Details for: DHFR
Gene ID: 1719
Gene Type: Protein-coding - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.
Symbol: DHFR
Ensembl ID: ENSG00000228716
Description: dihydrofolate reductase
Selected Context(s): Overall
Cell Significance Landscape
Associated with
Significant Cells
Cell Significance Index (CSI) scores for the chosen context(s)
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CSI 33.67rCSI 29.49%PRS 88.89
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CSI 29.87rCSI 42.87%PRS 72.67
-
CSI 13.77rCSI 13.26%PRS 81.86
-
CSI 12.95rCSI 26.88%PRS 79.36
-
CSI 12.73rCSI 36.35%PRS 86.78
-
CSI 11.54rCSI 21.79%PRS 89.44
-
CSI 11.08rCSI 9.83%PRS 80.26
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CSI 11rCSI 18.02%PRS 72.38
-
CSI 9.99rCSI 22.67%PRS 82.62
-
CSI 9.76rCSI 11.79%PRS 89.17
-
CSI 9.65rCSI 12.29%PRS 87.17
-
CSI 9.21rCSI 11.39%PRS 80.64
-
CSI 9.04rCSI 12.35%PRS 86.68
-
CSI 8.96rCSI 8.46%PRS 80.87
-
CSI 8.49rCSI 11.8%PRS 80.8
-
CSI 8.24rCSI 6.51%PRS 72.1
-
CSI 6.95rCSI 6.28%PRS 80.76
-
CSI 6.84rCSI 30.03%PRS 89.17
-
CSI 6.53rCSI 7.67%PRS 83.9
-
CSI 6.53rCSI 46.18%PRS 75.45
-
CSI 6.3rCSI 23.61%PRS 75.34
-
CSI 6.19rCSI 5.13%PRS 84.5
-
CSI 6.07rCSI 7.01%PRS 74.74
-
CSI 5.84rCSI 4.72%PRS 84.55
-
CSI 5.73rCSI 30.94%PRS 87.63
-
CSI 5.52rCSI 6.93%PRS 89.87
-
CSI 5.33rCSI 14.14%PRS 87.01
-
CSI 5.25rCSI 4.13%PRS 86.92
-
CSI 5.08rCSI 5.32%PRS 79.93
-
CSI 4.8rCSI 23.68%PRS 89.04
-
CSI 4.77rCSI 22.86%PRS 78.8
-
CSI 4.56rCSI 6.97%PRS 89.53
-
CSI 4.51rCSI 12.01%PRS 60.47
-
CSI 4.03rCSI 3.54%PRS 87.2
-
CSI 4rCSI 3.99%PRS 76.46
-
CSI 3.91rCSI 6.23%PRS 74.11
-
CSI 3.72rCSI 4.62%PRS 63.07
-
CSI 3.69rCSI 4.22%PRS 91.33
-
CSI 3.67rCSI 14.95%PRS 74.32
-
CSI 3.51rCSI 3.66%PRS 80.06
-
CSI 3.44rCSI 8.08%PRS 82.05
-
CSI 3.37rCSI 4.86%PRS 87.45
-
CSI 3.37rCSI 2.92%PRS 86.18
-
CSI 3.34rCSI 12.97%PRS 81.48
-
CSI 3.32rCSI 14.1%PRS 74.95
-
CSI 3.3rCSI 2.74%PRS 83.41
-
CSI 3.28rCSI 3.92%PRS 65.29
-
CSI 3.21rCSI 2.6%PRS 81.91
-
CSI 3.03rCSI 2.92%PRS 76.45
-
CSI 2.99rCSI 2.09%PRS 85.9
-
CSI 2.9rCSI 3.72%PRS 78.58
-
CSI 2.86rCSI 6.85%PRS 69.54
-
CSI 2.79rCSI 6.37%PRS 83.22
-
CSI 2.66rCSI 6.13%PRS 70.79
-
