Details for: FEN1

Gene ID: 2237

Symbol: FEN1

Ensembl ID: ENSG00000168496

Description: flap structure-specific endonuclease 1

Associated with

Cells (max top 100)

(Marker Score score is uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: respiratory basal cell (CL0002633)
    Fold Change: 3.79
    Marker Score: 5449
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: 1.98
    Marker Score: 1938
  • Cell Name: oogonial cell (CL0000024)
    Fold Change: 1.45
    Marker Score: 2080
  • Cell Name: large pre-B-II cell (CL0000957)
    Fold Change: 1.42
    Marker Score: 3855
  • Cell Name: primitive red blood cell (CL0002355)
    Fold Change: 1.3
    Marker Score: 978.5
  • Cell Name: migratory enteric neural crest cell (CL0002607)
    Fold Change: 1.27
    Marker Score: 1197
  • Cell Name: cardiac endothelial cell (CL0010008)
    Fold Change: 1.22
    Marker Score: 2313
  • Cell Name: erythroblast (CL0000765)
    Fold Change: 1.21
    Marker Score: 758
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 1.18
    Marker Score: 3500
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: 1.14
    Marker Score: 4659
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 1.06
    Marker Score: 16600
  • Cell Name: primordial germ cell (CL0000670)
    Fold Change: 1.03
    Marker Score: 1293.5
  • Cell Name: cerebral cortex GABAergic interneuron (CL0010011)
    Fold Change: 1
    Marker Score: 71768
  • Cell Name: forebrain radial glial cell (CL0013000)
    Fold Change: 1
    Marker Score: 47991
  • Cell Name: absorptive cell (CL0000212)
    Fold Change: 0.98
    Marker Score: 30403
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 0.97
    Marker Score: 3829
  • Cell Name: intestinal crypt stem cell of colon (CL0009043)
    Fold Change: 0.95
    Marker Score: 2406
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.94
    Marker Score: 484
  • Cell Name: neural crest cell (CL0011012)
    Fold Change: 0.93
    Marker Score: 996
  • Cell Name: BEST4+ intestinal epithelial cell, human (CL4030026)
    Fold Change: 0.93
    Marker Score: 439
  • Cell Name: transit amplifying cell (CL0009010)
    Fold Change: 0.91
    Marker Score: 5208
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: 0.91
    Marker Score: 2718
  • Cell Name: fraction A pre-pro B cell (CL0002045)
    Fold Change: 0.9
    Marker Score: 908
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.88
    Marker Score: 353
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.87
    Marker Score: 5261
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 0.85
    Marker Score: 307
  • Cell Name: male germ cell (CL0000015)
    Fold Change: 0.85
    Marker Score: 243.5
  • Cell Name: immature innate lymphoid cell (CL0001082)
    Fold Change: 0.84
    Marker Score: 1717
  • Cell Name: plasmablast (CL0000980)
    Fold Change: 0.84
    Marker Score: 1119
  • Cell Name: intestinal epithelial cell (CL0002563)
    Fold Change: 0.78
    Marker Score: 1266
  • Cell Name: megakaryocyte progenitor cell (CL0000553)
    Fold Change: 0.76
    Marker Score: 213
  • Cell Name: IgG plasmablast (CL0000982)
    Fold Change: 0.75
    Marker Score: 206
  • Cell Name: Cajal-Retzius cell (CL0000695)
    Fold Change: 0.72
    Marker Score: 375
  • Cell Name: neuroblast (sensu Vertebrata) (CL0000031)
    Fold Change: 0.72
    Marker Score: 452
  • Cell Name: early T lineage precursor (CL0002425)
    Fold Change: 0.72
    Marker Score: 547
  • Cell Name: lymphoid lineage restricted progenitor cell (CL0000838)
    Fold Change: 0.72
    Marker Score: 430
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: 0.71
    Marker Score: 1214
  • Cell Name: epithelial cell of nephron (CL1000449)
    Fold Change: 0.71
    Marker Score: 1478
  • Cell Name: brush cell (CL0002204)
    Fold Change: 0.7
    Marker Score: 643
  • Cell Name: Unknown (CL0000548)
    Fold Change: 0.7
    Marker Score: 510
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: 0.69
    Marker Score: 207
  • Cell Name: smooth muscle myoblast (CL0000514)
    Fold Change: 0.69
    Marker Score: 328
  • Cell Name: neural progenitor cell (CL0011020)
    Fold Change: 0.67
    Marker Score: 2587
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.66
    Marker Score: 2767.5
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: 0.66
    Marker Score: 168
  • Cell Name: granulocyte monocyte progenitor cell (CL0000557)
    Fold Change: 0.65
    Marker Score: 410.5
  • Cell Name: early promyelocyte (CL0002154)
    Fold Change: 0.63
    Marker Score: 507
  • Cell Name: radial glial cell (CL0000681)
    Fold Change: 0.62
    Marker Score: 227
  • Cell Name: enteric smooth muscle cell (CL0002504)
    Fold Change: 0.59
    Marker Score: 1391
