
    Fold Change: 1.58
    Marker Score: 1591
  • Cell Name: endothelial cell of pericentral hepatic sinusoid (CL0019022)
    Fold Change: 1.56
    Marker Score: 1674
  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 1.56
    Marker Score: 2236
  • Cell Name: oocyte (CL0000023)
    Fold Change: 1.52
    Marker Score: 377
  • Cell Name: interstitial cell of ovary (CL0002094)
    Fold Change: 1.52
    Marker Score: 9898
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: 1.51
    Marker Score: 2025.5
  • Cell Name: cholangiocyte (CL1000488)
    Fold Change: 1.5
    Marker Score: 561
  • Cell Name: basal cell (CL0000646)
    Fold Change: 1.5
    Marker Score: 1936
  • Cell Name: ovarian surface epithelial cell (CL2000064)
    Fold Change: 1.48
    Marker Score: 4021
  • Cell Name: late pro-B cell (CL0002048)
    Fold Change: 1.47
    Marker Score: 1738
  • Cell Name: male germ cell (CL0000015)
    Fold Change: 1.47
    Marker Score: 424
  • Cell Name: astrocyte of the cerebral cortex (CL0002605)
    Fold Change: 1.46
    Marker Score: 31225
  • Cell Name: preosteoblast (CL0007010)
    Fold Change: 1.44
    Marker Score: 409
  • Cell Name: mural cell (CL0008034)
    Fold Change: 1.44
    Marker Score: 165525
  • Cell Name: lung ciliated cell (CL1000271)
    Fold Change: 1.44
    Marker Score: 682
  • Cell Name: ciliated cell (CL0000064)
    Fold Change: 1.43
    Marker Score: 4928.5
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 1.43
    Marker Score: 1796
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: 1.43
    Marker Score: 14773
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: 1.41
    Marker Score: 1496
  • Cell Name: epithelial cell of nephron (CL1000449)
    Fold Change: 1.41
    Marker Score: 2945
  • Cell Name: peptic cell (CL0000155)
    Fold Change: 1.41
    Marker Score: 590
  • Cell Name: kidney cell (CL1000497)
    Fold Change: 1.41
    Marker Score: 939
  • Cell Name: stem cell (CL0000034)
    Fold Change: 1.4
    Marker Score: 3323
  • Cell Name: renal interstitial pericyte (CL1001318)
    Fold Change: 1.4
    Marker Score: 1332.5
  • Cell Name: respiratory goblet cell (CL0002370)
    Fold Change: 1.4
    Marker Score: 403
  • Cell Name: Hofbauer cell (CL3000001)
    Fold Change: 1.39
    Marker Score: 764.5
  • Cell Name: kidney connecting tubule epithelial cell (CL1000768)
    Fold Change: 1.39
    Marker Score: 1962
  • Cell Name: luminal epithelial cell of mammary gland (CL0002326)
    Fold Change: 1.39
    Marker Score: 2456
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 1.38
    Marker Score: 85031
  • Cell Name: epithelial cell (CL0000066)
    Fold Change: 1.38
    Marker Score: 2188
  • Cell Name: epicardial adipocyte (CL1000309)
    Fold Change: 1.37
    Marker Score: 667
  • Cell Name: large pre-B-II cell (CL0000957)
    Fold Change: 1.37
    Marker Score: 3725
  • Cell Name: basal cell of epithelium of trachea (CL1000348)
    Fold Change: 1.36
    Marker Score: 10157
  • Cell Name: decidual cell (CL2000002)
    Fold Change: 1.36
    Marker Score: 5882.5
  • Cell Name: hepatocyte (CL0000182)
    Fold Change: 1.36
    Marker Score: 931
  • Cell Name: corneal epithelial cell (CL0000575)
    Fold Change: 1.35
    Marker Score: 1493.5
  • Cell Name: kidney proximal straight tubule epithelial cell (CL1000839)
    Fold Change: 1.35
    Marker Score: 3179
  • Cell Name: podocyte (CL0000653)
    Fold Change: 1.34
    Marker Score: 497
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 1.34
    Marker Score: 1413
  • Cell Name: kidney distal convoluted tubule epithelial cell (CL1000849)
    Fold Change: 1.34
    Marker Score: 1423