CSI 2.55rCSI 4.36%PRS 87.64
-
CSI 2.52rCSI 2.32%PRS 89.36
-
CSI 2.5rCSI 3.86%PRS 81.5
-
CSI 2.25rCSI 6.94%PRS 86.23
-
CSI 2.23rCSI 14.34%PRS 89.77
-
CSI 2.21rCSI 53.39%PRS 63.73
-
CSI 2.2rCSI 2.83%PRS 72.87
-
CSI 2.16rCSI 2.2%PRS 90.8
-
CSI 2.14rCSI 51.12%PRS 63.15
-
CSI 2.1rCSI 2.52%PRS 80.55
-
CSI 2.1rCSI 3.75%PRS 81.74
-
CSI 2.03rCSI 17.51%PRS 76.82
-
CSI 1.88rCSI 2.99%PRS 75.92
-
CSI 1.85rCSI 5.27%PRS 78.1
-
CSI 1.85rCSI 5.31%PRS 76.29
-
CSI 1.79rCSI 2.4%PRS 94.67
-
CSI 1.79rCSI 4.65%PRS 82.55
-
CSI 1.76rCSI 1.82%PRS 85.92
-
CSI 1.68rCSI 2.16%PRS 66.34
-
CSI 1.6rCSI 2.53%PRS 83.85
-
CSI 1.59rCSI 3.45%PRS 70
-
CSI 1.54rCSI 6.02%PRS 61.58
-
CSI 1.46rCSI 3.57%PRS 74.92
-
CSI 1.46rCSI 2.41%PRS 85.93
-
CSI 1.46rCSI 8.37%PRS 86.88
-
CSI 1.4rCSI 4.96%PRS 96.8
-
CSI 1.36rCSI 4.35%PRS 83.64
-
CSI 1.35rCSI 2.38%PRS 64.69
-
CSI 1.23rCSI 3.28%PRS 74.7
-
CSI 1.22rCSI 3.18%PRS 80.64
-
CSI 1.21rCSI 3.99%PRS 68.43
-
CSI 1.21rCSI 3.61%PRS 90.52
-
CSI 1.16rCSI 2.82%PRS 63.1
-
CSI 1.15rCSI 2.92%PRS 73.3
-
CSI 1.09rCSI 2.82%PRS 78.06
-
CSI 1.03rCSI 1.5%PRS 77.27
-
CSI 1rCSI 9.49%PRS 95.85
-
CSI 0.99rCSI 2.26%PRS 75.98
-
CSI 0.88rCSI 4.57%PRS 94.43
-
CSI 0.81rCSI 2.75%PRS 79.13
-
CSI 0.77rCSI 6.25%PRS 77.15
-
CSI 0.74rCSI 3.28%PRS 83
-
CSI 0.73rCSI 2.83%PRS 94.84
-
CSI 0.71rCSI 1.82%PRS 76.24
-
CSI 0.61rCSI 4%PRS 95.2
-
CSI 0.57rCSI 17.02%PRS 91.22
-
CSI 0.3rCSI 3.5%PRS 95.3%
-
CSI 0.4rCSI 3.8%PRS 78.5%
-
CSI 0.6rCSI 17.0%PRS 91.2%
-
CSI 0.6rCSI 4.0%PRS 95.2%
-
CSI 0.7rCSI 1.8%PRS 76.2%
-
CSI 0.7rCSI 2.8%PRS 94.8%
-
CSI 0.7rCSI 3.3%PRS 83.0%
-
CSI 0.8rCSI 6.3%PRS 77.2%
-
CSI 0.8rCSI 2.8%PRS 79.1%
-
CSI 0.9rCSI 4.6%PRS 94.4%
-
CSI 1.0rCSI 2.3%PRS 76.0%
-
CSI 1.0rCSI 9.5%PRS 95.9%
-
CSI 1.0rCSI 1.5%PRS 77.3%
-
CSI 1.1rCSI 2.8%PRS 78.1%
-
CSI 1.2rCSI 2.9%PRS 73.3%
-
CSI 1.2rCSI 2.8%PRS 63.1%
-
CSI 1.2rCSI 3.6%PRS 90.5%
-
CSI 1.2rCSI 4.0%PRS 68.4%
-
CSI 1.2rCSI 3.2%PRS 80.6%
-
CSI 1.2rCSI 3.3%PRS 74.7%
-
CSI 1.4rCSI 2.4%PRS 64.7%
-
CSI 1.4rCSI 4.4%PRS 83.6%
-
CSI 1.4rCSI 5.0%PRS 96.8%
-
CSI 1.5rCSI 8.4%PRS 86.9%
-
CSI 1.5rCSI 2.4%PRS 85.9%
-
CSI 1.5rCSI 3.6%PRS 74.9%
-
CSI 1.5rCSI 6.0%PRS 61.6%
-
CSI 1.6rCSI 3.5%PRS 70.0%
-
CSI 1.6rCSI 2.5%PRS 83.9%
