Details for: ZNF184

Gene ID: 7738

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF184

Ensembl ID: ENSG00000096654

Description: zinc finger protein 184

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • progenitor cell CL0011026
    CSI 6.34
    rCSI 13.48%
    PRS 96.47
  • basal cell of epidermis CL0002187
    CSI 1.48
    rCSI 2.63%
    PRS 84.21
  • L2/3-6 intratelencephalic projecting glutamatergic neuron CL4023040
    CSI 1.34
    rCSI 3.26%
    PRS 94.41
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 0.87
    rCSI 3.13%
    PRS 94.81

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

Loading network (please wait)...

Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF184](/details-gene/7738) is a protein-coding gene located on human chromosome 6p22.1, which encodes Zinc Finger Protein 184, a member of the Krueppel-related family of zinc finger proteins [[Link](https://doi.org/10.1006/geno.1996.4583)]. Functional annotations strongly indicate its role as a nuclear DNA-binding transcription factor involved in the regulation of gene expression via RNA polymerase II. **Overall** expression data highlight its profound significance in [progenitor cell](/details-cell/CL0011026) populations, with a CSI of 6.34, suggesting a fundamental role in regulating cellular stemness or differentiation. It also shows notable expression in the [basal cell of epidermis](/details-cell/CL0002187) and specific subtypes of cortical neurons, pointing to specialized roles in tissue maintenance and neuronal identity. The gene is associated with the OMIM entry [602277](https://omim.org/entry/602277). ## Cellular Roles and Expression Landscape The expression profile of [ZNF184](/details-gene/7738) suggests a highly specific function in undifferentiated or actively regenerating cell populations and the central nervous system. Its most significant expression is observed in [progenitor cell](/details-cell/CL0011026)s, where it stands out as a top marker. This is complemented by its elevated expression in the [basal cell of epidermis](/details-cell/CL0002187), a layer rich in skin stem and progenitor cells responsible for epidermal turnover. This pattern is consistent with a potential role for [ZNF184](/details-gene/7738) in maintaining the undifferentiated state or directing early lineage commitment in these tissues. In addition to its role in progenitor populations, [ZNF184](/details-gene/7738) is also significantly expressed in specific neuronal subtypes, including [L2/3-6 intratelencephalic projecting glutamatergic neuron](/details-cell/CL4023040) and [L5 extratelencephalic projecting glutamatergic cortical neuron](/details-cell/CL4023041). This suggests that beyond a general progenitor function, [ZNF184](/details-gene/7738) may also be involved in establishing or maintaining the transcriptional programs that define the mature identity and function of these specialized cortical neurons. ## Pathways and Molecular Function Consistent with its predicted protein structure, the molecular function of [ZNF184](/details-gene/7738) is centered on transcriptional regulation. Gene Ontology annotations confirm its localization to the [nucleus](/details-cell/GO:0005634) and its activity as a sequence-specific DNA-binding transcription factor ([GO:0000978](https://www.ebi.ac.uk/QuickGO/term/GO:0000978), [GO:0000981](https://www.ebi.ac.uk/QuickGO/term/GO:0000981)). It is specifically implicated in the [regulation of transcription by RNA polymerase II](/details-cell/GO:0006357), a fundamental process for protein-coding gene expression. Its function also includes [zinc ion binding](/details-cell/GO:0008270), which is essential for the structural integrity and DNA-binding capacity of its zinc finger domains. Reactome pathway analysis further places [ZNF184](/details-gene/7738) within the core machinery of [gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) and, more specifically, [RNA polymerase II transcription](https://reactome.org/content/detail/R-HSA-73857). This functional role is directly aligned with its high significance in [progenitor cell](/details-cell/CL0011026)s, where precise transcriptional control is paramount for orchestrating complex developmental and cell fate decisions. ## Research Directions The available data point to [ZNF184](/details-gene/7738) as a crucial transcriptional regulator in progenitor and specific neuronal cell types. This provides a basis for several testable hypotheses regarding its function in development and disease. **Proposed Hypotheses:** 1. [ZNF184](/details-gene/7738) acts as a gatekeeper of the progenitor state in epidermal and other stem cell populations. Its downregulation may be a prerequisite for terminal differentiation, while its aberrant expression could contribute to hyperproliferative disorders or cancer. 2. In the cerebral cortex, [ZNF184](/details-gene/7738) regulates a specific suite of genes required for the functional identity and circuit integration of intratelencephalic and extratelencephalic projecting glutamatergic neurons. **Experimental Approach:** To test the first hypothesis regarding its role in progenitor cells, a compelling experiment would be to use a CRISPR-Cas9 knockout or CRISPRi-mediated knockdown of [ZNF184](/details-gene/7738) in primary human keratinocytes or induced pluripotent stem cells (iPSCs). Following genetic perturbation, these cells could be subjected to differentiation protocols. Single-cell RNA sequencing (scRNA-seq) could then be used to assess whether the loss of [ZNF184](/details-gene/7738) function prematurely pushes cells towards a differentiated state or alters their differentiation trajectory, providing direct evidence of its role in maintaining stemness. **Therapeutic Potential:** As a nuclear transcription factor, [ZNF184](/details-gene/7738) presents a challenging direct target for small-molecule inhibitors. However, its high specificity for progenitor and basal cell populations suggests it could be relevant in diseases characterized by the dysregulation of these cells, such as basal cell carcinoma or other epithelial cancers. If [ZNF184](/details-gene/7738) is found to maintain a proliferative, undifferentiated state, targeting its unique downstream effectors or developing strategies for its transcriptional repression could represent a viable therapeutic avenue. The therapeutic strategy would likely focus on **inhibition** of its function or expression to promote differentiation and arrest tumor growth.

