Details for: UCHL3

Gene ID: 7347

Symbol: UCHL3

Ensembl ID: ENSG00000118939

Description: ubiquitin C-terminal hydrolase L3

Associated with

Cells (max top 100)

(Cell Significance Index and respective Thresholds are uniquely calculated using our advanced thresholding algorithms to reveal cell-specific gene markers)

  • Cell Name: polychromatophilic erythroblast (CL0000550)
    Fold Change: 154.2317
    Cell Significance Index: -23.9900
  • Cell Name: hematopoietic oligopotent progenitor cell (CL0002032)
    Fold Change: 86.4205
    Cell Significance Index: -21.9200
  • Cell Name: smooth muscle fiber of ileum (CL1000278)
    Fold Change: 67.1224
    Cell Significance Index: -31.6900
  • Cell Name: mucosal type mast cell (CL0000485)
    Fold Change: 65.5004
    Cell Significance Index: -26.6100
  • Cell Name: peripheral blood mononuclear cell (CL2000001)
    Fold Change: 59.9259
    Cell Significance Index: -30.8300
  • Cell Name: ileal goblet cell (CL1000326)
    Fold Change: 47.2451
    Cell Significance Index: -31.7000
  • Cell Name: ciliated cell of the bronchus (CL0002332)
    Fold Change: 28.1856
    Cell Significance Index: -26.9100
  • Cell Name: orthochromatic erythroblast (CL0000552)
    Fold Change: 23.4153
    Cell Significance Index: -28.8700
  • Cell Name: CD8-alpha-beta-positive, alpha-beta intraepithelial T cell (CL0000796)
    Fold Change: 10.3010
    Cell Significance Index: -27.6000
  • Cell Name: stromal cell of bone marrow (CL0010001)
    Fold Change: 7.6000
    Cell Significance Index: -29.9900
  • Cell Name: CD8-positive, alpha-beta regulatory T cell (CL0000795)
    Fold Change: 5.9809
    Cell Significance Index: -18.3700
  • Cell Name: epidermal Langerhans cell (CL0002457)
    Fold Change: 4.6788
    Cell Significance Index: -10.2400
  • Cell Name: thyroid follicular cell (CL0002258)
    Fold Change: 2.9789
    Cell Significance Index: 31.6500
  • Cell Name: placental villous trophoblast (CL2000060)
    Fold Change: 2.2438
    Cell Significance Index: 59.9100
  • Cell Name: bladder urothelial cell (CL1001428)
    Fold Change: 1.8030
    Cell Significance Index: 93.6600
  • Cell Name: umbrella cell of urothelium (CL4030056)
    Fold Change: 1.7223
    Cell Significance Index: 15.8600
  • Cell Name: epithelial cell of small intestine (CL0002254)
    Fold Change: 1.1339
    Cell Significance Index: 184.4200
  • Cell Name: lactocyte (CL0002325)
    Fold Change: 1.0986
    Cell Significance Index: 141.9300
  • Cell Name: progenitor cell of mammary luminal epithelium (CL0009116)
    Fold Change: 1.0979
    Cell Significance Index: 81.8300
  • Cell Name: luminal adaptive secretory precursor cell of mammary gland (CL4033057)
    Fold Change: 1.0811
    Cell Significance Index: 50.8100
  • Cell Name: oral mucosa squamous cell (CL1001576)
    Fold Change: 0.9706
    Cell Significance Index: 8.3400
  • Cell Name: intermediate cell of urothelium (CL4030055)
    Fold Change: 0.9213
    Cell Significance Index: 166.0900
  • Cell Name: basal cell of prostate epithelium (CL0002341)
    Fold Change: 0.8748
    Cell Significance Index: 23.8100
  • Cell Name: basal cell of urothelium (CL1000486)
    Fold Change: 0.8576
    Cell Significance Index: 105.4500
  • Cell Name: L2/3-6 intratelencephalic projecting glutamatergic neuron (CL4023040)
    Fold Change: 0.8275
    Cell Significance Index: 165.9900
  • Cell Name: luminal hormone-sensing cell of mammary gland (CL4033058)
    Fold Change: 0.8165
    Cell Significance Index: 5.0300
  • Cell Name: extravillous trophoblast (CL0008036)
    Fold Change: 0.7777
    Cell Significance Index: 4.8300
  • Cell Name: enteroendocrine cell of small intestine (CL0009006)
    Fold Change: 0.7549
    Cell Significance Index: 18.8700
  • Cell Name: acinar cell of salivary gland (CL0002623)
    Fold Change: 0.7539
    Cell Significance Index: 35.1500
  • Cell Name: sebum secreting cell (CL0000317)
    Fold Change: 0.7377
    Cell Significance Index: 52.1700
  • Cell Name: direct pathway medium spiny neuron (CL4023026)
    Fold Change: 0.7246
