Details for: RFX3

Gene ID: 5991

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: RFX3

Ensembl ID: ENSG00000080298

Description: regulatory factor X3

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

  • Cell maturation
    (GO:0048469)
  • Chromatin
    (GO:0000785)
  • Cilium-dependent cell motility
    (GO:0060285)
  • Cilium assembly
    (GO:0060271)
  • Dna-binding transcription factor activity
    (GO:0003700)
  • Dna-binding transcription factor activity, rna polymerase ii-specific
    (GO:0000981)
  • Dna-templated transcription
    (GO:0006351)
  • Dna binding
    (GO:0003677)
  • Endocrine pancreas development
    (GO:0031018)
  • Epithelial cilium movement involved in determination of left/right asymmetry
    (GO:0060287)
  • Extracellular region
    (GO:0005576)
  • Negative regulation of dna-templated transcription
    (GO:0045892)
  • Nucleus
    (GO:0005634)
  • Positive regulation of dna-templated transcription
    (GO:0045893)
  • Positive regulation of transcription by rna polymerase ii
    (GO:0045944)
  • Positive regulation of type b pancreatic cell development
    (GO:2000078)
  • Protein binding
    (GO:0005515)
  • Regulation of dna-templated transcription
    (GO:0006355)
  • Regulation of insulin secretion
    (GO:0050796)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)
  • Sequence-specific double-stranded dna binding
    (GO:1990837)
  • Transcription cis-regulatory region binding
    (GO:0000976)
  • Transcription regulator complex
    (GO:0005667)
  • Type b pancreatic cell maturation
    (GO:0072560)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • choroid plexus epithelial cell CL0000706
    CSI 56.64
    rCSI 92.76%
    PRS 32.12
  • ependymal cell CL0000065
    CSI 44.87
    rCSI 91.06%
    PRS 24.71
  • Bergmann glial cell CL0000644
    CSI 40.37
    rCSI 55.23%
    PRS 37.6
  • naive thymus-derived CD8-positive, alpha-beta T cell CL0000900
    CSI 38.82
    rCSI 27.26%
    PRS 63.56
  • sncg GABAergic cortical interneuron CL4023015
    CSI 33.77
    rCSI 54.31%
    PRS 28.36
  • L2/3 intratelencephalic projecting glutamatergic neuron CL4030059
    CSI 33.66
    rCSI 73.02%
    PRS 31.57
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 33.55
    rCSI 41.73%
    PRS 24.98
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 31.8
    rCSI 53.38%
    PRS 26.37
  • VIP GABAergic cortical interneuron CL4023016
    CSI 31.1
    rCSI 37.14%
    PRS 26.28
  • blood vessel endothelial cell CL0000071
    CSI 31.04
    rCSI 64.4%
    PRS 38.74
  • sst GABAergic cortical interneuron CL4023017
    CSI 28.47
    rCSI 36.7%
    PRS 27.25
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 27.07
    rCSI 65.79%
    PRS 25.6
  • astrocyte of the cerebral cortex CL0002605
    CSI 25.74
    rCSI 57.72%
    PRS 26.97
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 24.42
    rCSI 43.12%
    PRS 25.45
  • astrocyte CL0000127
    CSI 22.81
    rCSI 48.59%
    PRS 21.45
