Details for: NEDD8

Gene ID: 4738

Symbol: NEDD8

Ensembl ID: ENSG00000129559

Description: NEDD8 ubiquitin like modifier

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 539.5858
    Cell Significance Index: -83.9300
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 321.5933
    Cell Significance Index: -81.5700
  • Cell Name: embryonic stem cell (CL0002322)
    Fold Change: 253.6973
    Cell Significance Index: -104.5100
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 231.0417
    Cell Significance Index: -109.0800
  • Cell Name: peripheral blood mononuclear cell (CL2000001)
    Fold Change: 197.4590
    Cell Significance Index: -101.5700
  • Cell Name: ileal goblet cell (CL1000326)
    Fold Change: 163.5415
    Cell Significance Index: -109.7400
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 81.5966
    Cell Significance Index: -100.6100
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 27.5212
    Cell Significance Index: -108.6000
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 26.1830
    Cell Significance Index: -80.4200
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 17.8173
    Cell Significance Index: -47.7300
  • Cell Name: obsolete epithelial cell of alveolus of lung (CL0010003)
    Fold Change: 9.6411
    Cell Significance Index: 240.4200
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: 3.5669
    Cell Significance Index: 74.6600
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 3.3702
    Cell Significance Index: 98.9800
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 3.0702
    Cell Significance Index: 421.6300
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 3.0239
    Cell Significance Index: 140.9900
  • Cell Name: endothelial cell of venule (CL1000414)
    Fold Change: 2.9876
    Cell Significance Index: 33.9400
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 2.9537
    Cell Significance Index: 363.1800
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: 2.9439
    Cell Significance Index: 77.4100
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: 2.9286
    Cell Significance Index: 137.6400
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 2.8220
    Cell Significance Index: 508.7100
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 2.7591
    Cell Significance Index: 1506.8100
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 2.6607
    Cell Significance Index: 139.7000
  • Cell Name: kidney cell (CL1000497)
    Fold Change: 2.6126
    Cell Significance Index: 20.8600
  • Cell Name: umbrella cell of urothelium (CL4030056)
    Fold Change: 2.2099
    Cell Significance Index: 20.3500
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 2.1684
    Cell Significance Index: 139.9000
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 1.9938
    Cell Significance Index: 881.5000
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: 1.9203
    Cell Significance Index: 52.2700
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: 1.8877
    Cell Significance Index: 140.6900
  • Cell Name: odontoblast (CL0000060)
    Fold Change: 1.8442
    Cell Significance Index: 236.4200
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 1.6004
    Cell Significance Index: 113.1900
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 1.5947
    Cell Significance Index: 188.0600
  • Cell Name: peg cell (CL4033014)
    Fold Change: 1.5621
    Cell Significance Index: 36.0900
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 1.4959
    Cell Significance Index: 193.2600
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 1.2751
    Cell Significance Index: 66.2400
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.9587
    Cell Significance Index: 192.3100
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: 0.9488
    Cell Significance Index: 32.9700
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.9071
    Cell Significance Index: 89.7300
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: 0.8792
    Cell Significance Index: 16.2500
  • Cell Name: skeletal muscle myoblast (CL0000515)
    Fold Change: 0.8693
    Cell Significance Index: 9.4500
  • Cell Name: vascular lymphangioblast (CL0005022)
    Fold Change: 0.7682
    Cell Significance Index: 13.5800
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: 0.5248
    Cell Significance Index: 151.0100
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.4491
    Cell Significance Index: 76.6800
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.4027
    Cell Significance Index: 8.7300
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.3406
    Cell Significance Index: 122.1600
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: 0.2542
    Cell Significance Index: 192.4100
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.2377
    Cell Significance Index: 45.2400
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.1332
    Cell Significance Index: 2.2300
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.1307
    Cell Significance Index: 3.4900
  • Cell Name: fibro/adipogenic progenitor cell (CL0009099)
    Fold Change: 0.1071
    Cell Significance Index: 5.4100
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: 0.0963
    Cell Significance Index: 70.5900
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0083