    Cell Significance Index: 27.4400
  • Cell Name: hair follicular keratinocyte (CL2000092)
    Fold Change: 0.7225
    Cell Significance Index: 319.4500
  • Cell Name: tuft cell of colon (CL0009041)
    Fold Change: 0.7177
    Cell Significance Index: 648.0200
  • Cell Name: indirect pathway medium spiny neuron (CL4023029)
    Fold Change: 0.6877
    Cell Significance Index: 30.4200
  • Cell Name: neoplastic cell (CL0001063)
    Fold Change: 0.6803
    Cell Significance Index: 135.0000
  • Cell Name: cell in vitro (CL0001034)
    Fold Change: 0.6726
    Cell Significance Index: 367.3100
  • Cell Name: conjunctival epithelial cell (CL1000432)
    Fold Change: 0.6142
    Cell Significance Index: 8.3800
  • Cell Name: microfold cell of epithelium of small intestine (CL1000353)
    Fold Change: 0.5963
    Cell Significance Index: 41.2400
  • Cell Name: cortical cell of adrenal gland (CL0002097)
    Fold Change: 0.5559
    Cell Significance Index: 14.9000
  • Cell Name: basal epithelial cell of tracheobronchial tree (CL0002329)
    Fold Change: 0.5489
    Cell Significance Index: 15.3400
  • Cell Name: Purkinje cell (CL0000121)
    Fold Change: 0.5414
    Cell Significance Index: 11.8600
  • Cell Name: paneth cell of epithelium of small intestine (CL1000343)
    Fold Change: 0.4380
    Cell Significance Index: 9.4900
  • Cell Name: enterocyte of epithelium of large intestine (CL0002071)
    Fold Change: 0.4343
    Cell Significance Index: 19.6900
  • Cell Name: tonsil germinal center B cell (CL2000006)
    Fold Change: 0.4260
    Cell Significance Index: 50.2400
  • Cell Name: obsolete caudal ganglionic eminence derived GABAergic cortical interneuron (CL4023070)
    Fold Change: 0.3912
    Cell Significance Index: 140.3300
  • Cell Name: early pro-B cell (CL0002046)
    Fold Change: 0.3896
    Cell Significance Index: 25.1300
  • Cell Name: forebrain neuroblast (CL1000042)
    Fold Change: 0.2756
    Cell Significance Index: 16.9400
  • Cell Name: GABAergic interneuron (CL0011005)
    Fold Change: 0.2481
    Cell Significance Index: 171.5900
  • Cell Name: enteroendocrine cell of colon (CL0009042)
    Fold Change: 0.2396
    Cell Significance Index: 45.6000
  • Cell Name: retinal progenitor cell (CL0002672)
    Fold Change: 0.2374
    Cell Significance Index: 13.3200
  • Cell Name: enterocyte of epithelium of small intestine (CL1000334)
    Fold Change: 0.2339
    Cell Significance Index: 6.7400
  • Cell Name: colon goblet cell (CL0009039)
    Fold Change: 0.2056
    Cell Significance Index: 20.3400
  • Cell Name: cardiac muscle myoblast (CL0000513)
    Fold Change: 0.1657
    Cell Significance Index: 12.7200
  • Cell Name: transit amplifying cell of colon (CL0009011)
    Fold Change: 0.1355
    Cell Significance Index: 4.3400
  • Cell Name: CD4-positive, alpha-beta memory T cell, CD45RO-positive (CL0001204)
    Fold Change: 0.1188
    Cell Significance Index: 3.4900
  • Cell Name: fibro/adipogenic progenitor cell (CL0009099)
    Fold Change: 0.1098
    Cell Significance Index: 5.5500
  • Cell Name: lung endothelial cell (CL1001567)
    Fold Change: 0.1095
    Cell Significance Index: 5.7100
  • Cell Name: stromal cell of ovary (CL0002132)
    Fold Change: 0.1070
    Cell Significance Index: 14.6900
  • Cell Name: pancreatic acinar cell (CL0002064)
    Fold Change: 0.0757
    Cell Significance Index: 12.9300
  • Cell Name: pulmonary alveolar epithelial cell (CL0000322)
    Fold Change: 0.0470
    Cell Significance Index: 35.5800
  • Cell Name: odontoblast (CL0000060)
    Fold Change: 0.0445
    Cell Significance Index: 5.7100
  • Cell Name: hippocampal granule cell (CL0001033)
    Fold Change: 0.0323
    Cell Significance Index: 2.1700
  • Cell Name: small intestine goblet cell (CL1000495)
    Fold Change: 0.0065
    Cell Significance Index: 0.2300
  • Cell Name: kidney loop of Henle cortical thick ascending limb epithelial cell (CL1001109)
    Fold Change: -0.0073
    Cell Significance Index: -5.3300
  • Cell Name: Hofbauer cell (CL3000001)
    Fold Change: -0.0123