-
CSI 1.7rCSI 2.2%PRS 66.3%
-
CSI 1.8rCSI 1.8%PRS 85.9%
-
CSI 1.8rCSI 4.7%PRS 82.6%
-
CSI 1.8rCSI 2.4%PRS 94.7%
-
CSI 1.9rCSI 5.3%PRS 76.3%
-
CSI 1.9rCSI 5.3%PRS 78.1%
-
CSI 1.9rCSI 3.0%PRS 75.9%
-
CSI 2.0rCSI 17.5%PRS 76.8%
-
CSI 2.1rCSI 3.8%PRS 81.7%
-
CSI 2.1rCSI 2.5%PRS 80.6%
-
CSI 2.1rCSI 51.1%PRS 63.2%
-
CSI 2.2rCSI 2.2%PRS 90.8%
-
CSI 2.2rCSI 2.8%PRS 72.9%
-
CSI 2.2rCSI 53.4%PRS 63.7%
-
CSI 2.2rCSI 14.3%PRS 89.8%
-
CSI 2.3rCSI 6.9%PRS 86.2%
-
CSI 2.5rCSI 3.9%PRS 81.5%
-
CSI 2.5rCSI 2.3%PRS 89.4%
-
CSI 2.6rCSI 4.4%PRS 87.6%
-
CSI 2.7rCSI 6.1%PRS 70.8%
-
CSI 2.8rCSI 6.4%PRS 83.2%
-
CSI 2.9rCSI 6.9%PRS 69.5%
-
CSI 2.9rCSI 3.7%PRS 78.6%
-
CSI 3.0rCSI 2.1%PRS 85.9%
-
CSI 3.0rCSI 2.9%PRS 76.5%
-
CSI 3.2rCSI 2.6%PRS 81.9%
-
CSI 3.3rCSI 3.9%PRS 65.3%
-
CSI 3.3rCSI 2.7%PRS 83.4%
-
CSI 3.3rCSI 14.1%PRS 75.0%
-
CSI 3.3rCSI 13.0%PRS 81.5%
-
CSI 3.4rCSI 2.9%PRS 86.2%
-
CSI 3.4rCSI 4.9%PRS 87.5%
-
CSI 3.4rCSI 8.1%PRS 82.1%
-
CSI 3.5rCSI 3.7%PRS 80.1%
-
CSI 3.7rCSI 15.0%PRS 74.3%
-
CSI 3.7rCSI 4.2%PRS 91.3%
-
CSI 3.7rCSI 4.6%PRS 63.1%
-
CSI 3.9rCSI 6.2%PRS 74.1%
-
CSI 4.0rCSI 4.0%PRS 76.5%
-
CSI 4.0rCSI 3.5%PRS 87.2%
-
CSI 4.5rCSI 12.0%PRS 60.5%
-
CSI 4.6rCSI 7.0%PRS 89.5%
-
CSI 4.8rCSI 22.9%PRS 78.8%
-
CSI 4.8rCSI 23.7%PRS 89.0%
-
CSI 5.1rCSI 5.3%PRS 79.9%
-
CSI 5.3rCSI 4.1%PRS 86.9%
-
CSI 5.3rCSI 14.1%PRS 87.0%
-
CSI 5.5rCSI 6.9%PRS 89.9%
-
CSI 5.7rCSI 30.9%PRS 87.6%
-
CSI 5.8rCSI 4.7%PRS 84.6%
-
CSI 6.1rCSI 7.0%PRS 74.7%
-
CSI 6.2rCSI 5.1%PRS 84.5%
-
CSI 6.3rCSI 23.6%PRS 75.3%
-
CSI 6.5rCSI 46.2%PRS 75.5%
-
CSI 6.5rCSI 7.7%PRS 83.9%
-
CSI 6.8rCSI 30.0%PRS 89.2%
-
CSI 7.0rCSI 6.3%PRS 80.8%
-
CSI 8.2rCSI 6.5%PRS 72.1%
-
CSI 8.5rCSI 11.8%PRS 80.8%
-
CSI 9.0rCSI 8.5%PRS 80.9%
-
CSI 9.0rCSI 12.4%PRS 86.7%
-
CSI 9.2rCSI 11.4%PRS 80.6%
-
CSI 9.7rCSI 12.3%PRS 87.2%
-
CSI 9.8rCSI 11.8%PRS 89.2%
-
CSI 10.0rCSI 22.7%PRS 82.6%
-
CSI 11.0rCSI 18.0%PRS 72.4%
-
CSI 11.1rCSI 9.8%PRS 80.3%
-
CSI 11.5rCSI 21.8%PRS 89.4%
-
CSI 12.7rCSI 36.4%PRS 86.8%
-
CSI 13.0rCSI 26.9%PRS 79.4%
-
CSI 13.8rCSI 13.3%PRS 81.9%
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration
Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.