    Cell Significance Index: -6.1200
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0509
    Cell Significance Index: -5.2000
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0565
    Cell Significance Index: -106.2900
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0578
    Cell Significance Index: -36.0800
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0704
    Cell Significance Index: -129.9000
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0843
    Cell Significance Index: -129.7600
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0960
    Cell Significance Index: -130.5300
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.1150
    Cell Significance Index: -64.8600
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: -0.1492
    Cell Significance Index: -94.7300
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: -0.1605
    Cell Significance Index: -4.6300
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: -0.1674
    Cell Significance Index: -4.8000
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.1944
    Cell Significance Index: -40.9400
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.2108
    Cell Significance Index: -24.1500
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.2368
    Cell Significance Index: -107.4600
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.2640
    Cell Significance Index: -30.7700
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: -0.3655
    Cell Significance Index: -59.4400
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: -0.4149
    Cell Significance Index: -14.5800
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: -0.4393
    Cell Significance Index: -27.6900
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: -0.4427
    Cell Significance Index: -11.3100
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: -0.4839
    Cell Significance Index: -96.0300
  • Cell Name: neuron associated cell (CL0000095)
    Fold Change: -0.5442
    Cell Significance Index: -22.3000
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.6768
    Cell Significance Index: -53.6000
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.7309
    Cell Significance Index: -106.2400
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: -0.7937
    Cell Significance Index: -54.8900
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: -0.8581
    Cell Significance Index: -18.2800
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: -0.8741
    Cell Significance Index: -39.6200
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: -0.8889
    Cell Significance Index: -28.4700
  • Cell Name: pancreatic endocrine cell (CL0008024)
    Fold Change: -0.8901
    Cell Significance Index: -101.6000
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: -0.9746
    Cell Significance Index: -74.7900
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: -1.0193
    Cell Significance Index: -57.2000
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -1.0533
    Cell Significance Index: -109.6700
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: -1.1941
    Cell Significance Index: -33.3700
  • Cell Name: endothelial cell of placenta (CL0009092)
    Fold Change: -1.3183
    Cell Significance Index: -7.9700
  • Cell Name: paneth cell of colon (CL0009009)
    Fold Change: -1.4282
    Cell Significance Index: -21.4000
  • Cell Name: luminal hormone-sensing cell of mammary gland (CL4033058)
    Fold Change: -1.5619
    Cell Significance Index: -9.6100
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: -1.7201
    Cell Significance Index: -46.0900
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -1.7392
    Cell Significance Index: -106.6300
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: -1.7413
    Cell Significance Index: -51.2900
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: -1.8762
    Cell Significance Index: -82.9900
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: -1.9401
    Cell Significance Index: -26.4700
  • Cell Name: microcirculation associated smooth muscle cell (CL0008035)
    Fold Change: -2.1167
    Cell Significance Index: -17.7800
  • Cell Name: chandelier pvalb GABAergic cortical interneuron (CL4023036)
    Fold Change: -2.1336
    Cell Significance Index: -44.5300
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: -2.2325
    Cell Significance Index: -84.5400
  • Cell Name: cerebral cortex endothelial cell (CL1001602)
    Fold Change: -2.2813
    Cell Significance Index: -46.4200
  • Cell Name: L5 extratelencephalic projecting glutamatergic cortical neuron (CL4023041)
    Fold Change: -2.3132
    Cell Significance Index: -81.0400
  • Cell Name: L6b glutamatergic cortical neuron (CL4023038)
    Fold Change: -2.3488
    Cell Significance Index: -76.9000
  • Cell Name: CD4-positive, alpha-beta thymocyte (CL0000810)
    Fold Change: -2.3929
    Cell Significance Index: -41.2500
  • Cell Name: stratified epithelial cell (CL0000079)
    Fold Change: -2.4250
    Cell Significance Index: -89.0200
  • Cell Name: interstitial cell of ovary (CL0002094)
    Fold Change: -2.4745
    Cell Significance Index: -31.6900
  • Cell Name: corticothalamic-projecting glutamatergic cortical neuron (CL4023013)
    Fold Change: -2.6356
    Cell Significance Index: -83.9400

