Details for: CKAP2

Gene ID: 26586

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: CKAP2

Ensembl ID: ENSG00000136108

Description: cytoskeleton associated protein 2

Cell Significance Landscape

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 11.62
    rCSI 13.42%
    PRS 66.84
  • large pre-B-II cell CL0000957
    CSI 9.47
    rCSI 27.04%
    PRS 82.11
  • neural crest cell CL0011012
    CSI 8.28
    rCSI 6.54%
    PRS 62.43
  • radial glial cell CL0000681
    CSI 8.06
    rCSI 11.19%
    PRS 73.26
  • erythrocyte CL0000232
    CSI 4.99
    rCSI 11.32%
    PRS 76.17
  • hematopoietic precursor cell CL0008001
    CSI 4.79
    rCSI 4.93%
    PRS 87.82
  • early lymphoid progenitor CL0000936
    CSI 4.44
    rCSI 3.9%
    PRS 80.06
  • hematopoietic stem cell CL0000037
    CSI 4.05
    rCSI 2.69%
    PRS 77.74
  • double negative thymocyte CL0002489
    CSI 4.02
    rCSI 2.8%
    PRS 86.24
  • effector CD4-positive, alpha-beta T cell CL0001044
    CSI 3.88
    rCSI 11.12%
    PRS 91.84
  • plasmacytoid dendritic cell, human CL0001058
    CSI 3.87
    rCSI 2.7%
    PRS 78.14
  • lung neuroendocrine cell CL1000223
    CSI 3.81
    rCSI 5.63%
    PRS 79.45
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 3.62
    rCSI 6.39%
    PRS 55.26
  • keratinocyte CL0000312
    CSI 3.33
    rCSI 2.79%
    PRS 77.96
  • plasmablast CL0000980
    CSI 3.3
    rCSI 2.6%
    PRS 80.8
  • pro-B cell CL0000826
    CSI 3.23
    rCSI 2.67%
    PRS 77.33
  • interneuron CL0000099
    CSI 3.15
    rCSI 6.33%
    PRS 64.22
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 3.06
    rCSI 3.12%
    PRS 85.55
  • mature T cell CL0002419
    CSI 2.95
    rCSI 2.29%
    PRS 89.91
  • precursor B cell CL0000817
    CSI 2.92
    rCSI 2.55%
    PRS 82.74
  • CD4-positive, CD25-positive, alpha-beta regulatory T cell CL0000792
    CSI 2.79
    rCSI 2.74%
    PRS 88.85
  • CD14-low, CD16-positive monocyte CL0002396
    CSI 2.78
    rCSI 2.14%
    PRS 77.09
  • erythroid lineage cell CL0000764
    CSI 2.74
    rCSI 17.63%
    PRS 86.08
  • fraction A pre-pro B cell CL0002045
    CSI 2.74
    rCSI 3.13%
    PRS 87.53
  • enteric smooth muscle cell CL0002504
    CSI 2.67
    rCSI 3.8%
    PRS 76.13
  • astrocyte CL0000127
    CSI 2.62
    rCSI 5.57%
    PRS 50.18
  • erythroid progenitor cell CL0000038
    CSI 2.49
    rCSI 14.28%
    PRS 81.33
  • CD4-positive helper T cell CL0000492
    CSI 2.42
    rCSI 1.83%
    PRS 87.37
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.41
    rCSI 7.1%
    PRS 76.2
  • common myeloid progenitor CL0000049
    CSI 2.41
    rCSI 1.94%
    PRS 77
  • granulocyte monocyte progenitor cell CL0000557
    CSI 2.4
    rCSI 2.08%
    PRS 79.26
  • OFF-bipolar cell CL0000750
    CSI 2.4
    rCSI 3.28%
    PRS 78.24
  • rod bipolar cell CL0000751
    CSI 2.37
    rCSI 4.26%
    PRS 67.76
  • club cell CL0000158
    CSI 2.32
    rCSI 3.4%
    PRS 69.44
  • mesodermal cell CL0000222