    Cell Significance Index: -0.1000
  • Cell Name: pigmented epithelial cell (CL0000529)
    Fold Change: -0.0128
    Cell Significance Index: -24.1400
  • Cell Name: anterior lens cell (CL0002223)
    Fold Change: -0.0164
    Cell Significance Index: -30.2500
  • Cell Name: pancreatic A cell (CL0000171)
    Fold Change: -0.0190
    Cell Significance Index: -14.0800
  • Cell Name: lens epithelial cell (CL0002224)
    Fold Change: -0.0208
    Cell Significance Index: -31.9800
  • Cell Name: secondary lens fiber (CL0002225)
    Fold Change: -0.0233
    Cell Significance Index: -31.6900
  • Cell Name: abnormal cell (CL0001061)
    Fold Change: -0.0298
    Cell Significance Index: -3.0400
  • Cell Name: type B pancreatic cell (CL0000169)
    Fold Change: -0.0384
    Cell Significance Index: -21.6300
  • Cell Name: non-pigmented ciliary epithelial cell (CL0002304)
    Fold Change: -0.0428
    Cell Significance Index: -27.1700
  • Cell Name: pro-T cell (CL0000827)
    Fold Change: -0.0493
    Cell Significance Index: -1.2600
  • Cell Name: pancreatic PP cell (CL0002275)
    Fold Change: -0.0576
    Cell Significance Index: -35.9600
  • Cell Name: ciliary muscle cell (CL1000443)
    Fold Change: -0.0707
    Cell Significance Index: -32.1000
  • Cell Name: dopaminergic neuron (CL0000700)
    Fold Change: -0.0963
    Cell Significance Index: -27.7200
  • Cell Name: epithelial cell of stomach (CL0002178)
    Fold Change: -0.1501
    Cell Significance Index: -17.4900
  • Cell Name: eye photoreceptor cell (CL0000287)
    Fold Change: -0.1539
    Cell Significance Index: -9.7000
  • Cell Name: pancreatic D cell (CL0000173)
    Fold Change: -0.1619
    Cell Significance Index: -34.1100
  • Cell Name: pancreatic ductal cell (CL0002079)
    Fold Change: -0.1657
    Cell Significance Index: -18.9800
  • Cell Name: pigmented ciliary epithelial cell (CL0002303)
    Fold Change: -0.1963
    Cell Significance Index: -28.5400
  • Cell Name: mesenchymal cell (CL0008019)
    Fold Change: -0.2435
    Cell Significance Index: -4.0800
  • Cell Name: neutrophil progenitor cell (CL0000834)
    Fold Change: -0.2553
    Cell Significance Index: -6.8300
  • Cell Name: fibroblast of dermis (CL0002551)
    Fold Change: -0.2594
    Cell Significance Index: -5.4300
  • Cell Name: type I muscle cell (CL0002211)
    Fold Change: -0.2744
    Cell Significance Index: -6.7000
  • Cell Name: kidney loop of Henle descending limb epithelial cell (CL1001021)
    Fold Change: -0.2795
    Cell Significance Index: -22.1400
  • Cell Name: smooth muscle cell of sphincter of pupil (CL0002243)
    Fold Change: -0.2896
    Cell Significance Index: -30.1500
  • Cell Name: fibroblast of mammary gland (CL0002555)
    Fold Change: -0.2972
    Cell Significance Index: -8.5200
  • Cell Name: granulosa cell (CL0000501)
    Fold Change: -0.3012
    Cell Significance Index: -7.9200
  • Cell Name: endothelial cell of placenta (CL0009092)
    Fold Change: -0.3045
    Cell Significance Index: -1.8400
  • Cell Name: cortical interneuron (CL0008031)
    Fold Change: -0.3440
    Cell Significance Index: -8.2500
  • Cell Name: mesonephric nephron tubule epithelial cell (CL1000022)
    Fold Change: -0.3459
    Cell Significance Index: -12.0200
  • Cell Name: epithelial cell of urethra (CL1000296)
    Fold Change: -0.3490
    Cell Significance Index: -2.1600
  • Cell Name: centrilobular region hepatocyte (CL0019029)
    Fold Change: -0.3547
    Cell Significance Index: -5.9800
  • Cell Name: glycinergic neuron (CL1001509)
    Fold Change: -0.3783
    Cell Significance Index: -19.8600
  • Cell Name: intestinal tuft cell (CL0019032)
    Fold Change: -0.3804
    Cell Significance Index: -23.3200
  • Cell Name: peg cell (CL4033014)
    Fold Change: -0.4233
    Cell Significance Index: -9.7800
  • Cell Name: CD14-positive, CD16-negative classical monocyte (CL0002057)
    Fold Change: -0.4461
    Cell Significance Index: -8.2500
  • Cell Name: endothelial cell of venule (CL1000414)
    Fold Change: -0.4498
    Cell Significance Index: -5.1100

