Details for: ZNF195

Gene ID: 7748

Gene Type:  Protein-coding  - A gene that serves as a template for producing a messenger RNA (mRNA) molecule, which is then translated into a functional protein.

Symbol: ZNF195

Ensembl ID: ENSG00000005801

Description: zinc finger protein 195

Selected Context(s):  Overall

Cell Significance Landscape

Contexts:

Associated with

  • Gene expression (transcription)
    (R-HSA-74160)
  • Generic transcription pathway
    (R-HSA-212436)
  • Rna polymerase ii transcription
    (R-HSA-73857)
  • Dna-binding transcription factor activity, rna polymerase ii-specific
    (GO:0000981)
  • Nucleus
    (GO:0005634)
  • Regulation of dna-templated transcription
    (GO:0006355)
  • Regulation of transcription by rna polymerase ii
    (GO:0006357)
  • Rna polymerase ii cis-regulatory region sequence-specific dna binding
    (GO:0000978)
  • Zinc ion binding
    (GO:0008270)

Significant Cells

Cell Significance Index (CSI) scores for the chosen context(s)

  • plasmablast CL0000980
    CSI 9.12
    rCSI 7.17%
    PRS 91.27
  • caudal ganglionic eminence derived cortical interneuron CL4023064
    CSI 5.97
    rCSI 10.55%
    PRS 73.65
  • mature B cell CL0000785
    CSI 4.36
    rCSI 3.79%
    PRS 94.91
  • pvalb GABAergic cortical interneuron CL4023018
    CSI 3.5
    rCSI 4.36%
    PRS 71.94
  • hematopoietic multipotent progenitor cell CL0000837
    CSI 3.38
    rCSI 8.13%
    PRS 96.07
  • double negative thymocyte CL0002489
    CSI 3.29
    rCSI 2.29%
    PRS 96.29
  • interneuron CL0000099
    CSI 2.88
    rCSI 5.78%
    PRS 81.66
  • enteroendocrine cell CL0000164
    CSI 2.79
    rCSI 3.81%
    PRS 87.3
  • pro-B cell CL0000826
    CSI 2.77
    rCSI 2.3%
    PRS 90.35
  • ionocyte CL0005006
    CSI 2.73
    rCSI 2.92%
    PRS 90.14
  • hematopoietic stem cell CL0000037
    CSI 2.64
    rCSI 1.76%
    PRS 90.31
  • common myeloid progenitor CL0000049
    CSI 2.61
    rCSI 2.11%
    PRS 90.2
  • intestinal epithelial cell CL0002563
    CSI 2.55
    rCSI 2.66%
    PRS 86.16
  • double-positive, alpha-beta thymocyte CL0000809
    CSI 2.51
    rCSI 2.55%
    PRS 94.17
  • plasmacytoid dendritic cell, human CL0001058
    CSI 2.48
    rCSI 1.73%
    PRS 91.6
  • corticothalamic-projecting glutamatergic cortical neuron CL4023013
    CSI 2.44
    rCSI 14.35%
    PRS 74.59
  • neural crest cell CL0011012
    CSI 2.42
    rCSI 1.91%
    PRS 80.49
  • hematopoietic precursor cell CL0008001
    CSI 2.41
    rCSI 2.48%
    PRS 94.95
  • cerebral cortex GABAergic interneuron CL0010011
    CSI 2.41
    rCSI 7.1%
    PRS 88.9
  • ciliated epithelial cell CL0000067
    CSI 2.39
    rCSI 2.1%
    PRS 79.46
  • early lymphoid progenitor CL0000936
    CSI 2.36
    rCSI 2.07%
    PRS 92.12
  • cerebral cortex endothelial cell CL1001602
    CSI 2.35
    rCSI 4.06%