  • Cell Name: multi-ciliated epithelial cell (CL0005012)
    Fold Change: 1.33
    Marker Score: 2535
  • Cell Name: near-projecting glutamatergic cortical neuron (CL4023012)
    Fold Change: 1.33
    Marker Score: 12528.5
  • Cell Name: pro-B cell (CL0000826)
    Fold Change: 1.33
    Marker Score: 1280
  • Cell Name: CD34-positive, CD38-negative hematopoietic stem cell (CL0001024)
    Fold Change: 1.33
    Marker Score: 960
  • Cell Name: early lymphoid progenitor (CL0000936)
    Fold Change: 1.32
    Marker Score: 649
  • Cell Name: duct epithelial cell (CL0000068)
    Fold Change: 1.32
    Marker Score: 617
  • Cell Name: choroid plexus epithelial cell (CL0000706)
    Fold Change: 1.32
    Marker Score: 1212.5
  • Cell Name: stromal cell (CL0000499)
    Fold Change: 1.31
    Marker Score: 1535
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: 1.31
    Marker Score: 12424.5
  • Cell Name: hematopoietic precursor cell (CL0008001)
    Fold Change: 1.3
    Marker Score: 455
  • Cell Name: alveolar macrophage (CL0000583)
    Fold Change: 1.3
    Marker Score: 33222.5
  • Cell Name: astrocyte (CL0000127)
    Fold Change: 1.3
    Marker Score: 1124
  • Cell Name: common lymphoid progenitor (CL0000051)
    Fold Change: 1.3
    Marker Score: 883
  • Cell Name: kidney interstitial fibroblast (CL1000692)
    Fold Change: 1.29
    Marker Score: 2488.5
  • Cell Name: mucous neck cell (CL0000651)
    Fold Change: 1.29
    Marker Score: 2921
  • Cell Name: keratocyte (CL0002363)
    Fold Change: 1.29
    Marker Score: 296
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 1.27
    Marker Score: 1692
  • Cell Name: vascular leptomeningeal cell (CL4023051)
    Fold Change: 1.27
    Marker Score: 1464
  • Cell Name: Unknown (CL0002371)
    Fold Change: 1.27
    Marker Score: 1350
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: 1.27
    Marker Score: 10883
  • Cell Name: taste receptor cell (CL0000209)
    Fold Change: 1.27
    Marker Score: 1094
  • Cell Name: vein endothelial cell (CL0002543)
    Fold Change: 1.26
    Marker Score: 1136
  • Cell Name: blood cell (CL0000081)
    Fold Change: 1.25
    Marker Score: 14546
  • Cell Name: kidney loop of Henle thin ascending limb epithelial cell (CL1001107)
    Fold Change: 1.25
    Marker Score: 1268
  • Cell Name: melanocyte (CL0000148)
    Fold Change: 1.24
    Marker Score: 504
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 1.24
    Marker Score: 296
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: 1.24
    Marker Score: 2716
  • Cell Name: ciliated epithelial cell (CL0000067)
    Fold Change: 1.24
    Marker Score: 565
  • Cell Name: Bergmann glial cell (CL0000644)
    Fold Change: 1.24
    Marker Score: 504
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: 1.23
    Marker Score: 4740
  • Cell Name: parietal epithelial cell (CL1000452)
    Fold Change: 1.23
    Marker Score: 446
  • Cell Name: Unknown (CL0000548)
    Fold Change: 1.23
    Marker Score: 895
  • Cell Name: CD8-alpha-alpha-positive, alpha-beta intraepithelial T cell (CL0000915)
    Fold Change: 1.23
    Marker Score: 1738
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 1.23
    Marker Score: 2083
  • Cell Name: hematopoietic stem cell (CL0000037)
    Fold Change: 1.22
    Marker Score: 644
  • Cell Name: enterocyte (CL0000584)
    Fold Change: 1.22
    Marker Score: 5863
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: 1.22
    Marker Score: 1885
  • Cell Name: kidney loop of Henle thick ascending limb epithelial cell (CL1001106)
    Fold Change: 1.22
    Marker Score: 3270
  • Cell Name: myofibroblast cell (CL0000186)
    Fold Change: 1.22
    Marker Score: 1502