Legend:
- Query Gene
-
Node Color (Target Cell CSI, relative to current network):
- Very High
- High
- Medium
- Low
- Very Low
- CSI N/A
- Node Size: Proportional to Target Cell CSI magnitude
- STRING PPI Edge
- Shared Pathway Edge (ONTOLOGY)
Other Information
This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.
Genular Protein ID: 4053884756
Symbol: DYR_HUMAN
Name: Dihydrofolate reductase
UniProtKB Accession Codes:
Database IDs:
Citations:
PubMed ID: 6323448
Title: The functional human dihydrofolate reductase gene.
PubMed ID: 6323448
PubMed ID: 6687716
Title: The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase.
PubMed ID: 6687716
PubMed ID: 6235374
Title: Human dihydrofolate reductase gene organization. Extensive conservation of the G + C-rich 5' non-coding sequence and strong intron size divergence from homologous mammalian genes.
PubMed ID: 6235374
PubMed ID: 14702039
Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.
PubMed ID: 14702039
DOI: 10.1038/ng1285
PubMed ID: 15372022
Title: The DNA sequence and comparative analysis of human chromosome 5.
PubMed ID: 15372022
DOI: 10.1038/nature02919
PubMed ID: 15489334
Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).
PubMed ID: 15489334
DOI: 10.1101/gr.2596504
PubMed ID: 21310276
Title: Identification and characterization of an inborn error of metabolism caused by dihydrofolate reductase deficiency.
PubMed ID: 21310276
PubMed ID: 21269460
Title: Initial characterization of the human central proteome.
PubMed ID: 21269460
PubMed ID: 21876184
Title: The former annotated human pseudogene dihydrofolate reductase-like 1 (DHFRL1) is expressed and functional.
PubMed ID: 21876184
PubMed ID: 21876188
Title: Identification of a de novo thymidylate biosynthesis pathway in mammalian mitochondria.
PubMed ID: 21876188
PubMed ID: 3383852
Title: Crystal structure of human dihydrofolate reductase complexed with folate.
PubMed ID: 3383852
PubMed ID: 2248959
Title: Crystal structures of recombinant human dihydrofolate reductase complexed with folate and 5-deazafolate.
PubMed ID: 2248959
DOI: 10.1021/bi00492a021
PubMed ID: 1731871
Title: Sequence-specific 1H and 15N resonance assignments for human dihydrofolate reductase in solution.
PubMed ID: 1731871
DOI: 10.1021/bi00116a031
PubMed ID: 7890613
Title: Methotrexate-resistant variants of human dihydrofolate reductase with substitutions of leucine 22. Kinetics, crystallography, and potential as selectable markers.
PubMed ID: 7890613
PubMed ID: 9374868
Title: Comparison of two independent crystal structures of human dihydrofolate reductase ternary complexes reduced with nicotinamide adenine dinucleotide phosphate and the very tight-binding inhibitor PT523.
PubMed ID: 9374868
DOI: 10.1021/bi971711l
PubMed ID: 9719595
Title: Structure-based design and synthesis of lipophilic 2,4-diamino-6-substituted quinazolines and their evaluation as inhibitors of dihydrofolate reductases and potential antitumor agents.