    CSI 2.22
    rCSI 2.66%
    PRS 72.82
  • common dendritic progenitor CL0001029
    CSI 2.21
    rCSI 2.78%
    PRS 84.22
  • pulmonary ionocyte CL0017000
    CSI 2.19
    rCSI 2.67%
    PRS 81.75
  • stem cell CL0000034
    CSI 2.18
    rCSI 2.11%
    PRS 67.13
  • ON-bipolar cell CL0000749
    CSI 2.18
    rCSI 3.24%
    PRS 75.15
  • retina horizontal cell CL0000745
    CSI 2.18
    rCSI 3.32%
    PRS 71.3
  • retinal bipolar neuron CL0000748
    CSI 2.16
    rCSI 4.04%
    PRS 62.49
  • transit amplifying cell of small intestine CL0009012
    CSI 2.12
    rCSI 9.31%
    PRS 84.71
  • class switched memory B cell CL0000972
    CSI 2.08
    rCSI 1.55%
    PRS 88.03
  • mature B cell CL0000785
    CSI 2.04
    rCSI 1.78%
    PRS 84.73
  • glioblast CL0000030
    CSI 2.02
    rCSI 3.22%
    PRS 66.28
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.98
    rCSI 1.79%
    PRS 72.52
  • primitive red blood cell CL0002355
    CSI 1.95
    rCSI 10.53%
    PRS 82.59
  • dendritic cell, human CL0001056
    CSI 1.89
    rCSI 2.91%
    PRS 83.89
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 1.87
    rCSI 2.4%
    PRS 70.98
  • mesenchymal cell CL0008019
    CSI 1.87
    rCSI 4.74%
    PRS 68.2
  • promyelocyte CL0000836
    CSI 1.86
    rCSI 2.68%
    PRS 81.82
  • fallopian tube secretory epithelial cell CL4030006
    CSI 1.77
    rCSI 1.71%
    PRS 74.11
  • peripheral nervous system neuron CL2000032
    CSI 1.49
    rCSI 2.03%
    PRS 66.03
  • neural cell CL0002319
    CSI 1.43
    rCSI 5.4%
    PRS 57.88
  • activated CD8-positive, alpha-beta T cell, human CL0001049
    CSI 1.4
    rCSI 2.4%
    PRS 89.1
  • placental villous trophoblast CL2000060
    CSI 1.4
    rCSI 2.17%
    PRS 73.75
  • promonocyte CL0000559
    CSI 1.37
    rCSI 2.35%
    PRS 82.05
  • Langerhans cell CL0000453
    CSI 1.33
    rCSI 2.04%
    PRS 87.43
  • thymocyte CL0000893
    CSI 1.32
    rCSI 4.67%
    PRS 94.53
  • erythroblast CL0000765
    CSI 1.18
    rCSI 3.14%
    PRS 81.9
  • myeloid dendritic cell, human CL0001057
    CSI 1.12
    rCSI 6.28%
    PRS 90.61
  • forebrain radial glial cell CL0013000
    CSI 1.11
    rCSI 3.57%
    PRS 77.65
  • respiratory basal cell CL0002633
    CSI 1.1
    rCSI 1.14%
    PRS 79.62
  • mature alpha-beta T cell CL0000791
    CSI 1.09
    rCSI 3.95%
    PRS 90.76
  • germinal center B cell CL0000844
    CSI 1.08
    rCSI 3.23%
    PRS 86.42
  • colon goblet cell CL0009039
    CSI 0.93
    rCSI 2.21%
    PRS 81.61
  • myeloid lineage restricted progenitor cell CL0000839
    CSI 0.87
    rCSI 4.5%
    PRS 91.07
  • neural progenitor cell CL0011020
    CSI 0.86
    rCSI 3.77%
    PRS 63.27
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 0.84
    rCSI 2.01%
    PRS 88.93
  • lymphoid lineage restricted progenitor cell CL0000838
    CSI 0.82
    rCSI 3.2%
    PRS 91.05
  • pluripotent stem cell CL0002248
    CSI 0.7
    rCSI 20.91%
    PRS 87.78