  • naive B cell CL0000788
    CSI 21.81
    rCSI 18.71%
    PRS 49.78
  • lung ciliated cell CL1000271
    CSI 21.56
    rCSI 24.93%
    PRS 31.44
  • ciliated epithelial cell CL0000067
    CSI 20.35
    rCSI 17.89%
    PRS 30.69
  • cerebral cortex endothelial cell CL1001602
    CSI 19.45
    rCSI 33.64%
    PRS 32.01
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 19.31
    rCSI 69.5%
    PRS 25.31
  • innate lymphoid cell CL0001065
    CSI 19.26
    rCSI 39.76%
    PRS 47.53
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 18.1
    rCSI 20.9%
    PRS 35.44
  • macroglial cell CL0000126
    CSI 18
    rCSI 46.27%
    PRS 43.69
  • mature B cell CL0000785
    CSI 17.61
    rCSI 15.31%
    PRS 48.94
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 17.44
    rCSI 54.54%
    PRS 29.52
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 17.43
    rCSI 65.87%
    PRS 27.24
  • L6b glutamatergic cortical neuron CL4023038
    CSI 16.24
    rCSI 50.77%
    PRS 27.45
  • L4 intratelencephalic projecting glutamatergic neuron CL4030063
    CSI 15.63
    rCSI 37.37%
    PRS 30.84
  • neuron CL0000540
    CSI 15
    rCSI 39.93%
    PRS 33.65
  • erythrocyte CL0000232
    CSI 14.64
    rCSI 33.22%
    PRS 46.19
  • stem cell CL0000034
    CSI 14.64
    rCSI 14.12%
    PRS 31.88
  • oligodendrocyte precursor cell CL0002453
    CSI 14.32
    rCSI 31.52%
    PRS 27.9
  • kidney interstitial alternatively activated macrophage CL1000695
    CSI 13.58
    rCSI 35.4%
    PRS 39.5
  • precursor B cell CL0000817
    CSI 12.73
    rCSI 11.15%
    PRS 49.84
  • glioblast CL0000030
    CSI 12.27
    rCSI 19.58%
    PRS 34.94
  • intermediate monocyte CL0002393
    CSI 12
    rCSI 18.1%
    PRS 42.11
  • neural cell CL0002319
    CSI 11.83
    rCSI 44.63%
    PRS 35.53
  • retinal ganglion cell CL0000740
    CSI 11.79
    rCSI 26.04%
    PRS 30.03
  • fallopian tube secretory epithelial cell CL4030006
    CSI 11.7
    rCSI 11.26%
    PRS 41.08
  • erythroblast CL0000765
    CSI 11.6
    rCSI 30.77%
    PRS 53.87
  • hepatic stellate cell CL0000632
    CSI 11.43
    rCSI 42.83%
    PRS 34.46
  • radial glial cell CL0000681
    CSI 11.4
    rCSI 15.84%
    PRS 40.15
  • interneuron CL0000099
    CSI 11.36
    rCSI 22.8%
    PRS 31.28
  • neural crest cell CL0011012
    CSI 11.03
    rCSI 8.72%
    PRS 29.33
  • ciliated cell CL0000064
    CSI 11
    rCSI 17.83%
    PRS 39.23
  • enteroendocrine cell of small intestine CL0009006
    CSI 10.8
    rCSI 23.79%
    PRS 54.94
  • effector memory CD8-positive, alpha-beta T cell CL0000913
    CSI 10.77
    rCSI 9.81%
    PRS 54.91
  • adipocyte CL0000136
    CSI 10.72
    rCSI 13.76%
    PRS 36.83
  • melanocyte CL0000148
    CSI 10.64
    rCSI 7.88%
    PRS 34.79
  • neutrophil CL0000775
    CSI 10.57
    rCSI 59.14%
    PRS 53.86
  • retinal blood vessel endothelial cell CL0002585
    CSI 10.56
    rCSI 16.86%
    PRS 43.76
  • diffuse bipolar 6 cell CL4033032
    CSI 10.48
    rCSI 55.12%