    PRS 82.42
  • class switched memory B cell CL0000972
    CSI 2.34
    rCSI 1.75%
    PRS 95.79
  • rod bipolar cell CL0000751
    CSI 2.33
    rCSI 4.18%
    PRS 83.01
  • transit amplifying cell of colon CL0009011
    CSI 2.33
    rCSI 2.73%
    PRS 88.97
  • retinal bipolar neuron CL0000748
    CSI 2.28
    rCSI 4.28%
    PRS 79.16
  • stem cell CL0000034
    CSI 2.23
    rCSI 2.15%
    PRS 83.95
  • respiratory suprabasal cell CL4033048
    CSI 2.03
    rCSI 2.6%
    PRS 90.43
  • retina horizontal cell CL0000745
    CSI 2
    rCSI 3.05%
    PRS 85.37
  • choroid plexus epithelial cell CL0000706
    CSI 1.99
    rCSI 3.25%
    PRS 80.53
  • megakaryocyte-erythroid progenitor cell CL0000050
    CSI 1.94
    rCSI 1.75%
    PRS 87.34
  • neuroblast (sensu Nematoda and Protostomia) CL0000338
    CSI 1.9
    rCSI 2.2%
    PRS 81.19
  • granulocyte monocyte progenitor cell CL0000557
    CSI 1.89
    rCSI 1.63%
    PRS 90.95
  • neuroblast (sensu Vertebrata) CL0000031
    CSI 1.81
    rCSI 2.33%
    PRS 84.97
  • radial glial cell CL0000681
    CSI 1.71
    rCSI 2.37%
    PRS 86.88
  • lamp5 GABAergic cortical interneuron CL4023011
    CSI 1.69
    rCSI 2.83%
    PRS 74.14
  • peripheral nervous system neuron CL2000032
    CSI 1.68
    rCSI 2.28%
    PRS 81.73
  • astrocyte of the cerebral cortex CL0002605
    CSI 1.66
    rCSI 3.71%
    PRS 74.78
  • common dendritic progenitor CL0001029
    CSI 1.62
    rCSI 2.04%
    PRS 94.02
  • respiratory basal cell CL0002633
    CSI 1.52
    rCSI 1.57%
    PRS 90.38
  • progenitor cell CL0011026
    CSI 1.49
    rCSI 3.16%
    PRS 82.63
  • retinal ganglion cell CL0000740
    CSI 1.45
    rCSI 3.21%
    PRS 76.92
  • kidney connecting tubule epithelial cell CL1000768
    CSI 1.45
    rCSI 3.68%
    PRS 81.42
  • L5 extratelencephalic projecting glutamatergic cortical neuron CL4023041
    CSI 1.17
    rCSI 4.19%
    PRS 72.11
  • L6b glutamatergic cortical neuron CL4023038
    CSI 1.08
    rCSI 3.36%
    PRS 75.54
  • chandelier pvalb GABAergic cortical interneuron CL4023036
    CSI 0.94
    rCSI 2.93%
    PRS 77.5
  • glial cell CL0000125
    CSI 0.84
    rCSI 3.21%
    PRS 81.34
  • near-projecting glutamatergic cortical neuron CL4023012
    CSI 0.75
    rCSI 2.83%
    PRS 74.45
  • podocyte CL0000653
    CSI 0.62
    rCSI 2.75%
    PRS 89.04
  • central nervous system neuron CL2000029
    CSI 0.57
    rCSI 4.17%
    PRS 78.93
  • direct pathway medium spiny neuron CL4023026
    CSI 0.54
    rCSI 12.9%
    PRS 71.8
  • blood vessel smooth muscle cell CL0019018
    CSI 0.44
    rCSI 3.61%
    PRS 84.81
  • indirect pathway medium spiny neuron CL4023029
    CSI 0.36
    rCSI 8.66%
    PRS 72.21

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this specific cell.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.