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [CKAP2](/details-gene/26586) (Cytoskeleton Associated Protein 2) is a protein-coding gene located on chromosome 13q14.3. It plays a crucial role in the regulation of the cell cycle, particularly during mitosis. As a key component of the microtubule cytoskeleton, [CKAP2](/details-gene/26586) is integral to the formation and function of the mitotic spindle, ensuring proper chromosome segregation. Its expression is highly significant in rapidly dividing cell populations, including various neural and hematopoietic progenitor cells, such as [neuroblast (sensu Nematoda and Protostomia)](/details-cell/CL0000338) and [large pre-B-II cell](/details-cell/CL0000957). Several studies have implicated the dysregulation and upregulation of [CKAP2](/details-gene/26586) in various human cancers, highlighting its importance in both normal cell proliferation and tumorigenesis ([Link](https://doi.org/10.1038/sj.onc.1202048), [Link](https://pubmed.ncbi.nlm.nih.gov/11234418/)). ## Cellular Roles and Expression Landscape The expression profile of [CKAP2](/details-gene/26586) underscores its central role in cell proliferation and development. **Overall**, the gene shows the highest significance in progenitor and precursor cell populations that are characterized by high rates of mitotic activity. Its most significant expression is observed in neural progenitors, including [neuroblast (sensu Nematoda and Protostomia)](/details-cell/CL0000338) (CSI: 11.62), [neural crest cell](/details-cell/CL0011012) (CSI: 8.28), and [radial glial cell](/details-cell/CL0000681) (CSI: 8.06), suggesting a fundamental role in neurogenesis. Concurrently, [CKAP2](/details-gene/26586) is a highly significant gene within the hematopoietic system, with prominent roles in [large pre-B-II cell](/details-cell/CL0000957) (CSI: 9.47), [hematopoietic precursor cell](/details-cell/CL0008001) (CSI: 4.79), [early lymphoid progenitor](/details-cell/CL0000936) (CSI: 4.44), and [hematopoietic stem cell](/details-cell/CL0000037) (CSI: 4.05). This pattern indicates that [CKAP2](/details-gene/26586) is a key molecular player supporting the rapid cell divisions required for the development and maintenance of both the nervous and immune systems. Its significance in more mature but still proliferative immune cells, like [effector CD4-positive, alpha-beta T cell](/details-cell/CL0001044) (CSI: 3.88) and [plasmablast](/details-cell/CL0000980) (CSI: 3.30), further supports its function in cell expansion. ## Pathways and Molecular Function Functional annotation aligns closely with the observed expression pattern, positioning [CKAP2](/details-gene/26586) as a core component of the cell division machinery. The gene is strongly associated with the [Mitotic spindle](/details-gene/GO:0072686), the [Centrosome](/details-gene/GO:0005813), and the [Microtubule cytoskeleton](/details-gene/GO:0015630). Its molecular function involves the [Negative regulation of microtubule depolymerization](/details-gene/GO:0007026), which is critical for stabilizing spindle microtubules during mitosis. Consistent with these annotations, [CKAP2](/details-gene/26586) is a key participant in the biological process of [Mitotic cytokinesis](/details-gene/GO:0000281). Research has shown that it is a spindle-associated protein that is specifically degraded by the anaphase-promoting complex/cyclosome (APC/C) during mitotic exit, a process essential for the completion of cell division ([Link](https://doi.org/10.1074/jbc.m701688200)). Additionally, its annotation for the [Apoptotic process](/details-gene/GO:0006915) suggests a potential link between the mitotic machinery and programmed cell death pathways, possibly as part of a checkpoint mechanism. ## Research Directions The established role of [CKAP2](/details-gene/26586) in mitosis and its documented upregulation in malignancies such as diffuse large B-cell lymphomas, gastric adenocarcinomas, and cutaneous T-cell lymphomas make it a compelling subject for further investigation ([Link](https://doi.org/10.1038/sj.onc.1202048), [Link](https://doi.org/10.1007/s00432-003-0484-0), [Link](https://doi.org/10.1073/pnas.98.2.629)). Its high expression in proliferative precursor cells suggests that its dysregulation could be an early event in tumorigenesis. Based on the available data, several testable hypotheses can be proposed: 1. Overexpression of [CKAP2](/details-gene/26586) in hematopoietic progenitor cells, such as [large pre-B-II cell](/details-cell/CL0000957), directly contributes to malignant transformation by promoting mitotic errors and aneuploidy due to hyper-stabilized mitotic spindles. 2. Beyond its structural role, [CKAP2](/details-gene/26586) may function as a node integrating cell cycle progression and survival signals, with its upregulation in cancer cells contributing to the evasion of apoptosis ([GO:0006915]) that would normally be triggered by mitotic defects. To test the first hypothesis, a specific experimental approach could be employed: * **Experiment:** To determine if [CKAP2](/details-gene/26586) drives genomic instability, one could use a lentiviral system to induce stable overexpression of [CKAP2](/details-gene/26586) in primary hematopoietic stem and progenitor cells. These modified cells could then be cultured and assessed for mitotic defects using high-resolution live-cell imaging to monitor spindle dynamics and chromosome segregation. Subsequent analysis via spectral karyotyping (SKY) or single-cell DNA sequencing would provide a quantitative measure of aneuploidy and other chromosomal aberrations. **Therapeutic Potential:** Given its critical role in cell division and its frequent overexpression in various cancers, [CKAP2](/details-gene/26586) represents a promising therapeutic target. As an intracellular protein essential for proliferation, it is a candidate for targeting with small molecule inhibitors. A therapeutic strategy based on **inhibition** could selectively halt the proliferation of tumor cells that are highly dependent on [CKAP2](/details-gene/26586) for division. However, its high significance in normal hematopoietic and neural progenitor populations raises potential concerns for on-target toxicity, which would necessitate a carefully managed therapeutic window or targeted delivery systems.