    PRS 38.97
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 10.47
    rCSI 61.63%
    PRS 27.6
  • enteroendocrine cell CL0000164
    CSI 10.26
    rCSI 14.02%
    PRS 43.13
  • epithelial cell of proximal tubule CL0002306
    CSI 10.16
    rCSI 24.82%
    PRS 37.1
  • ciliated columnar cell of tracheobronchial tree CL0002145
    CSI 10.01
    rCSI 22.81%
    PRS 39.42
  • L5/6 near-projecting glutamatergic neuron CL4030067
    CSI 10
    rCSI 32.86%
    PRS 30.26
  • kidney loop of Henle thin descending limb epithelial cell CL1001111
    CSI 9.85
    rCSI 13.97%
    PRS 37.76
  • vascular leptomeningeal cell CL4023051
    CSI 9.69
    rCSI 17%
    PRS 33.53
  • multi-ciliated epithelial cell CL0005012
    CSI 9.67
    rCSI 9.65%
    PRS 35.18
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 9.24
    rCSI 11.86%
    PRS 38.57
  • cerebral cortex neuron CL0010012
    CSI 9.19
    rCSI 37.43%
    PRS 38.75
  • inhibitory interneuron CL0000498
    CSI 9.01
    rCSI 20.8%
    PRS 33.23
  • glutamatergic neuron CL0000679
    CSI 8.96
    rCSI 18.41%
    PRS 36.12
  • differentiation-committed oligodendrocyte precursor CL4023059
    CSI 8.91
    rCSI 16.19%
    PRS 34.85
  • retinal cone cell CL0000573
    CSI 8.88
    rCSI 14.29%
    PRS 32.15
  • chondrocyte CL0000138
    CSI 8.87
    rCSI 14.1%
    PRS 34.41
  • Kupffer cell CL0000091
    CSI 8.72
    rCSI 19.95%
    PRS 39.91
  • central nervous system neuron CL2000029
    CSI 8.44
    rCSI 62%
    PRS 29.56
  • cerebellar granule cell CL0001031
    CSI 8.3
    rCSI 12.2%
    PRS 37.1
  • cardiac neuron CL0010022
    CSI 8.11
    rCSI 25.94%
    PRS 37.62
  • neuroendocrine cell CL0000165
    CSI 8.08
    rCSI 31.23%
    PRS 59.48
  • fibroblast of lung CL0002553
    CSI 7.97
    rCSI 7.42%
    PRS 40.32
  • peripheral nervous system neuron CL2000032
    CSI 7.84
    rCSI 10.69%
    PRS 34.75
  • mononuclear phagocyte CL0000113
    CSI 7.66
    rCSI 16.86%
    PRS 44.2
  • dopaminergic neuron CL0000700
    CSI 7.58
    rCSI 42.85%
    PRS 28.07
  • lung secretory cell CL1000272
    CSI 7.56
    rCSI 18.72%
    PRS 38.62
  • alveolar macrophage CL0000583
    CSI 7.51
    rCSI 12.37%
    PRS 45.69
  • retina horizontal cell CL0000745
    CSI 7.42
    rCSI 11.31%
    PRS 37.46
  • retinal rod cell CL0000604
    CSI 7.37
    rCSI 12.99%
    PRS 39.32
  • renal interstitial pericyte CL1001318
    CSI 7.26
    rCSI 20.01%
    PRS 37.61
  • conjunctival epithelial cell CL1000432
    CSI 7.22
    rCSI 11.03%
    PRS 41
  • alveolar type 1 fibroblast cell CL4028004
    CSI 6.95
    rCSI 7.61%
    PRS 44.23
  • fibroblast of cardiac tissue CL0002548
    CSI 6.92
    rCSI 33.13%
    PRS 38.87
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 6.9
    rCSI 7.04%
    PRS 52.83
  • retinal bipolar neuron CL0000748
    CSI 6.69
    rCSI 12.53%
    PRS 31.27
  • central nervous system macrophage CL0000878
    CSI 6.68
    rCSI 22.14%
    PRS 41.71
  • pulmonary alveolar type 1 cell CL0002062