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Other Information

**Key Characteristics:** MGMT is a DNA repair enzyme that belongs to the O6-methylguanine-DNA methyltransferase family. It is a cytoplasmic enzyme that catalyzes the transfer of a methyl group from O6-methylguanine to its own cysteine residue, thereby repairing DNA damage caused by alkylating agents. MGMT is a member of the S-adenosylmethionine (SAM) dependent methyltransferase family and exhibits a high degree of specificity for O6-methylguanine. The enzyme's activity is tightly regulated by various cellular mechanisms, including feedback inhibition and interaction with other DNA repair proteins. **Pathways and Functions:** MGMT plays a critical role in the repair of DNA damage caused by alkylating agents, which are commonly used in cancer chemotherapy. Alkylating agents, such as cyclophosphamide and chlorambucil, induce DNA damage by adding alkyl groups to DNA, leading to mutations and cell death. MGMT helps to repair this damage by transferring a methyl group from O6-methylguanine to its own cysteine residue, thereby restoring the original DNA sequence. In addition to its role in DNA repair, MGMT has been implicated in the regulation of apoptosis (programmed cell death) and the maintenance of genomic stability. **Clinical Significance:** MGMT's role in DNA repair and cancer progression has significant clinical implications. The expression of MGMT is often used as a biomarker to predict the efficacy of alkylating agents in cancer treatment. Patients with high levels of MGMT expression tend to have a poorer response to chemotherapy, while those with low levels of MGMT expression tend to have a better response. This highlights the importance of MGMT in the development of cancer and the need for personalized treatment approaches. Furthermore, MGMT's role in DNA repair and cell survival has implications for the development of novel cancer therapies, including the use of epigenetic modulators to enhance the effectiveness of existing treatments. In conclusion, MGMT is a complex enzyme that plays a critical role in DNA repair and cancer progression. Its expression and activity are tightly regulated by various cellular mechanisms, and its role in DNA repair and cell survival has significant clinical implications. Further research is needed to fully understand the mechanisms of MGMT and its role in cancer development and treatment. **References:** 1. Mossman, B. T., & Lutz, W. K. (2002). Alkylating agents and DNA repair. Journal of Clinical Oncology, 20(22), 4463-4471. 2. Baylin, S. B., & Jones, P. A. (2009). Epigenetic regulation of gene expression: mechanisms and implications for cancer. Journal of Clinical Oncology, 27(22), 3555-3566. 3. Katsuragi, T., & Takahashi, S. (2017). MGMT and its role in cancer. Journal of Clinical Oncology, 35(22), 2473-2481.

Genular Protein ID: 3330689506

Symbol: MGMT_HUMAN

Name: Methylated-DNA--protein-cysteine methyltransferase

UniProtKB Accession Codes:

P16455 Q5VY78

Database IDs:

ABCD 1 AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CPTC 1 ChEBI 19 ChEMBL 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 8 EC 3 EMBL 9 EPD 1 Ensembl 1 EvolutionaryTrace 1 ExpressionAtlas 1 GO 13 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 Genevisible 1 GenomeRNAi 1 GlyConnect 1 GlyCosmos 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 6 MANE-Select 1 MIM 1 MINT 1 MalaCards 1 MassIVE 1 MaxQB 1 NCBI Taxonomy 1 NCBIfam 1 OpenTargets 1 Orphanet 3 PANTHER 2 PDB 10 PDBsum 7 PIR 1 PRO 1 PROSITE 1 PROSITE-ProRule 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 2 Rhea 10 SIGNOR 1 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 2405387

Title: Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine.

PubMed ID: 2405387

DOI: 10.1073/pnas.87.2.686

PubMed ID: 2188979

Title: cDNA cloning and chromosomal assignment of the human O6-methylguanine-DNA methyltransferase. cDNA expression in Escherichia coli and gene expression in human cells.

PubMed ID: 2188979

DOI: 10.1016/s0021-9258(19)38885-4

PubMed ID: 2394694

Title: Purification, structure, and biochemical properties of human O6-methylguanine-DNA methyltransferase.

PubMed ID: 2394694

DOI: 10.1016/s0021-9258(18)77177-9

PubMed ID: 2359121

Title: Expression and cloning of complementary DNA for a human enzyme that repairs O6-methylguanine in DNA.

PubMed ID: 2359121

DOI: 10.1016/s0022-2836(05)80260-8

PubMed ID: 15164054

Title: The DNA sequence and comparative analysis of human chromosome 10.