Genular Protein ID: 4031155912

Symbol: CKAP2_HUMAN

Name: Cytoskeleton-associated protein 2

UniProtKB Accession Codes:

Q8WWK9 A2BDE0 A5YM58 B4DR35 E9PD90 Q3KRA5 Q5VXB4 Q8IWV5 Q8IWV6 Q96FH9 Q9H012 Q9H0D0 Q9H988 Q9HC49 Q9NVG4

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 4 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 ELM 1 EMBL 14 Ensembl 4 ExpressionAtlas 1 GO 10 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 GlyGen 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 2 KEGG 1 MANE-Select 1 MIM 1 MINT 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 1 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 2 Pumba 1 RNAct 1 RefSeq 4 SIGNOR 1 STRING 1 SignaLink 1 SwissPalm 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9771967

Title: Identification of a novel cDNA, encoding a cytoskeletal associated protein, differentially expressed in diffuse large B-cell lymphomas.

PubMed ID: 9771967

DOI: 10.1038/sj.onc.1202048

PubMed ID: 11234418

Title: Evolutionarily-conserved gene CKAP2, located in region 13q14.3 of the human genome, is frequently rearranged in various tumors.

PubMed ID: 11234418

PubMed ID: 12942315

Title: Up-regulation of cytoskeletal-associated protein 2 in primary human gastric adenocarcinomas.

PubMed ID: 12942315

DOI: 10.1007/s00432-003-0484-0

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 15057823

Title: The DNA sequence and analysis of human chromosome 13.

PubMed ID: 15057823

DOI: 10.1038/nature02379

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 11149944

Title: Serological detection of cutaneous T-cell lymphoma-associated antigens.

PubMed ID: 11149944

DOI: 10.1073/pnas.98.2.629

PubMed ID: 11230166

Title: Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs.

PubMed ID: 11230166

DOI: 10.1101/gr.gr1547r

PubMed ID: 17376772

Title: CKAP2 is a spindle-associated protein degraded by APC/C-Cdh1 during mitotic exit.

PubMed ID: 17376772

DOI: 10.1074/jbc.m701688200

PubMed ID: 18669648

Title: A quantitative atlas of mitotic phosphorylation.

PubMed ID: 18669648

DOI: 10.1073/pnas.0805139105

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 20068231

Title: Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis.

PubMed ID: 20068231

DOI: 10.1126/scisignal.2000475

PubMed ID: 21269460

Title: Initial characterization of the human central proteome.

PubMed ID: 21269460

DOI: 10.1186/1752-0509-5-17

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

Sequence Information:

  • Length: 683
  • Mass: 76987
  • Checksum: 15287A7D860A4B23
  • Sequence:
    • MSTPAVPQDL QLPPSQRAQS AFKEQRRQKL KEHLLRRKTL FAYKQENEML SSSRDQRVVT 
SEDQVQEGTK VLKLKTKMAD KENMKRPAES KNNTVVGKHC IPLKPSNELT NSTVVIDTHK 
PKDSNQTPHL LLTEDDPQSQ HMTLSQAFHL KNNSKKKQMT TEKQKQDANM PKKPVLGSYR 
GQIVQSKINS FRKPLQVKDE SSAATKKLSA TIPKATKPQP VNTSSVTVKS NRSSNMTATT 
KFVSTTSQNT QLVRPPIRSH HSNTRDTVKQ GISRTSANVT IRKGPHEKEL LQSKTALSSV 
KTSSSQGIIR NKTLSRSIAS EVIARPASLS NDKLMEKSEP VDQRRHTAGK AIVDSRSAQP 
KETSEERKAR LSEWKAGKGR VLKRPPNSVV TQHEPAGQNE KPVGSFWTTM AEEDEQRLFT 
EKVNNTFSEC LNLINEGCPK EDILVTLNDL IKNIPDAKKL VKYWICLALI EPITSPIENI 
IAIYEKAILA GAQPIEEMRH TIVDILTMKS QEKANLGENM EKSCASKEEV KEVSIEDTGV 
DVDPEKLEME SKLHRNLLFQ DCEKEQDNKT KDPTHDVKTP NTETRTSCLI KYNVSTTPYL 
QSVKKKVQFD GTNSAFKELK FLTPVRRSRR LQEKTSKLPD MLKDHYPCVS SLEQLTELGR 
ETDAFVCRPN AALCRVYYEA DTT