    CSI 6.57
    rCSI 37.9%
    PRS 43.15
  • basal cell CL0000646
    CSI 6.56
    rCSI 8.77%
    PRS 42.16
  • BEST4+ enteroycte CL4030026
    CSI 6.54
    rCSI 8.13%
    PRS 43.01
  • pro-B cell CL0000826
    CSI 6.49
    rCSI 5.38%
    PRS 41.3
  • Schwann cell CL0002573
    CSI 6.33
    rCSI 18%
    PRS 40.64
  • rod bipolar cell CL0000751
    CSI 6.24
    rCSI 11.21%
    PRS 34.56
  • diffuse bipolar 3b cell CL4033030
    CSI 6.14
    rCSI 40.78%
    PRS 39.57
  • intestinal tuft cell CL0019032
    CSI 6.14
    rCSI 9.39%
    PRS 44.82
  • kidney loop of Henle thin ascending limb epithelial cell CL1001107
    CSI 6.13
    rCSI 15.85%
    PRS 37.01
  • epithelial cell of lower respiratory tract CL0002632
    CSI 6.07
    rCSI 4.7%
    PRS 40.64
  • lung pericyte CL0009089
    CSI 5.96
    rCSI 15.74%
    PRS 47.49
  • H2 horizontal cell CL0004218
    CSI 5.96
    rCSI 29.61%
    PRS 40.68
  • pulmonary alveolar type 2 cell CL0002063
    CSI 5.89
    rCSI 9.13%
    PRS 50.61
  • pancreatic ductal cell CL0002079
    CSI 0.3
    rCSI 0.6%
    PRS 42.3%
  • P/D1 enteroendocrine cell CL0002268
    CSI 0.6
    rCSI 3.1%
    PRS 63.7%
  • mesenchymal cell CL0008019
    CSI 0.7
    rCSI 1.7%
    PRS 37.2%
  • type EC enteroendocrine cell CL0000577
    CSI 0.8
    rCSI 2.8%
    PRS 53.8%
  • H1 horizontal cell CL0004217
    CSI 1.1
    rCSI 4.2%
    PRS 43.6%
  • deuterosomal cell CL4033044
    CSI 1.1
    rCSI 3.7%
    PRS 50.7%
  • cerebral cortex pyramidal neuron CL4023111
    CSI 1.1
    rCSI 6.9%
    PRS 63.4%
  • type L enteroendocrine cell CL0002279
    CSI 1.1
    rCSI 2.1%
    PRS 60.5%
  • mesodermal cell CL0000222
    CSI 1.2
    rCSI 1.4%
    PRS 38.7%
  • regular ventricular cardiac myocyte CL0002131
    CSI 1.3
    rCSI 8.0%
    PRS 33.7%
  • luminal epithelial cell of mammary gland CL0002326
    CSI 1.4
    rCSI 2.5%
    PRS 56.3%
  • S cone cell CL0003050
    CSI 1.5
    rCSI 6.4%
    PRS 38.1%
  • extravillous trophoblast CL0008036
    CSI 1.5
    rCSI 1.8%
    PRS 36.6%
  • tuft cell of colon CL0009041
    CSI 1.5
    rCSI 3.4%
    PRS 59.0%
  • blood vessel smooth muscle cell CL0019018
    CSI 1.5
    rCSI 12.0%
    PRS 35.9%
  • corneal epithelial cell CL0000575
    CSI 1.5
    rCSI 4.3%
    PRS 58.0%
  • kidney distal convoluted tubule epithelial cell CL1000849
    CSI 1.5
    rCSI 15.8%
    PRS 42.5%
  • mesangial cell CL0000650
    CSI 1.5
    rCSI 6.2%
    PRS 52.4%
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 1.5
    rCSI 6.0%
    PRS 60.2%
  • stromal cell CL0000499
    CSI 1.6
    rCSI 4.6%
    PRS 41.7%
  • regular atrial cardiac myocyte CL0002129
    CSI 1.6
    rCSI 5.3%
    PRS 40.0%
  • keratocyte CL0002363
    CSI 1.7
    rCSI 4.0%
    PRS 50.8%
  • IgG plasma cell CL0000985
    CSI 1.8
    rCSI 2.2%
    PRS 59.2%
  • retinal pigment epithelial cell CL0002586
    CSI 1.8
    rCSI 3.6%
    PRS 40.2%
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 1.9
    rCSI 1.4%
    PRS 39.0%