  • Cell Name: promonocyte (CL0000559)
    Fold Change: 0.58
    Marker Score: 374
  • Cell Name: neuronal receptor cell (CL0000006)
    Fold Change: 0.57
    Marker Score: 251
  • Cell Name: promyelocyte (CL0000836)
    Fold Change: 0.56
    Marker Score: 275
  • Cell Name: common myeloid progenitor (CL0000049)
    Fold Change: 0.55
    Marker Score: 147
  • Cell Name: intestinal enteroendocrine cell (CL1001516)
    Fold Change: 0.55
    Marker Score: 440
  • Cell Name: stem cell (CL0000034)
    Fold Change: 0.55
    Marker Score: 1301
  • Cell Name: ovarian surface epithelial cell (CL2000064)
    Fold Change: 0.54
    Marker Score: 1477
  • Cell Name: renal beta-intercalated cell (CL0002201)
    Fold Change: 0.54
    Marker Score: 170
  • Cell Name: osteoclast (CL0000092)
    Fold Change: 0.51
    Marker Score: 254
  • Cell Name: oocyte (CL0000023)
    Fold Change: 0.51
    Marker Score: 126
  • Cell Name: late promyelocyte (CL0002151)
    Fold Change: 0.5
    Marker Score: 126
  • Cell Name: enteric neuron (CL0007011)
    Fold Change: 0.5
    Marker Score: 267
  • Cell Name: transit amplifying cell of small intestine (CL0009012)
    Fold Change: 0.5
    Marker Score: 208
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: 0.5
    Marker Score: 497
  • Cell Name: erythroid progenitor cell (CL0000038)
    Fold Change: 0.5
    Marker Score: 521
  • Cell Name: pancreatic stellate cell (CL0002410)
    Fold Change: 0.5
    Marker Score: 314.5
  • Cell Name: embryonic stem cell (CL0002322)
    Fold Change: 0.49
    Marker Score: 156
  • Cell Name: exhausted T cell (CL0011025)
    Fold Change: 0.48
    Marker Score: 276
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: 0.48
    Marker Score: 578
  • Cell Name: glycinergic amacrine cell (CL4030028)
    Fold Change: 0.47
    Marker Score: 440
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.46
    Marker Score: 15762
  • Cell Name: neural cell (CL0002319)
    Fold Change: 0.45
    Marker Score: 216
  • Cell Name: intestinal crypt stem cell of small intestine (CL0009017)
    Fold Change: 0.45
    Marker Score: 303
  • Cell Name: double-positive, alpha-beta thymocyte (CL0000809)
    Fold Change: 0.45
    Marker Score: 877
  • Cell Name: kidney epithelial cell (CL0002518)
    Fold Change: 0.45
    Marker Score: 472
  • Cell Name: pre-conventional dendritic cell (CL0002010)
    Fold Change: 0.44
    Marker Score: 130
  • Cell Name: mature B cell (CL0000785)
    Fold Change: 0.44
    Marker Score: 319
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.43
    Marker Score: 197
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: 0.43
    Marker Score: 186
  • Cell Name: Sertoli cell (CL0000216)
    Fold Change: 0.42
    Marker Score: 2493
  • Cell Name: early lymphoid progenitor (CL0000936)
    Fold Change: 0.42
    Marker Score: 205
  • Cell Name: Hofbauer cell (CL3000001)
    Fold Change: 0.42
    Marker Score: 230
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: 0.41
    Marker Score: 131
  • Cell Name: common dendritic progenitor (CL0001029)
    Fold Change: 0.41
    Marker Score: 143
  • Cell Name: thymocyte (CL0000893)
    Fold Change: 0.41
    Marker Score: 432
  • Cell Name: extravillous trophoblast (CL0008036)
    Fold Change: 0.41
    Marker Score: 362
  • Cell Name: intestinal crypt stem cell of large intestine (CL0009016)
    Fold Change: 0.4
    Marker Score: 120
  • Cell Name: pro-B cell (CL0000826)
    Fold Change: 0.4
    Marker Score: 385
  • Cell Name: kidney interstitial cell (CL1000500)
    Fold Change: 0.39
    Marker Score: 280
  • Cell Name: blood cell (CL0000081)
    Fold Change: 0.39
    Marker Score: 4563
  • Cell Name: erythroid progenitor cell, mammalian (CL0001066)
    Fold Change: 0.39
    Marker Score: 125
  • Cell Name: nephron tubule epithelial cell (CL1000494)
    Fold Change: 0.39
    Marker Score: 91
  • Cell Name: skeletal muscle satellite cell (CL0000594)
    Fold Change: 0.39
    Marker Score: 259
  • Cell Name: mesodermal cell (CL0000222)
    Fold Change: 0.38
    Marker Score: 5078
  • Cell Name: GABAergic amacrine cell (CL4030027)
    Fold Change: 0.38
    Marker Score: 782
  • Cell Name: papillary tips cell (CL1000597)
    Fold Change: 0.38
    Marker Score: 77
  • Cell Name: respiratory epithelial cell (CL0002368)
    Fold Change: 0.38
    Marker Score: 210
  • Cell Name: type EC enteroendocrine cell (CL0000577)
    Fold Change: 0.38
    Marker Score: 359
  • Cell Name: mononuclear cell (CL0000842)
    Fold Change: 0.38
    Marker Score: 121
  • Cell Name: renal principal cell (CL0005009)
    Fold Change: 0.38
    Marker Score: 289
  • Cell Name: leukocyte (CL0000738)
    Fold Change: 0.37
    Marker Score: 215