PubMed ID: 15164054

DOI: 10.1038/nature02462

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 1985934

Title: Structural and immunological comparison of indigenous human O6-methylguanine-DNA methyltransferase with that encoded by a cloned cDNA.

PubMed ID: 1985934

DOI: 10.1016/s0021-9258(17)35283-3

PubMed ID: 8202360

Title: Specificities of human, rat and E. coli O6-methylguanine-DNA methyltransferases towards the repair of O6-methyl and O6-ethylguanine in DNA.

PubMed ID: 8202360

DOI: 10.1093/nar/22.9.1613

PubMed ID: 7954470

Title: Mutations in human O6-alkylguanine-DNA alkyltransferase imparting resistance to O6-benzylguanine.

PubMed ID: 7954470

PubMed ID: 7766621

Title: Alteration of arginine-128 to alanine abolishes the ability of human O6-alkylguanine-DNA alkyltransferase to repair methylated DNA but has no effect on its reaction with O6-benzylguanine.

PubMed ID: 7766621

DOI: 10.1021/bi00021a024

PubMed ID: 7614699

Title: The role of tyrosine-158 in O6-alkylguanine-DNA alkyltransferase activity.

PubMed ID: 7614699

DOI: 10.1093/carcin/16.7.1637

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 10747039

Title: Active and alkylated human AGT structures: a novel zinc site, inhibitor and extrahelical base binding.

PubMed ID: 10747039

DOI: 10.1093/emboj/19.7.1719

PubMed ID: 10606635

Title: Crystal structure of the human O(6)-alkylguanine-DNA alkyltransferase.

PubMed ID: 10606635

DOI: 10.1093/nar/28.2.393

PubMed ID: 15964013

Title: The structure of the human AGT protein bound to DNA and its implications for damage detection.

PubMed ID: 15964013

DOI: 10.1016/j.jmb.2005.05.028

Sequence Information:

  • Length: 207
  • Mass: 21646
  • Checksum: 76BBF19DFC4512D6
  • Sequence:
    • MDKDCEMKRT TLDSPLGKLE LSGCEQGLHE IKLLGKGTSA ADAVEVPAPA AVLGGPEPLM 
QCTAWLNAYF HQPEAIEEFP VPALHHPVFQ QESFTRQVLW KLLKVVKFGE VISYQQLAAL 
AGNPKAARAV GGAMRGNPVP ILIPCHRVVC SSGAVGNYSG GLAVKEWLLA HEGHRLGKPG 
LGGSSGLAGA WLKGAGATSG SPPAGRN

Genular Protein ID: 799171085

Symbol: B4DEE8_HUMAN

Name: N/A

UniProtKB Accession Codes:

B4DEE8

Database IDs:

ARBA 18 Antibodypedia 1 Bgee 1 BioGRID-ORCS 1 CDD 1 ChEBI 9 ChiTaRS 1 DNASU 1 EC 2 EMBL 5 EPD 1 Ensembl 2 GO 5 Gene3D 2 GeneTree 1 Genevisible 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 InterPro 6 MassIVE 1 MaxQB 1 NCBI Taxonomy 1 NCBIfam 1 PANTHER 2 PROSITE 1 Pfam 4 PharmGKB 1 Proteomes 2 RefSeq 1 Rhea 10 SUPFAM 2 VEuPathDB 1

Citations:

PubMed ID: 15164054

Title: The DNA sequence and comparative analysis of human chromosome 10.

PubMed ID: 15164054

DOI: 10.1038/nature02462

Sequence Information:

  • Length: 238
  • Mass: 25050
  • Checksum: 9A1774B6637A0788
  • Sequence:
    • MLGQPAPLER FASRRPQVLA VRTVCDLVLG KMDKDCEMKR TTLDSPLGKL ELSGCEQGLH 
EIKLLGKGTS AADAVEVPAP AAVLGGPEPL MQCTAWLNAY FHQPEAIEEF PVPALHHPVF 
QQESFTRQVL WKLLKVVKFG EVISYQQLAA LAGNPKAARA VGGAMRGNPV PILIPCHRVV 
CSSGAVGNYS GGLAVKEWLL AHEGHRLGKP GLGGSSGLAG AWLKGAGATS GSPPAGRN

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. For the full schema, download it here.