  • GABAergic interneuron CL0011005
    CSI 1.9
    rCSI 29.6%
    PRS 43.8%
  • Hofbauer cell CL3000001
    CSI 1.9
    rCSI 3.6%
    PRS 50.0%
  • endothelial cell of arteriole CL1000412
    CSI 1.9
    rCSI 10.6%
    PRS 64.9%
  • diffuse bipolar 4 cell CL4033031
    CSI 1.9
    rCSI 22.2%
    PRS 36.0%
  • amacrine cell CL0000561
    CSI 2.0
    rCSI 5.7%
    PRS 32.8%
  • neural progenitor cell CL0011020
    CSI 2.1
    rCSI 9.2%
    PRS 35.0%
  • airway submucosal gland duct basal cell CL4033024
    CSI 2.2
    rCSI 13.8%
    PRS 59.9%
  • cardiac blood vessel endothelial cell CL0010006
    CSI 2.3
    rCSI 16.0%
    PRS 35.7%
  • neuroplacodal cell CL0000032
    CSI 2.3
    rCSI 21.0%
    PRS 60.3%
  • starburst amacrine cell CL0004232
    CSI 2.3
    rCSI 19.2%
    PRS 37.3%
  • respiratory suprabasal cell CL4033048
    CSI 2.3
    rCSI 3.0%
    PRS 45.0%
  • ON parasol ganglion cell CL4033052
    CSI 2.3
    rCSI 33.2%
    PRS 33.7%
  • lung neuroendocrine cell CL1000223
    CSI 2.4
    rCSI 3.5%
    PRS 45.1%
  • type B pancreatic cell CL0000169
    CSI 2.4
    rCSI 5.3%
    PRS 37.8%
  • midbrain dopaminergic neuron CL2000097
    CSI 2.7
    rCSI 17.3%
    PRS 47.6%
  • OFF midget ganglion cell CL4033047
    CSI 2.7
    rCSI 55.1%
    PRS 35.1%
  • podocyte CL0000653
    CSI 2.8
    rCSI 12.3%
    PRS 39.0%
  • enteroglial cell CL4040002
    CSI 2.8
    rCSI 14.5%
    PRS 50.0%
  • serotonergic neuron CL0000850
    CSI 2.8
    rCSI 12.3%
    PRS 28.0%
  • tissue-resident macrophage CL0000864
    CSI 2.8
    rCSI 13.2%
    PRS 60.4%
  • pancreatic D cell CL0000173
    CSI 2.9
    rCSI 2.8%
    PRS 42.7%
  • indirect pathway medium spiny neuron CL4023029
    CSI 2.9
    rCSI 70.1%
    PRS 26.7%
  • direct pathway medium spiny neuron CL4023026
    CSI 2.9
    rCSI 70.3%
    PRS 25.8%
  • ON midget ganglion cell CL4033046
    CSI 3.1
    rCSI 62.0%
    PRS 33.8%
  • endothelial cell of pericentral hepatic sinusoid CL0019022
    CSI 3.1
    rCSI 9.7%
    PRS 52.6%
  • cardiac muscle cell CL0000746
    CSI 3.1
    rCSI 4.5%
    PRS 32.8%
  • double negative thymocyte CL0002489
    CSI 3.2
    rCSI 2.2%
    PRS 48.4%
  • squamous epithelial cell CL0000076
    CSI 3.2
    rCSI 7.7%
    PRS 46.2%
  • myoepithelial cell CL0000185
    CSI 3.3
    rCSI 8.3%
    PRS 48.3%
  • plasmablast CL0000980
    CSI 3.3
    rCSI 2.6%
    PRS 46.5%
  • OFFx cell CL4033036
    CSI 3.3
    rCSI 15.6%
    PRS 38.9%
  • glycinergic amacrine cell CL4030028
    CSI 3.3
    rCSI 8.7%
    PRS 40.4%
  • flat midget bipolar cell CL4033033
    CSI 3.4
    rCSI 24.5%
    PRS 37.0%
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 3.4
    rCSI 8.3%
    PRS 57.7%
  • skeletal muscle satellite stem cell CL0008011
    CSI 3.5
    rCSI 15.4%
    PRS 65.6%
  • glial cell CL0000125
    CSI 3.6
    rCSI 13.8%
    PRS 35.0%
  • CD14-positive monocyte CL0001054
    CSI 3.6
    rCSI 4.5%
    PRS 51.4%
  • small intestine goblet cell CL1000495
    CSI 3.7