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Other Information

**Key Characteristics:** UCHL3 is a member of the ubiquitin C-terminal hydrolase (UCH) family, which consists of six isoforms (UCHL1-6). UCHL3 is a 24-kDa protein that is highly conserved across species, with a high degree of sequence similarity to other UCH family members. It is characterized by its ability to remove ubiquitin from proteins, a process known as deubiquitination, which is essential for protein degradation and regulation. **Pathways and Functions:** UCHL3 is involved in several key pathways, including: 1. **Protein Degradation:** UCHL3 plays a crucial role in the ubiquitin-proteasome system (UPS), which is responsible for the degradation of proteins that are no longer needed or damaged. By removing ubiquitin from proteins, UCHL3 regulates their stability and localization. 2. **Cell Cycle Regulation:** UCHL3 has been shown to regulate cell cycle progression by deubiquitinating proteins involved in the cell cycle, such as p53 and cyclin-dependent kinases. 3. **Apoptosis:** UCHL3 has been implicated in apoptosis, or programmed cell death, by deubiquitinating proteins involved in the apoptotic pathway, such as caspases. 4. **Metabolism:** UCHL3 is involved in the regulation of protein metabolism, including the degradation of proteins involved in various metabolic pathways. **Clinical Significance:** Dysregulation of UCHL3 has been implicated in several diseases, including: 1. **Cancer:** UCHL3 has been shown to be overexpressed in various types of cancer, including breast, lung, and colon cancer. Its overexpression has been associated with poor prognosis and increased tumor aggressiveness. 2. **Neurodegenerative Disorders:** UCHL3 has been implicated in neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease, where its dysregulation has been associated with protein aggregation and neuronal death. 3. **Autoimmune Conditions:** UCHL3 has been shown to play a role in autoimmune conditions, such as rheumatoid arthritis and lupus, where its dysregulation has been associated with protein degradation and immune system dysfunction. In conclusion, UCHL3 is a critical component of the ubiquitin-proteasome system that plays a vital role in protein degradation and regulation. Its dysregulation has been implicated in several diseases, highlighting the importance of further research into its role in human disease.