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Other Information

**Key characteristics:** - It is a highly conserved gene with a predicted protein of 435 amino acids. - It is found in humans, mice, and flies. - It is expressed in a wide range of cell types, including respiratory cells, pancreatic cells, oogonial cells, and hematopoietic cells. **Pathways and functions:** - **Base excision repair:** FEN1 is a key enzyme in base excision repair pathways, which are responsible for repairing DNA damage caused by various agents, including oxidative stress, radiation, and chemicals. - **Cell cycle regulation:** It is involved in cell cycle regulation by monitoring the integrity of DNA and triggering cell cycle checkpoints when DNA damage is detected. - **DNA repair:** FEN1 plays a role in DNA repair pathways, including the repair of double-strand breaks (DSBs) and the resolution of abasic sites (ap sites) and other forms of DNA damage. - **Mitotic checkpoint:** FEN1 is also involved in the mitotic checkpoint, which prevents cell division in the presence of DNA damage. **Clinical significance:** - Mutations in the FEN1 gene have been linked to various human diseases, including cancer and developmental disorders. - Targeting FEN1 has emerged as a potential therapeutic strategy for these diseases. - Inhibitors of FEN1 have been shown to be effective in repairing DNA damage and preventing tumor growth. **Additional notes:** - The human FEN1 gene is located on chromosome 17q21. - It is a highly conserved gene, with a similar gene found in other vertebrates. - It is a well-studied gene with a well-established role in DNA repair and cellular function.

Genular Protein ID: 1186168352

Symbol: FEN1_HUMAN

Name: DNase IV

UniProtKB Accession Codes:

P39748

Database IDs:

ABCD 1 AGR 1 AlphaFoldDB 1 Antibodypedia 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 2 CORUM 1 CPTAC 5 CPTC 1 CTD 1 ChEBI 13 ChEMBL 1 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DisProt 1 DrugBank 6 ELM 1 EMBL 5 EMDB 3 EPD 1 Ensembl 1 EvolutionaryTrace 1 ExpressionAtlas 1 GO 30 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 Genevisible 1 GenomeRNAi 1 GlyGen 1 HAMAP 1 HAMAP-Rule 1 HGNC 1 HOGENOM 1 HPA 1 IDEAL 1 InParanoid 1 IntAct 1 InterPro 8 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 MaxQB 1 MetOSite 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 25 PDBsum 17 PIR 1 PRINTS 1 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 2 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 6 RefSeq 1 SAM 1 SIGNOR 1 SMART 3 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 SwissPalm 1 TopDownProteomics 1 TreeFam 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 8007985

Title: Structural and functional conservation of the human homolog of the Schizosaccharomyces pombe rad2 gene, which is required for chromosome segregation and recovery from DNA damage.

PubMed ID: 8007985

DOI: 10.1128/mcb.14.7.4878-4888.1994

PubMed ID: 7774922

Title: Sequence of human FEN-1, a structure-specific endonuclease, and chromosomal localization of the gene (FEN1) in mouse and human.

