Details for: P2RY11

Gene ID: 5032

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: P2RY11

Ensembl ID: ENSG00000244165

Description: purinergic receptor P2Y11

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • CD8-positive, alpha-beta memory T cell, CD45RO-positive CL0001203
    CSI 9.37
    rCSI 11.35%
    PRS 88.44
  • cytotoxic T cell CL0000910
    CSI 2.93
    rCSI 16.77%
    PRS 96.16
  • ependymal cell CL0000065
    CSI 2.86
    rCSI 5.81%
    PRS 92.28
  • innate lymphoid cell CL0001065
    CSI 2.05
    rCSI 4.23%
    PRS 94.81
  • basal cell of epidermis CL0002187
    CSI 1.48
    rCSI 2.62%
    PRS 84.05
  • helper T cell CL0000912
    CSI 1.41
    rCSI 2%
    PRS 95.28
  • melanocyte of skin CL1000458
    CSI 1.22
    rCSI 1.67%
    PRS 85.17

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

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  • Node Color (Target Cell CSI, relative to current network):
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    • High
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    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [P2RY11](/details-gene/5032) is a protein-coding gene that encodes the purinergic receptor P2Y11, a member of the G protein-coupled receptor (GPCR) family. This receptor is uniquely characterized by its dual coupling to both Gs and Gq signaling pathways, allowing it to activate both adenylate cyclase and phospholipase C in response to extracellular ATP ([Link](https://pubmed.ncbi.nlm.nih.gov/9405388/)). Functionally, [P2RY11](/details-gene/5032) is involved in [calcium-mediated signaling](/details-ontology/GO:0019722) and the cellular response to ATP ([GO:0071318](https://www.ebi.ac.uk/QuickGO/term/GO:0071318)). Expression data highlights its significant role within the immune system, where it shows exceptionally high significance in [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), suggesting a key function in adaptive immune memory. ## Cellular Roles and Expression Landscape The **Overall** expression profile of [P2RY11](/details-gene/5032) points to a specialized role in distinct cell populations, with a strong predilection for the immune system. The gene exhibits its highest significance by a substantial margin in [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203) (CSI: 9.37), indicating it may be a critical modulator of long-term immune surveillance and memory. Its role in immunity is further supported by significant expression in other key lymphocyte populations, including [cytotoxic T cell](/details-cell/CL0000910) and [innate lymphoid cell](/details-cell/CL0001065). This pattern suggests [P2RY11](/details-gene/5032) is broadly involved in the functional responses of both the adaptive and innate immune arms, likely mediating cellular reactions to ATP released in environments of inflammation or tissue damage. Beyond the immune context, [P2RY11](/details-gene/5032) also shows notable significance in non-hematopoietic cell types such as [ependymal cell](/details-cell/CL0000065), [basal cell of epidermis](/details-cell/CL0002187), and [melanocyte of skin](/details-cell/CL1000458). This suggests that its function as an ATP sensor is not restricted to immunity and may extend to neuronal signal transduction and epithelial tissue homeostasis. ## Pathways and Molecular Function [P2RY11](/details-gene/5032) functions as a receptor for extracellular nucleotides, primarily ATP, and is an integral component of the plasma membrane ([GO:0005886](https://www.ebi.ac.uk/QuickGO/term/GO:0005886)). As a member of the [P2Y receptors](/details-pathway/R-HSA-417957) family, it participates in GPCR-mediated signal transduction. Its primary molecular function is [G protein-coupled ATP receptor activity](/details-ontology/GO:0045031). Upon ligand binding, [P2RY11](/details-gene/5032) initiates downstream signaling cascades through two distinct G protein families. It activates [G alpha (s) signalling events](/details-pathway/R-HSA-418555), leading to the [activation of adenylate cyclase activity](/details-ontology/GO:0007190) and an increase in intracellular cAMP. Concurrently, it engages in [G alpha (q) signalling events](/details-pathway/R-HSA-416476), which triggers the [phospholipase c-activating g protein-coupled receptor signaling pathway](/details-ontology/GO:0007200) and results in calcium mobilization. This dual signaling capacity allows [P2RY11](/details-gene/5032) to orchestrate complex cellular responses, integrating different downstream effectors to fine-tune processes such as proliferation, differentiation, and apoptosis, particularly within the T cell lineages where it is prominently expressed. ## Research Directions The unique expression pattern and dual signaling capability of [P2RY11](/details-gene/5032) suggest it is a key regulator of cellular function, particularly in the context of immune memory. Further research is warranted to elucidate its precise roles in both health and disease. **Proposed Hypotheses:** 1. Given its exceptionally high significance in [CD8-positive, alpha-beta memory T cell, CD45RO-positive](/details-cell/CL0001203), signaling through [P2RY11](/details-gene/5032) in response to extracellular ATP is essential for the survival, maintenance, and/or rapid reactivation of these cells upon secondary antigen exposure. 2. The notable expression of [P2RY11](/details-gene/5032) in [ependymal cell](/details-cell/CL0000065) suggests it plays a role in central nervous system homeostasis. It may function as a sensor for ATP in the cerebrospinal fluid, regulating ciliary beat frequency or ion transport to maintain fluid balance. **Experimental Approach:** To test the first hypothesis regarding the role of [P2RY11](/details-gene/5032) in memory T cell function, primary human CD8+ memory T cells could be isolated and treated with a specific P2RY11 agonist (e.g., ATP-gamma-S) or antagonist. The functional consequences could be measured by assessing key cellular outcomes such as proliferation (via CFSE dilution), survival following cytokine withdrawal (via Annexin V staining), and effector function (cytokine production like IFN-gamma and TNF-alpha measured by ELISA or intracellular staining) following T-cell receptor stimulation. A complementary approach would involve CRISPR-Cas9-mediated knockout of [P2RY11](/details-gene/5032) in these cells to confirm the specificity of the observed effects. **Therapeutic Potential:** As a cell surface GPCR with highly specific expression on immune cell subsets, [P2RY11](/details-gene/5032) is a promising therapeutic target. In autoimmune diseases or organ transplant rejection, where memory T cells drive pathology, a P2RY11 antagonist could be used to suppress their activity and survival. Conversely, in the context of cancer immunotherapy, a P2RY11 agonist could potentially be used to enhance the persistence and effector function of anti-tumor cytotoxic and memory T cells, making it a candidate for combination therapies to improve the efficacy of checkpoint inhibitors or CAR-T cell treatments.