    rCSI 8.1%
    PRS 50.3%
  • mature astrocyte CL0002627
    CSI 3.7
    rCSI 15.8%
    PRS 38.6%
  • endocardial cell CL0002350
    CSI 3.8
    rCSI 18.0%
    PRS 42.4%
  • mesothelial cell CL0000077
    CSI 3.8
    rCSI 14.8%
    PRS 19.1%
  • tracheobronchial smooth muscle cell CL0019019
    CSI 3.8
    rCSI 6.7%
    PRS 48.7%
  • pulmonary artery endothelial cell CL1001568
    CSI 3.8
    rCSI 5.2%
    PRS 53.0%
  • intestinal crypt stem cell of small intestine CL0009017
    CSI 3.9
    rCSI 10.4%
    PRS 49.2%
  • granulocyte CL0000094
    CSI 4.0
    rCSI 6.1%
    PRS 49.1%
  • enteroendocrine cell of colon CL0009042
    CSI 4.0
    rCSI 18.8%
    PRS 66.9%
  • cerebellar neuron CL1001611
    CSI 4.1
    rCSI 35.9%
    PRS 29.9%
  • endothelial cell of placenta CL0009092
    CSI 4.1
    rCSI 20.1%
    PRS 51.6%
  • regulatory T cell CL0000815
    CSI 4.2
    rCSI 4.8%
    PRS 63.6%
  • brush cell of tracheobronchial tree CL0002075
    CSI 4.2
    rCSI 12.4%
    PRS 51.9%
  • basophil CL0000767
    CSI 4.3
    rCSI 9.1%
    PRS 61.6%
  • parietal epithelial cell CL1000452
    CSI 4.3
    rCSI 11.5%
    PRS 34.2%
  • diffuse bipolar 1 cell CL4033027
    CSI 4.3
    rCSI 32.3%
    PRS 37.1%
  • pancreatic A cell CL0000171
    CSI 4.3
    rCSI 4.5%
    PRS 42.9%
  • mature microglial cell CL0002629
    CSI 4.4
    rCSI 18.2%
    PRS 41.8%
  • renal beta-intercalated cell CL0002201
    CSI 4.5
    rCSI 10.7%
    PRS 43.0%
  • renal principal cell CL0005009
    CSI 4.5
    rCSI 11.7%
    PRS 45.9%
  • kidney loop of Henle thick ascending limb epithelial cell CL1001106
    CSI 4.6
    rCSI 39.7%
    PRS 43.0%
  • respiratory basal cell CL0002633
    CSI 4.8
    rCSI 4.9%
    PRS 46.1%
  • GABAergic neuron CL0000617
    CSI 4.8
    rCSI 16.0%
    PRS 30.2%
  • cardiac endothelial cell CL0010008
    CSI 4.8
    rCSI 19.3%
    PRS 39.1%
  • Mueller cell CL0000636
    CSI 4.9
    rCSI 11.2%
    PRS 34.7%
  • microglial cell CL0000129
    CSI 4.9
    rCSI 19.7%
    PRS 52.0%
  • medium spiny neuron CL1001474
    CSI 4.9
    rCSI 42.3%
    PRS 28.9%
  • endothelial cell of vascular tree CL0002139
    CSI 5.0
    rCSI 27.4%
    PRS 44.7%
  • forebrain radial glial cell CL0013000
    CSI 5.1
    rCSI 16.2%
    PRS 48.6%
  • diffuse bipolar 2 cell CL4033028
    CSI 5.1
    rCSI 39.3%
    PRS 38.8%
  • GABAergic amacrine cell CL4030027
    CSI 5.1
    rCSI 17.4%
    PRS 33.4%
  • common dendritic progenitor CL0001029
    CSI 5.1
    rCSI 6.4%
    PRS 49.6%
  • invaginating midget bipolar cell CL4033034
    CSI 5.1
    rCSI 30.3%
    PRS 39.0%
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 5.3
    rCSI 4.8%
    PRS 37.2%
  • diffuse bipolar 3a cell CL4033029
    CSI 5.4
    rCSI 36.6%
    PRS 38.6%
  • kidney connecting tubule epithelial cell CL1000768
    CSI 5.4
    rCSI 13.7%
    PRS 32.0%
  • mature T cell CL0002419
    CSI 5.4
    rCSI 4.2%
    PRS 56.5%
  • hepatocyte CL0000182
    CSI 5.5
    rCSI 9.8%
    PRS 39.0%