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** NEDD8 is a 7-kDa protein that shares structural similarity with ubiquitin, but its function is distinct from that of ubiquitin. NEDD8 is synthesized by the enzyme NEDD8-activating enzyme (UBE2M2) and conjugated to target proteins by the E3 ubiquitin ligase UBE2M2. NEDD8 is a specific and reversible modifier, which can be removed by the deubiquitinase UCH11. The neddylation pathway is essential for the activation of proteins involved in various cellular processes, including cell cycle regulation, apoptosis, and immune responses. **Pathways and Functions:** NEDD8 is involved in multiple signaling pathways, including: 1. **Regulation of Proteolysis:** NEDD8 is a key regulator of proteolysis, particularly in the ubiquitin-proteasome pathway. It interacts with components of the proteasome, such as PSME3 and PSME4, and influences the degradation of target proteins. 2. **Cell Cycle Regulation:** NEDD8 is involved in the regulation of cell cycle progression, particularly in the G2/M phase. It interacts with proteins involved in the mitotic spindle assembly and cytokinesis. 3. **Apoptosis:** NEDD8 is a pro-apoptotic protein, which promotes cell death by regulating the activity of pro-apoptotic proteins, such as BAX and BAK. 4. **Immune Response:** NEDD8 is involved in the regulation of immune responses, particularly in the activation of dendritic cells and T cells. 5. **Signaling by TGF-β Receptor Complex:** NEDD8 interacts with components of the TGF-β receptor complex and influences the activation of Smad proteins. **Clinical Significance:** Dysregulation of NEDD8 has been implicated in various disease states, including: 1. **Cancer:** Overexpression of NEDD8 has been observed in several types of cancer, including breast, lung, and colon cancer. NEDD8 promotes tumor growth and metastasis by regulating the activity of oncogenic proteins. 2. **Autoimmune Diseases:** NEDD8 is involved in the regulation of immune responses, and dysregulation of NEDD8 has been implicated in autoimmune diseases, such as rheumatoid arthritis and lupus. 3. **Neurological Disorders:** NEDD8 has been implicated in the regulation of neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. 4. **Infectious Diseases:** NEDD8 is involved in the regulation of immune responses to infections, and dysregulation of NEDD8 has been implicated in diseases, such as tuberculosis and HIV. In conclusion, NEDD8 is a multifunctional protein that plays a crucial role in various cellular processes, including protein modification, regulation of proteolysis, and signaling pathways. Dysregulation of NEDD8 has been implicated in various disease states, highlighting the importance of NEDD8 in maintaining cellular homeostasis and preventing disease.

Genular Protein ID: 954541440

Symbol: NEDD8_HUMAN

Name: NEDD8

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 9694792

Title: A new NEDD8-ligating system for cullin-4A.

PubMed ID: 9694792

DOI: 10.1101/gad.12.15.2263

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 10318914

Title: Conjugation of the ubiquitin-like protein NEDD8 to cullin-2 is linked to von Hippel-Lindau tumor suppressor function.

PubMed ID: 10318914

DOI: 10.1073/pnas.96.10.5510

PubMed ID: 9353319

Title: Characterization of NEDD8, a developmentally down-regulated ubiquitin-like protein.

PubMed ID: 9353319

DOI: 10.1074/jbc.272.45.28557

PubMed ID: 9790970

Title: Cleavage of the C-terminus of NEDD8 by UCH-L3.

PubMed ID: 9790970

DOI: 10.1006/bbrc.1998.9532

PubMed ID: 10597293

Title: Covalent modification of all members of human cullin family proteins by NEDD8.

PubMed ID: 10597293

DOI: 10.1038/sj.onc.1203093

PubMed ID: 11259415

Title: NUB1, a NEDD8-interacting protein, is induced by interferon and down-regulates the NEDD8 expression.