PubMed ID: 9719595
DOI: 10.1021/jm980081y
PubMed ID: 12096917
Title: Atomic structures of human dihydrofolate reductase complexed with NADPH and two lipophilic antifolates at 1.09 A and 1.05 A resolution.
PubMed ID: 12096917
PubMed ID: 12657784
Title: Analysis of two polymorphic forms of a pyrido[2,3-d]pyrimidine N9-C10 reversed-bridge antifolate binary complex with human dihydrofolate reductase.
PubMed ID: 12657784
PubMed ID: 12925791
Title: Analysis of three crystal structure determinations of a 5-methyl-6-N-methylanilino pyridopyrimidine antifolate complex with human dihydrofolate reductase.
PubMed ID: 12925791
PubMed ID: 15039552
Title: Structure determination of tetrahydroquinazoline antifolates in complex with human and Pneumocystis carinii dihydrofolate reductase: correlations between enzyme selectivity and stereochemistry.
PubMed ID: 15039552
PubMed ID: 15681865
Title: Understanding the role of Leu22 variants in methotrexate resistance: comparison of wild-type and Leu22Arg variant mouse and human dihydrofolate reductase ternary crystal complexes with methotrexate and NADPH.
PubMed ID: 15681865
PubMed ID: 16222560
Title: Solution structure of human dihydrofolate reductase in its complex with trimethoprim and NADPH.
PubMed ID: 16222560
PubMed ID: 17569517
Title: Novel boron-containing, nonclassical antifolates: synthesis and preliminary biological and structural evaluation.
PubMed ID: 17569517
DOI: 10.1021/jm0701977
PubMed ID: 19196009
Title: Correlations of inhibitor kinetics for Pneumocystis jirovecii and human dihydrofolate reductase with structural data for human active site mutant enzyme complexes.
PubMed ID: 19196009
DOI: 10.1021/bi801960h
PubMed ID: 19478082
Title: Multiple conformers in active site of human dihydrofolate reductase F31R/Q35E double mutant suggest structural basis for methotrexate resistance.
PubMed ID: 19478082
PubMed ID: 19719239
Title: Design, synthesis, and X-ray crystal structure of classical and nonclassical 2-amino-4-oxo-5-substituted-6-ethylthieno[2,3-d]pyrimidines as dual thymidylate synthase and dihydrofolate reductase inhibitors and as potential antitumor agents.
PubMed ID: 19719239
DOI: 10.1021/jm900490a
PubMed ID: 21310277
Title: Dihydrofolate reductase deficiency due to a homozygous DHFR mutation causes megaloblastic anemia and cerebral folate deficiency leading to severe neurologic disease.
PubMed ID: 21310277
Sequence Information:
- Length: 187
- Mass: 21453
- Checksum: EBDF3D1EC73E1566
- Sequence:
MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKEAMNHPG HLKLFVTRIM QDFESDTFFP EIDLEKYKLL PEYPGVLSDV QEEKGIKYKF EVYEKND
Genular Protein ID: 3716210371
Symbol: B4DM58_HUMAN
Name: N/A
UniProtKB Accession Codes:
Database IDs:
Citations:
PubMed ID: 11237011
Title: Initial sequencing and analysis of the human genome.
PubMed ID: 11237011
DOI: 10.1038/35057062
PubMed ID: 15372022
Title: The DNA sequence and comparative analysis of human chromosome 5.
PubMed ID: 15372022
DOI: 10.1038/nature02919
PubMed ID: 15496913
Title: Finishing the euchromatic sequence of the human genome.
PubMed ID: 15496913
DOI: 10.1038/nature03001
PubMed ID: 21269460
Title: Initial characterization of the human central proteome.
PubMed ID: 21269460
Sequence Information:
- Length: 129
- Mass: 14500
- Checksum: A86D545B3347CB61
- Sequence:
MVGSLNCIVA VSQNMGIGKN GDLPWPPLRN EFRYFQRMTT TSSVEGKQNL VIMGKKTWFS IPEKNRPLKG RINLVLSREL KEPPQGAHFL SRSLDDALKL TEQPELANKV DMVWIVGGSS VYKIPRCSL