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [RFX3](/details-gene/5991) is a protein-coding gene that encodes Regulatory Factor X3, a member of the RFX family of DNA-binding transcription factors. These proteins are characterized by a highly conserved winged-helix DNA-binding domain. Functionally, [RFX3](/details-gene/5991) is a key regulator of gene expression involved in fundamental biological processes, most notably [cilium assembly](/details-cell/GO:0060271) and function, which is reflected in its high expression in specialized ciliated cells such as [choroid plexus epithelial cells](/details-cell/CL0000706) and [ependymal cells](/details-cell/CL0000065). Additionally, it plays significant roles in endocrine pancreas development and the regulation of specific immune cell populations. Its clinical relevance is noted in OMIM under entry `[601337](https://omim.org/entry/601337)`. ## Cellular Roles and Expression Landscape The expression profile of [RFX3](/details-gene/5991) suggests a highly specialized function, particularly within the central nervous system (CNS) and specific immune compartments. **Overall**, the gene shows the highest significance in CNS-associated cells that are known to be ciliated. It is a top marker for [choroid plexus epithelial cell](/details-cell/CL0000706) (CSI: 56.64) and [ependymal cell](/details-cell/CL0000065) (CSI: 44.87), which line the ventricles of the brain and are critical for cerebrospinal fluid circulation. Its high significance in [Bergmann glial cells](/details-cell/CL0000644) (CSI: 40.37) further underscores its importance in specialized glial populations. Beyond these ciliated cell types, [RFX3](/details-gene/5991) is also significantly expressed across a diverse range of neuronal subtypes, including multiple GABAergic interneurons (e.g., [sncg GABAergic cortical interneuron](/details-cell/CL4023015), [pvalb GABAergic cortical interneuron](/details-cell/CL4023018)) and glutamatergic projection neurons (e.g., [L2/3 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4030059)). This broad but specific neuronal expression pattern suggests a role in regulating fundamental aspects of neuronal identity or function. Notably, [RFX3](/details-gene/5991) also demonstrates a significant role in the immune system, with a high CSI in [naive thymus-derived CD8-positive, alpha-beta T cell](/details-cell/CL0000900) (CSI: 38.82). This is consistent with studies implicating the RFX protein family in controlling lineage-specific gene expression during lymphocyte development [Link](https://doi.org/10.1128/mcb.19.6.3940). ## Pathways and Molecular Function [RFX3](/details-gene/5991) functions as a sequence-specific [DNA-binding transcription factor](/details-cell/GO:0003700) that operates primarily within the [nucleus](/details-cell/GO:0005634) as a component of [transcription regulator complexes](/details-cell/GO:0005667). Its activity is central to several key biological processes. The most prominent annotated function of [RFX3](/details-gene/5991) is its role in ciliogenesis, including [cilium assembly](/details-cell/GO:0060271) and [cilium-dependent cell motility](/details-cell/GO:0060285). This molecular function provides a direct mechanistic explanation for its high expression levels in the heavily ciliated [ependymal](/details-cell/CL0000065) and [choroid plexus epithelial cells](/details-cell/CL0000706) of the brain. As a transcription factor, [RFX3](/details-gene/5991) is capable of both activating and repressing gene expression, as indicated by its association with both [positive regulation of dna-templated transcription](/details-cell/GO:0045893) and [negative regulation of dna-templated transcription](/details-cell/GO:0045892). This dual functionality is supported by research showing that RFX family members can mediate both activation and repression depending on the cellular context and promoter architecture [Link](https://doi.org/10.1074/jbc.m209574200). Furthermore, functional annotations link [RFX3](/details-gene/5991) to pancreatic development, specifically [endocrine pancreas development](/details-cell/GO:0031018) and the [positive regulation of type b pancreatic cell development](/details-cell/GO:2000078). This is consistent with findings for other RFX family members, such as RFX6, which is essential for islet cell formation and insulin production [Link](https://doi.org/10.1038/nature08748), suggesting a conserved role for this transcription factor family in pancreatic biology. ## Research Directions Based on its distinct expression profile and functional annotations, several research avenues for [RFX3](/details-gene/5991) can be proposed, particularly concerning its roles in neuropathology and immunology. **Proposed Hypotheses:** 1. Given its high expression in ciliated CNS cells and its central role in [cilium assembly](/details-cell/GO:0060271), mutations or dysregulation of [RFX3](/details-gene/5991) may be a causative factor in congenital hydrocephalus or other neurological ciliopathies by disrupting cerebrospinal fluid dynamics mediated by [ependymal cell](/details-cell/CL0000065) cilia. 2. The high significance of [RFX3](/details-gene/5991) in [naive thymus-derived CD8-positive, alpha-beta T cells](/details-cell/CL0000900) suggests it acts as a gatekeeper of T cell quiescence. Its downregulation may be a prerequisite for T cell activation and differentiation, and its aberrant expression could contribute to T cell exhaustion or anergy in chronic diseases. **Suggested Experimental Approach:** To test the first hypothesis regarding the role of [RFX3](/details-gene/5991) in CNS ciliopathies, a conditional knockout mouse model could be developed. By crossing a floxed [RFX3](/details-gene/5991) allele mouse with a line expressing Cre recombinase under a ciliated cell-specific promoter (e.g., *Foxj1*-Cre), [RFX3](/details-gene/5991) could be selectively deleted in ependymal and choroid plexus cells. The resulting progeny could then be analyzed for phenotypes such as ventricular enlargement using non-invasive imaging (MRI), while tissue sections could be examined via immunofluorescence and electron microscopy to assess cilia structure, density, and orientation. **Therapeutic Potential:** As a transcription factor, [RFX3](/details-gene/5991) is a challenging molecule to target directly with small-molecule inhibitors or activators. However, its high specificity for certain cell types suggests that its downstream pathways could be more amenable to therapeutic intervention. In diseases driven by loss-of-function, such as certain genetic ciliopathies, gene therapy might be a viable long-term strategy. Conversely, if [RFX3](/details-gene/5991) overexpression contributes to pathology (e.g., by suppressing essential genes in immune cells), strategies aimed at inhibiting its activity through targeted degradation or by modulating its key downstream effectors could prove more fruitful than direct inhibition.