PubMed ID: 11259415

DOI: 10.1074/jbc.m100920200

PubMed ID: 11953428

Title: The NEDD8 pathway is essential for SCF(beta -TrCP)-mediated ubiquitination and processing of the NF-kappa B precursor p105.

PubMed ID: 11953428

DOI: 10.1074/jbc.m200967200

PubMed ID: 12215427

Title: Interaction with Nedd8, a ubiquitin-like protein, enhances the transcriptional activity of the aryl hydrocarbon receptor.

PubMed ID: 12215427

DOI: 10.1074/jbc.m202413200

PubMed ID: 12730221

Title: NEDP1, a highly conserved cysteine protease that deneddylates cullins.

PubMed ID: 12730221

DOI: 10.1074/jbc.m212948200

PubMed ID: 12759363

Title: DEN1 is a dual function protease capable of processing the C-terminus of Nedd8 and deconjugating hyper-neddylated CUL1.

PubMed ID: 12759363

DOI: 10.1074/jbc.m302888200

PubMed ID: 15242646

Title: Mdm2-mediated NEDD8 conjugation of p53 inhibits its transcriptional activity.

PubMed ID: 15242646

DOI: 10.1016/j.cell.2004.06.016

PubMed ID: 20850415

Title: The bacterial effector Cif interferes with SCF ubiquitin ligase function by inhibiting deneddylation of Cullin1.

PubMed ID: 20850415

DOI: 10.1016/j.bbrc.2010.09.048

PubMed ID: 20688984

Title: Glutamine deamidation and dysfunction of ubiquitin/NEDD8 induced by a bacterial effector family.

PubMed ID: 20688984

DOI: 10.1126/science.1193844

PubMed ID: 21903097

Title: Inhibition of cullin RING ligases by cycle inhibiting factor: evidence for interference with Nedd8-induced conformational control.

PubMed ID: 21903097

DOI: 10.1016/j.jmb.2011.08.030

PubMed ID: 23589306

Title: The cyclomodulin cycle inhibiting factor (CIF) alters cullin neddylation dynamics.

PubMed ID: 23589306

DOI: 10.1074/jbc.m112.448258

PubMed ID: 26632597

Title: Gln40 deamidation blocks structural reconfiguration and activation of SCF ubiquitin ligase complex by Nedd8.

PubMed ID: 26632597

DOI: 10.1038/ncomms10053

PubMed ID: 9857030

Title: Crystal structure of the human ubiquitin-like protein NEDD8 and interactions with ubiquitin pathway enzymes.

PubMed ID: 9857030

DOI: 10.1074/jbc.273.52.34983

PubMed ID: 14690597

Title: The structure of the APPBP1-UBA3-NEDD8-ATP complex reveals the basis for selective ubiquitin-like protein activation by an E1.

PubMed ID: 14690597

DOI: 10.1016/s1097-2765(03)00452-0

PubMed ID: 15567417

Title: Structure of a complex between Nedd8 and the Ulp/Senp protease family member Den1.

PubMed ID: 15567417

DOI: 10.1016/j.jmb.2004.10.022

PubMed ID: 22691497

Title: The molecular basis of ubiquitin-like protein NEDD8 deamidation by the bacterial effector protein Cif.

PubMed ID: 22691497

DOI: 10.1073/pnas.1112107109

PubMed ID: 23175788

Title: Structural mechanism of ubiquitin and NEDD8 deamidation catalyzed by bacterial effectors that induce macrophage-specific apoptosis.

PubMed ID: 23175788

DOI: 10.1073/pnas.1210831109

Sequence Information:

  • Length: 81
  • Mass: 9072
  • Checksum: DC2FE102BE4725D2
  • Sequence:
  • MLIKVKTLTG KEIEIDIEPT DKVERIKERV EEKEGIPPQQ QRLIYSGKQM NDEKTAADYK 
    ILGGSVLHLV LALRGGGGLR Q

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.