PubMed ID: 7774922

DOI: 10.1016/0888-7543(95)80129-a

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 7961795

Title: Structural and functional homology between mammalian DNase IV and the 5'-nuclease domain of Escherichia coli DNA polymerase I.

PubMed ID: 7961795

DOI: 10.1016/s0021-9258(19)61935-6

PubMed ID: 8621570

Title: Essential amino acids for substrate binding and catalysis of human flap endonuclease 1.

PubMed ID: 8621570

DOI: 10.1074/jbc.271.16.9173

PubMed ID: 9305916

Title: The DNA repair endonuclease XPG binds to proliferating cell nuclear antigen (PCNA) and shares sequence elements with the PCNA-binding regions of FEN-1 and cyclin-dependent kinase inhibitor p21.

PubMed ID: 9305916

DOI: 10.1074/jbc.272.39.24522

PubMed ID: 10744741

Title: Mechanism whereby proliferating cell nuclear antigen stimulates flap endonuclease 1.

PubMed ID: 10744741

DOI: 10.1074/jbc.275.14.10498

PubMed ID: 11430825

Title: Regulation of human flap endonuclease-1 activity by acetylation through the transcriptional coactivator p300.

PubMed ID: 11430825

DOI: 10.1016/s1097-2765(01)00272-6

PubMed ID: 11986308

Title: Arginine residues 47 and 70 of human flap endonuclease-1 are involved in DNA substrate interactions and cleavage site determination.

PubMed ID: 11986308

DOI: 10.1074/jbc.m111941200

PubMed ID: 18499658

Title: Studies with the human cohesin establishment factor, ChlR1. Association of ChlR1 with Ctf18-RFC and Fen1.

PubMed ID: 18499658

DOI: 10.1074/jbc.m802696200

PubMed ID: 18443037

Title: Nucleolar localization and dynamic roles of flap endonuclease 1 in ribosomal DNA replication and damage repair.

PubMed ID: 18443037

DOI: 10.1128/mcb.00200-08

PubMed ID: 19336415

Title: Human DNA polymerase beta polymorphism, Arg137Gln, impairs its polymerase activity and interaction with PCNA and the cellular base excision repair capacity.

PubMed ID: 19336415

DOI: 10.1093/nar/gkp201

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 20729856

Title: Methylation of FEN1 suppresses nearby phosphorylation and facilitates PCNA binding.

PubMed ID: 20729856

DOI: 10.1038/nchembio.422

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 22814378

Title: N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB.

PubMed ID: 22814378

DOI: 10.1073/pnas.1210303109

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 23675412

Title: A cryptic targeting signal creates a mitochondrial FEN1 isoform with tailed R-loop binding properties.

PubMed ID: 23675412

DOI: 10.1371/journal.pone.0062340

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 26760506

Title: R152C DNA Pol beta mutation impairs base excision repair and induces cellular transformation.

PubMed ID: 26760506

DOI: 10.18632/oncotarget.6849

PubMed ID: 26751069

Title: Wuho is a new member in maintaining genome stability through its interaction with flap endonuclease 1.

PubMed ID: 26751069

DOI: 10.1371/journal.pbio.1002349

PubMed ID: 15576034

Title: Structural and thermodynamic analysis of human PCNA with peptides derived from DNA polymerase-delta p66 subunit and flap endonuclease-1.

PubMed ID: 15576034

DOI: 10.1016/j.str.2004.09.018

PubMed ID: 15616578

Title: Structural basis for recruitment of human flap endonuclease 1 to PCNA.

PubMed ID: 15616578

DOI: 10.1038/sj.emboj.7600519

Sequence Information:

  • Length: 380
  • Mass: 42593
  • Checksum: 5154F2F6E57592C5
  • Sequence:
    • MGIQGLAKLI ADVAPSAIRE NDIKSYFGRK VAIDASMSIY QFLIAVRQGG DVLQNEEGET 
TSHLMGMFYR TIRMMENGIK PVYVFDGKPP QLKSGELAKR SERRAEAEKQ LQQAQAAGAE 
QEVEKFTKRL VKVTKQHNDE CKHLLSLMGI PYLDAPSEAE ASCAALVKAG KVYAAATEDM 
DCLTFGSPVL MRHLTASEAK KLPIQEFHLS RILQELGLNQ EQFVDLCILL GSDYCESIRG 
IGPKRAVDLI QKHKSIEEIV RRLDPNKYPV PENWLHKEAH QLFLEPEVLD PESVELKWSE 
PNEEELIKFM CGEKQFSEER IRSGVKRLSK SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP 
KGSTKKKAKT GAAGKFKRGK

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. For the full schema, download it here.