Genular Protein ID: 3214289378

Symbol: RFX3_HUMAN

Name: Transcription factor RFX3

UniProtKB Accession Codes:

Database IDs:

Citations:

PubMed ID: 8289803

Title: RFX1, a transactivator of hepatitis B virus enhancer I, belongs to a novel family of homodimeric and heterodimeric DNA-binding proteins.

PubMed ID: 8289803

DOI: 10.1128/mcb.14.2.1230-1244.1994

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15164053

Title: DNA sequence and analysis of human chromosome 9.

PubMed ID: 15164053

DOI: 10.1038/nature02465

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 10330134

Title: Dimeric RFX proteins contribute to the activity and lineage specificity of the interleukin-5 receptor alpha promoter through activation and repression domains.

PubMed ID: 10330134

DOI: 10.1128/mcb.19.6.3940

PubMed ID: 12411430

Title: Role for RFX transcription factors in non-neuronal cell-specific inactivation of the microtubule-associated protein MAP1A promoter.

PubMed ID: 12411430

DOI: 10.1074/jbc.m209574200

PubMed ID: 20148032

Title: Rfx6 directs islet formation and insulin production in mice and humans.

PubMed ID: 20148032

DOI: 10.1038/nature08748

Sequence Information:

  • Length: 749
  • Mass: 83530
  • Checksum: 531F5CFCA7A707C7
  • Sequence:
  • MQTSETGSDT GSTVTLQTSV ASQAAVPTQV VQQVPVQQQV QQVQTVQQVQ HVYPAQVQYV 
    EGSDTVYTNG AIRTTTYPYT ETQMYSQNTG GNYFDTQGSS AQVTTVVSSH SMVGTGGIQM 
    GVTGGQLISS SGGTYLIGNS MENSGHSVTH TTRASPATIE MAIETLQKSD GLSTHRSSLL 
    NSHLQWLLDN YETAEGVSLP RSTLYNHYLR HCQEHKLDPV NAASFGKLIR SIFMGLRTRR 
    LGTRGNSKYH YYGIRVKPDS PLNRLQEDMQ YMAMRQQPMQ QKQRYKPMQK VDGVADGFTG 
    SGQQTGTSVE QTVIAQSQHH QQFLDASRAL PEFGEVEISS LPDGTTFEDI KSLQSLYREH 
    CEAILDVVVN LQFSLIEKLW QTFWRYSPST PTDGTTITES SNLSEIESRL PKAKLITLCK 
    HESILKWMCN CDHGMYQALV EILIPDVLRP IPSALTQAIR NFAKSLEGWL SNAMNNIPQR 
    MIQTKVAAVS AFAQTLRRYT SLNHLAQAAR AVLQNTSQIN QMLSDLNRVD FANVQEQASW 
    VCQCDDNMVQ RLETDFKMTL QQQSTLEQWA AWLDNVMMQA LKPYEGRPSF PKAARQFLLK 
    WSFYSSMVIR DLTLRSAASF GSFHLIRLLY DEYMFYLVEH RVAQATGETP IAVMGEFGDL 
    NAVSPGNLDK DEGSEVESEM DEELDDSSEP QAKREKTELS QAFPVGCMQP VLETGVQPSL 
    LNPIHSEHIV TSTQTIRQCS ATGNTYTAV