Genular Protein ID: 3261044797

Symbol: P2Y11_HUMAN

Name: P2Y purinoceptor 11

UniProtKB Accession Codes:

Q96G91 B2R8X9 O43190 Q9BYU4 Q9H170

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChEMBL 1 DMDM 1 DNASU 1 DisGeNET 1 DrugBank 2 DrugCentral 1 EMBL 8 Ensembl 1 GO 11 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyCosmos 1 GlyGen 1 GuidetoPHARMACOLOGY 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 3 KEGG 1 MANE-Select 1 MIM 1 MalaCards 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 Orphanet 1 OrthoDB 1 PANTHER 2 PRINTS 2 PRO 1 PROSITE 2 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 RNAct 1 Reactome 3 RefSeq 1 SIGNOR 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 iPTMnet 1 neXtProt 1

Citations:

PubMed ID: 11278528

Title: Cotranscription and intergenic splicing of human P2Y11 and SSF1 genes.

PubMed ID: 11278528

DOI: 10.1074/jbc.m009609200

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9405388

Title: Cloning of a human purinergic P2Y receptor coupled to phospholipase C and adenylyl cyclase.

PubMed ID: 9405388

DOI: 10.1074/jbc.272.51.31969

Sequence Information:

  • Length: 374
  • Mass: 40345
  • Checksum: C02DF9B3B381D25E
  • Sequence:
    • MAANVSGAKS CPANFLAAAD DKLSGFQGDF LWPILVVEFL VAVASNGLAL YRFSIRKQRP 
WHPAVVFSVQ LAVSDLLCAL TLPPLAAYLY PPKHWRYGEA ACRLERFLFT CNLLGSVIFI 
TCISLNRYLG IVHPFFARSH LRPKHAWAVS AAGWVLAALL AMPTLSFSHL KRPQQGAGNC 
SVARPEACIK CLGTADHGLA AYRAYSLVLA GLGCGLPLLL TLAAYGALGR AVLRSPGMTV 
AEKLRVAALV ASGVALYASS YVPYHIMRVL NVDARRRWST RCPSFADIAQ ATAALELGPY 
VGYQVMRGLM PLAFCVHPLL YMAAVPSLGC CCRHCPGYRD SWNPEDAKST GQALPLNATA 
APKPSEPQSR ELSQ