Cell ID: Standard Cell Ontology term used for mapping and comparing cells across experiments. Ensures consistency in analyzing cellular functions across tissues.
Fold Change: Represents the ratio of the current Cell Significance Index to the Cell Significance Index Threshold, indicating how much the gene expression has changed compared to a baseline.
Cell Significance Index: Reflects how strongly a gene is expressed in this cell type. Calculated using techniques like effect size estimation and bootstrapping for reliability.
Network Configuration

Explore relationships of the current gene. Select an Interaction Source: 'ONTOLOGY' for shared pathways (GO/Reactome) or 'STRING' for protein-protein interactions. Further refine by selecting context genes and comparing Cell Significance Index (CSI) scores between baseline and target cell types and their specific contexts.

Comma-separated if multiple.
Comma-separated if multiple.
Legend:
  • Query Gene
  • Node Color (Target Cell CSI, relative to current network):
    • Very High
    • High
    • Medium
    • Low
    • Very Low
    • CSI N/A
  • Node Size: Proportional to Target Cell CSI magnitude
  • STRING PPI Edge
  • Shared Pathway Edge (ONTOLOGY)

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Other Information

This section provides additional information about the gene, including a description generated by an AI language model and details about associated proteins.

## Summary [ZNF195](/details-gene/7748) is a protein-coding gene located on chromosome 11p15.4 that encodes Zinc Finger Protein 195, a member of the KRAB zinc finger gene family [[Link](https://doi.org/10.1006/geno.1997.4958)]. Functional annotations consistently point to its role as a DNA-binding transcription factor that regulates gene expression by interacting with RNA polymerase II [[GO:0000981](https://www.ebi.ac.uk/QuickGO/term/GO:0000981), [R-HSA-74160](https://reactome.org/content/detail/R-HSA-74160)]. **Overall**, expression data reveals a highly specific and significant role for [ZNF195](/details-gene/7748) in distinct cell lineages. It is most prominently expressed in cells of the B-lymphocyte lineage, particularly terminally differentiated [plasmablast](/details-cell/CL0000980)s, and also shows high significance in specific subtypes of cortical [interneuron](/details-cell/CL0000099)s. This bimodal expression pattern suggests a critical function in regulating developmental and terminal differentiation programs in both the hematopoietic and nervous systems. ## Cellular Roles and Expression Landscape The expression profile of [ZNF195](/details-gene/7748) highlights its specialized function in two primary biological systems: the immune system and the central nervous system. Within the immune system, [ZNF195](/details-gene/7748) demonstrates a strong association with the B-cell developmental axis. Its highest significance score is observed in [plasmablast](/details-cell/CL0000980)s (CSI: 9.12), the immediate precursors to antibody-secreting plasma cells. High significance is also noted in earlier stages, including [mature B cell](/details-cell/CL0000785)s (CSI: 4.36) and [pro-B cell](/details-cell/CL0000826)s (CSI: 2.77). This pattern suggests a continuous and potentially escalating role for [ZNF195](/details-gene/7748) throughout B-cell maturation and terminal differentiation. Its expression in hematopoietic progenitors, including [hematopoietic multipotent progenitor cell](/details-cell/CL0000837)s and [hematopoietic stem cell](/details-cell/CL0000037)s, further indicates a function in early lineage commitment. Concurrently, [ZNF195](/details-gene/7748) exhibits high significance in specific neuronal populations. It is a key marker for [caudal ganglionic eminence derived cortical interneuron](/details-cell/CL4023064)s (CSI: 5.97) and [pvalb GABAergic cortical interneuron](/details-cell/CL4023018)s (CSI: 3.50). This distinct neuronal expression profile implies a specialized role in the