Genular Protein ID: 3826128715

Symbol: UCHL3_HUMAN

Name: Ubiquitin carboxyl-terminal hydrolase isozyme L3

UniProtKB Accession Codes:

P15374 B2R970 Q5TBK8 Q6IBE9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 BMRB 1 BRENDA 1 Bgee 1 BindingDB 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChEMBL 1 ChiTaRS 1 DIP 1 DMDM 1 DNASU 1 DisGeNET 1 DrugCentral 1 EC 1 EMBL 7 Ensembl 1 EvolutionaryTrace 1 ExpressionAtlas 1 GO 12 Gene3D 1 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 GuidetoPHARMACOLOGY 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 3 KEGG 1 MANE-Select 1 MEROPS 1 MIM 1 MINT 1 MassIVE 1 MetOSite 1 NCBI Taxonomy 1 OGP 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PDB 5 PDBsum 5 PIR 1 PRINTS 1 PRO 1 PROSITE 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 REPRODUCTION-2DPAGE 1 RNAct 1 Reactome 3 RefSeq 2 SABIO-RK 1 SIGNOR 1 SMR 1 STRING 1 SUPFAM 1 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 2530630

Title: The neuron-specific protein PGP 9.5 is a ubiquitin carboxyl-terminal hydrolase.

PubMed ID: 2530630

DOI: 10.1126/science.2530630

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 15057823

Title: The DNA sequence and analysis of human chromosome 13.

PubMed ID: 15057823

DOI: 10.1038/nature02379

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9790970

Title: Cleavage of the C-terminus of NEDD8 by UCH-L3.

PubMed ID: 9790970

DOI: 10.1006/bbrc.1998.9532

PubMed ID: 14595809

Title: Molecular profiling of the immune response in colon cancer using protein microarrays: occurrence of autoantibodies to ubiquitin C-terminal hydrolase L3.

PubMed ID: 14595809

DOI: 10.1002/pmic.200300594

PubMed ID: 15157086

Title: Substrate profiling of deubiquitin hydrolases with a positional scanning library and mass spectrometry.

PubMed ID: 15157086

DOI: 10.1021/bi049722j

PubMed ID: 17525332

Title: ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage.

PubMed ID: 17525332

DOI: 10.1126/science.1140321

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19047059

Title: Substrate filtering by the active site crossover loop in UCHL3 revealed by sortagging and gain-of-function mutations.

PubMed ID: 19047059

DOI: 10.1074/jbc.m807172200

PubMed ID: 19154770

Title: Ubiquitin dimers control the hydrolase activity of UCH-L3.

PubMed ID: 19154770

DOI: 10.1016/j.neuint.2008.12.013

PubMed ID: 20380862

Title: Skeletal muscles of Uchl3 knockout mice show polyubiquitinated protein accumulation and stress responses.

PubMed ID: 20380862

DOI: 10.1016/j.neuint.2010.03.021

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 21762696

Title: Mutant ubiquitin (UBB(+1)) associated with neurodegenerative disorders is hydrolyzed by ubiquitin C-terminal hydrolase L3 (UCH-L3).

PubMed ID: 21762696

DOI: 10.1016/j.febslet.2011.06.037

PubMed ID: 22689415

Title: Profiling ubiquitin linkage specificities of deubiquitinating enzymes with branched ubiquitin isopeptide probes.

PubMed ID: 22689415

DOI: 10.1002/cbic.201200261

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 24275569

Title: An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome.

PubMed ID: 24275569

DOI: 10.1016/j.jprot.2013.11.014

PubMed ID: 9233788

Title: Crystal structure of a deubiquitinating enzyme (human UCH-L3) at 1.8-A resolution.

PubMed ID: 9233788

DOI: 10.1093/emboj/16.13.3787

PubMed ID: 15531586

Title: Structure of the ubiquitin hydrolase UCH-L3 complexed with a suicide substrate.

PubMed ID: 15531586

DOI: 10.1074/jbc.m410770200

Sequence Information:

  • Length: 230
  • Mass: 26183
  • Checksum: 8ACACE6E1D86FD55
  • Sequence:
    • MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE 
KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK 
KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL 
YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA

Database document:

This is a preview of the gene's schema. Only a few entries are kept for 'singleCellExpressions,' 'mRNAExpressions,' and other large data arrays for visualization purposes. You can zoom in with the mouse wheel for a closer view, and the text will adjust automatically if necessary. For the full schema, download it here.