Genular Protein ID: 913691021

Symbol: ZN184_HUMAN

Name: Zinc finger protein 184

UniProtKB Accession Codes:

Q99676 B2R715 O60792 Q8TBA9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 1 CDD 1 CTD 1 ChiTaRS 1 DMDM 1 DNASU 1 DisGeNET 1 EMBL 4 Ensembl 3 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GeneWiki 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 4 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 1 Pumba 1 RNAct 1 Reactome 1 RefSeq 5 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 14574404

Title: The DNA sequence and analysis of human chromosome 6.

PubMed ID: 14574404

DOI: 10.1038/nature02055

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 9073517

Title: Identification of a novel Krueppel-related zinc finger gene (ZNF184) mapping to 6p21.3.

PubMed ID: 9073517

DOI: 10.1006/geno.1996.4583

PubMed ID: 19690332

Title: Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions.

PubMed ID: 19690332

DOI: 10.1126/scisignal.2000007

PubMed ID: 23186163

Title: Toward a comprehensive characterization of a human cancer cell phosphoproteome.

PubMed ID: 23186163

DOI: 10.1021/pr300630k

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

Sequence Information:

  • Length: 751
  • Mass: 86174
  • Checksum: 249DFB302C6BC97D
  • Sequence:
    • MEDLSSPDST LLQGGHNLLS SASFQEAVTF KDVIVDFTQE EWKQLDPGQR DLFRDVTLEN 
YTHLVSIGLQ VSKPDVISQL EQGTEPWIME PSIPVGTCAD WETRLENSVS APEPDISEEE 
LSPEVIVEKH KRDDSWSSNL LESWEYEGSL ERQQANQQTL PKEIKVTEKT IPSWEKGPVN 
NEFGKSVNVS SNLVTQEPSP EETSTKRSIK QNSNPVKKEK SCKCNECGKA FSYCSALIRH 
QRTHTGEKPY KCNECEKAFS RSENLINHQR IHTGDKPYKC DQCGKGFIEG PSLTQHQRIH 
TGEKPYKCDE CGKAFSQRTH LVQHQRIHTG EKPYTCNECG KAFSQRGHFM EHQKIHTGEK 
PFKCDECDKT FTRSTHLTQH QKIHTGEKTY KCNECGKAFN GPSTFIRHHM IHTGEKPYEC 
NECGKAFSQH SNLTQHQKTH TGEKPYDCAE CGKSFSYWSS LAQHLKIHTG EKPYKCNECG 
KAFSYCSSLT QHRRIHTREK PFECSECGKA FSYLSNLNQH QKTHTQEKAY ECKECGKAFI 
RSSSLAKHER IHTGEKPYQC HECGKTFSYG SSLIQHRKIH TGERPYKCNE CGRAFNQNIH 
LTQHKRIHTG AKPYECAECG KAFRHCSSLA QHQKTHTEEK PYQCNKCEKT FSQSSHLTQH 
QRIHTGEKPY KCNECDKAFS RSTHLTEHQN THTGEKPYNC NECRKTFSQS TYLIQHQRIH 
SGEKPFGCND CGKSFRYRSA LNKHQRLHPG I