development, specification, or function of these inhibitory interneuron subtypes within the cortex. ## Pathways and Molecular Function The molecular function of [ZNF195](/details-gene/7748) is firmly established as a nuclear transcription factor. Gene Ontology annotations describe it as a sequence-specific DNA-binding protein [[GO:0000978](https://www.ebi.ac.uk/QuickGO/term/GO:0000978)] that regulates DNA-templated transcription [[GO:0006355](https://www.ebi.ac.uk/QuickGO/term/GO:0006355)]. Its activity is specifically linked to RNA polymerase II, which is responsible for transcribing all protein-coding genes [[GO:0006357](https://www.ebi.ac.uk/QuickGO/term/GO:0006357)]. This is corroborated by its involvement in the [Gene expression (transcription)](https://reactome.org/content/detail/R-HSA-74160) pathway. Consistent with its role as a transcription factor, [ZNF195](/details-gene/7748) is localized to the [nucleus](/details-cell/GO:0005634). Its high expression in developing hematopoietic cells and terminally differentiating [plasmablast](/details-cell/CL0000980)s suggests it may orchestrate complex gene expression programs required for cell fate decisions and the acquisition of specialized functions, such as high-rate antibody production. Similarly, its role in cortical [interneuron](/details-cell/CL0000099)s is likely to involve the transcriptional regulation of genes that define their unique electrophysiological properties and synaptic connectivity. ## Research Directions The dual-lineage specificity of [ZNF195](/details-gene/7748) in both the immune and nervous systems presents several avenues for future research. Understanding the precise transcriptional networks it controls in these distinct contexts is a key objective. **Proposed Hypotheses:** 1. Given its peak expression in [plasmablast](/details-cell/CL0000980)s, [ZNF195](/details-gene/7748) functions as a master regulator of the transcriptional program governing terminal B-cell differentiation and the establishment of the secretory apparatus required for antibody production. 2. The high significance of [ZNF195](/details-gene/7748) in specific GABAergic [interneuron](/details-cell/CL0000099) subtypes suggests it is essential for the specification or functional maturation of these neurons, potentially by regulating genes involved in neurotransmitter synthesis, receptor expression, or ion channel function. **Experimental Approach:** To test the first hypothesis regarding its role in B-cell differentiation, a conditional knockout mouse model could be generated (e.g., using a *Cd19-Cre* driver to delete *Zfp195* specifically in the B-cell lineage). Following immunization, these mice could be analyzed for defects in germinal center formation, [plasmablast](/details-cell/CL0000980) development, and serum antibody levels using flow cytometry and ELISA. Complementary mechanistic studies, such as ChIP-seq for ZFP195 in a human plasmablast-like cell line, could identify its direct downstream gene targets, revealing the specific pathways it regulates (e.g., Unfolded Protein Response, ER/Golgi biogenesis). **Therapeutic Potential:** As a nuclear DNA-binding protein, [ZNF195](/details-gene/7748) is a challenging direct target for conventional small molecule inhibitors or antibody-based therapies. However, its high and specific expression in [plasmablast](/details-cell/CL0000980)s could make it a potential target in B-cell-derived malignancies like multiple myeloma. If proven to be an essential dependency in such cancers, strategies aimed at its targeted protein degradation (e.g., PROTACs) or the inhibition of its critical downstream effectors could represent viable therapeutic avenues. Inhibition, rather than activation, would likely be the goal to disrupt the function of the malignant cells.

Genular Protein ID: 32299700

Symbol: ZN195_HUMAN

Name: Zinc finger protein 195

UniProtKB Accession Codes:

O14628 A8K234 B3KTK2 B4DEL0 C9JLY9 L7MNK2 Q0VAJ6 Q658N8 Q6ZNA9

Database IDs:

AGR 1 AlphaFoldDB 1 Antibodypedia 1 Bgee 1 BioGRID 1 BioGRID-ORCS 1 BioMuta 1 CCDS 6 CDD 1 CTD 1 ChiTaRS 1 DNASU 1 DisGeNET 1 EMBL 12 Ensembl 9 ExpressionAtlas 1 GO 5 Gene3D 2 GeneCards 1 GeneTree 1 GenomeRNAi 1 HGNC 1 HOGENOM 1 HPA 1 InParanoid 1 IntAct 1 InterPro 5 KEGG 1 MANE-Select 1 MIM 1 MassIVE 1 NCBI Taxonomy 1 OMA 1 OpenTargets 1 OrthoDB 1 PANTHER 2 PRINTS 1 PRO 1 PROSITE 3 PathwayCommons 1 PaxDb 1 PeptideAtlas 1 Pfam 3 PharmGKB 1 Pharos 1 PhosphoSitePlus 1 PhylomeDB 1 Proteomes 1 ProteomicsDB 6 Pumba 1 REPRODUCTION-2DPAGE 1 RNAct 1 Reactome 1 RefSeq 10 SMART 2 SMR 1 STRING 1 SUPFAM 2 SignaLink 1 TreeFam 1 UCSC 1 VEuPathDB 1 eggNOG 1 iPTMnet 1 jPOST 1 neXtProt 1

Citations:

PubMed ID: 9344677

Title: Characterization of a KRAB family zinc finger gene, ZNF195, mapping to chromosome band 11p15.5.

PubMed ID: 9344677

DOI: 10.1006/geno.1997.4958

PubMed ID: 14702039

Title: Complete sequencing and characterization of 21,243 full-length human cDNAs.

PubMed ID: 14702039

DOI: 10.1038/ng1285

PubMed ID: 17974005

Title: The full-ORF clone resource of the German cDNA consortium.

PubMed ID: 17974005

DOI: 10.1186/1471-2164-8-399

PubMed ID: 16554811

Title: Human chromosome 11 DNA sequence and analysis including novel gene identification.

PubMed ID: 16554811

DOI: 10.1038/nature04632

PubMed ID: 15489334

Title: The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC).

PubMed ID: 15489334

DOI: 10.1101/gr.2596504

PubMed ID: 19608861

Title: Lysine acetylation targets protein complexes and co-regulates major cellular functions.

PubMed ID: 19608861

DOI: 10.1126/science.1175371

PubMed ID: 25218447

Title: Uncovering global SUMOylation signaling networks in a site-specific manner.

PubMed ID: 25218447

DOI: 10.1038/nsmb.2890

PubMed ID: 28112733

Title: Site-specific mapping of the human SUMO proteome reveals co-modification with phosphorylation.

PubMed ID: 28112733

DOI: 10.1038/nsmb.3366

Sequence Information:

  • Length: 629
  • Mass: 72332
  • Checksum: D048217DFC6BFDFB
  • Sequence:
    • MTLLTFRDVA IEFSLEEWKC LDLAQQNLYR DVMLENYRNL FSVGLTVCKP GLITCLEQRK 
EPWNVKRQEA ADGHPEMGFH HATQACLELL GSSDLPASAS QSAGITGVNH RAQPGLNVSV 
DKFTALCSPG VLQTVKWFLE FRCIFSLAMS SHFTQDLLPE QGIQDAFPKR ILRGYGNCGL 
DNLYLRKDWE SLDECKLQKD YNGLNQCSST THSKIFQYNK YVKIFDNFSN LHRRNISNTG 
EKPFKCQECG KSFQMLSFLT EHQKIHTGKK FQKCGECGKT FIQCSHFTEP ENIDTGEKPY 
KCQECNNVIK TCSVLTKNRI YAGGEHYRCE EFGKVFNQCS HLTEHEHGTE EKPCKYEECS 
SVFISCSSLS NQQMILAGEK LSKCETWYKG FNHSPNPSKH QRNEIGGKPF KCEECDSIFK 
WFSDLTKHKR IHTGEKPYKC DECGKAYTQS SHLSEHRRIH TGEKPYQCEE CGKVFRTCSS 
LSNHKRTHSE EKPYTCEECG NIFKQLSDLT KHKKTHTGEK PYKCDECGKN FTQSSNLIVH 
KRIHTGEKPY KCEECGRVFM WFSDITKHKK THTGEKPYKC DECGKNFTQS SNLIVHKRIH 
TGEKPYKCEK CGKAFTQFSH LTVHESIHT